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Protein

Cycloartenol-C-24-methyltransferase

Gene

SMT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol.

Catalytic activityi

S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.

Pathwayi: sterol biosynthesis

This protein is involved in the pathway sterol biosynthesis, which is part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway sterol biosynthesis and in Steroid biosynthesis.

GO - Molecular functioni

  • sterol 24-C-methyltransferase activity Source: TAIR

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • sterol biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT5G13710-MONOMER.
MetaCyc:AT5G13710-MONOMER.
BRENDAi2.1.1.41. 399.
UniPathwayiUPA00766.

Names & Taxonomyi

Protein namesi
Recommended name:
Cycloartenol-C-24-methyltransferase (EC:2.1.1.41)
Alternative name(s):
24-sterol C-methyltransferase 1
Short name:
Sterol C-methyltransferase 1
Protein CEPHALOPOD
Protein STEROL METHYLTRANSFERASE 1
Gene namesi
Name:SMT1
Synonyms:CPH
Ordered Locus Names:At5g13710
ORF Names:MSH12.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G13710.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • endosome Source: GO_Central
  • plasmodesma Source: TAIR
  • trans-Golgi network Source: GO_Central
  • vacuolar membrane Source: GO_Central
  • vacuole Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4264.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001248001 – 336Cycloartenol-C-24-methyltransferaseAdd BLAST336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9LM02.
PRIDEiQ9LM02.

Expressioni

Tissue specificityi

Highly expressed in vascular tissue, mature leaves and in regions undergoing cellular expansion.

Gene expression databases

GenevisibleiQ9LM02. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G13710.1.

Chemistry databases

BindingDBiQ9LM02.

Structurei

3D structure databases

ProteinModelPortaliQ9LM02.
SMRiQ9LM02.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1269. Eukaryota.
ENOG410XP5G. LUCA.
HOGENOMiHOG000171097.
InParanoidiQ9LM02.
KOiK00559.
OMAiFHFCRFS.
OrthoDBiEOG09360F3P.
PhylomeDBiQ9LM02.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013216. Methyltransf_11.
IPR030384. MeTrfase_SMT.
IPR029063. SAM-dependent_MTases.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LM02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLASNLGGK IDKSDVLTAV EKYEQYHVFH GGNEEERKAN YTDMVNKYYD
60 70 80 90 100
LATSFYEYGW GESFHFAQRW KGESLRESIK RHEHFLALQL GIQPGQKVLD
110 120 130 140 150
VGCGIGGPLR EIARFSNSVV TGLNNNEYQI TRGKELNRLA GVDKTCNFVK
160 170 180 190 200
ADFMKMPFPE NSFDAVYAIE ATCHAPDAYG CYKEIYRVLK PGQCFAAYEW
210 220 230 240 250
CMTDAFDPDN AEHQKIKGEI EIGDGLPDIR LTTKCLEALK QAGFEVIWEK
260 270 280 290 300
DLAKDSPVPW YLPLDKNHFS LSSFRLTAVG RFITKNMVKI LEYIRLAPQG
310 320 330
SQRVSNFLEQ AAEGLVDGGR REIFTPMYFF LARKPE
Length:336
Mass (Da):38,268
Last modified:October 1, 2000 - v1
Checksum:i4649BB3868DE1CE9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119V → A in AAM53553 (PubMed:12100483).Curated1
Sequence conflicti162S → N in AAM53553 (PubMed:12100483).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090372 mRNA. Translation: AAF78847.1.
AF195648 mRNA. Translation: AAG28462.1.
AF494289 mRNA. Translation: AAM53553.1.
AB006704 Genomic DNA. Translation: BAB08698.1.
CP002688 Genomic DNA. Translation: AED91930.1.
CP002688 Genomic DNA. Translation: AED91931.1.
AY120716 mRNA. Translation: AAM53274.1.
BT000058 mRNA. Translation: AAN15377.1.
RefSeqiNP_001078579.1. NM_001085110.1.
NP_196875.1. NM_121374.4.
UniGeneiAt.23211.

Genome annotation databases

EnsemblPlantsiAT5G13710.1; AT5G13710.1; AT5G13710.
AT5G13710.2; AT5G13710.2; AT5G13710.
GeneIDi831216.
GrameneiAT5G13710.1; AT5G13710.1; AT5G13710.
AT5G13710.2; AT5G13710.2; AT5G13710.
KEGGiath:AT5G13710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090372 mRNA. Translation: AAF78847.1.
AF195648 mRNA. Translation: AAG28462.1.
AF494289 mRNA. Translation: AAM53553.1.
AB006704 Genomic DNA. Translation: BAB08698.1.
CP002688 Genomic DNA. Translation: AED91930.1.
CP002688 Genomic DNA. Translation: AED91931.1.
AY120716 mRNA. Translation: AAM53274.1.
BT000058 mRNA. Translation: AAN15377.1.
RefSeqiNP_001078579.1. NM_001085110.1.
NP_196875.1. NM_121374.4.
UniGeneiAt.23211.

3D structure databases

ProteinModelPortaliQ9LM02.
SMRiQ9LM02.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G13710.1.

Chemistry databases

BindingDBiQ9LM02.
ChEMBLiCHEMBL4264.

Proteomic databases

PaxDbiQ9LM02.
PRIDEiQ9LM02.

Protocols and materials databases

DNASUi831216.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G13710.1; AT5G13710.1; AT5G13710.
AT5G13710.2; AT5G13710.2; AT5G13710.
GeneIDi831216.
GrameneiAT5G13710.1; AT5G13710.1; AT5G13710.
AT5G13710.2; AT5G13710.2; AT5G13710.
KEGGiath:AT5G13710.

Organism-specific databases

TAIRiAT5G13710.

Phylogenomic databases

eggNOGiKOG1269. Eukaryota.
ENOG410XP5G. LUCA.
HOGENOMiHOG000171097.
InParanoidiQ9LM02.
KOiK00559.
OMAiFHFCRFS.
OrthoDBiEOG09360F3P.
PhylomeDBiQ9LM02.

Enzyme and pathway databases

UniPathwayiUPA00766.
BioCyciARA:AT5G13710-MONOMER.
MetaCyc:AT5G13710-MONOMER.
BRENDAi2.1.1.41. 399.

Miscellaneous databases

PROiQ9LM02.

Gene expression databases

GenevisibleiQ9LM02. AT.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013216. Methyltransf_11.
IPR030384. MeTrfase_SMT.
IPR029063. SAM-dependent_MTases.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSMT1_ARATH
AccessioniPrimary (citable) accession number: Q9LM02
Secondary accession number(s): Q8LKW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.