Q9LNP9 (P2C07_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 2C 7
Short name=AtPP2C07
EC=3.1.3.16

Alternative name(s):
Protein HYPERSENSITIVE TO ABA 2
Protein phosphatase 2C HAB2
Short name=PP2C HAB2

Gene names

Name:	HAB2
Ordered Locus Names:	At1g17550
ORF Names:	F1L3.32

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Key component and repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, seed germination and inhibition of vegetative growth By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Tissue specificity	Expressed in seeds. Ref.6
Induction	Repressed by MYB44. Induced by ABA. Ref.4 Ref.6 Ref.8
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.
Sequence caution	The sequence AAF79469.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Abscisic acid signaling pathway
Domain	Signal
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	abscisic acid mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW protein dephosphorylation Inferred from electronic annotation. Source: InterPro
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 511	492	Protein phosphatase 2C 7		PRO_0000344525
Regions
	Domain	187 – 494	308	PP2C-like
Sites
	Metal binding	242	1	Manganese 1 By similarity
	Metal binding	242	1	Manganese 2 By similarity
	Metal binding	243	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	432	1	Manganese 2 By similarity
	Metal binding	492	1	Manganese 2 By similarity
Experimental info
	Sequence conflict	475	1	Q → R in BAC43252. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9LNP9 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: 167CB9D9D5FF9A59	FASTA	511	56,062
	10 20 30 40 50 60 MEEISPAVAL TLGLANTMCD SGISSTFDIS ELENVTDAAD MLCNQKRQRY SNGVVDCIMG 70 80 90 100 110 120 SVSEEKTLSE VRSLSSDFSV TVQESEEDEP LVSDATIISE GLIVVDARSE ISLPDTVETD 130 140 150 160 170 180 NGRVLATAII LNETTIEQVP TAEVLIASLN HDVNMEVATS EVVIRLPEEN PNVARGSRSV 190 200 210 220 230 240 YELECIPLWG TISICGGRSE MEDAVRALPH FLKIPIKMLM GDHEGMSPSL PYLTSHFFGV 250 260 270 280 290 300 YDGHGGAQVA DYCHDRIHSA LAEEIERIKE ELCRRNTGEG RQVQWEKVFV DCYLKVDDEV 310 320 330 340 350 360 KGKINRPVVG SSDRMVLEAV SPETVGSTAV VALVCSSHII VSNCGDSRAV LLRGKDSMPL 370 380 390 400 410 420 SVDHKPDRED EYARIEKAGG KVIQWQGARV SGVLAMSRSI GDQYLEPFVI PDPEVTFMPR 430 440 450 460 470 480 AREDECLILA SDGLWDVMSN QEACDFARRR ILAWHKKNGA LPLAERGVGE DQACQAAAEY 490 500 510 LSKLAIQMGS KDNISIIVID LKAQRKFKTR S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Gain-of-function and loss-of-function phenotypes of the protein phosphatase 2C HAB1 reveal its role as a negative regulator of abscisic acid signalling." Saez A., Apostolova N., Gonzalez-Guzman M., Gonzalez-Garcia M.P., Nicolas C., Lorenzo O., Rodriguez P.L. Plant J. 37:354-369(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY ABA.
[5]	"Plant PP2C phosphatases: emerging functions in stress signaling." Schweighofer A., Hirt H., Meskiene I. Trends Plant Sci. 9:236-243(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"ABA-hypersensitive germination3 encodes a protein phosphatase 2C (AtPP2CA) that strongly regulates abscisic acid signaling during germination among Arabidopsis protein phosphatase 2Cs." Yoshida T., Nishimura N., Kitahata N., Kuromori T., Ito T., Asami T., Shinozaki K., Hirayama T. Plant Physiol. 140:115-126(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY ABA, TISSUE SPECIFICITY.
[7]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[8]	"Overexpression of AtMYB44 enhances stomatal closure to confer abiotic stress tolerance in transgenic Arabidopsis." Jung C., Seo J.S., Han S.W., Koo Y.J., Kim C.H., Song S.I., Nahm B.H., Choi Y.D., Cheong J.-J. Plant Physiol. 146:623-635(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY MYB44.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC022492 Genomic DNA. Translation: AAF79469.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE29605.1. AK118656 mRNA. Translation: BAC43252.1.
IPI	IPI00532752.
RefSeq	NP_173199.2. NM_101618.2.
UniGene	At.41827.
3D structure databases
HSSP	HSSP built from PDB template 1A6Q based on UniProtKB P35813.
ProteinModelPortal	Q9LNP9.
SMR	Q9LNP9. Positions 179-510.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48989N.
Proteomic databases
PaxDb	Q9LNP9.
PRIDE	Q9LNP9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G17550.1; AT1G17550.1; AT1G17550.
GeneID	838330.
KEGG	ath:AT1G17550.
Organism-specific databases
TAIR	At1g17550.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233896.
InParanoid	Q8GWS8.
KO	K14497.
OMA	RRILAWH.
PhylomeDB	Q9LNP9.
ProtClustDB	CLSN2679602.
Gene expression databases
Genevestigator	Q9LNP9.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C07_ARATH

Accession

Primary (citable) accession number: Q9LNP9
Secondary accession number(s): Q8GWS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	May 1, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program