Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mitogen-activated protein kinase 11

Gene

MPK11

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69ATPPROSITE-ProRule annotation1
Active sitei166Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 54ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: TAIR

GO - Biological processi

  • response to abscisic acid Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.
R-ATH-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 11 (EC:2.7.11.24)
Short name:
AtMPK11
Short name:
MAP kinase 11
Gene namesi
Name:MPK11
Ordered Locus Names:At1g01560
ORF Names:F22L4.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G01560.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002458111 – 369Mitogen-activated protein kinase 11Add BLAST369

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei198PhosphothreonineBy similarity1
Modified residuei200PhosphotyrosineBy similarity1
Modified residuei203PhosphothreonineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-198 and Tyr-200, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9LMM5.

PTM databases

iPTMnetiQ9LMM5.

Expressioni

Gene expression databases

GenevisibleiQ9LMM5. AT.

Interactioni

Subunit structurei

Interacts with MKK1, MKK2 and MKK6.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MKK1Q94A062EBI-2358699,EBI-994464
MKK2Q9S7U92EBI-2358699,EBI-994350

Protein-protein interaction databases

BioGridi24758. 1 interactor.
IntActiQ9LMM5. 3 interactors.
STRINGi3702.AT1G01560.2.

Structurei

3D structure databases

ProteinModelPortaliQ9LMM5.
SMRiQ9LMM5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 326Protein kinasePROSITE-ProRule annotationAdd BLAST287

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi198 – 200TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ9LMM5.
KOiK04464.
OMAiHIVYELM.
OrthoDBiEOG09360BXQ.
PhylomeDBiQ9LMM5.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LMM5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSIEKPFFGD DSNRGVSING GRYVQYNVYG NLFEVSKKYV PPLRPIGRGA
60 70 80 90 100
SGIVCAAWNS ETGEEVAIKK IGNAFGNIID AKRTLREIKL LKHMDHDNVI
110 120 130 140 150
AIIDIIRPPQ PDNFNDVHIV YELMDTDLHH IIRSNQPLTD DHSRFFLYQL
160 170 180 190 200
LRGLKYVHSA NVLHRDLKPS NLLLNANCDL KIGDFGLART KSETDFMTEY
210 220 230 240 250
VVTRWYRAPE LLLNCSEYTA AIDIWSVGCI LGEIMTREPL FPGRDYVQQL
260 270 280 290 300
RLITELIGSP DDSSLGFLRS DNARRYVRQL PQYPRQNFAA RFPNMSVNAV
310 320 330 340 350
DLLQKMLVFD PNRRITVDEA LCHPYLAPLH EYNEEPVCVR PFHFDFEQPS
360
LTEENIKELI YRESVKFNP
Length:369
Mass (Da):42,475
Last modified:October 1, 2000 - v1
Checksum:i446673DB42C3DAA6
GO
Isoform 2 (identifier: Q9LMM5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     256-275: LIGSPDDSSLGFLRSDNARR → VNFSLFHLTILFRFNLKKEH
     276-369: Missing.

Note: May be due to introns retention. No experimental confirmation available.
Show »
Length:275
Mass (Da):31,596
Checksum:iB8C8A7A9DCF110C8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13N → D in BX815051 (PubMed:14993207).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_035539256 – 275LIGSP…DNARR → VNFSLFHLTILFRFNLKKEH in isoform 2. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_035540276 – 369Missing in isoform 2. 1 PublicationAdd BLAST94

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC061957 Genomic DNA. Translation: AAF81314.1.
CP002684 Genomic DNA. Translation: AEE27305.1.
CP002684 Genomic DNA. Translation: AEE27306.1.
BX815051 mRNA. No translation available.
PIRiC86146.
RefSeqiNP_001117210.1. NM_001123738.2. [Q9LMM5-1]
NP_563631.2. NM_100038.4. [Q9LMM5-2]
UniGeneiAt.49840.

Genome annotation databases

EnsemblPlantsiAT1G01560.2; AT1G01560.2; AT1G01560. [Q9LMM5-1]
GeneIDi839523.
GrameneiAT1G01560.2; AT1G01560.2; AT1G01560.
KEGGiath:AT1G01560.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC061957 Genomic DNA. Translation: AAF81314.1.
CP002684 Genomic DNA. Translation: AEE27305.1.
CP002684 Genomic DNA. Translation: AEE27306.1.
BX815051 mRNA. No translation available.
PIRiC86146.
RefSeqiNP_001117210.1. NM_001123738.2. [Q9LMM5-1]
NP_563631.2. NM_100038.4. [Q9LMM5-2]
UniGeneiAt.49840.

3D structure databases

ProteinModelPortaliQ9LMM5.
SMRiQ9LMM5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24758. 1 interactor.
IntActiQ9LMM5. 3 interactors.
STRINGi3702.AT1G01560.2.

PTM databases

iPTMnetiQ9LMM5.

Proteomic databases

PaxDbiQ9LMM5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G01560.2; AT1G01560.2; AT1G01560. [Q9LMM5-1]
GeneIDi839523.
GrameneiAT1G01560.2; AT1G01560.2; AT1G01560.
KEGGiath:AT1G01560.

Organism-specific databases

TAIRiAT1G01560.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ9LMM5.
KOiK04464.
OMAiHIVYELM.
OrthoDBiEOG09360BXQ.
PhylomeDBiQ9LMM5.

Enzyme and pathway databases

ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ9LMM5.

Gene expression databases

GenevisibleiQ9LMM5. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPK11_ARATH
AccessioniPrimary (citable) accession number: Q9LMM5
Secondary accession number(s): B3H762
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.