Q9LMT1 (P2C08_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 8
Short name=AtPP2C08
EC=3.1.3.16

Gene names

Ordered Locus Names:	At1g18030
ORF Names:	T10F20.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.
Sequence caution	The sequence AAF97840.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9LMT1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9LMT1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     301-305: FGPSD → SVWTK
     306-351: Missing.

Note: Derived from EST data. May be due to a competing donor splice site. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Probable protein phosphatase 2C 8		PRO_0000367939
Regions
	Domain	73 – 341	269	PP2C-like
Sites
	Metal binding	114	1	Manganese 1 By similarity
	Metal binding	114	1	Manganese 2 By similarity
	Metal binding	115	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	295	1	Manganese 2 By similarity
	Metal binding	339	1	Manganese 2 By similarity
Natural variations
	Alternative sequence	301 – 305	5	FGPSD → SVWTK in isoform 2.		VSP_036757
	Alternative sequence	306 – 351	46	Missing in isoform 2.		VSP_036758
Experimental info
	Sequence conflict	56	1	A → V in AAL16282. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 14, 2009. Version 2. Checksum: 0A6808FD713CCB7E	FASTA	351	38,486
	10 20 30 40 50 60 MLEKESDLTA MEKPNNKHAA DSFSSEDLVS PVKKAKKSEE VSGGGEAVAA VGNREAEEDK 70 80 90 100 110 120 PSFVSEEKKE FLVEADVAED KGARHTMEDV WVVLPDASLD FPGTLRCAHF AIYDGHGGRL 130 140 150 160 170 180 AAEFAKKHLH LNVLSAGLPR ELLDVKVAKK AILEGFRKTD ELLLQKSVSG GWQDGATAVC 190 200 210 220 230 240 VWILDQKVFV ANIGDAKAVL ARSSTTNELG NHTEAGNPLK AIVLTREHKA IYPQERSRIQ 250 260 270 280 290 300 KSGGVISSNG RLQGRLEVSR AFGDRHFKKF GVSATPDIHA FELTERENFM ILGCDGLWEV 310 320 330 340 350 FGPSDAVGFV QKLLKEGLHV STVSRRLVKE AVKERRCKDN CTAIVIVFKR V
	Isoform 2 [UniParc]. Checksum: 94E635FEEE8FA112	FASTA	305	33,377
	10 20 30 40 50 60 MLEKESDLTA MEKPNNKHAA DSFSSEDLVS PVKKAKKSEE VSGGGEAVAA VGNREAEEDK 70 80 90 100 110 120 PSFVSEEKKE FLVEADVAED KGARHTMEDV WVVLPDASLD FPGTLRCAHF AIYDGHGGRL 130 140 150 160 170 180 AAEFAKKHLH LNVLSAGLPR ELLDVKVAKK AILEGFRKTD ELLLQKSVSG GWQDGATAVC 190 200 210 220 230 240 VWILDQKVFV ANIGDAKAVL ARSSTTNELG NHTEAGNPLK AIVLTREHKA IYPQERSRIQ 250 260 270 280 290 300 KSGGVISSNG RLQGRLEVSR AFGDRHFKKF GVSATPDIHA FELTERENFM ILGCDGLWEV SVWTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Arabidopsis ORF clone." Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC034107 Genomic DNA. Translation: AAF97840.1. Different initiation. CP002684 Genomic DNA. Translation: AEE29665.1. CP002684 Genomic DNA. Translation: AEE29666.1. AF428352 mRNA. Translation: AAL16282.1. BT026023 mRNA. Translation: ABG48379.1.
IPI	IPI00541223. IPI00846926.
PIR	G86315.
RefSeq	NP_001077557.1. NM_001084088.1. NP_564045.1. NM_101665.2.
UniGene	At.14957. At.26247. At.26787. At.73166.
3D structure databases
HSSP	HSSP built from PDB template 1A6Q based on UniProtKB P35813.
ProteinModelPortal	Q9LMT1.
SMR	Q9LMT1. Positions 82-349.
ModBase	Search...
Proteomic databases
PaxDb	Q9LMT1.
PRIDE	Q9LMT1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G18030.1; AT1G18030.1; AT1G18030.
GeneID	838383.
KEGG	ath:AT1G18030.
Organism-specific databases
TAIR	At1g18030.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233896.
InParanoid	Q147S0.
OMA	WQDGATA.
PhylomeDB	Q9LMT1.
ProtClustDB	CLSN2687848.
Gene expression databases
Genevestigator	Q9LMT1.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C08_ARATH

Accession

Primary (citable) accession number: Q9LMT1
Secondary accession number(s): A8MRQ0, Q147S0, Q944K0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 14, 2009
Last modified:	May 1, 2013
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program