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Q9LM02 (SMT1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cycloartenol-C-24-methyltransferase

EC=2.1.1.41
Alternative name(s):
24-sterol C-methyltransferase 1
Short name=Sterol C-methyltransferase 1
Protein CEPHALOPOD
Protein STEROL METHYLTRANSFERASE 1
Gene names
Name:SMT1
Synonyms:CPH
Ordered Locus Names:At5g13710
ORF Names:MSH12.18
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol.

Catalytic activity

S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.

Pathway

Steroid biosynthesis; sterol biosynthesis.

Tissue specificity

Highly expressed in vascular tissue, mature leaves and in regions undergoing cellular expansion.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336Cycloartenol-C-24-methyltransferase
PRO_0000124800

Amino acid modifications

Modified residue11N-acetylmethionine Ref.7

Experimental info

Sequence conflict1191V → A in AAM53553. Ref.3
Sequence conflict1621S → N in AAM53553. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9LM02 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 4649BB3868DE1CE9

FASTA33638,268
        10         20         30         40         50         60 
MDLASNLGGK IDKSDVLTAV EKYEQYHVFH GGNEEERKAN YTDMVNKYYD LATSFYEYGW 

        70         80         90        100        110        120 
GESFHFAQRW KGESLRESIK RHEHFLALQL GIQPGQKVLD VGCGIGGPLR EIARFSNSVV 

       130        140        150        160        170        180 
TGLNNNEYQI TRGKELNRLA GVDKTCNFVK ADFMKMPFPE NSFDAVYAIE ATCHAPDAYG 

       190        200        210        220        230        240 
CYKEIYRVLK PGQCFAAYEW CMTDAFDPDN AEHQKIKGEI EIGDGLPDIR LTTKCLEALK 

       250        260        270        280        290        300 
QAGFEVIWEK DLAKDSPVPW YLPLDKNHFS LSSFRLTAVG RFITKNMVKI LEYIRLAPQG 

       310        320        330 
SQRVSNFLEQ AAEGLVDGGR REIFTPMYFF LARKPE 

« Hide

References

« Hide 'large scale' references
[1]Schaeffer A., Schaller H., Benveniste P.
Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sterol methyltransferase 1 controls the level of cholesterol in plants."
Diener A.C., Li H., Zhou W.-X., Whoriskey W.J., Nes W.D., Fink G.R.
Plant Cell 12:853-870(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[3]"Interactions between sterol biosynthesis genes in embryonic development of Arabidopsis."
Schrick K., Mayer U., Martin G., Bellini C., Kuhnt C., Schmidt J., Juergens G.
Plant J. 31:61-73(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Landsberg erecta.
[4]"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones."
Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S.
DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[6]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF090372 mRNA. Translation: AAF78847.1.
AF195648 mRNA. Translation: AAG28462.1.
AF494289 mRNA. Translation: AAM53553.1.
AB006704 Genomic DNA. Translation: BAB08698.1.
CP002688 Genomic DNA. Translation: AED91930.1.
CP002688 Genomic DNA. Translation: AED91931.1.
AY120716 mRNA. Translation: AAM53274.1.
BT000058 mRNA. Translation: AAN15377.1.
RefSeqNP_001078579.1. NM_001085110.1.
NP_196875.1. NM_121374.3.
UniGeneAt.23211.

3D structure databases

ProteinModelPortalQ9LM02.
SMRQ9LM02. Positions 62-202.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBQ9LM02.
ChEMBLCHEMBL4264.

Proteomic databases

PaxDbQ9LM02.
PRIDEQ9LM02.

Protocols and materials databases

DNASU831216.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G13710.1; AT5G13710.1; AT5G13710.
AT5G13710.2; AT5G13710.2; AT5G13710.
GeneID831216.
KEGGath:AT5G13710.

Organism-specific databases

TAIRAT5G13710.

Phylogenomic databases

eggNOGCOG0500.
HOGENOMHOG000171097.
InParanoidQ9LM02.
KOK00559.
OMAFHFCRFS.
PhylomeDBQ9LM02.

Enzyme and pathway databases

BioCycARA:AT5G13710-MONOMER.
ARA:GQT-948-MONOMER.
MetaCyc:AT5G13710-MONOMER.
UniPathwayUPA00766.

Gene expression databases

GenevestigatorQ9LM02.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR025810. ERG6.
IPR013216. Methyltransf_11.
IPR029063. SAM-dependent_MTases-like.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamPF08241. Methyltransf_11. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSMT1_ARATH
AccessionPrimary (citable) accession number: Q9LM02
Secondary accession number(s): Q8LKW1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names