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Protein

Protein phosphatase 2C 7

Gene

HAB2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Key component and repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, seed germination and inhibition of vegetative growth.By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi242 – 2421Manganese 1By similarity
Metal bindingi242 – 2421Manganese 2By similarity
Metal bindingi243 – 2431Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi432 – 4321Manganese 2By similarity
Metal bindingi492 – 4921Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: InterPro

GO - Biological processi

  1. abscisic acid-activated signaling pathway Source: UniProtKB-KW
  2. protein dephosphorylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Abscisic acid signaling pathway

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G17550-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C 7 (EC:3.1.3.16)
Short name:
AtPP2C07
Alternative name(s):
Protein HYPERSENSITIVE TO ABA 2
Protein phosphatase 2C HAB2
Short name:
PP2C HAB2
Gene namesi
Name:HAB2
Ordered Locus Names:At1g17550
ORF Names:F1L3.32
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G17550.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 511492Protein phosphatase 2C 7PRO_0000344525Add
BLAST

Proteomic databases

PaxDbiQ9LNP9.
PRIDEiQ9LNP9.

Expressioni

Tissue specificityi

Expressed in seeds.1 Publication

Inductioni

Repressed by MYB44. Induced by ABA.3 Publications

Gene expression databases

GenevestigatoriQ9LNP9.

Interactioni

Protein-protein interaction databases

BioGridi23570. 2 interactions.
DIPiDIP-48989N.

Structurei

3D structure databases

SMRiQ9LNP9. Positions 179-510.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini188 – 501314PPM-type phosphatasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000233896.
InParanoidiQ9LNP9.
KOiK14497.
OMAiWMLTGNN.
PhylomeDBiQ9LNP9.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LNP9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEISPAVAL TLGLANTMCD SGISSTFDIS ELENVTDAAD MLCNQKRQRY
60 70 80 90 100
SNGVVDCIMG SVSEEKTLSE VRSLSSDFSV TVQESEEDEP LVSDATIISE
110 120 130 140 150
GLIVVDARSE ISLPDTVETD NGRVLATAII LNETTIEQVP TAEVLIASLN
160 170 180 190 200
HDVNMEVATS EVVIRLPEEN PNVARGSRSV YELECIPLWG TISICGGRSE
210 220 230 240 250
MEDAVRALPH FLKIPIKMLM GDHEGMSPSL PYLTSHFFGV YDGHGGAQVA
260 270 280 290 300
DYCHDRIHSA LAEEIERIKE ELCRRNTGEG RQVQWEKVFV DCYLKVDDEV
310 320 330 340 350
KGKINRPVVG SSDRMVLEAV SPETVGSTAV VALVCSSHII VSNCGDSRAV
360 370 380 390 400
LLRGKDSMPL SVDHKPDRED EYARIEKAGG KVIQWQGARV SGVLAMSRSI
410 420 430 440 450
GDQYLEPFVI PDPEVTFMPR AREDECLILA SDGLWDVMSN QEACDFARRR
460 470 480 490 500
ILAWHKKNGA LPLAERGVGE DQACQAAAEY LSKLAIQMGS KDNISIIVID
510
LKAQRKFKTR S
Length:511
Mass (Da):56,062
Last modified:July 22, 2008 - v2
Checksum:i167CB9D9D5FF9A59
GO

Sequence cautioni

The sequence AAF79469.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti475 – 4751Q → R in BAC43252 (PubMed:11910074).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC022492 Genomic DNA. Translation: AAF79469.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29605.1.
AK118656 mRNA. Translation: BAC43252.1.
RefSeqiNP_173199.2. NM_101618.2.
UniGeneiAt.41827.

Genome annotation databases

EnsemblPlantsiAT1G17550.1; AT1G17550.1; AT1G17550.
GeneIDi838330.
KEGGiath:AT1G17550.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC022492 Genomic DNA. Translation: AAF79469.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29605.1.
AK118656 mRNA. Translation: BAC43252.1.
RefSeqiNP_173199.2. NM_101618.2.
UniGeneiAt.41827.

3D structure databases

SMRiQ9LNP9. Positions 179-510.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23570. 2 interactions.
DIPiDIP-48989N.

Proteomic databases

PaxDbiQ9LNP9.
PRIDEiQ9LNP9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G17550.1; AT1G17550.1; AT1G17550.
GeneIDi838330.
KEGGiath:AT1G17550.

Organism-specific databases

TAIRiAT1G17550.

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000233896.
InParanoidiQ9LNP9.
KOiK14497.
OMAiWMLTGNN.
PhylomeDBiQ9LNP9.

Enzyme and pathway databases

BioCyciARA:AT1G17550-MONOMER.

Gene expression databases

GenevestigatoriQ9LNP9.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Gain-of-function and loss-of-function phenotypes of the protein phosphatase 2C HAB1 reveal its role as a negative regulator of abscisic acid signalling."
    Saez A., Apostolova N., Gonzalez-Guzman M., Gonzalez-Garcia M.P., Nicolas C., Lorenzo O., Rodriguez P.L.
    Plant J. 37:354-369(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY ABA.
  5. "Plant PP2C phosphatases: emerging functions in stress signaling."
    Schweighofer A., Hirt H., Meskiene I.
    Trends Plant Sci. 9:236-243(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "ABA-hypersensitive germination3 encodes a protein phosphatase 2C (AtPP2CA) that strongly regulates abscisic acid signaling during germination among Arabidopsis protein phosphatase 2Cs."
    Yoshida T., Nishimura N., Kitahata N., Kuromori T., Ito T., Asami T., Shinozaki K., Hirayama T.
    Plant Physiol. 140:115-126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY ABA, TISSUE SPECIFICITY.
  7. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  8. "Overexpression of AtMYB44 enhances stomatal closure to confer abiotic stress tolerance in transgenic Arabidopsis."
    Jung C., Seo J.S., Han S.W., Koo Y.J., Kim C.H., Song S.I., Nahm B.H., Choi Y.D., Cheong J.-J.
    Plant Physiol. 146:623-635(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY MYB44.

Entry informationi

Entry nameiP2C07_ARATH
AccessioniPrimary (citable) accession number: Q9LNP9
Secondary accession number(s): Q8GWS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: July 22, 2008
Last modified: April 1, 2015
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.