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Protein

Lipoxygenase 3, chloroplastic

Gene

LOX3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

13S-lipoxygenase that can use linolenic acid as substrates. Plant lipoxygenases may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure (By similarity).By similarity1 Publication

Catalytic activityi

Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit.PROSITE-ProRule annotation

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi578Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi583Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi770Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi774Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi919Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • linoleate 13S-lipoxygenase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • anther dehiscence Source: TAIR
  • anther development Source: TAIR
  • lipid oxidation Source: TAIR
  • oxylipin biosynthetic process Source: UniProtKB-UniPathway
  • pollen development Source: TAIR
  • response to fungus Source: TAIR
  • response to high light intensity Source: UniProtKB
  • response to jasmonic acid Source: UniProtKB
  • response to wounding Source: UniProtKB
  • stamen filament development Source: TAIR

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.12 399
ReactomeiR-ATH-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-ATH-2142696 Synthesis of Hepoxilins (HX) and Trioxilins (TrX)
R-ATH-2142700 Synthesis of Lipoxins (LX)
R-ATH-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
R-ATH-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
R-ATH-6798695 Neutrophil degranulation
UniPathwayiUPA00382

Chemistry databases

SwissLipidsiSLP:000001766

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoxygenase 3, chloroplastic (EC:1.13.11.12)
Short name:
AtLOX3
Gene namesi
Name:LOX3
Ordered Locus Names:At1g17420
ORF Names:F28G4.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G17420
TAIRilocus:2018848 AT1G17420

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 52ChloroplastSequence analysisAdd BLAST52
ChainiPRO_000038059253 – 919Lipoxygenase 3, chloroplasticAdd BLAST867

Proteomic databases

PaxDbiQ9LNR3

Expressioni

Tissue specificityi

Expressed in roots and leaves.1 Publication

Developmental stagei

First observed in lateral root primordia (LRP), from the first pericycle divisions. Later expressed in lateral roots. Expression is greatly increased in leaves during leaf senescence.2 Publications

Inductioni

Induced by methyl jasmonate (MeJA), bacterial pathogens (e.g. Pseudomonas syringae pv. tomato), high light and wounding.3 Publications

Gene expression databases

ExpressionAtlasiQ9LNR3 baseline and differential
GenevisibleiQ9LNR3 AT

Interactioni

Protein-protein interaction databases

BioGridi23554, 2 interactors
IntActiQ9LNR3, 1 interactor
STRINGi3702.AT1G17420.1

Structurei

3D structure databases

ProteinModelPortaliQ9LNR3
SMRiQ9LNR3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 222PLATPROSITE-ProRule annotationAdd BLAST137
Domaini225 – 919LipoxygenasePROSITE-ProRule annotationAdd BLAST695

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IEKP Eukaryota
ENOG410YN4N LUCA
HOGENOMiHOG000230469
InParanoidiQ9LNR3
KOiK00454
OMAiSQKDHPE
OrthoDBiEOG0936038B
PhylomeDBiQ9LNR3

Family and domain databases

Gene3Di4.10.372.10, 1 hit
InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001246 LipOase_plant
IPR027433 Lipoxygenase_dom_3
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00468 PLTLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LNR3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALAKELMGY PLITERSSLV SSASHFKKRT QSTQFSINPF DRRPRKTKSG
60 70 80 90 100
VVAAISEDLV KTLRFSTTTG DRKSEEEEKA AVKFKVRAVV TVRNKNKEDL
110 120 130 140 150
KETLVKHLDA FADKIGRNIV LELISTQLDP KTKLPKKSNA AVLKDWSKKS
160 170 180 190 200
KTKAERVHYT AEFTVDAAFG SPGAITVMNK HQKEFFLESI TIEGFALGPV
210 220 230 240 250
HFPCNSWVQS QKDHPDKRIF FTNQPYLPNE TPSGLRVLRE KELKNLRGDG
260 270 280 290 300
SGVRKLSDRI YDFDVYNDLG NPDKSSELSR PKLGGKEVPY PRRCRTGRQS
310 320 330 340 350
TVSDKDAESR VEKPLPMYVP RDEQFEESKQ DTFAAGRLKA VLHHLIPSLK
360 370 380 390 400
ASIVAEDFAD FGEIDRLYKE GLLLKLGFQD DIFKKFPLPK VVVDTLQEST
410 420 430 440 450
KGLLKYDTPK ILSKDKNAWL RDDEFARQAI AGINPVNIER VKTFPPVSNL
460 470 480 490 500
DPKIYGPQHS ALTDDHIIGH LDGFSVQQAL EENRLYMLDY HDIFLPFLDR
510 520 530 540 550
INALDGRKAY ATRTIFFLTR LGTLKPVAIE LSLPPHGPKH RSKRVLTPPV
560 570 580 590 600
DATSNWMWQL AKAHVSSNDA GVHQLVNHWL RTHACLEPFI LAAHRQLSAM
610 620 630 640 650
HPIFKLLDPH MRYTLEINAL ARQSLISADG VIEGGFTAGA YGMEMSAAAY
660 670 680 690 700
KSSWRFDMEG LPADLIRRGM AIPDATQPHG LKLLIEDYPY ANDGLLLWSA
710 720 730 740 750
IQTWVRTYVE RYYPNPNLIK TDSELQSWYS ESINVGHADL RDADWWPELS
760 770 780 790 800
TVDDLVSILT TLIWLASAQH AALNFGQYPY GGYVPNRPPL MRRLIPDESD
810 820 830 840 850
PEYASFISHP EKYYFSSMPS LAQTSKFMAV VDTLSTHSPD EEYIGERQQP
860 870 880 890 900
SIWTGDAEIV EAFYGFAAEI GRIEKEIEKR NADPDRRNRC GAGVLPYELL
910
VPSSEPGVTC RGVPNSVSI
Length:919
Mass (Da):103,726
Last modified:October 1, 2000 - v1
Checksum:i194A0B150273E44C
GO

Sequence cautioni

The sequence AAF97315 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti374L → P in CAB56692 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ249794 mRNA Translation: CAB56692.1
AC007843 Genomic DNA Translation: AAF97315.1 Different initiation.
AC022492 Genomic DNA Translation: AAF79461.1
CP002684 Genomic DNA Translation: AEE29585.1
AY075625 mRNA Translation: AAL91636.1
BT006348 mRNA Translation: AAP21156.1
RefSeqiNP_564021.1, NM_101603.3
UniGeneiAt.20467
At.64244
At.67022

Genome annotation databases

EnsemblPlantsiAT1G17420.1; AT1G17420.1; AT1G17420
GeneIDi838314
GrameneiAT1G17420.1; AT1G17420.1; AT1G17420
KEGGiath:AT1G17420

Similar proteinsi

Entry informationi

Entry nameiLOX3_ARATH
AccessioniPrimary (citable) accession number: Q9LNR3
Secondary accession number(s): Q9LQJ5, Q9SMW1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health