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Protein

Probable protein phosphatase 2C 8

Gene

At1g18030

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi114 – 1141Manganese 1By similarity
Metal bindingi114 – 1141Manganese 2By similarity
Metal bindingi115 – 1151Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi295 – 2951Manganese 2By similarity
Metal bindingi339 – 3391Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G18030-MONOMER.
ARA:GQT-968-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 8 (EC:3.1.3.16)
Short name:
AtPP2C08
Gene namesi
Ordered Locus Names:At1g18030
ORF Names:T10F20.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G18030.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Probable protein phosphatase 2C 8PRO_0000367939Add
BLAST

Proteomic databases

PaxDbiQ9LMT1.
PRIDEiQ9LMT1.

Expressioni

Gene expression databases

GenevestigatoriQ9LMT1.

Interactioni

Protein-protein interaction databases

BioGridi23622. 1 interaction.
IntActiQ9LMT1. 1 interaction.
MINTiMINT-8390724.

Structurei

3D structure databases

ProteinModelPortaliQ9LMT1.
SMRiQ9LMT1. Positions 82-349.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini74 – 348275PPM-type phosphatasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000233896.
InParanoidiQ9LMT1.
KOiK17500.
OMAiAVCVWIL.
PhylomeDBiQ9LMT1.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS51746. PPM_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LMT1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLEKESDLTA MEKPNNKHAA DSFSSEDLVS PVKKAKKSEE VSGGGEAVAA
60 70 80 90 100
VGNREAEEDK PSFVSEEKKE FLVEADVAED KGARHTMEDV WVVLPDASLD
110 120 130 140 150
FPGTLRCAHF AIYDGHGGRL AAEFAKKHLH LNVLSAGLPR ELLDVKVAKK
160 170 180 190 200
AILEGFRKTD ELLLQKSVSG GWQDGATAVC VWILDQKVFV ANIGDAKAVL
210 220 230 240 250
ARSSTTNELG NHTEAGNPLK AIVLTREHKA IYPQERSRIQ KSGGVISSNG
260 270 280 290 300
RLQGRLEVSR AFGDRHFKKF GVSATPDIHA FELTERENFM ILGCDGLWEV
310 320 330 340 350
FGPSDAVGFV QKLLKEGLHV STVSRRLVKE AVKERRCKDN CTAIVIVFKR

V
Length:351
Mass (Da):38,486
Last modified:April 14, 2009 - v2
Checksum:i0A6808FD713CCB7E
GO
Isoform 2 (identifier: Q9LMT1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     301-305: FGPSD → SVWTK
     306-351: Missing.

Note: Derived from EST data. May be due to a competing donor splice site. No experimental confirmation available.

Show »
Length:305
Mass (Da):33,377
Checksum:i94E635FEEE8FA112
GO

Sequence cautioni

The sequence AAF97840.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 561A → V in AAL16282 (PubMed:14593172).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei301 – 3055FGPSD → SVWTK in isoform 2. CuratedVSP_036757
Alternative sequencei306 – 35146Missing in isoform 2. CuratedVSP_036758Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC034107 Genomic DNA. Translation: AAF97840.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE29665.1.
CP002684 Genomic DNA. Translation: AEE29666.1.
AF428352 mRNA. Translation: AAL16282.1.
BT026023 mRNA. Translation: ABG48379.1.
PIRiG86315.
RefSeqiNP_001077557.1. NM_001084088.1. [Q9LMT1-2]
NP_564045.1. NM_101665.2. [Q9LMT1-1]
UniGeneiAt.14957.
At.26247.
At.26787.
At.73166.

Genome annotation databases

EnsemblPlantsiAT1G18030.1; AT1G18030.1; AT1G18030. [Q9LMT1-1]
GeneIDi838383.
KEGGiath:AT1G18030.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC034107 Genomic DNA. Translation: AAF97840.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE29665.1.
CP002684 Genomic DNA. Translation: AEE29666.1.
AF428352 mRNA. Translation: AAL16282.1.
BT026023 mRNA. Translation: ABG48379.1.
PIRiG86315.
RefSeqiNP_001077557.1. NM_001084088.1. [Q9LMT1-2]
NP_564045.1. NM_101665.2. [Q9LMT1-1]
UniGeneiAt.14957.
At.26247.
At.26787.
At.73166.

3D structure databases

ProteinModelPortaliQ9LMT1.
SMRiQ9LMT1. Positions 82-349.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23622. 1 interaction.
IntActiQ9LMT1. 1 interaction.
MINTiMINT-8390724.

Proteomic databases

PaxDbiQ9LMT1.
PRIDEiQ9LMT1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G18030.1; AT1G18030.1; AT1G18030. [Q9LMT1-1]
GeneIDi838383.
KEGGiath:AT1G18030.

Organism-specific databases

TAIRiAT1G18030.

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000233896.
InParanoidiQ9LMT1.
KOiK17500.
OMAiAVCVWIL.
PhylomeDBiQ9LMT1.

Enzyme and pathway databases

BioCyciARA:AT1G18030-MONOMER.
ARA:GQT-968-MONOMER.

Miscellaneous databases

PROiQ9LMT1.

Gene expression databases

GenevestigatoriQ9LMT1.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clone."
    Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiP2C08_ARATH
AccessioniPrimary (citable) accession number: Q9LMT1
Secondary accession number(s): A8MRQ0, Q147S0, Q944K0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: April 1, 2015
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.