Reviewed, UniProtKB/Swiss-Prot Q9LND7 (SKI16_ARATH)

Names and origin

Protein names

Recommended name:
    F-box protein SKIP16
Alternative name(s):
    SKP1-interacting partner 16

Gene names

Name:	SKIP16
Ordered Locus Names:	At1g06110
ORF Names:	T21E18.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (ASK-cullin-F-box) protein ligase complex By similarity. Interacts with SKP1A/ASK1, SKP1B/ASK2, ASK4, ASK11 and ASK13.
Domain	The F-box is necessary for the interaction with ASK proteins By similarity.
Sequence similarities	Contains 1 apaG domain. Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	SCF ubiquitin ligase complex Ref.4 Inferred from physical interaction. Source: TAIR
Molecular function	protein binding Ref.4 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SKP1B	Q9FHW7	2	EBI-591078,EBI-604076

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	F-box protein SKIP16		PRO_0000375238
Regions
	Domain	75 – 111	37	F-box; degenerate
	Domain	295 – 436	142	ApaG

Sequences

	Sequence		Length	Mass (Da)
	Q9LND7-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 2FF2D4AAC5478259	FASTA	436	48,976
	10 20 30 40 50 60 MGLEDAGDLV LHIVLSKIGP ENTARVACVS KRLKVSASEE SLWSIFCSND LNISTPLDPH 70 80 90 100 110 120 GDPAPSFKRA YQLWRESFRM YPWNLVKRVR LCWDNLKQWL TLNFPEAKAT LRKGVTEDDL 130 140 150 160 170 180 QEFETSLKVK LPLPTRLLYR FVDGQELSSP NGLDGSLGLI GGYSAYSHDV NVYLLPLKEV 190 200 210 220 230 240 MRETKESFMR DLGFSSRLDL IVMAASVVAS LKIFLLDCTT GQLFTGTSNR QLLPCVPDAL 250 260 270 280 290 300 VRSVHDTNGD QQQDAMLLWL EEHGRRLQTG TINVRQQNNV KSISLFPEIP PLCSVSVTNG 310 320 330 340 350 360 VQVRASSVFI PEISNLRDQP PAYWYAYSIR MSLMPEGCIL NGTHHSSCQL YWRHWVIRAD 370 380 390 400 410 420 NEVIDNVNGE AVIGKYPLLQ AGEEEFVYES CSSFPTTAGS IDGSFTFVPG SLRDPKGSQF 430 EVKVVEFPLE LPDYIF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"The F-box subunit of the SCF E3 complex is encoded by a diverse superfamily of genes in Arabidopsis." Gagne J.M., Downes B.P., Shiu S.-H., Durski A.M., Vierstra R.D. Proc. Natl. Acad. Sci. U.S.A. 99:11519-11524(2002) [PubMed: 12169662] [Abstract] Cited for: INTERACTION WITH SKP1A/ASK1; SKP1B/ASK2; ASK4; ASK11 AND ASK13.
[4]	"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis." Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L. Plant J. 34:753-767(2003) [PubMed: 12795696] [Abstract] Cited for: INTERACTION WITH SKPIB/ASK2.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC024174 Genomic DNA. Translation: AAF80134.1. AF419599 mRNA. Translation: AAL31931.1. AY099571 mRNA. Translation: AAM20423.1. BT002142 mRNA. Translation: AAN72153.1.
IPI	IPI00545043.
PIR	D86196.
RefSeq	NP_563759.1.
UniGene	At.15468
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LND7. 5 interactions.
Proteomic databases
PRIDE	Q9LND7.
Genome annotation databases
GeneID	837120.
GenomeReviews	Gene locus AT1G06110 in contig CT485782_GR.
KEGG	ath:AT1G06110.
NMPDR	fig|3702.1.peg.780.
Organism-specific databases
TAIR	At1g06110.
Phylogenomic databases
OMA	Q9LND7. ACASTRL.
Gene expression databases
ArrayExpress	Q9LND7.
Family and domain databases
InterPro	IPR007474. ApaG. [Graphical view]
Gene3D	G3DSA:2.60.40.1470. ApaG. 1 hit.
Pfam	PF04379. DUF525. 1 hit. [Graphical view]
ProDom	PD031761. ApaG. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS51087. APAG. 1 hit. PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SKI16_ARATH

Accession

Primary (citable) accession number: Q9LND7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)