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Q9LKR3

- MD37A_ARATH

UniProt

Q9LKR3 - MD37A_ARATH

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Protein

Mediator of RNA polymerase II transcription subunit 37a

Gene
MED37A, BIP1, HSP70-11, MED37_5, At5g28540, T26D3.10, T26D3_50
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.1 Publication
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Involved in polar nuclei fusion during female gametophyte development and is essential for the regulation of endosperm nuclei proliferation.1 Publication
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage By similarity.1 Publication

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. ER-associated ubiquitin-dependent protein catabolic process Source: TAIR
  2. polar nucleus fusion Source: TAIR
  3. regulation of transcription, DNA-templated Source: UniProtKB-KW
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-238-MONOMER.
ReactomeiREACT_108190. ATF6-alpha activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 37a
Alternative name(s):
Heat shock 70 kDa protein 11
Heat shock protein 70-11
Short name:
AtHsp70-11
Luminal-binding protein 1
Short name:
AtBP1
Short name:
BiP1
Gene namesi
Name:MED37A
Synonyms:BIP1, HSP70-11, MED37_5
Ordered Locus Names:At5g28540
ORF Names:T26D3.10, T26D3_50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G28540.

Subcellular locationi

Endoplasmic reticulum lumen By similarity. Nucleus Inferred

GO - Cellular componenti

  1. cell wall Source: TAIR
  2. chloroplast Source: TAIR
  3. cytosol Source: TAIR
  4. endoplasmic reticulum Source: TAIR
  5. endoplasmic reticulum lumen Source: UniProtKB-SubCell
  6. Golgi apparatus Source: TAIR
  7. mediator complex Source: UniProtKB
  8. plasma membrane Source: TAIR
  9. plasmodesma Source: TAIR
  10. vacuolar membrane Source: TAIR
  11. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Nucleus

Pathology & Biotechi

Disruption phenotypei

Bip1 and bip2 double mutation affects the fusion of polar nuclei during female gametophyte development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed predictionAdd
BLAST
Chaini28 – 669642Mediator of RNA polymerase II transcription subunit 37aPRO_0000013588Add
BLAST

Post-translational modificationi

Ubiquitinated.

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ9LKR3.
PRIDEiQ9LKR3.

2D gel databases

SWISS-2DPAGEQ9LKR3.

Expressioni

Developmental stagei

Down-regulated during seed maturation. Up-regulated during germination.1 Publication

Inductioni

By heat shock.1 Publication

Gene expression databases

GenevestigatoriQ9LKR3.

Interactioni

Subunit structurei

Component of the Mediator complex. Interacts with PDIL1-4.3 Publications

Protein-protein interaction databases

BioGridi18223. 10 interactions.
IntActiQ9LKR3. 4 interactions.
MINTiMINT-7950246.

Structurei

3D structure databases

ProteinModelPortaliQ9LKR3.
SMRiQ9LKR3. Positions 34-652.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi666 – 6694Prevents secretion from ER Reviewed prediction

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0443.
InParanoidiQ9LKR3.
KOiK09490.
OMAiCWIALLF.
PhylomeDBiQ9LKR3.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LKR3-1 [UniParc]FASTAAdd to Basket

« Hide

MARSFGANST VVLAIIFFGC LFALSSAIEE ATKLGSVIGI DLGTTYSCVG    50
VYKNGHVEII ANDQGNRITP SWVGFTDSER LIGEAAKNQA AVNPERTVFD 100
VKRLIGRKFE DKEVQKDRKL VPYQIVNKDG KPYIQVKIKD GETKVFSPEE 150
ISAMILTKMK ETAEAYLGKK IKDAVVTVPA YFNDAQRQAT KDAGVIAGLN 200
VARIINEPTA AAIAYGLDKK GGEKNILVFD LGGGTFDVSV LTIDNGVFEV 250
LSTNGDTHLG GEDFDHRVME YFIKLIKKKH QKDISKDNKA LGKLRRECER 300
AKRALSSQHQ VRVEIESLFD GVDFSEPLTR ARFEELNNDL FRKTMGPVKK 350
AMDDAGLQKS QIDEIVLVGG STRIPKVQQL LKDFFEGKEP NKGVNPDEAV 400
AYGAAVQGGI LSGEGGDETK DILLLDVAPL TLGIETVGGV MTKLIPRNTV 450
IPTKKSQVFT TYQDQQTTVS IQVFEGERSL TKDCRLLGKF DLNGIPPAPR 500
GTPQIEVTFE VDANGILNVK AEDKASGKSE KITITNEKGR LSQEEIDRMV 550
KEAEEFAEED KKVKEKIDAR NALETYVYNM KNQVNDKDKL ADKLEGDEKE 600
KIEAATKEAL EWLDENQNSE KEEYDEKLKE VEAVCNPIIT AVYQRSGGAP 650
GGAGGESSTE EEDESHDEL 669
Length:669
Mass (Da):73,629
Last modified:October 1, 2000 - v1
Checksum:i20F38DA029F3F3F2
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti215 – 2162YG → NV in BAA13947. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D89341 Genomic DNA. Translation: BAA13947.1.
AF262043 Genomic DNA. Translation: AAF88019.1.
CP002688 Genomic DNA. Translation: AED93812.1.
BT000453 mRNA. Translation: AAN17430.1.
BT001212 mRNA. Translation: AAN65099.1.
AK221441 mRNA. Translation: BAD94482.1.
Z17760 mRNA. Translation: CAA79056.1.
RefSeqiNP_198206.1. NM_122737.3.
UniGeneiAt.23381.
At.49118.
At.49188.
At.64764.

Genome annotation databases

EnsemblPlantsiAT5G28540.1; AT5G28540.1; AT5G28540.
GeneIDi832950.
KEGGiath:AT5G28540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D89341 Genomic DNA. Translation: BAA13947.1 .
AF262043 Genomic DNA. Translation: AAF88019.1 .
CP002688 Genomic DNA. Translation: AED93812.1 .
BT000453 mRNA. Translation: AAN17430.1 .
BT001212 mRNA. Translation: AAN65099.1 .
AK221441 mRNA. Translation: BAD94482.1 .
Z17760 mRNA. Translation: CAA79056.1 .
RefSeqi NP_198206.1. NM_122737.3.
UniGenei At.23381.
At.49118.
At.49188.
At.64764.

3D structure databases

ProteinModelPortali Q9LKR3.
SMRi Q9LKR3. Positions 34-652.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 18223. 10 interactions.
IntActi Q9LKR3. 4 interactions.
MINTi MINT-7950246.

2D gel databases

SWISS-2DPAGE Q9LKR3.

Proteomic databases

PaxDbi Q9LKR3.
PRIDEi Q9LKR3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G28540.1 ; AT5G28540.1 ; AT5G28540 .
GeneIDi 832950.
KEGGi ath:AT5G28540.

Organism-specific databases

TAIRi AT5G28540.

Phylogenomic databases

eggNOGi COG0443.
InParanoidi Q9LKR3.
KOi K09490.
OMAi CWIALLF.
PhylomeDBi Q9LKR3.

Enzyme and pathway databases

BioCyci ARA:GQT-238-MONOMER.
Reactomei REACT_108190. ATF6-alpha activates chaperone genes.

Gene expression databases

Genevestigatori Q9LKR3.

Family and domain databases

Gene3Di 1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProi IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view ]
Pfami PF00012. HSP70. 1 hit.
[Graphical view ]
PRINTSi PR00301. HEATSHOCK70.
SUPFAMi SSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of two genes encoding luminal binding protein from Arabidopsis thaliana."
    Koizumi N., Sano H.
    Plant Gene Register PGR97-028
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 537-669.
    Strain: cv. Columbia.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 620-669.
    Strain: cv. Columbia.
    Tissue: Green siliques.
  7. "Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana."
    Lin B.L., Wang J.S., Liu H.C., Chen R.W., Meyer Y., Barakat A., Delseny M.
    Cell Stress Chaperones 6:201-208(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  8. "Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family."
    Sung D.Y., Vierling E., Guy C.L.
    Plant Physiol. 126:789-800(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNAK GENE SUBFAMILY, INDUCTION, DEVELOPMENTAL STAGE.
  9. "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1 as the Med25 subunit."
    Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.
    Mol. Cell 26:717-729(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, NOMENCLATURE.
  10. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
    Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
    Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
  11. "BiP-mediated polar nuclei fusion is essential for the regulation of endosperm nuclei proliferation in Arabidopsis thaliana."
    Maruyama D., Endo T., Nishikawa S.
    Proc. Natl. Acad. Sci. U.S.A. 107:1684-1689(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  12. "Protein disulfide isomerase-2 of Arabidopsis mediates protein folding and localizes to both the secretory pathway and nucleus, where it interacts with maternal effect embryo arrest factor."
    Cho E.J., Yuen C.Y., Kang B.H., Ondzighi C.A., Staehelin L.A., Christopher D.A.
    Mol. Cells 32:459-475(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PDIL1-4.
  13. "The Mediator complex in plants: structure, phylogeny, and expression profiling of representative genes in a dicot (Arabidopsis) and a monocot (rice) during reproduction and abiotic stress."
    Mathur S., Vyas S., Kapoor S., Tyagi A.K.
    Plant Physiol. 157:1609-1627(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, SUBUNIT, NOMENCLATURE.

Entry informationi

Entry nameiMD37A_ARATH
AccessioniPrimary (citable) accession number: Q9LKR3
Secondary accession number(s): Q41928, Q56Y82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 1, 2000
Last modified: September 3, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi