Reviewed, UniProtKB/Swiss-Prot Q9LNQ4 (ALA4_ARATH)

Names and origin

Protein names

Recommended name:
    Putative phospholipid-transporting ATPase 4
      Short name=AtALA4
    EC=3.6.3.1
Alternative name(s):
    Aminophospholipid flippase 4

Gene names

Name:	ALA4
Ordered Locus Names:	At1g17500
ORF Names:	F1L3.21

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1216 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in transport of phospholipids Potential.
Catalytic activity	ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IV subfamily.
Sequence caution	The sequence AAF79467.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro phospholipid transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid-translocating ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1216	1216	Putative phospholipid-transporting ATPase 4		PRO_0000046388
Regions
	Topological domain	1 – 74	74	Cytoplasmic Potential
	Transmembrane	75 – 96	22	Potential
	Topological domain	97 – 100	4	Extracellular Potential
	Transmembrane	101 – 123	23	Potential
	Topological domain	124 – 305	182	Cytoplasmic Potential
	Transmembrane	306 – 327	22	Potential
	Topological domain	328 – 359	32	Extracellular Potential
	Transmembrane	360 – 377	18	Potential
	Topological domain	378 – 922	545	Cytoplasmic Potential
	Transmembrane	923 – 942	20	Potential
	Topological domain	943 – 956	14	Extracellular Potential
	Transmembrane	957 – 976	20	Potential
	Topological domain	977 – 1006	30	Cytoplasmic Potential
	Transmembrane	1007 – 1029	23	Potential
	Topological domain	1030 – 1042	13	Extracellular Potential
	Transmembrane	1043 – 1065	23	Potential
	Topological domain	1066 – 1071	6	Cytoplasmic Potential
	Transmembrane	1072 – 1092	21	Potential
	Topological domain	1093 – 1109	17	Extracellular Potential
	Transmembrane	1110 – 1134	25	Potential
	Topological domain	1135 – 1216	82	Cytoplasmic Potential
Sites
	Active site	425	1	4-aspartylphosphate intermediate By similarity
	Metal binding	867	1	Magnesium By similarity
	Metal binding	871	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LNQ4-1 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: FC9B0CD7F96D21E1	FASTA	1,216	138,189
	10 20 30 40 50 60 MARGRIRSKL RLSHIYTFGC LRPSADEGQD PHPIQGPGFS RTVYCNQPHM HKKKPLKYRS 70 80 90 100 110 120 NYVSTTRYNL ITFFPKCLYE QFHRAANFYF LVAAILSVFP LSPFNKWSMI APLVFVVGLS 130 140 150 160 170 180 MLKEALEDWS RFMQDVKINA SKVYVHKSDG EFRRRKWKKI SVGDIVKVEK DGFFPADLLL 190 200 210 220 230 240 LSSSYEDGIC YVETMNLDGE TNLKVKRSLE VTLSLDDYDS FKDFTGIIRC EDPNPSLYTF 250 260 270 280 290 300 VGNLEYERQI FPLDPSQILL RDSKLRNTPY VYGVVVFTGH DTKVMQNSTK SPSKRSRIEK 310 320 330 340 350 360 TMDYIIYTLL VLLILISCIS SSGFAWETKF HMPKWWYLRP EEPENLTNPS NPVYAGFVHL 370 380 390 400 410 420 ITALLLYGYL IPISLYVSIE VVKVLQASFI NKDLHMYDSE SGVPAHARTS NLNEELGQVD 430 440 450 460 470 480 TILSDKTGTL TCNQMDFLKC SIAGTSYGVR SSEVEVAAAQ QMAVDLDEHG EVSSRTSTPR 490 500 510 520 530 540 AQARDIEVES SITPRIPIKG FGFEDIRLMD GNWLREPHTD DILLFFRILA ICHTAIPELN 550 560 570 580 590 600 EETGKYTYEA ESPDEASFLT AASEFGFVFF KRTQSSVYVH ERLSHSGQTI EREYKVLNLL 610 620 630 640 650 660 DFTSKRKRMS VVVRDEEGQI LLLCKGADSI IFERLAKNGK VYLGPTTKHL NEYGEAGLRT 670 680 690 700 710 720 LALSYRKLDE EEYSAWNAEF HKAKTSIGSD RDELLERISD MIEKDLILVG ATAVEDKLQK 730 740 750 760 770 780 GVPQCIDKLA QAGLKLWVLT GDKMETAINI GYSCSLLRQG MKQICITVVN SEGASQDAKA 790 800 810 820 830 840 VKDNILNQIT KAVQMVKLEK DPHAAFALII DGKTLTYALE DEMKYQFLAL AVDCASVICC 850 860 870 880 890 900 RVSPKQKALV TRLVKEGTGK ITLAIGDGAN DVGMIQEADI GVGISGVEGM QAVMASDFSI 910 920 930 940 950 960 AQFRFLERLL VVHGHWCYKR IAQMICYFFY KNIAFGLTLF YFEAFTGFSG QSVYNDYYLL 970 980 990 1000 1010 1020 LFNVVLTSLP VIALGVFEQD VSSEICLQFP ALYQQGKKNL FFDWYRILGW MGNGVYSSLV 1030 1040 1050 1060 1070 1080 IFFLNIGIIY EQAFRVSGQT ADMDAVGTTM FTCIIWAVNV QIALTVSHFT WIQHVLIWGS 1090 1100 1110 1120 1130 1140 IGLWYLFVAL YGMMPPSLSG NIYRILVEIL APAPIYWIAT FLVTVTTVLP YFAHISFQRF 1150 1160 1170 1180 1190 1200 LHPLDHHIIQ EIKYYKRDVE DRRMWTRERT KAREKTKIGF TARVDAKIRH LRSKLNKKQS 1210 NMSQFSTQDT MSPRSV

References

[1]

"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC022492 Genomic DNA. Translation: AAF79467.1. Sequence problems.
IPI	IPI00531539.
RefSeq	NP_173193.2.
UniGene	At.27975
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9LNQ4.
Genome annotation databases
GeneID	838324.
GenomeReviews	Gene locus AT1G17500 in contig CT485782_GR.
KEGG	ath:AT1G17500.
Organism-specific databases
TAIR	At1g17500.
Enzyme and pathway databases
BRENDA	3.6.3.1. 302.
Gene expression databases
ArrayExpress	Q9LNQ4.
GermOnline	AT1G17500. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-reg. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_phosphor_site. IPR006539. ATPase_P-typ_Plipid-transl. IPR005834. Dehalogen-like_hydro. IPR013200. HAD-SF_hydro-like_3. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. PF08282. Hydrolase_3. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
TIGRFAMs	TIGR01652. ATPase-Plipid. 1 hit. TIGR01494. ATPase_P-type. 4 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALA4_ARATH

Accession

Primary (citable) accession number: Q9LNQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 11, 2001
Last modified:	June 16, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)