Reviewed, UniProtKB/Swiss-Prot Q9LLY4 (LPAT1_BRANA)

Names and origin

Protein names

Recommended name:
    1-acyl-sn-glycerol-3-phosphate acyltransferase 1, chloroplastic
    EC=2.3.1.51

Gene names

Name:	LPAT1
Synonyms:	ACT2, BAT2

Organism

Brassica napus (Rape)

Taxonomic identifier

3708 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating acyl moiety at the 2 position. Has preference for C-16-CoA substrates compared to C-18-CoA substrates.
Catalytic activity	Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate. Ref.1
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
Subcellular location	Plastid › chloroplast membrane; Multi-pass membrane protein. Ref.1
Tissue specificity	Ubiquitously expressed. Ref.1
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Chloroplast Membrane Plastid
Domain	Transit peptide Transmembrane
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-acylglycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 87	87	Chloroplast Potential
	Chain	88 – 344	257	1-acyl-sn-glycerol-3-phosphate acyltransferase 1, chloroplastic		PRO_0000024702
Regions
	Transmembrane	113 – 133	21	Potential
	Transmembrane	210 – 230	21	Potential
	Motif	188 – 193	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	Q9LLY4-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: B666D9C8AA09AB33	FASTA	344	37,998
	10 20 30 40 50 60 MDVASARGVS SHPPYYSKPI CSSQSSLIRI PISKGCCFAR SSNLITSLHA ASRGVTRRTS 70 80 90 100 110 120 GVQWCYRSIR FDPFKVNDKN SRTVTVRSDL SGAATPESTY PEPEIKLSSR LRGICFCLVA 130 140 150 160 170 180 GISAIVLIVL MIIGHPFVLL FDRYRRKFHH FIAKLWASIS IYPFYKTDIQ GLENLPSSDT 190 200 210 220 230 240 PCVYVSNHQS FLDIYTLLSL GQSYKFISKT GIFVIPVIGW AMSMMGVVPL KRMDPRSQVD 250 260 270 280 290 300 CLKRCMELVK KGASVFFFPE GTRSKDGRLG PFKKGAFTIA AKTGVPVVPI TLMGTGKIMP 310 320 330 340 TGSEGILNHG DVRVIIHKPI YGSKADVLCE EARNKIAESM NLLS

References

[1]

"A plastidial lysophosphatidic acid acyltransferase from oilseed rape." Bourgis F., Kader J.-C., Barret P., Renard M., Robinson D., Robinson C., Delseny M., Roscoe T.J. Plant Physiol. 120:913-922(1999) [PubMed: 10398728] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF111161 mRNA. Translation: AAF73736.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.1.51. 393.
Family and domain databases
InterPro	IPR002123. Acyltransferase. IPR004552. AGP_acyltrans. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR00530. AGP_acyltrn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPAT1_BRANA

Accession

Primary (citable) accession number: Q9LLY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)