Reviewed, UniProtKB/Swiss-Prot Q9LMM5 (MPK11_ARATH)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase 11
      Short name=MAP kinase 11
      Short name=AtMPK11
    EC=2.7.11.24

Gene names

Name:	MPK11
Ordered Locus Names:	At1g01560
ORF Names:	F22L4.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by threonine and tyrosine phosphorylation By similarity.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-198 and Tyr-200, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MKK1	Q94A06	1	EBI-2358699,EBI-994464
MKK2	Q9S7U9	1	EBI-2358699,EBI-994350

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9LMM5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9LMM5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     256-275: LIGSPDDSSLGFLRSDNARR → VNFSLFHLTILFRFNLKKEH
     276-369: Missing.

Note: May be due to introns retention. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Mitogen-activated protein kinase 11		PRO_0000245811
Regions
	Domain	40 – 326	287	Protein kinase
	Nucleotide binding	46 – 54	9	ATP By similarity
	Motif	198 – 200	3	TXY
Sites
	Active site	166	1	Proton acceptor By similarity
	Binding site	69	1	ATP By similarity
Amino acid modifications
	Modified residue	198	1	Phosphothreonine By similarity
	Modified residue	200	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	256 – 275	20	LIGSP…DNARR → VNFSLFHLTILFRFNLKKEH in isoform 2.		VSP_035539
	Alternative sequence	276 – 369	94	Missing in isoform 2.		VSP_035540
Experimental info
	Sequence conflict	13	1	N → D in BX815051. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 446673DB42C3DAA6	FASTA	369	42,475
	10 20 30 40 50 60 MSIEKPFFGD DSNRGVSING GRYVQYNVYG NLFEVSKKYV PPLRPIGRGA SGIVCAAWNS 70 80 90 100 110 120 ETGEEVAIKK IGNAFGNIID AKRTLREIKL LKHMDHDNVI AIIDIIRPPQ PDNFNDVHIV 130 140 150 160 170 180 YELMDTDLHH IIRSNQPLTD DHSRFFLYQL LRGLKYVHSA NVLHRDLKPS NLLLNANCDL 190 200 210 220 230 240 KIGDFGLART KSETDFMTEY VVTRWYRAPE LLLNCSEYTA AIDIWSVGCI LGEIMTREPL 250 260 270 280 290 300 FPGRDYVQQL RLITELIGSP DDSSLGFLRS DNARRYVRQL PQYPRQNFAA RFPNMSVNAV 310 320 330 340 350 360 DLLQKMLVFD PNRRITVDEA LCHPYLAPLH EYNEEPVCVR PFHFDFEQPS LTEENIKELI YRESVKFNP
	Isoform 2. Checksum: B8C8A7A9DCF110C8	FASTA	275	31,596
	10 20 30 40 50 60 MSIEKPFFGD DSNRGVSING GRYVQYNVYG NLFEVSKKYV PPLRPIGRGA SGIVCAAWNS 70 80 90 100 110 120 ETGEEVAIKK IGNAFGNIID AKRTLREIKL LKHMDHDNVI AIIDIIRPPQ PDNFNDVHIV 130 140 150 160 170 180 YELMDTDLHH IIRSNQPLTD DHSRFFLYQL LRGLKYVHSA NVLHRDLKPS NLLLNANCDL 190 200 210 220 230 240 KIGDFGLART KSETDFMTEY VVTRWYRAPE LLLNCSEYTA AIDIWSVGCI LGEIMTREPL 250 260 270 FPGRDYVQQL RLITEVNFSL FHLTILFRFN LKKEH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation." Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M. Genome Res. 14:406-413(2004) [PubMed: 14993207] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[3]	"Mitogen-activated protein kinase cascades in plants: a new nomenclature." MAPK group Trends Plant Sci. 7:301-308(2002) [PubMed: 12119167] [Abstract] Cited for: NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC061957 Genomic DNA. Translation: AAF81314.1. BX815051 mRNA. No translation available.
IPI	IPI00545591. IPI00891335.
PIR	C86146.
RefSeq	NP_001117210.1.
UniGene	At.49840
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LMM5. 3 interactions.
Proteomic databases
PRIDE	Q9LMM5.
Genome annotation databases
GeneID	839523.
GenomeReviews	Gene locus AT1G01560 in contig CT485782_GR.
KEGG	ath:AT1G01560.
Organism-specific databases
GeneFarm	845. 89.
TAIR	At1g01560.
Phylogenomic databases
OMA	HENIICI
Enzyme and pathway databases
BRENDA	2.7.11.24. 302.
Gene expression databases
Genevestigator	Q9LMM5.
GermOnline	AT1G01560. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008351. JNK_MAPK. IPR011009. Kinase-like_dom. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01772. JNKMAPKINASE.
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPK11_ARATH

Accession

Primary (citable) accession number: Q9LMM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	October 1, 2000
Last modified:	November 24, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)