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Q9LM33 (MPK8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 8

Short name=AtMPK8
Short name=MAP kinase 8
EC=2.7.11.24
Gene names
Name:MPK8
Ordered Locus Names:At1g18150
ORF Names:T10F20.15, T10O22.12
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length589 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

MKK3-MPK8 and CAMs-MPK8 modules negatively regulates ROS accumulation through controlling expression of the RBOHD gene during wounding. Ref.7

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity. Activated by two independent mechanisms, the binding of CAMs in a calcium-dependent manner and the phosphorylation by MAP kinase kinase MKK3. Activated in response to mechanical wounding, hydrogen peroxide and jasmonic acid (JA). Ref.7

Subunit structure

Interacts with CAM3, CAM4 AND CAM7 in an calcium-dependent manner. Ref.7

Tissue specificity

Ubiquitous. Ref.7

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-266 and Tyr-268, which activates the enzyme By similarity. Autophosphorylated. Ref.7

Disruption phenotype

More succeptible to oxidative stress. Ref.7

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAF78388.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence BAA92222.1 differs from that shown. Reason: Frameshift at position 532.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 589589Mitogen-activated protein kinase 8
PRO_0000245808

Regions

Domain104 – 395292Protein kinase
Nucleotide binding110 – 1189ATP By similarity
Motif266 – 2683TXY

Sites

Active site2301Proton acceptor By similarity
Binding site1331ATP By similarity

Amino acid modifications

Modified residue2661Phosphothreonine By similarity
Modified residue2681Phosphotyrosine By similarity
Modified residue2711Phosphothreonine By similarity

Experimental info

Mutagenesis1331K → M: Abolishes CAM4-dependent activity. Ref.7
Mutagenesis1341K → R: Abolishes CAM4-dependent activity. Ref.7
Mutagenesis2661T → A: Do not affect CAM4-dependent activity; when associated with F-268. Ref.7
Mutagenesis2681Y → F: Do not affect CAM4-dependent activity; when associated with A-266. Ref.7
Sequence conflict179 – 1802RD → PH in BAA92222. Ref.1
Sequence conflict3651D → E in BAA92222. Ref.1
Sequence conflict4311I → L in BAA92222. Ref.1
Sequence conflict4791G → V in BAA92222. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9LM33 [UniParc].

Last modified July 11, 2006. Version 2.
Checksum: 96769BA5E164BD74

FASTA58966,231
        10         20         30         40         50         60 
MGGGGNLVDG VRRWLFQRPS SSSSSSSSNN NNNNHEQPIF NSSSFSSSSN PNHSANSGEL 

        70         80         90        100        110        120 
IIEEDLDFSG LTLINVPKRN HLPMDPHKKG ETEFFTEYGE ANRYQIQEVV GKGSYGVVAS 

       130        140        150        160        170        180 
AVDSHTGERV AIKKINDVFE HVSDATRILR EIKLLRLLRH PDVVEIKHIM LPPSRREFRD 

       190        200        210        220        230        240 
IYVVFELMES DLHQVIKAND DLTPEHYQFF LYQLLRGLKY VHAANVFHRD LKPKNILANA 

       250        260        270        280        290        300 
DCKLKICDFG LARVSFNDAP TAIFWTDYVA TRWYRAPELC GSFFSKYTPA IDIWSVGCIF 

       310        320        330        340        350        360 
AEMLLGKPLF PGKNVVHQLD LMTDFLGTPP PESISRIRNE KARRYLSSMR KKQPVPFSHK 

       370        380        390        400        410        420 
FPKADPLALR LLERLLAFDP KDRASAEDAL ADPYFSGLSN SEREPTTQPI SKLEFDFERK 

       430        440        450        460        470        480 
KLVKDDVREL IYREILEYHP QMLEEYLRGG DQLSFMYPSG VDRFKRQFAH LEENQGKPGA 

       490        500        510        520        530        540 
AGGGRSTALH RHHASLPRER VPAPNGETAE ESSDVERRAA AAVASTLESE EADNGGGYSA 

       550        560        570        580 
RNLMKSASIS GSKCIGVQSK TDKEDTIAEE EDNETVAELT DKVASLHNS 

« Hide

References

« Hide 'large scale' references
[1]"Arabidopsis thaliana mRNA for MAP kinase."
Mizoguchi T., Ichimura K., Shinozaki K.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Mitogen-activated protein kinase cascades in plants: a new nomenclature."
MAPK group
Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[6]"Ancient signals: comparative genomics of plant MAPK and MAPKK gene families."
Hamel L.P., Nicole M.C., Sritubtim S., Morency M.J., Ellis M., Ehlting J., Beaudoin N., Barbazuk B., Klessig D., Lee J., Martin G., Mundy J., Ohashi Y., Scheel D., Sheen J., Xing T., Zhang S., Seguin A., Ellis B.E.
Trends Plant Sci. 11:192-198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[7]"Calmodulin-dependent activation of MAP kinase for ROS homeostasis in Arabidopsis."
Takahashi F., Mizoguchi T., Yoshida R., Ichimura K., Shinozaki K.
Mol. Cell 41:649-660(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INTERACTION WITH CAM3; CAM4 AND CAM7, AUTOPHOSPHORYLATION, MUTAGENESIS OF LYS-133; LYS-134; THR-266 AND TYR-268.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB038693 mRNA. Translation: BAA92222.1. Frameshift.
AC034107 Genomic DNA. Translation: AAF97831.1.
AC069551 Genomic DNA. Translation: AAF78388.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29679.1.
CP002684 Genomic DNA. Translation: AEE29680.1.
CP002684 Genomic DNA. Translation: AEE29681.1.
AY045931 mRNA. Translation: AAK76605.1.
AY142618 mRNA. Translation: AAN13187.1.
RefSeqNP_001185027.1. NM_001198098.1.
NP_173253.1. NM_101675.3.
NP_849685.1. NM_179354.2.
UniGeneAt.15885.

3D structure databases

ProteinModelPortalQ9LM33.
SMRQ9LM33. Positions 76-441.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid23633. 2 interactions.
IntActQ9LM33. 1 interaction.

Proteomic databases

PaxDbQ9LM33.
PRIDEQ9LM33.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G18150.1; AT1G18150.1; AT1G18150.
AT1G18150.2; AT1G18150.2; AT1G18150.
AT1G18150.3; AT1G18150.3; AT1G18150.
GeneID838394.
KEGGath:AT1G18150.

Organism-specific databases

GeneFarm851. 89.
TAIRAT1G18150.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
InParanoidQ9LM33.
OMANSEREPT.
PhylomeDBQ9LM33.

Enzyme and pathway databases

BioCycARA:AT1G18150-MONOMER.
ARA:GQT-1005-MONOMER.
ARA:GQT-1590-MONOMER.

Gene expression databases

GenevestigatorQ9LM33.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK8_ARATH
AccessionPrimary (citable) accession number: Q9LM33
Secondary accession number(s): Q9LMS2, Q9MB23
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: June 11, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names