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Q9LKR3

- MD37A_ARATH

UniProt

Q9LKR3 - MD37A_ARATH

Protein

Mediator of RNA polymerase II transcription subunit 37a

Gene

MED37A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.1 Publication
    Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Involved in polar nuclei fusion during female gametophyte development and is essential for the regulation of endosperm nuclei proliferation.1 Publication
    In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage By similarity.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. ER-associated ubiquitin-dependent protein catabolic process Source: TAIR
    2. polar nucleus fusion Source: TAIR
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:GQT-238-MONOMER.
    ReactomeiREACT_108190. ATF6-alpha activates chaperone genes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 37a
    Alternative name(s):
    Heat shock 70 kDa protein 11
    Heat shock protein 70-11
    Short name:
    AtHsp70-11
    Luminal-binding protein 1
    Short name:
    AtBP1
    Short name:
    BiP1
    Gene namesi
    Name:MED37A
    Synonyms:BIP1, HSP70-11, MED37_5
    Ordered Locus Names:At5g28540
    ORF Names:T26D3.10, T26D3_50
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G28540.

    Subcellular locationi

    Endoplasmic reticulum lumen PROSITE-ProRule annotation. Nucleus Curated

    GO - Cellular componenti

    1. cell wall Source: TAIR
    2. chloroplast Source: TAIR
    3. cytosol Source: TAIR
    4. endoplasmic reticulum Source: TAIR
    5. endoplasmic reticulum lumen Source: UniProtKB-SubCell
    6. Golgi apparatus Source: TAIR
    7. mediator complex Source: UniProtKB
    8. plasma membrane Source: TAIR
    9. plasmodesma Source: TAIR
    10. vacuolar membrane Source: TAIR
    11. vacuole Source: TAIR

    Keywords - Cellular componenti

    Endoplasmic reticulum, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Bip1 and bip2 double mutation affects the fusion of polar nuclei during female gametophyte development.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 669642Mediator of RNA polymerase II transcription subunit 37aPRO_0000013588Add
    BLAST

    Post-translational modificationi

    Ubiquitinated.1 Publication

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PaxDbiQ9LKR3.
    PRIDEiQ9LKR3.

    2D gel databases

    SWISS-2DPAGEQ9LKR3.

    Expressioni

    Developmental stagei

    Down-regulated during seed maturation. Up-regulated during germination.1 Publication

    Inductioni

    By heat shock.1 Publication

    Gene expression databases

    GenevestigatoriQ9LKR3.

    Interactioni

    Subunit structurei

    Component of the Mediator complex. Interacts with PDIL1-4 and ERDJ3B.4 Publications

    Protein-protein interaction databases

    BioGridi18223. 10 interactions.
    IntActiQ9LKR3. 4 interactions.
    MINTiMINT-7950246.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LKR3.
    SMRiQ9LKR3. Positions 34-652.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi666 – 6694Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0443.
    InParanoidiQ9LKR3.
    KOiK09490.
    OMAiCWIALLF.
    PhylomeDBiQ9LKR3.

    Family and domain databases

    Gene3Di1.20.1270.10. 1 hit.
    2.60.34.10. 1 hit.
    InterProiIPR018181. Heat_shock_70_CS.
    IPR029048. HSP70_C.
    IPR029047. HSP70_peptide-bd.
    IPR013126. Hsp_70_fam.
    [Graphical view]
    PfamiPF00012. HSP70. 1 hit.
    [Graphical view]
    PRINTSiPR00301. HEATSHOCK70.
    SUPFAMiSSF100920. SSF100920. 1 hit.
    SSF100934. SSF100934. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00297. HSP70_1. 1 hit.
    PS00329. HSP70_2. 1 hit.
    PS01036. HSP70_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9LKR3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARSFGANST VVLAIIFFGC LFALSSAIEE ATKLGSVIGI DLGTTYSCVG    50
    VYKNGHVEII ANDQGNRITP SWVGFTDSER LIGEAAKNQA AVNPERTVFD 100
    VKRLIGRKFE DKEVQKDRKL VPYQIVNKDG KPYIQVKIKD GETKVFSPEE 150
    ISAMILTKMK ETAEAYLGKK IKDAVVTVPA YFNDAQRQAT KDAGVIAGLN 200
    VARIINEPTA AAIAYGLDKK GGEKNILVFD LGGGTFDVSV LTIDNGVFEV 250
    LSTNGDTHLG GEDFDHRVME YFIKLIKKKH QKDISKDNKA LGKLRRECER 300
    AKRALSSQHQ VRVEIESLFD GVDFSEPLTR ARFEELNNDL FRKTMGPVKK 350
    AMDDAGLQKS QIDEIVLVGG STRIPKVQQL LKDFFEGKEP NKGVNPDEAV 400
    AYGAAVQGGI LSGEGGDETK DILLLDVAPL TLGIETVGGV MTKLIPRNTV 450
    IPTKKSQVFT TYQDQQTTVS IQVFEGERSL TKDCRLLGKF DLNGIPPAPR 500
    GTPQIEVTFE VDANGILNVK AEDKASGKSE KITITNEKGR LSQEEIDRMV 550
    KEAEEFAEED KKVKEKIDAR NALETYVYNM KNQVNDKDKL ADKLEGDEKE 600
    KIEAATKEAL EWLDENQNSE KEEYDEKLKE VEAVCNPIIT AVYQRSGGAP 650
    GGAGGESSTE EEDESHDEL 669
    Length:669
    Mass (Da):73,629
    Last modified:October 1, 2000 - v1
    Checksum:i20F38DA029F3F3F2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti215 – 2162YG → NV in BAA13947. (PubMed:11130714)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D89341 Genomic DNA. Translation: BAA13947.1.
    AF262043 Genomic DNA. Translation: AAF88019.1.
    CP002688 Genomic DNA. Translation: AED93812.1.
    BT000453 mRNA. Translation: AAN17430.1.
    BT001212 mRNA. Translation: AAN65099.1.
    AK221441 mRNA. Translation: BAD94482.1.
    Z17760 mRNA. Translation: CAA79056.1.
    RefSeqiNP_198206.1. NM_122737.3.
    UniGeneiAt.23381.
    At.49118.
    At.49188.
    At.64764.

    Genome annotation databases

    EnsemblPlantsiAT5G28540.1; AT5G28540.1; AT5G28540.
    GeneIDi832950.
    KEGGiath:AT5G28540.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D89341 Genomic DNA. Translation: BAA13947.1 .
    AF262043 Genomic DNA. Translation: AAF88019.1 .
    CP002688 Genomic DNA. Translation: AED93812.1 .
    BT000453 mRNA. Translation: AAN17430.1 .
    BT001212 mRNA. Translation: AAN65099.1 .
    AK221441 mRNA. Translation: BAD94482.1 .
    Z17760 mRNA. Translation: CAA79056.1 .
    RefSeqi NP_198206.1. NM_122737.3.
    UniGenei At.23381.
    At.49118.
    At.49188.
    At.64764.

    3D structure databases

    ProteinModelPortali Q9LKR3.
    SMRi Q9LKR3. Positions 34-652.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 18223. 10 interactions.
    IntActi Q9LKR3. 4 interactions.
    MINTi MINT-7950246.

    2D gel databases

    SWISS-2DPAGE Q9LKR3.

    Proteomic databases

    PaxDbi Q9LKR3.
    PRIDEi Q9LKR3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G28540.1 ; AT5G28540.1 ; AT5G28540 .
    GeneIDi 832950.
    KEGGi ath:AT5G28540.

    Organism-specific databases

    TAIRi AT5G28540.

    Phylogenomic databases

    eggNOGi COG0443.
    InParanoidi Q9LKR3.
    KOi K09490.
    OMAi CWIALLF.
    PhylomeDBi Q9LKR3.

    Enzyme and pathway databases

    BioCyci ARA:GQT-238-MONOMER.
    Reactomei REACT_108190. ATF6-alpha activates chaperone genes.

    Gene expression databases

    Genevestigatori Q9LKR3.

    Family and domain databases

    Gene3Di 1.20.1270.10. 1 hit.
    2.60.34.10. 1 hit.
    InterProi IPR018181. Heat_shock_70_CS.
    IPR029048. HSP70_C.
    IPR029047. HSP70_peptide-bd.
    IPR013126. Hsp_70_fam.
    [Graphical view ]
    Pfami PF00012. HSP70. 1 hit.
    [Graphical view ]
    PRINTSi PR00301. HEATSHOCK70.
    SUPFAMi SSF100920. SSF100920. 1 hit.
    SSF100934. SSF100934. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00297. HSP70_1. 1 hit.
    PS00329. HSP70_2. 1 hit.
    PS01036. HSP70_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of two genes encoding luminal binding protein from Arabidopsis thaliana."
      Koizumi N., Sano H.
      Plant Gene Register PGR97-028
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 537-669.
      Strain: cv. Columbia.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 620-669.
      Strain: cv. Columbia.
      Tissue: Green siliques.
    7. "Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana."
      Lin B.L., Wang J.S., Liu H.C., Chen R.W., Meyer Y., Barakat A., Delseny M.
      Cell Stress Chaperones 6:201-208(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    8. "Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family."
      Sung D.Y., Vierling E., Guy C.L.
      Plant Physiol. 126:789-800(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNAK GENE SUBFAMILY, INDUCTION, DEVELOPMENTAL STAGE.
    9. "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1 as the Med25 subunit."
      Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.
      Mol. Cell 26:717-729(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, NOMENCLATURE.
    10. Cited for: INTERACTION WITH ERDJ3B.
    11. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
      Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
      Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
    12. "BiP-mediated polar nuclei fusion is essential for the regulation of endosperm nuclei proliferation in Arabidopsis thaliana."
      Maruyama D., Endo T., Nishikawa S.
      Proc. Natl. Acad. Sci. U.S.A. 107:1684-1689(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    13. "Protein disulfide isomerase-2 of Arabidopsis mediates protein folding and localizes to both the secretory pathway and nucleus, where it interacts with maternal effect embryo arrest factor."
      Cho E.J., Yuen C.Y., Kang B.H., Ondzighi C.A., Staehelin L.A., Christopher D.A.
      Mol. Cells 32:459-475(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PDIL1-4.
    14. "The Mediator complex in plants: structure, phylogeny, and expression profiling of representative genes in a dicot (Arabidopsis) and a monocot (rice) during reproduction and abiotic stress."
      Mathur S., Vyas S., Kapoor S., Tyagi A.K.
      Plant Physiol. 157:1609-1627(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, SUBUNIT, NOMENCLATURE.

    Entry informationi

    Entry nameiMD37A_ARATH
    AccessioniPrimary (citable) accession number: Q9LKR3
    Secondary accession number(s): Q41928, Q56Y82
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3