Reviewed, UniProtKB/Swiss-Prot Q9LKN0 (CHMO_ATRHO)

Names and origin

Protein names

Recommended name:
    Choline monooxygenase, chloroplastic
    EC=1.14.15.7

Gene names

Name:

CMO

Organism

Atriplex hortensis (Mountain spinach)

Taxonomic identifier

34272 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Atriplex

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the first step of the osmoprotectant glycine betaine synthesis.
Catalytic activity	Choline + O2 + 2 reduced ferredoxin + 2 H+ = betaine aldehyde hydrate + H2O + 2 oxidized ferredoxin.
Cofactor	Binds 1 2Fe-2S cluster. Binds 1 iron ion Probable. Magnesium By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (monooxygenase route): step 1/1.
Subcellular location	Plastid › chloroplast stroma By similarity.
Tissue specificity	Expressed in roots and leaves.
Induction	By salt stress.
Sequence similarities	Belongs to the choline monooxygenase family. Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	2Fe-2S Iron Iron-sulfur Magnesium Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	cellular aromatic compound metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW choline monooxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 58	58	Chloroplast By similarity
	Chain	59 – 438	380	Choline monooxygenase, chloroplastic		PRO_0000020926
Regions
	Domain	121 – 228	108	Rieske
	Compositional bias	69 – 75	7	Poly-Thr
Sites
	Metal binding	163	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	165	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	182	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	185	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	288	1	Iron Potential
	Metal binding	293	1	Iron Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9LKN0-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C63970A1DD1CADE3	FASTA	438	48,771
	10 20 30 40 50 60 MAASATTMLL KYPTTVCGIP NSSANNSTDP SNNIVQIPQT TTTNSPLLKF RTPNKPVNAV 70 80 90 100 110 120 AAPAFPSVTT TTTTTPSSIQ SLVKDFDPLV PAEDALTPPS SWYTEPAFYA HELDRIFYKG 130 140 150 160 170 180 WQVAGYSDQV KEANQYFTGT LGNVEYLVCR DGEGKVHAFH NVCTHRASIL ACGSGKKSCF 190 200 210 220 230 240 VCPYHGWVYG MNGSLTKASK ATPEQSLNPD ELGLVPLKVA VWGPFILISL DRSSREVGDV 250 260 270 280 290 300 GSEWLGSCAE DVKAHAFDPN LQFINRSEFP IESNWKIFSD NYLDSSYHVP YAHKYYATEL 310 320 330 340 350 360 DFDTYQTDMV GNVTIQRVAG TSNNGFNRLG TQAFYAFAYP NFAVERYGPW MTTMHIVPLG 370 380 390 400 410 420 PRKCKLVVDY YIEKSKLDDK DYIEKGIAIN DNVQKEDVVL CESVQKGLET PAYRSGRYVM 430 PIEKGIHHFH CWLHQVLK

References

[1]	"Cloning and characterization of CMO gene from Atriplex hortensis." Shen Y.G., Du B.X., Zhang J.S., Chen S.Y. Sheng Wu Gong Cheng Xue Bao 17:1-6(2001) [PubMed: 11330176] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF270651 mRNA. Translation: AAF76895.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.15.7. 305150.
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR001663. Rng_hydr_dOase-A. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR21266:SF2. Rng_hydr_dOase-A. 1 hit.
Pfam	PF00355. Rieske. 1 hit. [Graphical view]
PRINTS	PR00090. RNGDIOXGNASE.
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHMO_ATRHO

Accession

Primary (citable) accession number: Q9LKN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)