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Protein

Cycloartenol-C-24-methyltransferase

Gene

SMT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol.

Catalytic activityi

S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.

Pathwayi: sterol biosynthesis

This protein is involved in the pathway sterol biosynthesis, which is part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway sterol biosynthesis and in Steroid biosynthesis.

GO - Molecular functioni

  • sterol 24-C-methyltransferase activity Source: TAIR

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • sterol biosynthetic process Source: TAIR

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT5G13710-MONOMER
MetaCyc:AT5G13710-MONOMER
BRENDAi2.1.1.41 399
UniPathwayiUPA00766

Names & Taxonomyi

Protein namesi
Recommended name:
Cycloartenol-C-24-methyltransferase (EC:2.1.1.41)
Alternative name(s):
24-sterol C-methyltransferase 1
Short name:
Sterol C-methyltransferase 1
Protein CEPHALOPOD
Protein STEROL METHYLTRANSFERASE 1
Gene namesi
Name:SMT1
Synonyms:CPH
Ordered Locus Names:At5g13710
ORF Names:MSH12.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G13710
TAIRilocus:2173229 AT5G13710

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4264

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001248001 – 336Cycloartenol-C-24-methyltransferaseAdd BLAST336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9LM02
PRIDEiQ9LM02

PTM databases

iPTMnetiQ9LM02

Expressioni

Tissue specificityi

Highly expressed in vascular tissue, mature leaves and in regions undergoing cellular expansion.

Gene expression databases

ExpressionAtlasiQ9LM02 baseline and differential
GenevisibleiQ9LM02 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G13710.1

Chemistry databases

BindingDBiQ9LM02

Structurei

3D structure databases

ProteinModelPortaliQ9LM02
SMRiQ9LM02
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1269 Eukaryota
ENOG410XP5G LUCA
HOGENOMiHOG000171097
InParanoidiQ9LM02
KOiK00559
OMAiGIPKMYK
OrthoDBiEOG09360F3P
PhylomeDBiQ9LM02

Family and domain databases

InterProiView protein in InterPro
IPR013216 Methyltransf_11
IPR030384 MeTrfase_SMT
IPR029063 SAM-dependent_MTases
IPR013705 Sterol_MeTrfase_C
PfamiView protein in Pfam
PF08241 Methyltransf_11, 1 hit
PF08498 Sterol_MT_C, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51685 SAM_MT_ERG6_SMT, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LM02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLASNLGGK IDKSDVLTAV EKYEQYHVFH GGNEEERKAN YTDMVNKYYD
60 70 80 90 100
LATSFYEYGW GESFHFAQRW KGESLRESIK RHEHFLALQL GIQPGQKVLD
110 120 130 140 150
VGCGIGGPLR EIARFSNSVV TGLNNNEYQI TRGKELNRLA GVDKTCNFVK
160 170 180 190 200
ADFMKMPFPE NSFDAVYAIE ATCHAPDAYG CYKEIYRVLK PGQCFAAYEW
210 220 230 240 250
CMTDAFDPDN AEHQKIKGEI EIGDGLPDIR LTTKCLEALK QAGFEVIWEK
260 270 280 290 300
DLAKDSPVPW YLPLDKNHFS LSSFRLTAVG RFITKNMVKI LEYIRLAPQG
310 320 330
SQRVSNFLEQ AAEGLVDGGR REIFTPMYFF LARKPE
Length:336
Mass (Da):38,268
Last modified:October 1, 2000 - v1
Checksum:i4649BB3868DE1CE9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119V → A in AAM53553 (PubMed:12100483).Curated1
Sequence conflicti162S → N in AAM53553 (PubMed:12100483).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090372 mRNA Translation: AAF78847.1
AF195648 mRNA Translation: AAG28462.1
AF494289 mRNA Translation: AAM53553.1
AB006704 Genomic DNA Translation: BAB08698.1
CP002688 Genomic DNA Translation: AED91930.1
CP002688 Genomic DNA Translation: AED91931.1
AY120716 mRNA Translation: AAM53274.1
BT000058 mRNA Translation: AAN15377.1
RefSeqiNP_001078579.1, NM_001085110.1
NP_196875.1, NM_121374.4
UniGeneiAt.23211

Genome annotation databases

EnsemblPlantsiAT5G13710.1; AT5G13710.1; AT5G13710
AT5G13710.2; AT5G13710.2; AT5G13710
GeneIDi831216
GrameneiAT5G13710.1; AT5G13710.1; AT5G13710
AT5G13710.2; AT5G13710.2; AT5G13710
KEGGiath:AT5G13710

Similar proteinsi

Entry informationi

Entry nameiSMT1_ARATH
AccessioniPrimary (citable) accession number: Q9LM02
Secondary accession number(s): Q8LKW1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health