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Q9LME4

- P2C09_ARATH

UniProt

Q9LME4 - P2C09_ARATH

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Protein
Probable protein phosphatase 2C 9
Gene
At1g22280, T16E15.10
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the regulation of phytochrome signaling. May regulate phytochrome-interacting factor 3 (PIF3) through the dephosphorylation of phytochrome.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Manganese 1 By similarity
Metal bindingi70 – 701Manganese 2 By similarity
Metal bindingi71 – 711Manganese 1; via carbonyl oxygen By similarity
Metal bindingi232 – 2321Manganese 2 By similarity
Metal bindingi271 – 2711Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G22280-MONOMER.
    ARA:GQT-527-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable protein phosphatase 2C 9 (EC:3.1.3.16)
    Short name:
    AtPP2C09
    Alternative name(s):
    Phytochrome-associated protein phosphatase 2C
    Short name:
    PAPP2C
    Gene namesi
    Ordered Locus Names:At1g22280
    ORF Names:T16E15.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G22280.

    Subcellular locationi

    Nucleus
    Note: Localizes in the nucleus upon illumination.2 Publications

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Abnormal hypocotyl elongation under continuous red light.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 281281Probable protein phosphatase 2C 9
    PRO_0000367940Add
    BLAST

    Proteomic databases

    PaxDbiQ9LME4.
    PRIDEiQ9LME4.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9LME4.
    GenevestigatoriQ9LME4.

    Interactioni

    Subunit structurei

    Interacts with phytochromes (via N-terminus).1 Publication

    Protein-protein interaction databases

    BioGridi24074. 2 interactions.
    IntActiQ9LME4. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LME4.
    SMRiQ9LME4. Positions 34-281.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini32 – 273242PP2C-like
    Add
    BLAST

    Sequence similaritiesi

    Belongs to the PP2C family.
    Contains 1 PP2C-like domain.

    Phylogenomic databases

    eggNOGiCOG0631.
    HOGENOMiHOG000233896.
    InParanoidiQ9LME4.
    PhylomeDBiQ9LME4.

    Family and domain databases

    Gene3Di3.60.40.10. 1 hit.
    InterProiIPR001932. PP2C-like_dom.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9LME4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGKFCCFTSA SEVVGGQSSS RSGKGRSDEG MIKYGFSLVK GKANHPMEDY    50
    HVANFINIQD HELGLFAIYD GHMGDSVPAY LQKRLFSNIL KEGEFWVDPR 100
    RSIAKAYEKT DQAILSNSSD LGRGGSTAVT AILINGRKLW IANVGDSRAV 150
    LSHGGAITQM STDHEPRTER SSIEDRGGFV SNLPGDVPRV NGQLAVSRAF 200
    GDKGLKTHLS SEPDIKEATV DSQTDVLLLA SDGIWKVMTN EEAMEIARRV 250
    KDPQKAAKEL TAEALRRESK DDISCVVVRF R 281
    Length:281
    Mass (Da):30,722
    Last modified:October 1, 2000 - v1
    Checksum:i2EE90E4F8FB56768
    GO
    Isoform 2 (identifier: Q9LME4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         186-199: DVPRVNGQLAVSRA → KELILSIECLMKIE
         200-281: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:199
    Mass (Da):21,732
    Checksum:i502ABB9152C7E9B2
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei186 – 19914DVPRV…AVSRA → KELILSIECLMKIE in isoform 2.
    VSP_036759Add
    BLAST
    Alternative sequencei200 – 28182Missing in isoform 2.
    VSP_036760Add
    BLAST

    Sequence conflict

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti111 – 1122DQ → E in AAM61437. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC068562 Genomic DNA. Translation: AAF87263.1.
    CP002684 Genomic DNA. Translation: AEE30220.1.
    CP002684 Genomic DNA. Translation: AEE30221.1.
    AY080735 mRNA. Translation: AAL86005.1.
    AY133737 mRNA. Translation: AAM91671.1.
    BX813947 mRNA. No translation available.
    AY084874 mRNA. Translation: AAM61437.1.
    PIRiF86355.
    RefSeqiNP_564165.1. NM_102079.4. [Q9LME4-1]
    NP_973883.1. NM_202154.2. [Q9LME4-2]
    UniGeneiAt.41611.

    Genome annotation databases

    EnsemblPlantsiAT1G22280.1; AT1G22280.1; AT1G22280. [Q9LME4-1]
    GeneIDi838835.
    KEGGiath:AT1G22280.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC068562 Genomic DNA. Translation: AAF87263.1 .
    CP002684 Genomic DNA. Translation: AEE30220.1 .
    CP002684 Genomic DNA. Translation: AEE30221.1 .
    AY080735 mRNA. Translation: AAL86005.1 .
    AY133737 mRNA. Translation: AAM91671.1 .
    BX813947 mRNA. No translation available.
    AY084874 mRNA. Translation: AAM61437.1 .
    PIRi F86355.
    RefSeqi NP_564165.1. NM_102079.4. [Q9LME4-1 ]
    NP_973883.1. NM_202154.2. [Q9LME4-2 ]
    UniGenei At.41611.

    3D structure databases

    ProteinModelPortali Q9LME4.
    SMRi Q9LME4. Positions 34-281.
    ModBasei Search...

    Protein-protein interaction databases

    BioGridi 24074. 2 interactions.
    IntActi Q9LME4. 2 interactions.

    Proteomic databases

    PaxDbi Q9LME4.
    PRIDEi Q9LME4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G22280.1 ; AT1G22280.1 ; AT1G22280 . [Q9LME4-1 ]
    GeneIDi 838835.
    KEGGi ath:AT1G22280.

    Organism-specific databases

    TAIRi AT1G22280.

    Phylogenomic databases

    eggNOGi COG0631.
    HOGENOMi HOG000233896.
    InParanoidi Q9LME4.
    PhylomeDBi Q9LME4.

    Enzyme and pathway databases

    BioCyci ARA:AT1G22280-MONOMER.
    ARA:GQT-527-MONOMER.

    Gene expression databases

    ArrayExpressi Q9LME4.
    Genevestigatori Q9LME4.

    Family and domain databases

    Gene3Di 3.60.40.10. 1 hit.
    InterProi IPR001932. PP2C-like_dom.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 1 hit.
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
      Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
      Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    6. "Identification of phytochrome-interacting protein candidates in Arabidopsis thaliana by co-immunoprecipitation coupled with MALDI-TOF MS."
      Phee B.-K., Shin D.H., Cho J.-H., Kim S.-H., Kim J.-I., Lee Y.-H., Jeon J.-S., Bhoo S.H., Hahn T.-R.
      Proteomics 6:3671-3680(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, INTERACTION WITH PHYTOCHROMES, IDENTIFICATION BY MASS SPECTROMETRY.
    7. "Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis."
      Niittylae T., Fuglsang A.T., Palmgren M.G., Frommer W.B., Schulze W.X.
      Mol. Cell. Proteomics 6:1711-1726(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Seedling.
    8. "A novel protein phosphatase indirectly regulates phytochrome-interacting factor 3 via phytochrome."
      Phee B.-K., Kim J.-I., Shin D.H., Yoo J., Park K.-J., Han Y.-J., Kwon Y.-K., Cho M.H., Jeon J.-S., Bhoo S.H., Hahn T.-R.
      Biochem. J. 415:247-255(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    9. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
      Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
      BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Root.
    11. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
      Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
      J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.
    12. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiP2C09_ARATH
    AccessioniPrimary (citable) accession number: Q9LME4
    Secondary accession number(s): Q3ED74, Q8LFF8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: October 1, 2000
    Last modified: June 11, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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