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Protein

Mitogen-activated protein kinase 8

Gene

MPK8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

MKK3-MPK8 and CAMs-MPK8 modules negatively regulates ROS accumulation through controlling expression of the RBOHD gene during wounding.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation (By similarity). Activated by two independent mechanisms, the binding of CAMs in a calcium-dependent manner and the phosphorylation by MAP kinase kinase MKK3. Activated in response to mechanical wounding, hydrogen peroxide and jasmonic acid (JA).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei133ATPPROSITE-ProRule annotation1
Active sitei230Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi110 – 118ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calmodulin binding Source: TAIR
  • MAP kinase activity Source: TAIR

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • regulation of gene expression Source: GO_Central
  • response to hydrogen peroxide Source: TAIR
  • response to jasmonic acid Source: TAIR
  • response to reactive oxygen species Source: TAIR
  • response to wounding Source: TAIR

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-5687128. MAPK6/MAPK4 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 8 (EC:2.7.11.24)
Short name:
AtMPK8
Short name:
MAP kinase 8
Gene namesi
Name:MPK8
Ordered Locus Names:At1g18150
ORF Names:T10F20.15, T10O22.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRilocus:2194040. AT1G18150.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nucleus Source: GO_Central
  • plasma membrane Source: TAIR

Pathology & Biotechi

Disruption phenotypei

More succeptible to oxidative stress.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi133K → M: Abolishes CAM4-dependent activity. 1 Publication1
Mutagenesisi134K → R: Abolishes CAM4-dependent activity. 1 Publication1
Mutagenesisi266T → A: Do not affect CAM4-dependent activity; when associated with F-268. 1 Publication1
Mutagenesisi268Y → F: Do not affect CAM4-dependent activity; when associated with A-266. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002458081 – 589Mitogen-activated protein kinase 8Add BLAST589

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei266PhosphothreonineBy similarity1
Modified residuei268PhosphotyrosineBy similarity1
Modified residuei271PhosphothreonineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-266 and Tyr-268, which activates the enzyme (By similarity). Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9LM33.

PTM databases

iPTMnetiQ9LM33.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

GenevisibleiQ9LM33. AT.

Interactioni

Subunit structurei

Interacts with CAM3, CAM4 AND CAM7 in an calcium-dependent manner.1 Publication

GO - Molecular functioni

  • calmodulin binding Source: TAIR

Protein-protein interaction databases

BioGridi23633. 2 interactors.
IntActiQ9LM33. 1 interactor.
STRINGi3702.AT1G18150.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LM33.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 395Protein kinasePROSITE-ProRule annotationAdd BLAST292

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi266 – 268TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ9LM33.
KOiK20538.
OMAiPRERVND.
OrthoDBiEOG093605CF.
PhylomeDBiQ9LM33.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9LM33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGGGNLVDG VRRWLFQRPS SSSSSSSSNN NNNNHEQPIF NSSSFSSSSN
60 70 80 90 100
PNHSANSGEL IIEEDLDFSG LTLINVPKRN HLPMDPHKKG ETEFFTEYGE
110 120 130 140 150
ANRYQIQEVV GKGSYGVVAS AVDSHTGERV AIKKINDVFE HVSDATRILR
160 170 180 190 200
EIKLLRLLRH PDVVEIKHIM LPPSRREFRD IYVVFELMES DLHQVIKAND
210 220 230 240 250
DLTPEHYQFF LYQLLRGLKY VHAANVFHRD LKPKNILANA DCKLKICDFG
260 270 280 290 300
LARVSFNDAP TAIFWTDYVA TRWYRAPELC GSFFSKYTPA IDIWSVGCIF
310 320 330 340 350
AEMLLGKPLF PGKNVVHQLD LMTDFLGTPP PESISRIRNE KARRYLSSMR
360 370 380 390 400
KKQPVPFSHK FPKADPLALR LLERLLAFDP KDRASAEDAL ADPYFSGLSN
410 420 430 440 450
SEREPTTQPI SKLEFDFERK KLVKDDVREL IYREILEYHP QMLEEYLRGG
460 470 480 490 500
DQLSFMYPSG VDRFKRQFAH LEENQGKPGA AGGGRSTALH RHHASLPRER
510 520 530 540 550
VPAPNGETAE ESSDVERRAA AAVASTLESE EADNGGGYSA RNLMKSASIS
560 570 580
GSKCIGVQSK TDKEDTIAEE EDNETVAELT DKVASLHNS
Length:589
Mass (Da):66,231
Last modified:July 11, 2006 - v2
Checksum:i96769BA5E164BD74
GO

Sequence cautioni

The sequence AAF78388 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAA92222 differs from that shown. Reason: Frameshift at position 532.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti179 – 180RD → PH in BAA92222 (Ref. 1) Curated2
Sequence conflicti365D → E in BAA92222 (Ref. 1) Curated1
Sequence conflicti431I → L in BAA92222 (Ref. 1) Curated1
Sequence conflicti479G → V in BAA92222 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038693 mRNA. Translation: BAA92222.1. Frameshift.
AC034107 Genomic DNA. Translation: AAF97831.1.
AC069551 Genomic DNA. Translation: AAF78388.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29679.1.
CP002684 Genomic DNA. Translation: AEE29680.1.
CP002684 Genomic DNA. Translation: AEE29681.1.
AY045931 mRNA. Translation: AAK76605.1.
AY142618 mRNA. Translation: AAN13187.1.
RefSeqiNP_001185027.1. NM_001198098.1.
NP_173253.1. NM_101675.4.
NP_849685.1. NM_179354.3.
UniGeneiAt.15885.

Genome annotation databases

EnsemblPlantsiAT1G18150.1; AT1G18150.1; AT1G18150.
AT1G18150.2; AT1G18150.2; AT1G18150.
AT1G18150.3; AT1G18150.3; AT1G18150.
GeneIDi838394.
GrameneiAT1G18150.1; AT1G18150.1; AT1G18150.
AT1G18150.2; AT1G18150.2; AT1G18150.
AT1G18150.3; AT1G18150.3; AT1G18150.
KEGGiath:AT1G18150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038693 mRNA. Translation: BAA92222.1. Frameshift.
AC034107 Genomic DNA. Translation: AAF97831.1.
AC069551 Genomic DNA. Translation: AAF78388.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29679.1.
CP002684 Genomic DNA. Translation: AEE29680.1.
CP002684 Genomic DNA. Translation: AEE29681.1.
AY045931 mRNA. Translation: AAK76605.1.
AY142618 mRNA. Translation: AAN13187.1.
RefSeqiNP_001185027.1. NM_001198098.1.
NP_173253.1. NM_101675.4.
NP_849685.1. NM_179354.3.
UniGeneiAt.15885.

3D structure databases

ProteinModelPortaliQ9LM33.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23633. 2 interactors.
IntActiQ9LM33. 1 interactor.
STRINGi3702.AT1G18150.1.

PTM databases

iPTMnetiQ9LM33.

Proteomic databases

PaxDbiQ9LM33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G18150.1; AT1G18150.1; AT1G18150.
AT1G18150.2; AT1G18150.2; AT1G18150.
AT1G18150.3; AT1G18150.3; AT1G18150.
GeneIDi838394.
GrameneiAT1G18150.1; AT1G18150.1; AT1G18150.
AT1G18150.2; AT1G18150.2; AT1G18150.
AT1G18150.3; AT1G18150.3; AT1G18150.
KEGGiath:AT1G18150.

Organism-specific databases

AraportiAT1G18150.
TAIRilocus:2194040. AT1G18150.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ9LM33.
KOiK20538.
OMAiPRERVND.
OrthoDBiEOG093605CF.
PhylomeDBiQ9LM33.

Enzyme and pathway databases

ReactomeiR-ATH-5687128. MAPK6/MAPK4 signaling.

Miscellaneous databases

PROiQ9LM33.

Gene expression databases

GenevisibleiQ9LM33. AT.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMPK8_ARATH
AccessioniPrimary (citable) accession number: Q9LM33
Secondary accession number(s): Q9LMS2, Q9MB23
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: March 15, 2017
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.