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Protein

Wall-associated receptor kinase 5

Gene

WAK5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase that may function as a signaling receptor of extracellular matrix component. Binding to pectin may have significance in the control of cell expansion, morphogenesis and development.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei436 – 4361ATPPROSITE-ProRule annotation
Active sitei533 – 5331Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi414 – 4229ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G21230-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Wall-associated receptor kinase 5 (EC:2.7.11.-)
Gene namesi
Name:WAK5
Ordered Locus Names:At1g21230
ORF Names:F16F4.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G21230.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 330307ExtracellularSequence analysisAdd
BLAST
Transmembranei331 – 35121HelicalSequence analysisAdd
BLAST
Topological domaini352 – 733382CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 733710Wall-associated receptor kinase 5PRO_0000253304Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...)Sequence analysis
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence analysis
Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence analysis
Glycosylationi137 – 1371N-linked (GlcNAc...)Sequence analysis
Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence analysis
Glycosylationi206 – 2061N-linked (GlcNAc...)Sequence analysis
Glycosylationi218 – 2181N-linked (GlcNAc...)Sequence analysis
Glycosylationi232 – 2321N-linked (GlcNAc...)Sequence analysis
Disulfide bondi235 ↔ 250PROSITE-ProRule annotation
Disulfide bondi244 ↔ 261PROSITE-ProRule annotation
Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence analysis
Disulfide bondi263 ↔ 277PROSITE-ProRule annotation
Disulfide bondi283 ↔ 296PROSITE-ProRule annotation
Glycosylationi289 – 2891N-linked (GlcNAc...)Sequence analysis
Disulfide bondi290 ↔ 305PROSITE-ProRule annotation
Disulfide bondi307 ↔ 320PROSITE-ProRule annotation
Glycosylationi314 – 3141N-linked (GlcNAc...)Sequence analysis
Modified residuei567 – 5671PhosphothreonineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9LMN7.
PRIDEiQ9LMN7.

Expressioni

Tissue specificityi

Predominantly expressed in green tissues such as stems and leaves.1 Publication

Inductioni

Induced by INA.1 Publication

Gene expression databases

GenevisibleiQ9LMN7. AT.

Interactioni

Protein-protein interaction databases

BioGridi23957. 1 interaction.
IntActiQ9LMN7. 1 interaction.
STRINGi3702.AT1G21230.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LMN7.
SMRiQ9LMN7. Positions 259-321, 353-693.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini231 – 27848EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini279 – 32143EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini408 – 691284Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi149 – 1524Poly-Pro

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IM0I. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiQ9LMN7.
OMAiTSTCENT.
PhylomeDBiQ9LMN7.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR025287. WAK_GUB.
[Graphical view]
PfamiPF07645. EGF_CA. 1 hit.
PF13947. GUB_WAK_bind. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LMN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVHSLFLMA IFFYLAYTQL VKAQPRDDCQ TRCGDVPIDY PFGISTGCYY
60 70 80 90 100
PGDDSFNITC EEDKPNVLSN IEVLNFNHSG QLRGLIPRST VCYDQQTNND
110 120 130 140 150
FESLWFRLDN LSFSPNNKFT LVGCNAWALL STFGIQNYST GCMSLCDTPP
160 170 180 190 200
PPNSKCNGVG CCRTEVSIPL DSHRIETQPS RFENMTSVEH FNPCSYAFFV
210 220 230 240 250
EDGMFNFSSL EDLKDLRNVT RFPVLLDWSI GNQTCEQVVG RNICGGNSTC
260 270 280 290 300
FDSTRGKGYN CKCLQGFDGN PYLSDGCQDI NECTTRIHNC SDTSTCENTL
310 320 330 340 350
GSFHCQCPSG SDLNTTTMSC IDTPKEEPKY LGWTTVLLGT TIGFLIILLT
360 370 380 390 400
ISYIQQKMRH RKNTELRQQF FEQNGGGMLI QRLSGAGPSN VDVKIFTEEG
410 420 430 440 450
MKEATDGYNE SRILGQGGQG TVYKGILQDN SIVAIKKARL GDRSQVEQFI
460 470 480 490 500
NEVLVLSQIN HRNVVKLLGC CLETEVPLLV YEFISSGTLF DHLHGSMFDS
510 520 530 540 550
SLTWEHRLRI AIEVAGTLAY LHSYASIPII HRDVKTANIL LDENLTAKVA
560 570 580 590 600
DFGASRLIPM DQEQLTTMVQ GTLGYLDPEY YNTGLLNEKS DVYSFGVVLM
610 620 630 640 650
ELLSGEKALC FERPQSSKHL VSYFVSAMKE NRLHEIIDGQ VMNEYNQREI
660 670 680 690 700
QESARIAVEC TRIMGEERPS MKEVAAELEA LRVKTTKHQW SDQYPKEVEH
710 720 730
LLGVQILSTQ GDTSSIGYDS IQNVTRLDIE TGR
Length:733
Mass (Da):82,206
Last modified:October 1, 2000 - v1
Checksum:i8040BFC89D3D39A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC036104 Genomic DNA. Translation: AAF81359.1.
CP002684 Genomic DNA. Translation: AEE30076.1.
PIRiE86345.
RefSeqiNP_173546.1. NM_101976.1.
UniGeneiAt.51703.

Genome annotation databases

EnsemblPlantsiAT1G21230.1; AT1G21230.1; AT1G21230.
GeneIDi838718.
GrameneiAT1G21230.1; AT1G21230.1; AT1G21230.
KEGGiath:AT1G21230.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC036104 Genomic DNA. Translation: AAF81359.1.
CP002684 Genomic DNA. Translation: AEE30076.1.
PIRiE86345.
RefSeqiNP_173546.1. NM_101976.1.
UniGeneiAt.51703.

3D structure databases

ProteinModelPortaliQ9LMN7.
SMRiQ9LMN7. Positions 259-321, 353-693.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23957. 1 interaction.
IntActiQ9LMN7. 1 interaction.
STRINGi3702.AT1G21230.1.

Proteomic databases

PaxDbiQ9LMN7.
PRIDEiQ9LMN7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G21230.1; AT1G21230.1; AT1G21230.
GeneIDi838718.
GrameneiAT1G21230.1; AT1G21230.1; AT1G21230.
KEGGiath:AT1G21230.

Organism-specific databases

TAIRiAT1G21230.

Phylogenomic databases

eggNOGiENOG410IM0I. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiQ9LMN7.
OMAiTSTCENT.
PhylomeDBiQ9LMN7.

Enzyme and pathway databases

BioCyciARA:AT1G21230-MONOMER.

Miscellaneous databases

PROiQ9LMN7.

Gene expression databases

GenevisibleiQ9LMN7. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR025287. WAK_GUB.
[Graphical view]
PfamiPF07645. EGF_CA. 1 hit.
PF13947. GUB_WAK_bind. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "A cluster of five cell wall-associated receptor kinase genes, Wak1-5, are expressed in specific organs of Arabidopsis."
    He Z.-H., Cheeseman I., He D., Kohorn B.D.
    Plant Mol. Biol. 39:1189-1196(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  4. "The cell wall-associated kinase (WAK) and WAK-like kinase gene family."
    Verica J.A., He Z.-H.
    Plant Physiol. 129:455-459(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION.

Entry informationi

Entry nameiWAK5_ARATH
AccessioniPrimary (citable) accession number: Q9LMN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 1, 2000
Last modified: May 11, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.