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Q9LMM5 (MPK11_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 11

Short name=AtMPK11
Short name=MAP kinase 11
EC=2.7.11.24
Gene names
Name:MPK11
Ordered Locus Names:At1g01560
ORF Names:F22L4.10
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length369 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity.

Subunit structure

Interacts with MKK1, MKK2 and MKK6. Ref.6 Ref.7

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-198 and Tyr-200, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9LMM5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9LMM5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     256-275: LIGSPDDSSLGFLRSDNARR → VNFSLFHLTILFRFNLKKEH
     276-369: Missing.
Note: May be due to introns retention. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 369369Mitogen-activated protein kinase 11
PRO_0000245811

Regions

Domain40 – 326287Protein kinase
Nucleotide binding46 – 549ATP By similarity
Motif198 – 2003TXY

Sites

Active site1661Proton acceptor By similarity
Binding site691ATP By similarity

Amino acid modifications

Modified residue1981Phosphothreonine By similarity
Modified residue2001Phosphotyrosine By similarity
Modified residue2031Phosphothreonine By similarity

Natural variations

Alternative sequence256 – 27520LIGSP…DNARR → VNFSLFHLTILFRFNLKKEH in isoform 2.
VSP_035539
Alternative sequence276 – 36994Missing in isoform 2.
VSP_035540

Experimental info

Sequence conflict131N → D in BX815051. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 446673DB42C3DAA6

FASTA36942,475
        10         20         30         40         50         60 
MSIEKPFFGD DSNRGVSING GRYVQYNVYG NLFEVSKKYV PPLRPIGRGA SGIVCAAWNS 

        70         80         90        100        110        120 
ETGEEVAIKK IGNAFGNIID AKRTLREIKL LKHMDHDNVI AIIDIIRPPQ PDNFNDVHIV 

       130        140        150        160        170        180 
YELMDTDLHH IIRSNQPLTD DHSRFFLYQL LRGLKYVHSA NVLHRDLKPS NLLLNANCDL 

       190        200        210        220        230        240 
KIGDFGLART KSETDFMTEY VVTRWYRAPE LLLNCSEYTA AIDIWSVGCI LGEIMTREPL 

       250        260        270        280        290        300 
FPGRDYVQQL RLITELIGSP DDSSLGFLRS DNARRYVRQL PQYPRQNFAA RFPNMSVNAV 

       310        320        330        340        350        360 
DLLQKMLVFD PNRRITVDEA LCHPYLAPLH EYNEEPVCVR PFHFDFEQPS LTEENIKELI 


YRESVKFNP 

« Hide

Isoform 2 [UniParc].

Checksum: B8C8A7A9DCF110C8
Show »

FASTA27531,596

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[4]"Mitogen-activated protein kinase cascades in plants: a new nomenclature."
MAPK group
Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[5]"Ancient signals: comparative genomics of plant MAPK and MAPKK gene families."
Hamel L.P., Nicole M.C., Sritubtim S., Morency M.J., Ellis M., Ehlting J., Beaudoin N., Barbazuk B., Klessig D., Lee J., Martin G., Mundy J., Ohashi Y., Scheel D., Sheen J., Xing T., Zhang S., Seguin A., Ellis B.E.
Trends Plant Sci. 11:192-198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[6]"Comprehensive analysis of protein-protein interactions between Arabidopsis MAPKs and MAPK kinases helps define potential MAPK signalling modules."
Lee J.S., Huh K.W., Bhargava A., Ellis B.E.
Plant Signal. Behav. 3:1037-1041(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MKK1; MKK2 AND MKK6.
[7]"AtMPK4 is required for male-specific meiotic cytokinesis in Arabidopsis."
Zeng Q., Chen J.G., Ellis B.E.
Plant J. 67:895-906(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MKK6.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC061957 Genomic DNA. Translation: AAF81314.1.
CP002684 Genomic DNA. Translation: AEE27305.1.
CP002684 Genomic DNA. Translation: AEE27306.1.
BX815051 mRNA. No translation available.
PIRC86146.
RefSeqNP_001117210.1. NM_001123738.1. [Q9LMM5-1]
NP_563631.2. NM_100038.4. [Q9LMM5-2]
UniGeneAt.49840.

3D structure databases

ProteinModelPortalQ9LMM5.
SMRQ9LMM5. Positions 33-368.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid24758. 1 interaction.
IntActQ9LMM5. 3 interactions.
STRING3702.AT1G01560.2-P.

Proteomic databases

PaxDbQ9LMM5.
PRIDEQ9LMM5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G01560.2; AT1G01560.2; AT1G01560. [Q9LMM5-1]
GeneID839523.
KEGGath:AT1G01560.

Organism-specific databases

GeneFarm845. 89.
TAIRAT1G01560.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
InParanoidQ9LMM5.
KOK04371.
OMAPGRDYVQ.
PhylomeDBQ9LMM5.

Enzyme and pathway databases

BioCycARA:AT1G01560-MONOMER.
ARA:GQT-2439-MONOMER.

Gene expression databases

GenevestigatorQ9LMM5.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK11_ARATH
AccessionPrimary (citable) accession number: Q9LMM5
Secondary accession number(s): B3H762
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names