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Protein

Cycloartenol-C-24-methyltransferase

Gene

SMT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol.

Catalytic activityi

S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.

Pathwayi

GO - Molecular functioni

  1. sterol 24-C-methyltransferase activity Source: TAIR

GO - Biological processi

  1. embryo development ending in seed dormancy Source: TAIR
  2. ergosterol biosynthetic process Source: GO_Central
  3. methylation Source: GO_Central
  4. sterol biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT5G13710-MONOMER.
ARA:GQT-948-MONOMER.
MetaCyc:AT5G13710-MONOMER.
BRENDAi2.1.1.41. 399.
UniPathwayiUPA00766.

Names & Taxonomyi

Protein namesi
Recommended name:
Cycloartenol-C-24-methyltransferase (EC:2.1.1.41)
Alternative name(s):
24-sterol C-methyltransferase 1
Short name:
Sterol C-methyltransferase 1
Protein CEPHALOPOD
Protein STEROL METHYLTRANSFERASE 1
Gene namesi
Name:SMT1
Synonyms:CPH
Ordered Locus Names:At5g13710
ORF Names:MSH12.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G13710.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: TAIR
  2. plasmodesma Source: TAIR
  3. vacuole Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336Cycloartenol-C-24-methyltransferasePRO_0000124800Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9LM02.
PRIDEiQ9LM02.

Expressioni

Tissue specificityi

Highly expressed in vascular tissue, mature leaves and in regions undergoing cellular expansion.

Gene expression databases

GenevestigatoriQ9LM02.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliQ9LM02.
SMRiQ9LM02. Positions 62-202.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0500.
HOGENOMiHOG000171097.
InParanoidiQ9LM02.
KOiK00559.
OMAiFHFCRFS.
PhylomeDBiQ9LM02.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013216. Methyltransf_11.
IPR030384. MeTrfase_SMT.
IPR029063. SAM-dependent_MTases.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LM02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLASNLGGK IDKSDVLTAV EKYEQYHVFH GGNEEERKAN YTDMVNKYYD
60 70 80 90 100
LATSFYEYGW GESFHFAQRW KGESLRESIK RHEHFLALQL GIQPGQKVLD
110 120 130 140 150
VGCGIGGPLR EIARFSNSVV TGLNNNEYQI TRGKELNRLA GVDKTCNFVK
160 170 180 190 200
ADFMKMPFPE NSFDAVYAIE ATCHAPDAYG CYKEIYRVLK PGQCFAAYEW
210 220 230 240 250
CMTDAFDPDN AEHQKIKGEI EIGDGLPDIR LTTKCLEALK QAGFEVIWEK
260 270 280 290 300
DLAKDSPVPW YLPLDKNHFS LSSFRLTAVG RFITKNMVKI LEYIRLAPQG
310 320 330
SQRVSNFLEQ AAEGLVDGGR REIFTPMYFF LARKPE
Length:336
Mass (Da):38,268
Last modified:October 1, 2000 - v1
Checksum:i4649BB3868DE1CE9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti119 – 1191V → A in AAM53553 (PubMed:12100483).Curated
Sequence conflicti162 – 1621S → N in AAM53553 (PubMed:12100483).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090372 mRNA. Translation: AAF78847.1.
AF195648 mRNA. Translation: AAG28462.1.
AF494289 mRNA. Translation: AAM53553.1.
AB006704 Genomic DNA. Translation: BAB08698.1.
CP002688 Genomic DNA. Translation: AED91930.1.
CP002688 Genomic DNA. Translation: AED91931.1.
AY120716 mRNA. Translation: AAM53274.1.
BT000058 mRNA. Translation: AAN15377.1.
RefSeqiNP_001078579.1. NM_001085110.1.
NP_196875.1. NM_121374.3.
UniGeneiAt.23211.

Genome annotation databases

EnsemblPlantsiAT5G13710.1; AT5G13710.1; AT5G13710.
AT5G13710.2; AT5G13710.2; AT5G13710.
GeneIDi831216.
KEGGiath:AT5G13710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090372 mRNA. Translation: AAF78847.1.
AF195648 mRNA. Translation: AAG28462.1.
AF494289 mRNA. Translation: AAM53553.1.
AB006704 Genomic DNA. Translation: BAB08698.1.
CP002688 Genomic DNA. Translation: AED91930.1.
CP002688 Genomic DNA. Translation: AED91931.1.
AY120716 mRNA. Translation: AAM53274.1.
BT000058 mRNA. Translation: AAN15377.1.
RefSeqiNP_001078579.1. NM_001085110.1.
NP_196875.1. NM_121374.3.
UniGeneiAt.23211.

3D structure databases

ProteinModelPortaliQ9LM02.
SMRiQ9LM02. Positions 62-202.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiQ9LM02.
ChEMBLiCHEMBL4264.

Proteomic databases

PaxDbiQ9LM02.
PRIDEiQ9LM02.

Protocols and materials databases

DNASUi831216.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G13710.1; AT5G13710.1; AT5G13710.
AT5G13710.2; AT5G13710.2; AT5G13710.
GeneIDi831216.
KEGGiath:AT5G13710.

Organism-specific databases

TAIRiAT5G13710.

Phylogenomic databases

eggNOGiCOG0500.
HOGENOMiHOG000171097.
InParanoidiQ9LM02.
KOiK00559.
OMAiFHFCRFS.
PhylomeDBiQ9LM02.

Enzyme and pathway databases

UniPathwayiUPA00766.
BioCyciARA:AT5G13710-MONOMER.
ARA:GQT-948-MONOMER.
MetaCyc:AT5G13710-MONOMER.
BRENDAi2.1.1.41. 399.

Gene expression databases

GenevestigatoriQ9LM02.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013216. Methyltransf_11.
IPR030384. MeTrfase_SMT.
IPR029063. SAM-dependent_MTases.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Schaeffer A., Schaller H., Benveniste P.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sterol methyltransferase 1 controls the level of cholesterol in plants."
    Diener A.C., Li H., Zhou W.-X., Whoriskey W.J., Nes W.D., Fink G.R.
    Plant Cell 12:853-870(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  3. "Interactions between sterol biosynthesis genes in embryonic development of Arabidopsis."
    Schrick K., Mayer U., Martin G., Bellini C., Kuhnt C., Schmidt J., Juergens G.
    Plant J. 31:61-73(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Landsberg erecta.
  4. "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones."
    Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:291-300(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSMT1_ARATH
AccessioniPrimary (citable) accession number: Q9LM02
Secondary accession number(s): Q8LKW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2000
Last modified: April 1, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.