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Protein

Protein TIFY 10A

Gene

TIFY10A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Repressor of jasmonate responses. Jasmonoyl-isoleucine (JA-Ile) specifically promotes COI1-TIFY10A/JAZ1 interaction. Interacts with COI1 and inositol pentakisphosphate to form a high-affinity jasmonates coreceptor.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei204 – 2041Jasmonate; via amide nitrogen

GO - Molecular functioni

GO - Biological processi

  • defense response to bacterium Source: TAIR
  • flower development Source: TAIR
  • jasmonic acid mediated signaling pathway Source: TAIR
  • negative regulation of nucleic acid-templated transcription Source: GO_Central
  • pollen development Source: TAIR
  • regulation of cellular response to alkaline pH Source: CACAO
  • regulation of defense response Source: GO_Central
  • regulation of jasmonic acid mediated signaling pathway Source: GO_Central
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to jasmonic acid Source: TAIR
  • response to wounding Source: GO_Central
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Jasmonic acid signaling pathway, Plant defense, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TIFY 10A
Alternative name(s):
Jasmonate ZIM domain-containing protein 1
Gene namesi
Name:TIFY10A
Synonyms:JAZ1
Ordered Locus Names:At1g19180
ORF Names:T29M8.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G19180.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi202 – 22827Missing in jaz1delta3A; dominant mutation that confers jasmonate insensitivity. 1 PublicationAdd
BLAST
Mutagenesisi205 – 2062RR → AA: Loss of binding to COI1 and jasmonate insensitivity. No effect on MYC2 binding. 1 Publication
Mutagenesisi205 – 2051R → A: Loss of binding to COI1 but no effect on MYC2 binding.
Mutagenesisi206 – 2061R → A: Loss of binding to COI1but no effect on MYC2 binding.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 253253Protein TIFY 10APRO_0000300652Add
BLAST

Post-translational modificationi

Ubiquitinated. Targeted for degradation by the SCF(COI1) E3 ubiquitin ligase-proteasome pathway during jasmonate signaling.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ9LMA8.
PRIDEiQ9LMA8.

Expressioni

Tissue specificityi

Srtongly expressed in root tips.2 Publications

Inductioni

Up-regulated by jasmonate, wounding and herbivory.2 Publications

Gene expression databases

ExpressionAtlasiQ9LMA8. baseline and differential.
GenevisibleiQ9LMA8. AT.

Interactioni

Subunit structurei

Homo- and heterodimer. Interacts with TIFY10B/JAZ2, TIFY6B/JAZ3, TIFY6A/JAZ4, TIFY11A/JAZ5, TIFY11B/JAZ6, TIFY5A/JAZ8, TIFY7/JAZ9, TIFY9/JAZ10, TIFY3A/JAZ11 and TIFY3B/JAZ12. Interacts with COI1, MYC2, MYC3, MYC4, MYB21 and MYB24. Interacts (via tify domain) with AFPH2/NINJA.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
COI1O041975EBI-1388539,EBI-401159
MYC2Q392043EBI-1388539,EBI-1792336
TIFY5AQ8LBM23EBI-1388539,EBI-2312143
TIFY6BQ9LVI42EBI-1388539,EBI-1792431

Protein-protein interaction databases

BioGridi23740. 37 interactions.
DIPiDIP-39129N.
IntActiQ9LMA8. 14 interactions.
STRINGi3702.AT1G19180.1.

Structurei

Secondary structure

1
253
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi203 – 21614Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OGKX-ray2.80Q/R/S/U/V/W/X205-226[»]
3OGLX-ray3.18Q/R/S/U/V/W/X200-220[»]
3OGMX-ray3.34Q/R/S/U/V/W/X200-220[»]
4YZ6X-ray1.95B200-221[»]
ProteinModelPortaliQ9LMA8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9LMA8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini120 – 15536TifyPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi95 – 1028Poly-Ser

Domaini

The jas domain (202-226) is necessary and sufficient for interaction with COI1 and is required for TIFY 10A/JAI1 instability in response to jasmonate.2 Publications

Sequence similaritiesi

Belongs to the TIFY/JAZ family.Curated
Contains 1 tify domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IZA3. Eukaryota.
ENOG410ZDCD. LUCA.
HOGENOMiHOG000239787.
InParanoidiQ9LMA8.
KOiK13464.
OMAiEASNMES.
PhylomeDBiQ9LMA8.

Family and domain databases

InterProiIPR018467. CCT_CS.
IPR010399. Tify_dom.
[Graphical view]
PfamiPF09425. CCT_2. 1 hit.
PF06200. tify. 1 hit.
[Graphical view]
SMARTiSM00979. TIFY. 1 hit.
[Graphical view]
PROSITEiPS51320. TIFY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LMA8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSSMECSEF VGSRRFTGKK PSFSQTCSRL SQYLKENGSF GDLSLGMACK
60 70 80 90 100
PDVNGTLGNS RQPTTTMSLF PCEASNMDSM VQDVKPTNLF PRQPSFSSSS
110 120 130 140 150
SSLPKEDVLK MTQTTRSVKP ESQTAPLTIF YAGQVIVFND FSAEKAKEVI
160 170 180 190 200
NLASKGTANS LAKNQTDIRS NIATIANQVP HPRKTTTQEP IQSSPTPLTE
210 220 230 240 250
LPIARRASLH RFLEKRKDRV TSKAPYQLCD PAKASSNPQT TGNMSWLGLA

AEI
Length:253
Mass (Da):27,608
Last modified:October 1, 2000 - v1
Checksum:iEF2DAAC0CEF2DBBB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191K → N in AAM65383 (Ref. 4) Curated
Sequence conflicti31 – 311S → G in AAM65383 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC069143 Genomic DNA. Translation: AAF82229.1.
CP002684 Genomic DNA. Translation: AEE29814.1.
AF332421 mRNA. Translation: AAG48784.1.
AY039894 mRNA. Translation: AAK63998.1.
AY065146 mRNA. Translation: AAL38322.1.
AY081738 mRNA. Translation: AAL87391.1.
AY081633 mRNA. Translation: AAM10195.1.
AY058863 mRNA. Translation: AAL24250.1.
AY087830 mRNA. Translation: AAM65383.1.
PIRiC86325.
RefSeqiNP_564075.1. NM_101776.2. [Q9LMA8-1]
UniGeneiAt.22658.

Genome annotation databases

EnsemblPlantsiAT1G19180.1; AT1G19180.1; AT1G19180. [Q9LMA8-1]
GeneIDi838501.
KEGGiath:AT1G19180.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC069143 Genomic DNA. Translation: AAF82229.1.
CP002684 Genomic DNA. Translation: AEE29814.1.
AF332421 mRNA. Translation: AAG48784.1.
AY039894 mRNA. Translation: AAK63998.1.
AY065146 mRNA. Translation: AAL38322.1.
AY081738 mRNA. Translation: AAL87391.1.
AY081633 mRNA. Translation: AAM10195.1.
AY058863 mRNA. Translation: AAL24250.1.
AY087830 mRNA. Translation: AAM65383.1.
PIRiC86325.
RefSeqiNP_564075.1. NM_101776.2. [Q9LMA8-1]
UniGeneiAt.22658.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OGKX-ray2.80Q/R/S/U/V/W/X205-226[»]
3OGLX-ray3.18Q/R/S/U/V/W/X200-220[»]
3OGMX-ray3.34Q/R/S/U/V/W/X200-220[»]
4YZ6X-ray1.95B200-221[»]
ProteinModelPortaliQ9LMA8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23740. 37 interactions.
DIPiDIP-39129N.
IntActiQ9LMA8. 14 interactions.
STRINGi3702.AT1G19180.1.

Proteomic databases

PaxDbiQ9LMA8.
PRIDEiQ9LMA8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G19180.1; AT1G19180.1; AT1G19180. [Q9LMA8-1]
GeneIDi838501.
KEGGiath:AT1G19180.

Organism-specific databases

TAIRiAT1G19180.

Phylogenomic databases

eggNOGiENOG410IZA3. Eukaryota.
ENOG410ZDCD. LUCA.
HOGENOMiHOG000239787.
InParanoidiQ9LMA8.
KOiK13464.
OMAiEASNMES.
PhylomeDBiQ9LMA8.

Miscellaneous databases

EvolutionaryTraceiQ9LMA8.
PROiQ9LMA8.

Gene expression databases

ExpressionAtlasiQ9LMA8. baseline and differential.
GenevisibleiQ9LMA8. AT.

Family and domain databases

InterProiIPR018467. CCT_CS.
IPR010399. Tify_dom.
[Graphical view]
PfamiPF09425. CCT_2. 1 hit.
PF06200. tify. 1 hit.
[Graphical view]
SMARTiSM00979. TIFY. 1 hit.
[Graphical view]
PROSITEiPS51320. TIFY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "JAZ repressor proteins are targets of the SCF(COI1) complex during jasmonate signalling."
    Thines B., Katsir L., Melotto M., Niu Y., Mandaokar A., Liu G., Nomura K., He S.Y., Howe G.A., Browse J.
    Nature 448:661-665(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF 202-PRO--LEU-228, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY JASMONATE.
  6. Cited for: FUNCTION, INTERACTION WITH COI1 AND MYC2, GENE FAMILY, NOMENCLATURE.
  7. "A downstream mediator in the growth repression limb of the jasmonate pathway."
    Yan Y., Stolz S., Chetelat A., Reymond P., Pagni M., Dubugnon L., Farmer E.E.
    Plant Cell 19:2470-2483(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.
  8. Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
  9. "A critical role of two positively charged amino acids in the Jas motif of Arabidopsis JAZ proteins in mediating coronatine- and jasmonoyl isoleucine-dependent interactions with the COI1 F-box protein."
    Melotto M., Mecey C., Niu Y., Chung H.S., Katsir L., Yao J., Zeng W., Thines B., Staswick P., Browse J., Howe G.A., He S.Y.
    Plant J. 55:979-988(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH COI1 AND MYC2, DOMAIN, MUTAGENESIS OF 205-ARG-ARG-206.
  10. "Regulation and function of Arabidopsis JASMONATE ZIM-domain genes in response to wounding and herbivory."
    Chung H.S., Koo A.J., Gao X., Jayanty S., Thines B., Jones A.D., Howe G.A.
    Plant Physiol. 146:952-964(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY WOUNDING AND HERBIVORY.
  11. "A critical role for the TIFY motif in repression of jasmonate signaling by a stabilized splice variant of the JASMONATE ZIM-domain protein JAZ10 in Arabidopsis."
    Chung H.S., Howe G.A.
    Plant Cell 21:131-145(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TIFY10B/JAZ2; TIFY6B/JAZ3; TIFY6A/JAZ4; TIFY11A/JAZ5; TIFY11B/JAZ6; TIFY5A/JAZ8; TIFY7/JAZ9; TIFY9/JAZ10; TIFY3A/JAZ11 AND TIFY3B/JAZ12, SUBUNIT.
  12. "The ZIM domain mediates homo- and heteromeric interactions between Arabidopsis JAZ proteins."
    Chini A., Fonseca S., Chico J.M., Fernandez-Calvo P., Solano R.
    Plant J. 59:77-87(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MYC2, SUBUNIT.
  13. Cited for: INTERACTION WITH TIFY3B/JAZ12; ATR2/MYC3; COI1 AND AFPH2/NINJA.
  14. "Characterization of JAZ-interacting bHLH transcription factors that regulate jasmonate responses in Arabidopsis."
    Niu Y., Figueroa P., Browse J.
    J. Exp. Bot. 62:2143-2154(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MYC2; MYC3; MYC4 AND AFPH2.
  15. "The Jasmonate-ZIM domain proteins interact with the R2R3-MYB transcription factors MYB21 and MYB24 to affect Jasmonate-regulated stamen development in Arabidopsis."
    Song S., Qi T., Huang H., Ren Q., Wu D., Chang C., Peng W., Liu Y., Peng J., Xie D.
    Plant Cell 23:1000-1013(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MYB21 AND MYB24.
  16. Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) OF 200-226 IN COMPLEX WITH JASMONOYL-ISOLEUCINE AND CORONATINE, INTERACTION WITH COI1.

Entry informationi

Entry nameiTI10A_ARATH
AccessioniPrimary (citable) accession number: Q9LMA8
Secondary accession number(s): Q8LAG5, Q93Z09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 1, 2000
Last modified: April 13, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.