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Q9LM33

- MPK8_ARATH

UniProt

Q9LM33 - MPK8_ARATH

Protein

Mitogen-activated protein kinase 8

Gene

MPK8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (11 Jul 2006)
      Previous versions | rss
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    Functioni

    MKK3-MPK8 and CAMs-MPK8 modules negatively regulates ROS accumulation through controlling expression of the RBOHD gene during wounding.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation By similarity. Activated by two independent mechanisms, the binding of CAMs in a calcium-dependent manner and the phosphorylation by MAP kinase kinase MKK3. Activated in response to mechanical wounding, hydrogen peroxide and jasmonic acid (JA).By similarity1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei133 – 1331ATPPROSITE-ProRule annotation
    Active sitei230 – 2301Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi110 – 1189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calmodulin binding Source: TAIR
    3. MAP kinase activity Source: TAIR

    GO - Biological processi

    1. MAPK cascade Source: GOC
    2. response to hydrogen peroxide Source: TAIR
    3. response to jasmonic acid Source: TAIR
    4. response to reactive oxygen species Source: TAIR
    5. response to wounding Source: TAIR

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G18150-MONOMER.
    ARA:GQT-1005-MONOMER.
    ARA:GQT-1590-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 8 (EC:2.7.11.24)
    Short name:
    AtMPK8
    Short name:
    MAP kinase 8
    Gene namesi
    Name:MPK8
    Ordered Locus Names:At1g18150
    ORF Names:T10F20.15, T10O22.12
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G18150.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: TAIR

    Pathology & Biotechi

    Disruption phenotypei

    More succeptible to oxidative stress.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi133 – 1331K → M: Abolishes CAM4-dependent activity. 1 Publication
    Mutagenesisi134 – 1341K → R: Abolishes CAM4-dependent activity. 1 Publication
    Mutagenesisi266 – 2661T → A: Do not affect CAM4-dependent activity; when associated with F-268. 1 Publication
    Mutagenesisi268 – 2681Y → F: Do not affect CAM4-dependent activity; when associated with A-266. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 589589Mitogen-activated protein kinase 8PRO_0000245808Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei266 – 2661PhosphothreonineBy similarity
    Modified residuei268 – 2681PhosphotyrosineBy similarity
    Modified residuei271 – 2711PhosphothreonineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-266 and Tyr-268, which activates the enzyme By similarity. Autophosphorylated.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9LM33.
    PRIDEiQ9LM33.

    Expressioni

    Tissue specificityi

    Ubiquitous.1 Publication

    Gene expression databases

    GenevestigatoriQ9LM33.

    Interactioni

    Subunit structurei

    Interacts with CAM3, CAM4 AND CAM7 in an calcium-dependent manner.1 Publication

    Protein-protein interaction databases

    BioGridi23633. 2 interactions.
    IntActiQ9LM33. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LM33.
    SMRiQ9LM33. Positions 76-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini104 – 395292Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi266 – 2683TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    InParanoidiQ9LM33.
    OMAiNSEREPT.
    PhylomeDBiQ9LM33.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9LM33-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGGGGNLVDG VRRWLFQRPS SSSSSSSSNN NNNNHEQPIF NSSSFSSSSN    50
    PNHSANSGEL IIEEDLDFSG LTLINVPKRN HLPMDPHKKG ETEFFTEYGE 100
    ANRYQIQEVV GKGSYGVVAS AVDSHTGERV AIKKINDVFE HVSDATRILR 150
    EIKLLRLLRH PDVVEIKHIM LPPSRREFRD IYVVFELMES DLHQVIKAND 200
    DLTPEHYQFF LYQLLRGLKY VHAANVFHRD LKPKNILANA DCKLKICDFG 250
    LARVSFNDAP TAIFWTDYVA TRWYRAPELC GSFFSKYTPA IDIWSVGCIF 300
    AEMLLGKPLF PGKNVVHQLD LMTDFLGTPP PESISRIRNE KARRYLSSMR 350
    KKQPVPFSHK FPKADPLALR LLERLLAFDP KDRASAEDAL ADPYFSGLSN 400
    SEREPTTQPI SKLEFDFERK KLVKDDVREL IYREILEYHP QMLEEYLRGG 450
    DQLSFMYPSG VDRFKRQFAH LEENQGKPGA AGGGRSTALH RHHASLPRER 500
    VPAPNGETAE ESSDVERRAA AAVASTLESE EADNGGGYSA RNLMKSASIS 550
    GSKCIGVQSK TDKEDTIAEE EDNETVAELT DKVASLHNS 589
    Length:589
    Mass (Da):66,231
    Last modified:July 11, 2006 - v2
    Checksum:i96769BA5E164BD74
    GO

    Sequence cautioni

    The sequence BAA92222.1 differs from that shown. Reason: Frameshift at position 532.
    The sequence AAF78388.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti179 – 1802RD → PH in BAA92222. 1 PublicationCurated
    Sequence conflicti365 – 3651D → E in BAA92222. 1 PublicationCurated
    Sequence conflicti431 – 4311I → L in BAA92222. 1 PublicationCurated
    Sequence conflicti479 – 4791G → V in BAA92222. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB038693 mRNA. Translation: BAA92222.1. Frameshift.
    AC034107 Genomic DNA. Translation: AAF97831.1.
    AC069551 Genomic DNA. Translation: AAF78388.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE29679.1.
    CP002684 Genomic DNA. Translation: AEE29680.1.
    CP002684 Genomic DNA. Translation: AEE29681.1.
    AY045931 mRNA. Translation: AAK76605.1.
    AY142618 mRNA. Translation: AAN13187.1.
    RefSeqiNP_001185027.1. NM_001198098.1.
    NP_173253.1. NM_101675.3.
    NP_849685.1. NM_179354.2.
    UniGeneiAt.15885.

    Genome annotation databases

    EnsemblPlantsiAT1G18150.1; AT1G18150.1; AT1G18150.
    AT1G18150.2; AT1G18150.2; AT1G18150.
    AT1G18150.3; AT1G18150.3; AT1G18150.
    GeneIDi838394.
    KEGGiath:AT1G18150.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB038693 mRNA. Translation: BAA92222.1 . Frameshift.
    AC034107 Genomic DNA. Translation: AAF97831.1 .
    AC069551 Genomic DNA. Translation: AAF78388.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE29679.1 .
    CP002684 Genomic DNA. Translation: AEE29680.1 .
    CP002684 Genomic DNA. Translation: AEE29681.1 .
    AY045931 mRNA. Translation: AAK76605.1 .
    AY142618 mRNA. Translation: AAN13187.1 .
    RefSeqi NP_001185027.1. NM_001198098.1.
    NP_173253.1. NM_101675.3.
    NP_849685.1. NM_179354.2.
    UniGenei At.15885.

    3D structure databases

    ProteinModelPortali Q9LM33.
    SMRi Q9LM33. Positions 76-441.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 23633. 2 interactions.
    IntActi Q9LM33. 1 interaction.

    Proteomic databases

    PaxDbi Q9LM33.
    PRIDEi Q9LM33.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G18150.1 ; AT1G18150.1 ; AT1G18150 .
    AT1G18150.2 ; AT1G18150.2 ; AT1G18150 .
    AT1G18150.3 ; AT1G18150.3 ; AT1G18150 .
    GeneIDi 838394.
    KEGGi ath:AT1G18150.

    Organism-specific databases

    GeneFarmi 851. 89.
    TAIRi AT1G18150.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    InParanoidi Q9LM33.
    OMAi NSEREPT.
    PhylomeDBi Q9LM33.

    Enzyme and pathway databases

    BioCyci ARA:AT1G18150-MONOMER.
    ARA:GQT-1005-MONOMER.
    ARA:GQT-1590-MONOMER.

    Gene expression databases

    Genevestigatori Q9LM33.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arabidopsis thaliana mRNA for MAP kinase."
      Mizoguchi T., Ichimura K., Shinozaki K.
      Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
      MAPK group
      Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    6. Cited for: GENE FAMILY.
    7. "Calmodulin-dependent activation of MAP kinase for ROS homeostasis in Arabidopsis."
      Takahashi F., Mizoguchi T., Yoshida R., Ichimura K., Shinozaki K.
      Mol. Cell 41:649-660(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INTERACTION WITH CAM3; CAM4 AND CAM7, AUTOPHOSPHORYLATION, MUTAGENESIS OF LYS-133; LYS-134; THR-266 AND TYR-268.

    Entry informationi

    Entry nameiMPK8_ARATH
    AccessioniPrimary (citable) accession number: Q9LM33
    Secondary accession number(s): Q9LMS2, Q9MB23
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: July 11, 2006
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3