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Protein

Wall-associated receptor kinase 5

Gene

WAK5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase that may function as a signaling receptor of extracellular matrix component. Binding to pectin may have significance in the control of cell expansion, morphogenesis and development.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei436ATPPROSITE-ProRule annotation1
Active sitei533Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi414 – 422ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wall-associated receptor kinase 5 (EC:2.7.11.-)
Gene namesi
Name:WAK5
Ordered Locus Names:At1g21230
ORF Names:F16F4.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G21230.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 330ExtracellularSequence analysisAdd BLAST307
Transmembranei331 – 351HelicalSequence analysisAdd BLAST21
Topological domaini352 – 733CytoplasmicSequence analysisAdd BLAST382

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000025330424 – 733Wall-associated receptor kinase 5Add BLAST710

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi57N-linked (GlcNAc...)Sequence analysis1
Glycosylationi77N-linked (GlcNAc...)Sequence analysis1
Glycosylationi110N-linked (GlcNAc...)Sequence analysis1
Glycosylationi137N-linked (GlcNAc...)Sequence analysis1
Glycosylationi184N-linked (GlcNAc...)Sequence analysis1
Glycosylationi206N-linked (GlcNAc...)Sequence analysis1
Glycosylationi218N-linked (GlcNAc...)Sequence analysis1
Glycosylationi232N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi235 ↔ 250PROSITE-ProRule annotation
Disulfide bondi244 ↔ 261PROSITE-ProRule annotation
Glycosylationi247N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi263 ↔ 277PROSITE-ProRule annotation
Disulfide bondi283 ↔ 296PROSITE-ProRule annotation
Glycosylationi289N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi290 ↔ 305PROSITE-ProRule annotation
Disulfide bondi307 ↔ 320PROSITE-ProRule annotation
Glycosylationi314N-linked (GlcNAc...)Sequence analysis1
Modified residuei567PhosphothreonineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9LMN7.

Expressioni

Tissue specificityi

Predominantly expressed in green tissues such as stems and leaves.1 Publication

Inductioni

Induced by INA.1 Publication

Gene expression databases

GenevisibleiQ9LMN7. AT.

Interactioni

Protein-protein interaction databases

BioGridi23957. 1 interactor.
IntActiQ9LMN7. 1 interactor.
STRINGi3702.AT1G21230.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LMN7.
SMRiQ9LMN7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini231 – 278EGF-like 1PROSITE-ProRule annotationAdd BLAST48
Domaini279 – 321EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST43
Domaini408 – 691Protein kinasePROSITE-ProRule annotationAdd BLAST284

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi149 – 152Poly-Pro4

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IM0I. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiQ9LMN7.
OMAiTSTCENT.
OrthoDBiEOG0936032S.
PhylomeDBiQ9LMN7.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR025287. WAK_GUB.
[Graphical view]
PfamiPF07645. EGF_CA. 1 hit.
PF13947. GUB_WAK_bind. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LMN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVHSLFLMA IFFYLAYTQL VKAQPRDDCQ TRCGDVPIDY PFGISTGCYY
60 70 80 90 100
PGDDSFNITC EEDKPNVLSN IEVLNFNHSG QLRGLIPRST VCYDQQTNND
110 120 130 140 150
FESLWFRLDN LSFSPNNKFT LVGCNAWALL STFGIQNYST GCMSLCDTPP
160 170 180 190 200
PPNSKCNGVG CCRTEVSIPL DSHRIETQPS RFENMTSVEH FNPCSYAFFV
210 220 230 240 250
EDGMFNFSSL EDLKDLRNVT RFPVLLDWSI GNQTCEQVVG RNICGGNSTC
260 270 280 290 300
FDSTRGKGYN CKCLQGFDGN PYLSDGCQDI NECTTRIHNC SDTSTCENTL
310 320 330 340 350
GSFHCQCPSG SDLNTTTMSC IDTPKEEPKY LGWTTVLLGT TIGFLIILLT
360 370 380 390 400
ISYIQQKMRH RKNTELRQQF FEQNGGGMLI QRLSGAGPSN VDVKIFTEEG
410 420 430 440 450
MKEATDGYNE SRILGQGGQG TVYKGILQDN SIVAIKKARL GDRSQVEQFI
460 470 480 490 500
NEVLVLSQIN HRNVVKLLGC CLETEVPLLV YEFISSGTLF DHLHGSMFDS
510 520 530 540 550
SLTWEHRLRI AIEVAGTLAY LHSYASIPII HRDVKTANIL LDENLTAKVA
560 570 580 590 600
DFGASRLIPM DQEQLTTMVQ GTLGYLDPEY YNTGLLNEKS DVYSFGVVLM
610 620 630 640 650
ELLSGEKALC FERPQSSKHL VSYFVSAMKE NRLHEIIDGQ VMNEYNQREI
660 670 680 690 700
QESARIAVEC TRIMGEERPS MKEVAAELEA LRVKTTKHQW SDQYPKEVEH
710 720 730
LLGVQILSTQ GDTSSIGYDS IQNVTRLDIE TGR
Length:733
Mass (Da):82,206
Last modified:October 1, 2000 - v1
Checksum:i8040BFC89D3D39A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC036104 Genomic DNA. Translation: AAF81359.1.
CP002684 Genomic DNA. Translation: AEE30076.1.
PIRiE86345.
RefSeqiNP_173546.1. NM_101976.2.

Genome annotation databases

EnsemblPlantsiAT1G21230.1; AT1G21230.1; AT1G21230.
GeneIDi838718.
GrameneiAT1G21230.1; AT1G21230.1; AT1G21230.
KEGGiath:AT1G21230.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC036104 Genomic DNA. Translation: AAF81359.1.
CP002684 Genomic DNA. Translation: AEE30076.1.
PIRiE86345.
RefSeqiNP_173546.1. NM_101976.2.

3D structure databases

ProteinModelPortaliQ9LMN7.
SMRiQ9LMN7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23957. 1 interactor.
IntActiQ9LMN7. 1 interactor.
STRINGi3702.AT1G21230.1.

Proteomic databases

PaxDbiQ9LMN7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G21230.1; AT1G21230.1; AT1G21230.
GeneIDi838718.
GrameneiAT1G21230.1; AT1G21230.1; AT1G21230.
KEGGiath:AT1G21230.

Organism-specific databases

TAIRiAT1G21230.

Phylogenomic databases

eggNOGiENOG410IM0I. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiQ9LMN7.
OMAiTSTCENT.
OrthoDBiEOG0936032S.
PhylomeDBiQ9LMN7.

Miscellaneous databases

PROiQ9LMN7.

Gene expression databases

GenevisibleiQ9LMN7. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR025287. WAK_GUB.
[Graphical view]
PfamiPF07645. EGF_CA. 1 hit.
PF13947. GUB_WAK_bind. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWAK5_ARATH
AccessioniPrimary (citable) accession number: Q9LMN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.