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Q9LMM5

- MPK11_ARATH

UniProt

Q9LMM5 - MPK11_ARATH

Protein

Mitogen-activated protein kinase 11

Gene

MPK11

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei69 – 691ATPPROSITE-ProRule annotation
    Active sitei166 – 1661Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi46 – 549ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: TAIR
    3. protein binding Source: IntAct

    GO - Biological processi

    1. response to abscisic acid Source: TAIR

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G01560-MONOMER.
    ARA:GQT-2439-MONOMER.
    ReactomeiREACT_190855. CREB phosphorylation through the activation of Ras.
    REACT_190946. KSRP destabilizes mRNA.
    REACT_202229. ERK1 activation.
    REACT_208246. ERKs are inactivated.
    REACT_209747. ERK2 activation.
    REACT_216613. Signalling to ERK5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 11 (EC:2.7.11.24)
    Short name:
    AtMPK11
    Short name:
    MAP kinase 11
    Gene namesi
    Name:MPK11
    Ordered Locus Names:At1g01560
    ORF Names:F22L4.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G01560.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 369369Mitogen-activated protein kinase 11PRO_0000245811Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei198 – 1981PhosphothreonineBy similarity
    Modified residuei200 – 2001PhosphotyrosineBy similarity
    Modified residuei203 – 2031PhosphothreonineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-198 and Tyr-200, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9LMM5.
    PRIDEiQ9LMM5.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9LMM5.

    Interactioni

    Subunit structurei

    Interacts with MKK1, MKK2 and MKK6.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MKK1Q94A062EBI-2358699,EBI-994464
    MKK2Q9S7U92EBI-2358699,EBI-994350

    Protein-protein interaction databases

    BioGridi24758. 1 interaction.
    IntActiQ9LMM5. 3 interactions.
    STRINGi3702.AT1G01560.2-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LMM5.
    SMRiQ9LMM5. Positions 33-368.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 326287Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi198 – 2003TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    InParanoidiQ9LMM5.
    KOiK04371.
    OMAiPGRDYVQ.
    PhylomeDBiQ9LMM5.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9LMM5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSIEKPFFGD DSNRGVSING GRYVQYNVYG NLFEVSKKYV PPLRPIGRGA    50
    SGIVCAAWNS ETGEEVAIKK IGNAFGNIID AKRTLREIKL LKHMDHDNVI 100
    AIIDIIRPPQ PDNFNDVHIV YELMDTDLHH IIRSNQPLTD DHSRFFLYQL 150
    LRGLKYVHSA NVLHRDLKPS NLLLNANCDL KIGDFGLART KSETDFMTEY 200
    VVTRWYRAPE LLLNCSEYTA AIDIWSVGCI LGEIMTREPL FPGRDYVQQL 250
    RLITELIGSP DDSSLGFLRS DNARRYVRQL PQYPRQNFAA RFPNMSVNAV 300
    DLLQKMLVFD PNRRITVDEA LCHPYLAPLH EYNEEPVCVR PFHFDFEQPS 350
    LTEENIKELI YRESVKFNP 369
    Length:369
    Mass (Da):42,475
    Last modified:October 1, 2000 - v1
    Checksum:i446673DB42C3DAA6
    GO
    Isoform 2 (identifier: Q9LMM5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         256-275: LIGSPDDSSLGFLRSDNARR → VNFSLFHLTILFRFNLKKEH
         276-369: Missing.

    Note: May be due to introns retention. No experimental confirmation available.

    Show »
    Length:275
    Mass (Da):31,596
    Checksum:iB8C8A7A9DCF110C8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131N → D in BX815051. (PubMed:14993207)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei256 – 27520LIGSP…DNARR → VNFSLFHLTILFRFNLKKEH in isoform 2. 1 PublicationVSP_035539Add
    BLAST
    Alternative sequencei276 – 36994Missing in isoform 2. 1 PublicationVSP_035540Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC061957 Genomic DNA. Translation: AAF81314.1.
    CP002684 Genomic DNA. Translation: AEE27305.1.
    CP002684 Genomic DNA. Translation: AEE27306.1.
    BX815051 mRNA. No translation available.
    PIRiC86146.
    RefSeqiNP_001117210.1. NM_001123738.1. [Q9LMM5-1]
    NP_563631.2. NM_100038.4. [Q9LMM5-2]
    UniGeneiAt.49840.

    Genome annotation databases

    EnsemblPlantsiAT1G01560.2; AT1G01560.2; AT1G01560. [Q9LMM5-1]
    GeneIDi839523.
    KEGGiath:AT1G01560.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC061957 Genomic DNA. Translation: AAF81314.1 .
    CP002684 Genomic DNA. Translation: AEE27305.1 .
    CP002684 Genomic DNA. Translation: AEE27306.1 .
    BX815051 mRNA. No translation available.
    PIRi C86146.
    RefSeqi NP_001117210.1. NM_001123738.1. [Q9LMM5-1 ]
    NP_563631.2. NM_100038.4. [Q9LMM5-2 ]
    UniGenei At.49840.

    3D structure databases

    ProteinModelPortali Q9LMM5.
    SMRi Q9LMM5. Positions 33-368.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 24758. 1 interaction.
    IntActi Q9LMM5. 3 interactions.
    STRINGi 3702.AT1G01560.2-P.

    Proteomic databases

    PaxDbi Q9LMM5.
    PRIDEi Q9LMM5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G01560.2 ; AT1G01560.2 ; AT1G01560 . [Q9LMM5-1 ]
    GeneIDi 839523.
    KEGGi ath:AT1G01560.

    Organism-specific databases

    GeneFarmi 845. 89.
    TAIRi AT1G01560.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    InParanoidi Q9LMM5.
    KOi K04371.
    OMAi PGRDYVQ.
    PhylomeDBi Q9LMM5.

    Enzyme and pathway databases

    BioCyci ARA:AT1G01560-MONOMER.
    ARA:GQT-2439-MONOMER.
    Reactomei REACT_190855. CREB phosphorylation through the activation of Ras.
    REACT_190946. KSRP destabilizes mRNA.
    REACT_202229. ERK1 activation.
    REACT_208246. ERKs are inactivated.
    REACT_209747. ERK2 activation.
    REACT_216613. Signalling to ERK5.

    Gene expression databases

    Genevestigatori Q9LMM5.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
      Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
      Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    4. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
      MAPK group
      Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    5. Cited for: GENE FAMILY.
    6. "Comprehensive analysis of protein-protein interactions between Arabidopsis MAPKs and MAPK kinases helps define potential MAPK signalling modules."
      Lee J.S., Huh K.W., Bhargava A., Ellis B.E.
      Plant Signal. Behav. 3:1037-1041(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MKK1; MKK2 AND MKK6.
    7. "AtMPK4 is required for male-specific meiotic cytokinesis in Arabidopsis."
      Zeng Q., Chen J.G., Ellis B.E.
      Plant J. 67:895-906(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MKK6.

    Entry informationi

    Entry nameiMPK11_ARATH
    AccessioniPrimary (citable) accession number: Q9LMM5
    Secondary accession number(s): B3H762
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3