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Protein

Pectate lyase 1

Gene
N/A
Organism
Juniperus virginiana (Eastern redcedar) (Sabina virginiana)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Has pectate lyase activity.By similarity

Catalytic activityi

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion.By similarity

Pathwayi: pectin degradation

This protein is involved in step 2 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Pectate lyase 1
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi170CalciumBy similarity1
Metal bindingi194CalciumBy similarity1
Metal bindingi198CalciumBy similarity1
Active sitei250Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
LigandCalcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Protein family/group databases

CAZyiPL1. Polysaccharide Lyase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectate lyase 1 (EC:4.2.2.2)
Alternative name(s):
Major pollen allergen Jun v 1
Allergen: Jun v 1
OrganismiJuniperus virginiana (Eastern redcedar) (Sabina virginiana)
Taxonomic identifieri39584 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesCupressaceaeJuniperus

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE of patients who are allergic to J.ashei.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3342. Jun v 1.0101.
3343. Jun v 1.0102.
431. Jun v 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 211 PublicationAdd BLAST21
ChainiPRO_000002490922 – 367Pectate lyase 1Add BLAST346

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi28 ↔ 45By similarity
Disulfide bondi128 ↔ 147By similarity
Glycosylationi148N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi178N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi306 ↔ 312By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ9LLT1.
SMRiQ9LLT1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiView protein in InterPro
IPR018082. AmbAllergen.
IPR002022. Pec_lyase.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
PfamiView protein in Pfam
PF00544. Pec_lyase_C. 1 hit.
PRINTSiPR00807. AMBALLERGEN.
SMARTiView protein in SMART
SM00656. Amb_all. 1 hit.
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LLT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPCLIAFL VFLCAIVSCC SDNPIDSCWR GDSNWGQNRM KLADCAVGFG
60 70 80 90 100
SSTMGGKGGD FYTVTSADDN PVNPTPGTLR YGATREKTLW IIFSQNMNIK
110 120 130 140 150
LKMPLYVAGH KTIDGRGADV HLGNGGPCLF MRKVSHVILH GLHIHGCNTS
160 170 180 190 200
VLGDVLVSES IGVVPVHAQD GDAITMRNVT NAWIDHNSLS DCSDGLIDVT
210 220 230 240 250
LGSTGITISN NHFFNHHKVM LLGHDDTYDD DKSMKVTVAF NQFGPNAGQR
260 270 280 290 300
MPRARYGLVH VANNNYDPWN IYAIGGSSNP TILSEGNSFT APNENYKKEV
310 320 330 340 350
TKRIGCESTS ACANWVWRST RDAFSNGAYF VSSGKIEETN IYNSNEAFKV
360
ENGNAAPQLT KNAGVVA
Length:367
Mass (Da):39,709
Last modified:October 1, 2000 - v1
Checksum:iDCBD1981A74E4711
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti209S → F1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151427 mRNA. Translation: AAF80164.1.
AF151429 mRNA. Translation: AAF80166.1.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPLY1_JUNVI
AccessioniPrimary (citable) accession number: Q9LLT1
Secondary accession number(s): P81825, Q9LLT2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: October 1, 2000
Last modified: August 30, 2017
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families