Q9LKR3 (MD37A_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mediator of RNA polymerase II transcription subunit 37a

Alternative name(s):
Heat shock 70 kDa protein 11
Heat shock protein 70-11
Short name=AtHsp70-11
Luminal-binding protein 1
Short name=AtBP1
Short name=BiP1

Gene names

Name:	MED37A
Synonyms:	BIP1, HSP70-11, MED37_5
Ordered Locus Names:	At5g28540
ORF Names:	T26D3.10, T26D3_50

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	669 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Ref.11 Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Involved in polar nuclei fusion during female gametophyte development and is essential for the regulation of endosperm nuclei proliferation. Ref.11 In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage By similarity. Ref.11
Subunit structure	Component of the Mediator complex. Interacts with PDIL1-4. Ref.9 Ref.12 Ref.13
Subcellular location	Endoplasmic reticulum lumen By similarity. Nucleus Probable.
Developmental stage	Down-regulated during seed maturation. Up-regulated during germination. Ref.8
Induction	By heat shock. Ref.8
Post-translational modification	Ubiquitinated.
Disruption phenotype	bip1 bip2 double mutation affects the fusion of polar nuclei during female gametophyte development. Ref.11
Sequence similarities	Belongs to the heat shock protein 70 (TC 1.A.33) family. DnaK subfamily. [View classification]

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Endoplasmic reticulum Nucleus
Domain	Signal
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER-associated protein catabolic process Inferred from mutant phenotype PubMed 19060110. Source: TAIR polar nucleus fusion Inferred from genetic interaction Ref.11. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 22430844. Source: TAIR cell wall Inferred from direct assay PubMed 16287169. Source: TAIR chloroplast Inferred from direct assay PubMed 15028209PubMed 18431481. Source: TAIR cytosol Inferred from direct assay PubMed 21166475. Source: TAIR endoplasmic reticulum Inferred from direct assay PubMed 22923678. Source: TAIR endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell mediator complex Inferred from direct assay Ref.9. Source: UniProtKB plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR vacuolar membrane Inferred from direct assay PubMed 17151019. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 669	642	Mediator of RNA polymerase II transcription subunit 37a		PRO_0000013588
Regions
	Motif	666 – 669	4	Prevents secretion from ER Potential
Experimental info
	Sequence conflict	215 – 216	2	YG → NV in BAA13947. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9LKR3 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 20F38DA029F3F3F2	FASTA	669	73,629
	10 20 30 40 50 60 MARSFGANST VVLAIIFFGC LFALSSAIEE ATKLGSVIGI DLGTTYSCVG VYKNGHVEII 70 80 90 100 110 120 ANDQGNRITP SWVGFTDSER LIGEAAKNQA AVNPERTVFD VKRLIGRKFE DKEVQKDRKL 130 140 150 160 170 180 VPYQIVNKDG KPYIQVKIKD GETKVFSPEE ISAMILTKMK ETAEAYLGKK IKDAVVTVPA 190 200 210 220 230 240 YFNDAQRQAT KDAGVIAGLN VARIINEPTA AAIAYGLDKK GGEKNILVFD LGGGTFDVSV 250 260 270 280 290 300 LTIDNGVFEV LSTNGDTHLG GEDFDHRVME YFIKLIKKKH QKDISKDNKA LGKLRRECER 310 320 330 340 350 360 AKRALSSQHQ VRVEIESLFD GVDFSEPLTR ARFEELNNDL FRKTMGPVKK AMDDAGLQKS 370 380 390 400 410 420 QIDEIVLVGG STRIPKVQQL LKDFFEGKEP NKGVNPDEAV AYGAAVQGGI LSGEGGDETK 430 440 450 460 470 480 DILLLDVAPL TLGIETVGGV MTKLIPRNTV IPTKKSQVFT TYQDQQTTVS IQVFEGERSL 490 500 510 520 530 540 TKDCRLLGKF DLNGIPPAPR GTPQIEVTFE VDANGILNVK AEDKASGKSE KITITNEKGR 550 560 570 580 590 600 LSQEEIDRMV KEAEEFAEED KKVKEKIDAR NALETYVYNM KNQVNDKDKL ADKLEGDEKE 610 620 630 640 650 660 KIEAATKEAL EWLDENQNSE KEEYDEKLKE VEAVCNPIIT AVYQRSGGAP GGAGGESSTE EEDESHDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of two genes encoding luminal binding protein from Arabidopsis thaliana." Koizumi N., Sano H. Plant Gene Register PGR97-028 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 537-669. Strain: cv. Columbia.
[6]	"An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana." Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M., Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D., Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M., Cooke R. , Grellet F., Delseny M., Parmentier Y., de Marcillac G., Gigot C., Fleck J., Philipps G., Axelos M., Bardet C., Tremousaygue D., Lescure B. Plant J. 4:1051-1061(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 620-669. Strain: cv. Columbia. Tissue: Green siliques.
[7]	"Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana." Lin B.L., Wang J.S., Liu H.C., Chen R.W., Meyer Y., Barakat A., Delseny M. Cell Stress Chaperones 6:201-208(2001) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[8]	"Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family." Sung D.Y., Vierling E., Guy C.L. Plant Physiol. 126:789-800(2001) [PubMed] [Europe PMC] [Abstract] Cited for: DNAK GENE SUBFAMILY, INDUCTION, DEVELOPMENTAL STAGE.
[9]	"Purification of a plant mediator from Arabidopsis thaliana identifies PFT1 as the Med25 subunit." Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S. Mol. Cell 26:717-729(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, NOMENCLATURE.
[10]	"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis." Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D. Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
[11]	"BiP-mediated polar nuclei fusion is essential for the regulation of endosperm nuclei proliferation in Arabidopsis thaliana." Maruyama D., Endo T., Nishikawa S. Proc. Natl. Acad. Sci. U.S.A. 107:1684-1689(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[12]	"Protein disulfide isomerase-2 of Arabidopsis mediates protein folding and localizes to both the secretory pathway and nucleus, where it interacts with maternal effect embryo arrest factor." Cho E.J., Yuen C.Y., Kang B.H., Ondzighi C.A., Staehelin L.A., Christopher D.A. Mol. Cells 32:459-475(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PDIL1-4.
[13]	"The Mediator complex in plants: structure, phylogeny, and expression profiling of representative genes in a dicot (Arabidopsis) and a monocot (rice) during reproduction and abiotic stress." Mathur S., Vyas S., Kapoor S., Tyagi A.K. Plant Physiol. 157:1609-1627(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, SUBUNIT, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D89341 Genomic DNA. Translation: BAA13947.1. AF262043 Genomic DNA. Translation: AAF88019.1. CP002688 Genomic DNA. Translation: AED93812.1. BT000453 mRNA. Translation: AAN17430.1. BT001212 mRNA. Translation: AAN65099.1. AK221441 mRNA. Translation: BAD94482.1. Z17760 mRNA. Translation: CAA79056.1.
IPI	IPI00522684.
RefSeq	NP_198206.1. NM_122737.3.
UniGene	At.23381. At.49118. At.49188. At.64764.
3D structure databases
ProteinModelPortal	Q9LKR3.
SMR	Q9LKR3. Positions 34-652.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LKR3. 1 interaction.
2D gel databases
SWISS-2DPAGE	Q9LKR3.
Proteomic databases
PaxDb	Q9LKR3.
PRIDE	Q9LKR3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G28540.1; AT5G28540.1; AT5G28540.
GeneID	832950.
KEGG	ath:AT5G28540.
Organism-specific databases
TAIR	At5g28540.
Phylogenomic databases
eggNOG	COG0443.
InParanoid	Q9LKR3.
KO	K09490.
OMA	LINRNTA.
PhylomeDB	Q9LKR3.
ProtClustDB	CLSN2713985.
Gene expression databases
ArrayExpress	Q9LKR3.
Genevestigator	Q9LKR3.
GermOnline	AT5G28540. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR018181. Heat_shock_70_CS. IPR013126. Hsp_70_fam. [Graphical view]
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MD37A_ARATH

Accession

Primary (citable) accession number: Q9LKR3
Secondary accession number(s): Q41928, Q56Y82

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program