Q9LMI0 (TPS7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 7
EC=2.4.1.15

Alternative name(s):
Trehalose-6-phosphate synthase 7
Short name=AtTPS7

Gene names

Name:	TPS7
Synonyms:	TPSA
Ordered Locus Names:	At1g06410
ORF Names:	T2D23.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	851 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.
Subunit structure	Binds to the phosphopeptide-binding site of GRF/14-3-3. Ref.6
Tissue specificity	Expressed in seedlings, leaves, roots, stems, flowers and siliques. Ref.1
Post-translational modification	Phosphorylated. Ref.6
Sequence similarities	In the N-terminal section; belongs to the glycosyltransferase 20 family. In the C-terminal section; belongs to the trehalose phosphatase family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Inferred from direct assay PubMed 21166475. Source: TAIR
Molecular_function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 851	851	Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 7		PRO_0000324828
Regions
	Region	59 – 540	482	Glycosyltransferase

Sequences

	Sequence		Length	Mass (Da)
	Q9LMI0 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 1799F009CF8E4A1B	FASTA	851	96,688
	10 20 30 40 50 60 MISRSYTNLL DLASGNFPVM GRERRRLPRV MTVPGNVSEF DEDQAYSVSS DNPSSVSSDR 70 80 90 100 110 120 MIIVANRLPL KAEKRNGSWS FSWDQDSLYL QLKDGLPEDM EILYVGSLSV DVDSNEQDDV 130 140 150 160 170 180 AQILLDKFKC VPTFFPPDLQ SKFYDGFCKR QIWPLFHYML PFSADHGGRF DRSLWEAYVA 190 200 210 220 230 240 TNKLFFQKVI EVINPDDDFV WIHDYHLMVL PTFLRRRFNR IRMGFFLHSP FPSSEIYRSL 250 260 270 280 290 300 PVREEILKAL LNSDLIGFHT FDYARHFLTC CSRMLGLEYQ SKRGYIGLEY YGRTVGIKIM 310 320 330 340 350 360 PVGINMGRIQ SVMRYSEEEG KVMELRNRFE GKTVLLGIDD MDIFKGINLK LLAMEQMLRQ 370 380 390 400 410 420 HPNWRGRAVL VQIVNPARGK GIDVEEIRGE IEESCRRING EFGKPGYQPI IYIDTPVSIN 430 440 450 460 470 480 EINAYYHIAE CVVVTAVRDG MNLTPYEYIV CRQGLLGSES DFSGPKKSML VASEFIGCSP 490 500 510 520 530 540 SLSGAIRVNP WNVEATGEAL NEALSMSDAE KQLRHEKHFR YVSTHDVAYW SRSFLQDLER 550 560 570 580 590 600 ICVDHFKKRC WGMGISFGFR VVALDPNFRK LSIPCIVSDY KRAKSRAILL DYDGTLMPQN 610 620 630 640 650 660 SINKAPSQEV LNFLDALCED KKNSIFIVSG RGRESLSKWF TPCKKIGIAA EHGYFLKWSG 670 680 690 700 710 720 SEEWETCGQS SDFGWMQIVE PVMKQYTEST DGSSIEIKES ALVWQYRDAD PGFGSLQAKE 730 740 750 760 770 780 MLEHLESVLA NEPVAVKSGH YIVEVKPQGV SKGSVSEKIF SSMAGKGKPV DFVLCIGDDR 790 800 810 820 830 840 SDEDMFEAIG NAMSKRLLCD NALVFACTVG QKPSKAKYYL DDTTEVTCML ESLAEASEAS 850 NFSMRELDEA L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Trehalose metabolism in Arabidopsis: occurrence of trehalose and molecular cloning and characterization of trehalose-6-phosphate synthase homologues." Vogel G., Fiehn O., Jean-Richard-dit-Bressel L., Boller T., Wiemken A., Aeschbacher R.A., Wingler A. J. Exp. Bot. 52:1817-1826(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: cv. Landsberg erecta.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 559-851. Strain: cv. Columbia.
[5]	"An unexpected plethora of trehalose biosynthesis genes in Arabidopsis thaliana." Leyman B., Van Dijck P., Thevelein J.M. Trends Plant Sci. 6:510-513(2001) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Phosphorylation and 14-3-3 binding of Arabidopsis trehalose-phosphate synthase 5 in response to 2-deoxyglucose." Harthill J.E., Meek S.E.M., Morrice N., Peggie M.W., Borch J., Wong B.H.C., Mackintosh C. Plant J. 47:211-223(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION, INTERACTION WITH GRF/14-3-3, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC068143 Genomic DNA. Translation: AAF82169.1. CP002684 Genomic DNA. Translation: AEE27983.1. AK228947 mRNA. Translation: BAF00836.1.
IPI	IPI00530498.
PIR	A86200.
RefSeq	NP_172129.1. NM_100521.2.
UniGene	At.43841.
3D structure databases
HSSP	HSSP built from PDB template 1GZ5 based on UniProtKB P31677.
ProteinModelPortal	Q9LMI0.
SMR	Q9LMI0. Positions 142-541, 588-831.
ModBase	Search...
Protein family/group databases
CAZy	GT20. Glycosyltransferase Family 20.
Proteomic databases
PaxDb	Q9LMI0.
PRIDE	Q9LMI0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G06410.1; AT1G06410.1; AT1G06410.
GeneID	837152.
KEGG	ath:AT1G06410.
Organism-specific databases
TAIR	At1g06410.
Phylogenomic databases
eggNOG	COG0380.
HOGENOM	HOG000191476.
InParanoid	Q9LMI0.
KO	K16055.
OMA	IIVANRL.
PhylomeDB	Q9LMI0.
ProtClustDB	CLSN2682317.
Gene expression databases
Genevestigator	Q9LMI0.
Family and domain databases
Gene3D	3.40.50.1000. 2 hits.
InterPro	IPR001830. Glyco_trans_20. IPR023214. HAD-like_dom. IPR006379. HAD-SF_hydro_IIB. IPR003337. Trehalose_PPase. [Graphical view]
Pfam	PF00982. Glyco_transf_20. 1 hit. PF02358. Trehalose_PPase. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01484. HAD-SF-IIB. 1 hit. TIGR00685. T6PP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPS7_ARATH

Accession

Primary (citable) accession number: Q9LMI0
Secondary accession number(s): Q0WPW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program