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Q9LMI0 (TPS7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 7

EC=2.4.1.15
Alternative name(s):
Trehalose-6-phosphate synthase 7
Short name=AtTPS7
Gene names
Name:TPS7
Synonyms:TPSA
Ordered Locus Names:At1g06410
ORF Names:T2D23.11
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length851 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Subunit structure

Binds to the phosphopeptide-binding site of GRF/14-3-3. Ref.6

Tissue specificity

Expressed in seedlings, leaves, roots, stems, flowers and siliques. Ref.1

Post-translational modification

Phosphorylated. Ref.6

Sequence similarities

In the N-terminal section; belongs to the glycosyltransferase 20 family.

In the C-terminal section; belongs to the trehalose phosphatase family.

Ontologies

Keywords
   Molecular functionGlycosyltransferase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtrehalose biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytosol

Inferred from direct assay PubMed 21166475. Source: TAIR

   Molecular_functiontransferase activity, transferring glycosyl groups

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 851851Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 7
PRO_0000324828

Regions

Region59 – 540482Glycosyltransferase

Amino acid modifications

Modified residue51Phosphoserine By similarity
Modified residue321Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9LMI0 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 1799F009CF8E4A1B

FASTA85196,688
        10         20         30         40         50         60 
MISRSYTNLL DLASGNFPVM GRERRRLPRV MTVPGNVSEF DEDQAYSVSS DNPSSVSSDR 

        70         80         90        100        110        120 
MIIVANRLPL KAEKRNGSWS FSWDQDSLYL QLKDGLPEDM EILYVGSLSV DVDSNEQDDV 

       130        140        150        160        170        180 
AQILLDKFKC VPTFFPPDLQ SKFYDGFCKR QIWPLFHYML PFSADHGGRF DRSLWEAYVA 

       190        200        210        220        230        240 
TNKLFFQKVI EVINPDDDFV WIHDYHLMVL PTFLRRRFNR IRMGFFLHSP FPSSEIYRSL 

       250        260        270        280        290        300 
PVREEILKAL LNSDLIGFHT FDYARHFLTC CSRMLGLEYQ SKRGYIGLEY YGRTVGIKIM 

       310        320        330        340        350        360 
PVGINMGRIQ SVMRYSEEEG KVMELRNRFE GKTVLLGIDD MDIFKGINLK LLAMEQMLRQ 

       370        380        390        400        410        420 
HPNWRGRAVL VQIVNPARGK GIDVEEIRGE IEESCRRING EFGKPGYQPI IYIDTPVSIN 

       430        440        450        460        470        480 
EINAYYHIAE CVVVTAVRDG MNLTPYEYIV CRQGLLGSES DFSGPKKSML VASEFIGCSP 

       490        500        510        520        530        540 
SLSGAIRVNP WNVEATGEAL NEALSMSDAE KQLRHEKHFR YVSTHDVAYW SRSFLQDLER 

       550        560        570        580        590        600 
ICVDHFKKRC WGMGISFGFR VVALDPNFRK LSIPCIVSDY KRAKSRAILL DYDGTLMPQN 

       610        620        630        640        650        660 
SINKAPSQEV LNFLDALCED KKNSIFIVSG RGRESLSKWF TPCKKIGIAA EHGYFLKWSG 

       670        680        690        700        710        720 
SEEWETCGQS SDFGWMQIVE PVMKQYTEST DGSSIEIKES ALVWQYRDAD PGFGSLQAKE 

       730        740        750        760        770        780 
MLEHLESVLA NEPVAVKSGH YIVEVKPQGV SKGSVSEKIF SSMAGKGKPV DFVLCIGDDR 

       790        800        810        820        830        840 
SDEDMFEAIG NAMSKRLLCD NALVFACTVG QKPSKAKYYL DDTTEVTCML ESLAEASEAS 

       850 
NFSMRELDEA L 

« Hide

References

« Hide 'large scale' references
[1]"Trehalose metabolism in Arabidopsis: occurrence of trehalose and molecular cloning and characterization of trehalose-6-phosphate synthase homologues."
Vogel G., Fiehn O., Jean-Richard-dit-Bressel L., Boller T., Wiemken A., Aeschbacher R.A., Wingler A.
J. Exp. Bot. 52:1817-1826(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: cv. Landsberg erecta.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 559-851.
Strain: cv. Columbia.
[5]"An unexpected plethora of trehalose biosynthesis genes in Arabidopsis thaliana."
Leyman B., Van Dijck P., Thevelein J.M.
Trends Plant Sci. 6:510-513(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[6]"Phosphorylation and 14-3-3 binding of Arabidopsis trehalose-phosphate synthase 5 in response to 2-deoxyglucose."
Harthill J.E., Meek S.E.M., Morrice N., Peggie M.W., Borch J., Wong B.H.C., Mackintosh C.
Plant J. 47:211-223(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION, INTERACTION WITH GRF/14-3-3, IDENTIFICATION BY MASS SPECTROMETRY.
[7]"Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.
[8]"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC068143 Genomic DNA. Translation: AAF82169.1.
CP002684 Genomic DNA. Translation: AEE27983.1.
AK228947 mRNA. Translation: BAF00836.1.
PIRA86200.
RefSeqNP_172129.1. NM_100521.2.
UniGeneAt.43841.

3D structure databases

ProteinModelPortalQ9LMI0.
SMRQ9LMI0. Positions 59-542, 588-831.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid22393. 1 interaction.

Protein family/group databases

CAZyGT20. Glycosyltransferase Family 20.

Proteomic databases

PaxDbQ9LMI0.
PRIDEQ9LMI0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G06410.1; AT1G06410.1; AT1G06410.
GeneID837152.
KEGGath:AT1G06410.

Organism-specific databases

TAIRAT1G06410.

Phylogenomic databases

eggNOGCOG0380.
HOGENOMHOG000191476.
InParanoidQ9LMI0.
KOK16055.
OMAVPDEMEV.
PhylomeDBQ9LMI0.
ProtClustDBCLSN2682317.

Enzyme and pathway databases

BioCycARA:AT1G06410-MONOMER.

Gene expression databases

GenevestigatorQ9LMI0.

Family and domain databases

Gene3D3.40.50.1000. 2 hits.
InterProIPR001830. Glyco_trans_20.
IPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR003337. Trehalose_PPase.
[Graphical view]
PfamPF00982. Glyco_transf_20. 1 hit.
PF02358. Trehalose_PPase. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 1 hit.
TIGRFAMsTIGR01484. HAD-SF-IIB. 1 hit.
TIGR00685. T6PP. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTPS7_ARATH
AccessionPrimary (citable) accession number: Q9LMI0
Secondary accession number(s): Q0WPW3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names