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Protein

Wall-associated receptor kinase 2

Gene

WAK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase that may function as a signaling receptor of extracellular matrix component. Binding to pectin may have significance in the control of cell expansion, morphogenesis and development.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei432 – 4321ATPPROSITE-ProRule annotation
Active sitei529 – 5291Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi410 – 4189ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cell surface receptor signaling pathway Source: GO_Central
  • cellular water homeostasis Source: TAIR
  • oligosaccharide metabolic process Source: TAIR
  • response to salicylic acid Source: TAIR
  • unidimensional cell growth Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G21270-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Wall-associated receptor kinase 2 (EC:2.7.11.-)
Gene namesi
Name:WAK2
Ordered Locus Names:At1g21270
ORF Names:F16F4.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G21270.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 329306ExtracellularSequence analysisAdd
BLAST
Transmembranei330 – 35021HelicalSequence analysisAdd
BLAST
Topological domaini351 – 732382CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell Source: GOC
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 732709Wall-associated receptor kinase 2PRO_0000253301Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...)Sequence analysis
Glycosylationi75 – 751N-linked (GlcNAc...)Sequence analysis
Glycosylationi111 – 1111N-linked (GlcNAc...)Sequence analysis
Glycosylationi154 – 1541N-linked (GlcNAc...)Sequence analysis
Glycosylationi217 – 2171N-linked (GlcNAc...)Sequence analysis
Disulfide bondi234 ↔ 249PROSITE-ProRule annotation
Disulfide bondi243 ↔ 260PROSITE-ProRule annotation
Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence analysis
Disulfide bondi262 ↔ 276PROSITE-ProRule annotation
Disulfide bondi282 ↔ 295PROSITE-ProRule annotation
Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence analysis
Disulfide bondi289 ↔ 304PROSITE-ProRule annotation
Disulfide bondi306 ↔ 318PROSITE-ProRule annotation
Modified residuei563 – 5631PhosphothreonineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9LMP1.
PRIDEiQ9LMP1.

Expressioni

Tissue specificityi

Predominantly expressed in green tissues such as stems and leaves. Detected at organ junctions.1 Publication

Developmental stagei

Expressed in shoot and root apical meristems, and in expanding leaves and sepals.1 Publication

Inductioni

Induced by INA and wounding.2 Publications

Gene expression databases

GenevisibleiQ9LMP1. AT.

Interactioni

Protein-protein interaction databases

BioGridi23962. 6 interactions.
STRINGi3702.AT1G21270.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LMP1.
SMRiQ9LMP1. Positions 243-311, 354-724.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini230 – 27748EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini278 – 31942EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini404 – 677274Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IM0I. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiQ9LMP1.
OMAiCFASATK.
PhylomeDBiQ9LMP1.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR025287. WAK_GUB.
[Graphical view]
PfamiPF07645. EGF_CA. 1 hit.
PF13947. GUB_WAK_bind. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LMP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVQEGLFVV AVFYLAYTQL VKGQPRKECQ TRCGNVAVEY PFGTSPGCYY
60 70 80 90 100
PGDESFNLTC NEQEKLFFGN MPVINMSLSG QLRVRLVRSR VCYDSQGKQT
110 120 130 140 150
DYIAQRTTLG NFTLSELNRF TVVGCNSYAF LRTSGVEKYS TGCISICDSA
160 170 180 190 200
TTKNGSCSGE GCCQIPVPRG YSFVRVKPHS FHNHPTVHLF NPCTYAFLVE
210 220 230 240 250
DGMFDFHALE DLNNLRNVTT FPVVLDWSIG DKTCKQVEYR GVCGGNSTCF
260 270 280 290 300
DSTGGTGYNC KCLEGFEGNP YLPNGCQDIN ECISSRHNCS EHSTCENTKG
310 320 330 340 350
SFNCNCPSGY RKDSLNSCTR KVRPEYFRWT QIFLGTTIGF SVIMLGISCL
360 370 380 390 400
QQKIKHRKNT ELRQKFFEQN GGGMLIQRVS GAGPSNVDVK IFTEKGMKEA
410 420 430 440 450
TNGYHESRIL GQGGQGTVYK GILPDNSIVA IKKARLGNRS QVEQFINEVL
460 470 480 490 500
VLSQINHRNV VKVLGCCLET EVPLLVYEFI NSGTLFDHLH GSLYDSSLTW
510 520 530 540 550
EHRLRIATEV AGSLAYLHSS ASIPIIHRDI KTANILLDKN LTAKVADFGA
560 570 580 590 600
SRLIPMDKEQ LTTIVQGTLG YLDPEYYNTG LLNEKSDVYS FGVVLMELLS
610 620 630 640 650
GQKALCFERP HCPKNLVSCF ASATKNNRFH EIIDGQVMNE DNQREIQEAA
660 670 680 690 700
RIAAECTRLM GEERPRMKEV AAELEALRVK TTKYKWSDQY RETGEIEHLL
710 720 730
GVQILSAQGE TSSSIGYDSI RNVTTLDIEA GR
Length:732
Mass (Da):81,644
Last modified:October 1, 2000 - v1
Checksum:i5B1817EF8CA0BEF5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti105 – 1051Q → R (PubMed:10380805).Curated
Sequence conflicti105 – 1051Q → R (Ref. 2) Curated
Sequence conflicti545 – 5451V → A (PubMed:10380805).Curated
Sequence conflicti545 – 5451V → A (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ012423 mRNA. Translation: CAB42872.1.
AF083722 mRNA. Translation: AAN60280.1.
AC036104 Genomic DNA. Translation: AAF81355.1.
CP002684 Genomic DNA. Translation: AEE30080.1.
AY062531 mRNA. Translation: AAL32609.1.
BT010335 mRNA. Translation: AAQ56778.1.
PIRiT52588.
RefSeqiNP_173549.1. NM_101979.3.
UniGeneiAt.21118.
At.23880.
At.69963.

Genome annotation databases

EnsemblPlantsiAT1G21270.1; AT1G21270.1; AT1G21270.
GeneIDi838723.
GrameneiAT1G21270.1; AT1G21270.1; AT1G21270.
KEGGiath:AT1G21270.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ012423 mRNA. Translation: CAB42872.1.
AF083722 mRNA. Translation: AAN60280.1.
AC036104 Genomic DNA. Translation: AAF81355.1.
CP002684 Genomic DNA. Translation: AEE30080.1.
AY062531 mRNA. Translation: AAL32609.1.
BT010335 mRNA. Translation: AAQ56778.1.
PIRiT52588.
RefSeqiNP_173549.1. NM_101979.3.
UniGeneiAt.21118.
At.23880.
At.69963.

3D structure databases

ProteinModelPortaliQ9LMP1.
SMRiQ9LMP1. Positions 243-311, 354-724.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23962. 6 interactions.
STRINGi3702.AT1G21270.1.

Proteomic databases

PaxDbiQ9LMP1.
PRIDEiQ9LMP1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G21270.1; AT1G21270.1; AT1G21270.
GeneIDi838723.
GrameneiAT1G21270.1; AT1G21270.1; AT1G21270.
KEGGiath:AT1G21270.

Organism-specific databases

TAIRiAT1G21270.

Phylogenomic databases

eggNOGiENOG410IM0I. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiQ9LMP1.
OMAiCFASATK.
PhylomeDBiQ9LMP1.

Enzyme and pathway databases

BioCyciARA:AT1G21270-MONOMER.

Miscellaneous databases

PROiQ9LMP1.

Gene expression databases

GenevisibleiQ9LMP1. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR025287. WAK_GUB.
[Graphical view]
PfamiPF07645. EGF_CA. 1 hit.
PF13947. GUB_WAK_bind. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A cluster of five cell wall-associated receptor kinase genes, Wak1-5, are expressed in specific organs of Arabidopsis."
    He Z.-H., Cheeseman I., He D., Kohorn B.D.
    Plant Mol. Biol. 39:1189-1196(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. Columbia.
  2. "Signal peptide selection derived cDNAs from Arabidopsis thaliana leaves and guard cells."
    Stracke R., Palme K.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Wall-associated kinases are expressed throughout plant development and are required for cell expansion."
    Wagner T.A., Kohorn B.D.
    Plant Cell 13:303-318(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, DEVELOPMENTAL STAGE, INTERACTION WITH PECTIN.
  7. "The cell wall-associated kinase (WAK) and WAK-like kinase gene family."
    Verica J.A., He Z.-H.
    Plant Physiol. 129:455-459(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION.

Entry informationi

Entry nameiWAK2_ARATH
AccessioniPrimary (citable) accession number: Q9LMP1
Secondary accession number(s): Q9XGN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.