Reviewed, UniProtKB/Swiss-Prot Q9LM02 (SMT1_ARATH)

Names and origin

Protein names

Recommended name:
    Cycloartenol-C-24-methyltransferase
    EC=2.1.1.41
Alternative name(s):
    24-sterol C-methyltransferase 1
      Short name=Sterol C-methyltransferase 1
    Protein STEROL METHYLTRANSFERASE 1
    Protein CEPHALOPOD

Gene names

Name:	SMT1
Synonyms:	CPH
Ordered Locus Names:	At5g13710
ORF Names:	MSH12.18

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol.
Catalytic activity	S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.
Pathway	Steroid biosynthesis; sterol biosynthesis.
Tissue specificity	Highly expressed in vascular tissue, mature leaves and in regions undergoing cellular expansion.
Sequence similarities	Belongs to the methyltransferase superfamily. ERG6 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in seed dormancy Ref.2 Inferred from mutant phenotype. Source: TAIR sterol biosynthetic process Ref.2 Inferred from mutant phenotype. Source: TAIR
Cellular component	vacuole Inferred from direct assay. Source: TAIR
Molecular function	sterol 24-C-methyltransferase activity Ref.2 Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Cycloartenol-C-24-methyltransferase		PRO_0000124800
Experimental info
	Sequence conflict	119	1	V → A in AAM53553. Ref.3
	Sequence conflict	162	1	S → N in AAM53553. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9LM02-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 4649BB3868DE1CE9	FASTA	336	38,268
	10 20 30 40 50 60 MDLASNLGGK IDKSDVLTAV EKYEQYHVFH GGNEEERKAN YTDMVNKYYD LATSFYEYGW 70 80 90 100 110 120 GESFHFAQRW KGESLRESIK RHEHFLALQL GIQPGQKVLD VGCGIGGPLR EIARFSNSVV 130 140 150 160 170 180 TGLNNNEYQI TRGKELNRLA GVDKTCNFVK ADFMKMPFPE NSFDAVYAIE ATCHAPDAYG 190 200 210 220 230 240 CYKEIYRVLK PGQCFAAYEW CMTDAFDPDN AEHQKIKGEI EIGDGLPDIR LTTKCLEALK 250 260 270 280 290 300 QAGFEVIWEK DLAKDSPVPW YLPLDKNHFS LSSFRLTAVG RFITKNMVKI LEYIRLAPQG 310 320 330 SQRVSNFLEQ AAEGLVDGGR REIFTPMYFF LARKPE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Schaeffer A., Schaller H., Benveniste P. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sterol methyltransferase 1 controls the level of cholesterol in plants." Diener A.C., Li H., Zhou W.-X., Whoriskey W.J., Nes W.D., Fink G.R. Plant Cell 12:853-870(2000) [PubMed: 10852933] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[3]	"Interactions between sterol biosynthesis genes in embryonic development of Arabidopsis." Schrick K., Mayer U., Martin G., Bellini C., Kuhnt C., Schmidt J., Juergens G. Plant J. 31:61-73(2002) [PubMed: 12100483] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Landsberg erecta.
[4]	"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones." Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:291-300(1997) [PubMed: 9405937] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF090372 mRNA. Translation: AAF78847.1. AF195648 mRNA. Translation: AAG28462.1. AF494289 mRNA. Translation: AAM53553.1. AB006704 Genomic DNA. Translation: BAB08698.1. AY120716 mRNA. Translation: AAM53274.1. BT000058 mRNA. Translation: AAN15377.1.
IPI	IPI00522652.
RefSeq	NP_001078579.1. NP_196875.1.
UniGene	At.23211 Rsa.2611
3D structure databases
SMR	Q9LM02. Positions 43-262, 82-305.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LM02.
Proteomic databases
PRIDE	Q9LM02.
Genome annotation databases
GeneID	831216.
GenomeReviews	Gene locus AT5G13710 in contig BA000015_GR.
KEGG	ath:AT5G13710.
NMPDR	fig|3702.1.peg.23494.
Organism-specific databases
TAIR	At5g13710.
Phylogenomic databases
eggNOG	KOG1269.
HOGENOM	HBG316404.
InParanoid	Q9LM02.
OMA	FVKGDFM.
PhylomeDB	Q9LM02.
Enzyme and pathway databases
BioCyc	MetaCyc:AT5G13710-MONOMER.
BRENDA	2.1.1.41. 302.
Gene expression databases
ArrayExpress	Q9LM02.
Genevestigator	Q9LM02.
GermOnline	AT5G13710. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR013216. Methyltransf_11. IPR013705. Sterol_MeTrfase_C. [Graphical view]
Pfam	PF08241. Methyltransf_11. 1 hit. PF08498. Sterol_MT_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SMT1_ARATH

Accession

Primary (citable) accession number: Q9LM02
Secondary accession number(s): Q8LKW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)