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Protein

Mediator of RNA polymerase II transcription subunit 37a

Gene

MED37A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.1 Publication
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Involved in polar nuclei fusion during female gametophyte development and is essential for the regulation of endosperm nuclei proliferation.1 Publication
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
  • ubiquitin-dependent ERAD pathway Source: TAIR

Keywordsi

Molecular functionChaperone
Biological processTranscription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-3371453 Regulation of HSF1-mediated heat shock response

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 37a
Alternative name(s):
Heat shock 70 kDa protein 11
Heat shock protein 70-11
Short name:
AtHsp70-11
Luminal-binding protein 1
Short name:
AtBP1
Short name:
BiP1
Gene namesi
Name:MED37A
Synonyms:BIP1, HSP70-11, MED37_5
Ordered Locus Names:At5g28540
ORF Names:T26D3.10, T26D3_50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G28540
TAIRilocus:2182783 AT5G28540

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Nucleus

Pathology & Biotechi

Disruption phenotypei

Bip1 and bip2 double mutation affects the fusion of polar nuclei during female gametophyte development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000001358828 – 669Mediator of RNA polymerase II transcription subunit 37aAdd BLAST642

Proteomic databases

PaxDbiQ9LKR3
PRIDEiQ9LKR3

2D gel databases

SWISS-2DPAGEiQ9LKR3

PTM databases

iPTMnetiQ9LKR3

Expressioni

Developmental stagei

Down-regulated during seed maturation. Up-regulated during germination.1 Publication

Inductioni

By heat shock.1 Publication

Gene expression databases

ExpressionAtlasiQ9LKR3 baseline and differential
GenevisibleiQ9LKR3 AT

Interactioni

Subunit structurei

Component of the Mediator complex (PubMed:17560376, PubMed:22021418). Interacts with PDIL1-4 and ERDJ3B (PubMed:19763086, PubMed:21909944). Interacts with BZIP28 (via C-terminus). MED37A/BIP1 and MED37B/BIP3 dissociates from BZIP28 under ER stress (PubMed:23624714).5 Publications

Protein-protein interaction databases

BioGridi18223, 17 interactors
IntActiQ9LKR3, 4 interactors
MINTiQ9LKR3
STRINGi3702.AT5G28540.1

Structurei

3D structure databases

ProteinModelPortaliQ9LKR3
SMRiQ9LKR3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi666 – 669Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0100 Eukaryota
COG0443 LUCA
HOGENOMiHOG000228135
InParanoidiQ9LKR3
KOiK09490
OMAiEWNDPSV
OrthoDBiEOG093604JO
PhylomeDBiQ9LKR3

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LKR3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARSFGANST VVLAIIFFGC LFALSSAIEE ATKLGSVIGI DLGTTYSCVG
60 70 80 90 100
VYKNGHVEII ANDQGNRITP SWVGFTDSER LIGEAAKNQA AVNPERTVFD
110 120 130 140 150
VKRLIGRKFE DKEVQKDRKL VPYQIVNKDG KPYIQVKIKD GETKVFSPEE
160 170 180 190 200
ISAMILTKMK ETAEAYLGKK IKDAVVTVPA YFNDAQRQAT KDAGVIAGLN
210 220 230 240 250
VARIINEPTA AAIAYGLDKK GGEKNILVFD LGGGTFDVSV LTIDNGVFEV
260 270 280 290 300
LSTNGDTHLG GEDFDHRVME YFIKLIKKKH QKDISKDNKA LGKLRRECER
310 320 330 340 350
AKRALSSQHQ VRVEIESLFD GVDFSEPLTR ARFEELNNDL FRKTMGPVKK
360 370 380 390 400
AMDDAGLQKS QIDEIVLVGG STRIPKVQQL LKDFFEGKEP NKGVNPDEAV
410 420 430 440 450
AYGAAVQGGI LSGEGGDETK DILLLDVAPL TLGIETVGGV MTKLIPRNTV
460 470 480 490 500
IPTKKSQVFT TYQDQQTTVS IQVFEGERSL TKDCRLLGKF DLNGIPPAPR
510 520 530 540 550
GTPQIEVTFE VDANGILNVK AEDKASGKSE KITITNEKGR LSQEEIDRMV
560 570 580 590 600
KEAEEFAEED KKVKEKIDAR NALETYVYNM KNQVNDKDKL ADKLEGDEKE
610 620 630 640 650
KIEAATKEAL EWLDENQNSE KEEYDEKLKE VEAVCNPIIT AVYQRSGGAP
660
GGAGGESSTE EEDESHDEL
Length:669
Mass (Da):73,629
Last modified:October 1, 2000 - v1
Checksum:i20F38DA029F3F3F2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti215 – 216YG → NV in BAA13947 (PubMed:11130714).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89341 Genomic DNA Translation: BAA13947.1
AF262043 Genomic DNA Translation: AAF88019.1
CP002688 Genomic DNA Translation: AED93812.1
BT000453 mRNA Translation: AAN17430.1
BT001212 mRNA Translation: AAN65099.1
AK221441 mRNA Translation: BAD94482.1
Z17760 mRNA Translation: CAA79056.1
RefSeqiNP_198206.1, NM_122737.4
UniGeneiAt.23381
At.49118
At.49188
At.64764

Genome annotation databases

EnsemblPlantsiAT5G28540.1; AT5G28540.1; AT5G28540
GeneIDi832950
GrameneiAT5G28540.1; AT5G28540.1; AT5G28540
KEGGiath:AT5G28540

Similar proteinsi

Entry informationi

Entry nameiMD37A_ARATH
AccessioniPrimary (citable) accession number: Q9LKR3
Secondary accession number(s): Q41928, Q56Y82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
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