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Protein

Wall-associated receptor kinase 2

Gene

WAK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase that may function as a signaling receptor of extracellular matrix component. Binding to pectin may have significance in the control of cell expansion, morphogenesis and development.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei432ATPPROSITE-ProRule annotation1
Active sitei529Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi410 – 418ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell surface receptor signaling pathway Source: GO_Central
  • cellular water homeostasis Source: TAIR
  • oligosaccharide metabolic process Source: TAIR
  • response to salicylic acid Source: TAIR
  • unidimensional cell growth Source: TAIR

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Calcium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wall-associated receptor kinase 2 (EC:2.7.11.-)
Gene namesi
Name:WAK2
Ordered Locus Names:At1g21270
ORF Names:F16F4.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G21270
TAIRilocus:2014912 AT1G21270

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 329ExtracellularSequence analysisAdd BLAST306
Transmembranei330 – 350HelicalSequence analysisAdd BLAST21
Topological domaini351 – 732CytoplasmicSequence analysisAdd BLAST382

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000025330124 – 732Wall-associated receptor kinase 2Add BLAST709

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi57N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi75N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi111N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi154N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi217N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi234 ↔ 249PROSITE-ProRule annotation
Disulfide bondi243 ↔ 260PROSITE-ProRule annotation
Glycosylationi246N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi262 ↔ 276PROSITE-ProRule annotation
Disulfide bondi282 ↔ 295PROSITE-ProRule annotation
Glycosylationi288N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi289 ↔ 304PROSITE-ProRule annotation
Disulfide bondi306 ↔ 318PROSITE-ProRule annotation
Modified residuei393PhosphothreonineBy similarity1
Modified residuei477PhosphotyrosineBy similarity1
Modified residuei563PhosphothreonineBy similarity1
Modified residuei568PhosphothreonineBy similarity1
Modified residuei576PhosphotyrosineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9LMP1
PRIDEiQ9LMP1

Expressioni

Tissue specificityi

Predominantly expressed in green tissues such as stems and leaves. Detected at organ junctions.1 Publication

Developmental stagei

Expressed in shoot and root apical meristems, and in expanding leaves and sepals.1 Publication

Inductioni

Induced by INA and wounding.2 Publications

Gene expression databases

ExpressionAtlasiQ9LMP1 baseline and differential
GenevisibleiQ9LMP1 AT

Interactioni

Protein-protein interaction databases

BioGridi23962, 6 interactors
IntActiQ9LMP1, 2 interactors
STRINGi3702.AT1G21270.1

Structurei

3D structure databases

ProteinModelPortaliQ9LMP1
SMRiQ9LMP1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini230 – 277EGF-like 1PROSITE-ProRule annotationAdd BLAST48
Domaini278 – 319EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST42
Domaini404 – 677Protein kinasePROSITE-ProRule annotationAdd BLAST274

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IM0I Eukaryota
COG0515 LUCA
HOGENOMiHOG000116550
InParanoidiQ9LMP1
OMAiTRTHVES
OrthoDBiEOG093603PB
PhylomeDBiQ9LMP1

Family and domain databases

InterProiView protein in InterPro
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008271 Ser/Thr_kinase_AS
IPR025287 WAK_GUB
PfamiView protein in Pfam
PF07645 EGF_CA, 1 hit
PF13947 GUB_WAK_bind, 1 hit
PF07714 Pkinase_Tyr, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 2 hits
SM00179 EGF_CA, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 1 hit
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 2 hits
PS01187 EGF_CA, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LMP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVQEGLFVV AVFYLAYTQL VKGQPRKECQ TRCGNVAVEY PFGTSPGCYY
60 70 80 90 100
PGDESFNLTC NEQEKLFFGN MPVINMSLSG QLRVRLVRSR VCYDSQGKQT
110 120 130 140 150
DYIAQRTTLG NFTLSELNRF TVVGCNSYAF LRTSGVEKYS TGCISICDSA
160 170 180 190 200
TTKNGSCSGE GCCQIPVPRG YSFVRVKPHS FHNHPTVHLF NPCTYAFLVE
210 220 230 240 250
DGMFDFHALE DLNNLRNVTT FPVVLDWSIG DKTCKQVEYR GVCGGNSTCF
260 270 280 290 300
DSTGGTGYNC KCLEGFEGNP YLPNGCQDIN ECISSRHNCS EHSTCENTKG
310 320 330 340 350
SFNCNCPSGY RKDSLNSCTR KVRPEYFRWT QIFLGTTIGF SVIMLGISCL
360 370 380 390 400
QQKIKHRKNT ELRQKFFEQN GGGMLIQRVS GAGPSNVDVK IFTEKGMKEA
410 420 430 440 450
TNGYHESRIL GQGGQGTVYK GILPDNSIVA IKKARLGNRS QVEQFINEVL
460 470 480 490 500
VLSQINHRNV VKVLGCCLET EVPLLVYEFI NSGTLFDHLH GSLYDSSLTW
510 520 530 540 550
EHRLRIATEV AGSLAYLHSS ASIPIIHRDI KTANILLDKN LTAKVADFGA
560 570 580 590 600
SRLIPMDKEQ LTTIVQGTLG YLDPEYYNTG LLNEKSDVYS FGVVLMELLS
610 620 630 640 650
GQKALCFERP HCPKNLVSCF ASATKNNRFH EIIDGQVMNE DNQREIQEAA
660 670 680 690 700
RIAAECTRLM GEERPRMKEV AAELEALRVK TTKYKWSDQY RETGEIEHLL
710 720 730
GVQILSAQGE TSSSIGYDSI RNVTTLDIEA GR
Length:732
Mass (Da):81,644
Last modified:October 1, 2000 - v1
Checksum:i5B1817EF8CA0BEF5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti105Q → R (PubMed:10380805).Curated1
Sequence conflicti105Q → R (Ref. 2) Curated1
Sequence conflicti545V → A (PubMed:10380805).Curated1
Sequence conflicti545V → A (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ012423 mRNA Translation: CAB42872.1
AF083722 mRNA Translation: AAN60280.1
AC036104 Genomic DNA Translation: AAF81355.1
CP002684 Genomic DNA Translation: AEE30080.1
AY062531 mRNA Translation: AAL32609.1
BT010335 mRNA Translation: AAQ56778.1
PIRiT52588
RefSeqiNP_173549.1, NM_101979.4
UniGeneiAt.21118
At.23880
At.69963

Genome annotation databases

EnsemblPlantsiAT1G21270.1; AT1G21270.1; AT1G21270
GeneIDi838723
GrameneiAT1G21270.1; AT1G21270.1; AT1G21270
KEGGiath:AT1G21270

Similar proteinsi

Entry informationi

Entry nameiWAK2_ARATH
AccessioniPrimary (citable) accession number: Q9LMP1
Secondary accession number(s): Q9XGN2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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