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Protein

Probable protein phosphatase 2C 9

Gene

At1g22280

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of phytochrome signaling. May regulate phytochrome-interacting factor 3 (PIF3) through the dephosphorylation of phytochrome.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Manganese 1By similarity
Metal bindingi70 – 701Manganese 2By similarity
Metal bindingi71 – 711Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi232 – 2321Manganese 2By similarity
Metal bindingi271 – 2711Manganese 2By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G22280-MONOMER.
ARA:GQT-527-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 9 (EC:3.1.3.16)
Short name:
AtPP2C09
Alternative name(s):
Phytochrome-associated protein phosphatase 2C
Short name:
PAPP2C
Gene namesi
Ordered Locus Names:At1g22280
ORF Names:T16E15.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G22280.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Abnormal hypocotyl elongation under continuous red light.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 281281Probable protein phosphatase 2C 9PRO_0000367940Add
BLAST

Proteomic databases

PaxDbiQ9LME4.
PRIDEiQ9LME4.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LME4. baseline and differential.
GenevestigatoriQ9LME4.

Interactioni

Subunit structurei

Interacts with phytochromes (via N-terminus).1 Publication

Protein-protein interaction databases

BioGridi24074. 2 interactions.
IntActiQ9LME4. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9LME4.
SMRiQ9LME4. Positions 34-281.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 280248PPM-type phosphatasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000233896.
InParanoidiQ9LME4.
PhylomeDBiQ9LME4.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS51746. PPM_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LME4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKFCCFTSA SEVVGGQSSS RSGKGRSDEG MIKYGFSLVK GKANHPMEDY
60 70 80 90 100
HVANFINIQD HELGLFAIYD GHMGDSVPAY LQKRLFSNIL KEGEFWVDPR
110 120 130 140 150
RSIAKAYEKT DQAILSNSSD LGRGGSTAVT AILINGRKLW IANVGDSRAV
160 170 180 190 200
LSHGGAITQM STDHEPRTER SSIEDRGGFV SNLPGDVPRV NGQLAVSRAF
210 220 230 240 250
GDKGLKTHLS SEPDIKEATV DSQTDVLLLA SDGIWKVMTN EEAMEIARRV
260 270 280
KDPQKAAKEL TAEALRRESK DDISCVVVRF R
Length:281
Mass (Da):30,722
Last modified:October 1, 2000 - v1
Checksum:i2EE90E4F8FB56768
GO
Isoform 2 (identifier: Q9LME4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     186-199: DVPRVNGQLAVSRA → KELILSIECLMKIE
     200-281: Missing.

Note: No experimental confirmation available.

Show »
Length:199
Mass (Da):21,732
Checksum:i502ABB9152C7E9B2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti111 – 1122DQ → E in AAM61437 (Ref. 5) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei186 – 19914DVPRV…AVSRA → KELILSIECLMKIE in isoform 2. 1 PublicationVSP_036759Add
BLAST
Alternative sequencei200 – 28182Missing in isoform 2. 1 PublicationVSP_036760Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC068562 Genomic DNA. Translation: AAF87263.1.
CP002684 Genomic DNA. Translation: AEE30220.1.
CP002684 Genomic DNA. Translation: AEE30221.1.
AY080735 mRNA. Translation: AAL86005.1.
AY133737 mRNA. Translation: AAM91671.1.
BX813947 mRNA. No translation available.
AY084874 mRNA. Translation: AAM61437.1.
PIRiF86355.
RefSeqiNP_564165.1. NM_102079.4. [Q9LME4-1]
NP_973883.1. NM_202154.2. [Q9LME4-2]
UniGeneiAt.41611.

Genome annotation databases

EnsemblPlantsiAT1G22280.1; AT1G22280.1; AT1G22280. [Q9LME4-1]
GeneIDi838835.
KEGGiath:AT1G22280.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC068562 Genomic DNA. Translation: AAF87263.1.
CP002684 Genomic DNA. Translation: AEE30220.1.
CP002684 Genomic DNA. Translation: AEE30221.1.
AY080735 mRNA. Translation: AAL86005.1.
AY133737 mRNA. Translation: AAM91671.1.
BX813947 mRNA. No translation available.
AY084874 mRNA. Translation: AAM61437.1.
PIRiF86355.
RefSeqiNP_564165.1. NM_102079.4. [Q9LME4-1]
NP_973883.1. NM_202154.2. [Q9LME4-2]
UniGeneiAt.41611.

3D structure databases

ProteinModelPortaliQ9LME4.
SMRiQ9LME4. Positions 34-281.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24074. 2 interactions.
IntActiQ9LME4. 2 interactions.

Proteomic databases

PaxDbiQ9LME4.
PRIDEiQ9LME4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G22280.1; AT1G22280.1; AT1G22280. [Q9LME4-1]
GeneIDi838835.
KEGGiath:AT1G22280.

Organism-specific databases

TAIRiAT1G22280.

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000233896.
InParanoidiQ9LME4.
PhylomeDBiQ9LME4.

Enzyme and pathway databases

BioCyciARA:AT1G22280-MONOMER.
ARA:GQT-527-MONOMER.

Miscellaneous databases

PROiQ9LME4.

Gene expression databases

ExpressionAtlasiQ9LME4. baseline and differential.
GenevestigatoriQ9LME4.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
    Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
    Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "Identification of phytochrome-interacting protein candidates in Arabidopsis thaliana by co-immunoprecipitation coupled with MALDI-TOF MS."
    Phee B.-K., Shin D.H., Cho J.-H., Kim S.-H., Kim J.-I., Lee Y.-H., Jeon J.-S., Bhoo S.H., Hahn T.-R.
    Proteomics 6:3671-3680(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, INTERACTION WITH PHYTOCHROMES, IDENTIFICATION BY MASS SPECTROMETRY.
  7. "Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis."
    Niittylae T., Fuglsang A.T., Palmgren M.G., Frommer W.B., Schulze W.X.
    Mol. Cell. Proteomics 6:1711-1726(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Seedling.
  8. "A novel protein phosphatase indirectly regulates phytochrome-interacting factor 3 via phytochrome."
    Phee B.-K., Kim J.-I., Shin D.H., Yoo J., Park K.-J., Han Y.-J., Kwon Y.-K., Cho M.H., Jeon J.-S., Bhoo S.H., Hahn T.-R.
    Biochem. J. 415:247-255(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  9. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Root.
  11. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  12. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiP2C09_ARATH
AccessioniPrimary (citable) accession number: Q9LME4
Secondary accession number(s): Q3ED74, Q8LFF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 1, 2000
Last modified: May 27, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.