Reviewed, UniProtKB/Swiss-Prot Q9LNJ0 (CNG10_ARATH)

Names and origin

Protein names

Recommended name:
    Probable cyclic nucleotide-gated ion channel 10
Alternative name(s):
    Cyclic nucleotide- and calmodulin-regulated ion channel 10
    CaM-regulated potassium ion channel

Gene names

Name:	CNGC10
Synonyms:	ACBK1
Ordered Locus Names:	At1g01340
ORF Names:	F6F3.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	711 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable cyclic nucleotide-gated ion channel.
Subunit structure	Homotetramer or heterotetramer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Domain	The binding of calmodulin to the C-terminus might interfere with cyclic nucleotide binding and thus channel activation By similarity.
Sequence similarities	Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. [View classification] Contains 1 cyclic nucleotide-binding domain. Contains 1 IQ domain.
Sequence caution	The sequence AAF97331.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Ligand	Calmodulin-binding Nucleotide-binding cAMP cAMP-binding cGMP cGMP-binding
Molecular function	Ionic channel
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ion transport Inferred from electronic annotation. Source: UniProtKB-KW transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cAMP binding Inferred from electronic annotation. Source: UniProtKB-KW cGMP binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9LNJ0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Constructed according to the conserved gene model. No experimental confirmation available.

Isoform 2 (identifier: Q9LNJ0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MAFSHDNRV → MILF

Note: May be due to an intron retention. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 711	711	Probable cyclic nucleotide-gated ion channel 10		PRO_0000219338
Regions
	Topological domain	1 – 81	81	Cytoplasmic Potential
	Transmembrane	82 – 102	21	H1 Potential
	Topological domain	103 – 116	14	Extracellular Potential
	Transmembrane	117 – 137	21	H2 Potential
	Topological domain	138 – 170	33	Cytoplasmic Potential
	Transmembrane	171 – 191	21	H3 Potential
	Topological domain	192 – 204	13	Extracellular Potential
	Transmembrane	205 – 225	21	H4 Potential
	Topological domain	226 – 243	18	Cytoplasmic Potential
	Transmembrane	244 – 264	21	H5 Potential
	Topological domain	265 – 366	102	Extracellular Potential
	Transmembrane	367 – 387	21	H6 Potential
	Topological domain	388 – 711	324	Cytoplasmic Potential
	Domain	609 – 638	30	IQ
	Nucleotide binding	473 – 603	131	cNMP
	Region	589 – 604	16	Calmodulin-binding By similarity
Sites
	Binding site	544	1	cNMP By similarity
Natural variations
	Alternative sequence	1 – 9	9	MAFSHDNRV → MILF in isoform 2.		VSP_008988

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 21, 2003. Version 2. Checksum: D38AA46664B50928	FASTA	711	81,895
	10 20 30 40 50 60 MAFSHDNRVR FKDEGKPLSS EYGYGRKARP SLDRVFKNVK WGFKKPLSFP SHKDPDHKET 70 80 90 100 110 120 SSVTRKNIIN PQDSFLQNWN KIFLFACVVA LAIDPLFFYI PIVDSARHCL TLDSKLEIAA 130 140 150 160 170 180 SLLRTLIDAF YIIHIVFQFR TAYIAPSSRV FGRGELVDDA KAIALKYLSS YFIIDLLSIL 190 200 210 220 230 240 PLPQIVVLAV IPSVNQPVSL LTKDYLKFSI IAQYVPRILR MYPLYTEVTR TSGIVTETAW 250 260 270 280 290 300 AGAAWNLSLY MLASHVFGAL WYLISVERED RCWQEACEKT KGCNMKFLYC ENDRNVSNNF 310 320 330 340 350 360 LTTSCPFLDP GDITNSTIFN FGIFTDALKS GVVESHDFWK KFFYCFWWGL RNLSALGQNL 370 380 390 400 410 420 QTSKFVGEII FAISICISGL VLFALLIGNM QKYLESTTVR EEEMRVRKRD AEQWMSHRML 430 440 450 460 470 480 PEDLRKRIRR YEQYRWQETR GVEEETLLRN LPKDLRRDIK RHLCLDLLKK VPLFEIMDEQ 490 500 510 520 530 540 LLDAVCDRLR PVLYTENSYV IREGDPVGEM LFVMRGRLVS ATTNGGRSGF FNAVNLKASD 550 560 570 580 590 600 FCGEDLLPWA LDPQSSSHFP ISTRTVQALT EVEAFALTAE DLKSVASQFR RLHSKQLQHT 610 620 630 640 650 660 FRFYSVQWRT WSVSFIQAAW RRYCRRKLAK SLRDEEDRLR EALASQDKEH NAATVSSSLS 670 680 690 700 710 LGGALYASRF ASNALHNLRH NISNLPPRYT LPLLPQKPTE PDFTANHTTD P
	Isoform 2. Checksum: B6B18153E4644050	FASTA	706	81,342
	10 20 30 40 50 60 MILFRFKDEG KPLSSEYGYG RKARPSLDRV FKNVKWGFKK PLSFPSHKDP DHKETSSVTR 70 80 90 100 110 120 KNIINPQDSF LQNWNKIFLF ACVVALAIDP LFFYIPIVDS ARHCLTLDSK LEIAASLLRT 130 140 150 160 170 180 LIDAFYIIHI VFQFRTAYIA PSSRVFGRGE LVDDAKAIAL KYLSSYFIID LLSILPLPQI 190 200 210 220 230 240 VVLAVIPSVN QPVSLLTKDY LKFSIIAQYV PRILRMYPLY TEVTRTSGIV TETAWAGAAW 250 260 270 280 290 300 NLSLYMLASH VFGALWYLIS VEREDRCWQE ACEKTKGCNM KFLYCENDRN VSNNFLTTSC 310 320 330 340 350 360 PFLDPGDITN STIFNFGIFT DALKSGVVES HDFWKKFFYC FWWGLRNLSA LGQNLQTSKF 370 380 390 400 410 420 VGEIIFAISI CISGLVLFAL LIGNMQKYLE STTVREEEMR VRKRDAEQWM SHRMLPEDLR 430 440 450 460 470 480 KRIRRYEQYR WQETRGVEEE TLLRNLPKDL RRDIKRHLCL DLLKKVPLFE IMDEQLLDAV 490 500 510 520 530 540 CDRLRPVLYT ENSYVIREGD PVGEMLFVMR GRLVSATTNG GRSGFFNAVN LKASDFCGED 550 560 570 580 590 600 LLPWALDPQS SSHFPISTRT VQALTEVEAF ALTAEDLKSV ASQFRRLHSK QLQHTFRFYS 610 620 630 640 650 660 VQWRTWSVSF IQAAWRRYCR RKLAKSLRDE EDRLREALAS QDKEHNAATV SSSLSLGGAL 670 680 690 700 YASRFASNAL HNLRHNISNL PPRYTLPLLP QKPTEPDFTA NHTTDP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CaM-regulated potassium ion channel." Li X., Dharmasiri M.A., Harrington M.H. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Phylogenetic relationships within cation transporter families of Arabidopsis." Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H., Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F., Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L. Plant Physiol. 126:1646-1667(2001) [PubMed: 11500563] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF272002 mRNA. Translation: AAF76224.3. AC023628 Genomic DNA. Translation: AAF97331.1. Sequence problems.
IPI	IPI00532287. IPI00544133.
PIR	F86143.
RefSeq	NP_563625.1.
UniGene	At.15866
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	838832.
GenomeReviews	Gene locus AT1G01340 in contig CT485782_GR.
Organism-specific databases
GeneFarm	2771. 209.
TAIR	At1g01340.
Phylogenomic databases
OMA	ISICISG
Gene expression databases
ArrayExpress	Q9LNJ0.
Genevestigator	Q9LNJ0.
GermOnline	AT1G01340. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000595. cNMP_bd. IPR018490. cNMP_bd-like. IPR005821. Ion_trans. IPR000048. IQ_CaM_bd_region. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF00027. cNMP_binding. 1 hit. PF00520. Ion_trans. 1 hit. [Graphical view]
SMART	SM00100. cNMP. 1 hit. [Graphical view]
PROSITE	PS00888. CNMP_BINDING_1. False negative. PS00889. CNMP_BINDING_2. False negative. PS50042. CNMP_BINDING_3. 1 hit. PS50096. IQ. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CNG10_ARATH

Accession

Primary (citable) accession number: Q9LNJ0
Secondary accession number(s): Q9LKL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	November 21, 2003
Last modified:	November 24, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)