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Q9LME4

- P2C09_ARATH

UniProt

Q9LME4 - P2C09_ARATH

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Protein

Probable protein phosphatase 2C 9

Gene

At1g22280

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the regulation of phytochrome signaling. May regulate phytochrome-interacting factor 3 (PIF3) through the dephosphorylation of phytochrome.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Manganese 1By similarity
Metal bindingi70 – 701Manganese 2By similarity
Metal bindingi71 – 711Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi232 – 2321Manganese 2By similarity
Metal bindingi271 – 2711Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G22280-MONOMER.
ARA:GQT-527-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 9 (EC:3.1.3.16)
Short name:
AtPP2C09
Alternative name(s):
Phytochrome-associated protein phosphatase 2C
Short name:
PAPP2C
Gene namesi
Ordered Locus Names:At1g22280
ORF Names:T16E15.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G22280.

Subcellular locationi

Nucleus 2 Publications
Note: Localizes in the nucleus upon illumination.

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Abnormal hypocotyl elongation under continuous red light.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 281281Probable protein phosphatase 2C 9PRO_0000367940Add
BLAST

Proteomic databases

PaxDbiQ9LME4.
PRIDEiQ9LME4.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LME4. baseline and differential.
GenevestigatoriQ9LME4.

Interactioni

Subunit structurei

Interacts with phytochromes (via N-terminus).1 Publication

Protein-protein interaction databases

BioGridi24074. 2 interactions.
IntActiQ9LME4. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9LME4.
SMRiQ9LME4. Positions 65-281.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 273242PP2C-likeAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PP2C-like domain.Curated

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000233896.
InParanoidiQ9LME4.
PhylomeDBiQ9LME4.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9LME4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKFCCFTSA SEVVGGQSSS RSGKGRSDEG MIKYGFSLVK GKANHPMEDY
60 70 80 90 100
HVANFINIQD HELGLFAIYD GHMGDSVPAY LQKRLFSNIL KEGEFWVDPR
110 120 130 140 150
RSIAKAYEKT DQAILSNSSD LGRGGSTAVT AILINGRKLW IANVGDSRAV
160 170 180 190 200
LSHGGAITQM STDHEPRTER SSIEDRGGFV SNLPGDVPRV NGQLAVSRAF
210 220 230 240 250
GDKGLKTHLS SEPDIKEATV DSQTDVLLLA SDGIWKVMTN EEAMEIARRV
260 270 280
KDPQKAAKEL TAEALRRESK DDISCVVVRF R
Length:281
Mass (Da):30,722
Last modified:October 1, 2000 - v1
Checksum:i2EE90E4F8FB56768
GO
Isoform 2 (identifier: Q9LME4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     186-199: DVPRVNGQLAVSRA → KELILSIECLMKIE
     200-281: Missing.

Note: No experimental confirmation available.

Show »
Length:199
Mass (Da):21,732
Checksum:i502ABB9152C7E9B2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti111 – 1122DQ → E in AAM61437. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei186 – 19914DVPRV…AVSRA → KELILSIECLMKIE in isoform 2. 1 PublicationVSP_036759Add
BLAST
Alternative sequencei200 – 28182Missing in isoform 2. 1 PublicationVSP_036760Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC068562 Genomic DNA. Translation: AAF87263.1.
CP002684 Genomic DNA. Translation: AEE30220.1.
CP002684 Genomic DNA. Translation: AEE30221.1.
AY080735 mRNA. Translation: AAL86005.1.
AY133737 mRNA. Translation: AAM91671.1.
BX813947 mRNA. No translation available.
AY084874 mRNA. Translation: AAM61437.1.
PIRiF86355.
RefSeqiNP_564165.1. NM_102079.4. [Q9LME4-1]
NP_973883.1. NM_202154.2. [Q9LME4-2]
UniGeneiAt.41611.

Genome annotation databases

EnsemblPlantsiAT1G22280.1; AT1G22280.1; AT1G22280. [Q9LME4-1]
GeneIDi838835.
KEGGiath:AT1G22280.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC068562 Genomic DNA. Translation: AAF87263.1 .
CP002684 Genomic DNA. Translation: AEE30220.1 .
CP002684 Genomic DNA. Translation: AEE30221.1 .
AY080735 mRNA. Translation: AAL86005.1 .
AY133737 mRNA. Translation: AAM91671.1 .
BX813947 mRNA. No translation available.
AY084874 mRNA. Translation: AAM61437.1 .
PIRi F86355.
RefSeqi NP_564165.1. NM_102079.4. [Q9LME4-1 ]
NP_973883.1. NM_202154.2. [Q9LME4-2 ]
UniGenei At.41611.

3D structure databases

ProteinModelPortali Q9LME4.
SMRi Q9LME4. Positions 65-281.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 24074. 2 interactions.
IntActi Q9LME4. 2 interactions.

Proteomic databases

PaxDbi Q9LME4.
PRIDEi Q9LME4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G22280.1 ; AT1G22280.1 ; AT1G22280 . [Q9LME4-1 ]
GeneIDi 838835.
KEGGi ath:AT1G22280.

Organism-specific databases

TAIRi AT1G22280.

Phylogenomic databases

eggNOGi COG0631.
HOGENOMi HOG000233896.
InParanoidi Q9LME4.
PhylomeDBi Q9LME4.

Enzyme and pathway databases

BioCyci ARA:AT1G22280-MONOMER.
ARA:GQT-527-MONOMER.

Gene expression databases

ExpressionAtlasi Q9LME4. baseline and differential.
Genevestigatori Q9LME4.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
    Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
    Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "Identification of phytochrome-interacting protein candidates in Arabidopsis thaliana by co-immunoprecipitation coupled with MALDI-TOF MS."
    Phee B.-K., Shin D.H., Cho J.-H., Kim S.-H., Kim J.-I., Lee Y.-H., Jeon J.-S., Bhoo S.H., Hahn T.-R.
    Proteomics 6:3671-3680(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, INTERACTION WITH PHYTOCHROMES, IDENTIFICATION BY MASS SPECTROMETRY.
  7. "Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis."
    Niittylae T., Fuglsang A.T., Palmgren M.G., Frommer W.B., Schulze W.X.
    Mol. Cell. Proteomics 6:1711-1726(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Seedling.
  8. "A novel protein phosphatase indirectly regulates phytochrome-interacting factor 3 via phytochrome."
    Phee B.-K., Kim J.-I., Shin D.H., Yoo J., Park K.-J., Han Y.-J., Kwon Y.-K., Cho M.H., Jeon J.-S., Bhoo S.H., Hahn T.-R.
    Biochem. J. 415:247-255(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  9. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Root.
  11. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  12. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiP2C09_ARATH
AccessioniPrimary (citable) accession number: Q9LME4
Secondary accession number(s): Q3ED74, Q8LFF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3