Q9LML6 (U71C4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glycosyltransferase 71C4
EC=2.4.1.-

Alternative name(s):
Flavonol 3-O-glucosyltransferase UGT71C4
EC=2.4.1.91
Flavonol 7-O-glucosyltransferase UGT71C4

Gene names

Name:	UGT71C4
Ordered Locus Names:	At1g07250
ORF Names:	F10K1.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Possesses quercetin 3-O-glucosyltransferase and 7-O-glucosyltransferase activities in vitro. Also active in vitro on benzoates and benzoate derivatives. Ref.5 Ref.6
Catalytic activity	UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside. UDP-glucose + a flavonol = UDP + a flavonol 7-O-D-glucoside.
Sequence similarities	Belongs to the UDP-glycosyltransferase family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	flavonol 3-O-glucosyltransferase activity Inferred from electronic annotation. Source: EC quercetin 3-O-glucosyltransferase activity Inferred from direct assay Ref.6. Source: TAIR quercetin 7-O-glucosyltransferase activity Inferred from direct assay Ref.6. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	UDP-glycosyltransferase 71C4		PRO_0000409056
Regions
	Region	350 – 352	3	UDP-glucose binding By similarity
	Region	367 – 375	9	UDP-glucose binding By similarity
	Region	389 – 392	4	UDP-glucose binding By similarity
Sites
	Binding site	291	1	UDP-glucose By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LML6 [UniParc]. Last modified March 1, 2001. Version 2. Checksum: BEAFE2D5C86F8920	FASTA	479	52,844
	10 20 30 40 50 60 MVKETELIFI PVPSTGHILV HIEFAKRLIN LDHRIHTITI LNLSSPSSPH ASVFARSLIA 70 80 90 100 110 120 SQPKIRLHDL PPIQDPPPFD LYQRAPEAYI VKLIKKNTPL IKDAVSSIVA SRRGGSDSVQ 130 140 150 160 170 180 VAGLVLDLFC NSLVKDVGNE LNLPSYIYLT CNARYLGMMK YIPDRHRKIA SEFDLSSGDE 190 200 210 220 230 240 ELPVPGFINA IPTKFMPPGL FNKEAYEAYV ELAPRFADAK GILVNSFTEL EPHPFDYFSH 250 260 270 280 290 300 LEKFPPVYPV GPILSLKDRA SPNEEAVDRD QIVGWLDDQP ESSVVFLCFG SRGSVDEPQV 310 320 330 340 350 360 KEIARALELV GCRFLWSIRT SGDVETNPND VLPEGFMGRV AGRGLVCGWA PQVEVLAHKA 370 380 390 400 410 420 IGGFVSHCGW NSTLESLWFG VPVATWPMYA EQQLNAFTLV KELGLAVDLR MDYVSSRGGL 430 440 450 460 470 VTCDEIARAV RSLMDGGDEK RKKVKEMADA ARKALMDGGS SSLATARFIA ELFEDGSSC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana." Li Y., Baldauf S., Lim E.K., Bowles D.J. J. Biol. Chem. 276:4338-4343(2001) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[5]	"The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates." Lim E.K., Doucet C.J., Li Y., Elias L., Worrall D., Spencer S.P., Ross J., Bowles D.J. J. Biol. Chem. 277:586-592(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides." Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J. Biotechnol. Bioeng. 87:623-631(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC067971 Genomic DNA. Translation: AAG18592.1. CP002684 Genomic DNA. Translation: AEE28097.1. AY040019 mRNA. Translation: AAK64176.2. BT001938 mRNA. Translation: AAN71937.1.
IPI	IPI00521753.
PIR	G86207.
RefSeq	NP_563784.2. NM_100599.3.
UniGene	At.17149.
3D structure databases
ProteinModelPortal	Q9LML6.
SMR	Q9LML6. Positions 3-472.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G07250.1-P.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Proteomic databases
PaxDb	Q9LML6.
PRIDE	Q9LML6.
Protocols and materials databases
DNASU	837236.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G07250.1; AT1G07250.1; AT1G07250.
GeneID	837236.
KEGG	ath:AT1G07250.
Organism-specific databases
TAIR	At1g07250.
Phylogenomic databases
eggNOG	NOG265229.
HOGENOM	HOG000237568.
InParanoid	Q9LML6.
OMA	KETELIF.
ProtClustDB	PLN02167.
Gene expression databases
Genevestigator	Q9LML6.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. PTHR11926. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

U71C4_ARATH

Accession

Primary (citable) accession number: Q9LML6
Secondary accession number(s): Q94BM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program