Two-component response regulator-like APRR1 - Arabidopsis thaliana (Mouse-ear cress)

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Reviewed, UniProtKB/Swiss-Prot Q9LKL2 (APRR1_ARATH)

Last modified June 16, 2009. Version 65. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Two-component response regulator-like APRR1
Alternative name(s):
    Pseudo-response regulator 1
    Timing of CAB expression 1
    ABI3-interacting protein 1

Gene names

Name:	APRR1
Synonyms:	AIP1, TOC1
Ordered Locus Names:	At5g61380
ORF Names:	MFB13.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Controls photoperiodic flowering response. Seems to be one of the component of the circadian clock. Expression of several members of the ARR-like family is controlled by circadian rhythm. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock. Ref.6
Subunit structure	Interacts with PIF1, PIL2, PIF3, PIF4, PIL5, PIL6, ABI3, ADO1 and ADO2. Ref.3 Ref.8 Ref.9 Ref.10
Subcellular location	Nucleus.
Tissue specificity	Expressed in leaves, flowers and siliques. Ref.3
Induction	Expressed with a circadian rhythm showing a broad peak in the late day. Negatively regulated by LHY and CCA1. Ref.7
Sequence similarities	Belongs to the ARR-like family. Contains 1 CCT domain. Contains 1 response regulatory domain.
Caution	Lacks the phospho-accepting Asp (here Glu-71), present in the receiver domain, which is one of the conserved features of the two-component response regulators (ARRs) family.

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Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Nucleus
Domain	Coiled coil
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	circadian rhythm Ref.2 Inferred from expression pattern. Source: TAIR circumnutation Inferred from mutant phenotype. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.2 Inferred from direct assay. Source: TAIR
Molecular function	protein binding Ref.3 Inferred from physical interaction. Source: IntAct two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
ABI3	Q01593	2	EBI-618423,EBI-1578892
ADO1	Q94BT6	4	EBI-618423,EBI-300691
ADO2	Q8W420	2	EBI-618423,EBI-1015688
APRR3	Q9LVG4	1	EBI-618423,EBI-1606968
PIL1	Q8L5W8	3	EBI-618423,EBI-630752

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 618

618

Two-component response regulator-like APRR1

PRO_0000081435

Regions

Domain

20 – 138

119

Response regulatory

Domain

533 – 575

CCT

Coiled coil

588 – 610

Potential

Compositional bias

595 – 605

Poly-Glu

Experimental info

Mutagenesis

562

A → V in toc1-1; causes shortened circadian rhythms in light-grown plants. Ref.2

Sequence conflict

126

R → L in CAB75508. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9LKL2-1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: EDBE0AA0B7FAB61E
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FASTA

618

69,195

        10         20         30         40         50         60 
MDLNGECKGG DGFIDRSRVR ILLCDNDSTS LGEVFTLLSE CSYQVTAVKS ARQVIDALNA 

        70         80         90        100        110        120 
EGPDIDIILA EIDLPMAKGM KMLRYITRDK DLRRIPVIMM SRQDEVPVVV KCLKLGAADY 

       130        140        150        160        170        180 
LVKPLRTNEL LNLWTHMWRR RRMLGLAEKN MLSYDFDLVG SDQSDPNTNS TNLFSDDTDD 

       190        200        210        220        230        240 
RSLRSTNPQR GNLSHQENEW SVATAPVHAR DGGLGADGTA TSSLAVTAIE PPLDHLAGSH 

       250        260        270        280        290        300 
HEPMKRNSNP AQFSSAPKKS RLKIGESSAF FTYVKSTVLR TNGQDPPLVD GNGSLHLHRG 

       310        320        330        340        350        360 
LAEKFQVVAS EGINNTKQAR RATPKSTVLR TNGQDPPLVN GNGSHHLHRG AAEKFQVVAS 

       370        380        390        400        410        420 
EGINNTKQAH RSRGTEQYHS QGETLQNGAS YPHSLERSRT LPTSMESHGR NYQEGNMNIP 

       430        440        450        460        470        480 
QVAMNRSKDS SQVDGSGFSA PNAYPYYMHG VMNQVMMQSA AMMPQYGHQI PHCQPNHPNG 

       490        500        510        520        530        540 
MTGYPYYHHP MNTSLQHSQM SLQNGQMSMV HHSWSPAGNP PSNEVRVNKL DRREEALLKF 

       550        560        570        580        590        600 
RRKRNQRCFD KKIRYVNRKR LAERRPRVKG QFVRKMNGVN VDLNGQPDSA DYDDEEEEEE 

       610 
EEEEENRDSS PQDDALGT

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genes encoding pseudo-response regulators: insight into His-to-Asp phosphorelay and circadian rhythm in Arabidopsis thaliana." Makino S., Kiba T., Imamura A., Hanaki N., Nakamura A., Suzuki T., Taniguchi M., Ueguchi C., Sugiyama T., Mizuno T. Plant Cell Physiol. 41:791-803(2000) [PubMed: 10945350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, CHARACTERIZATION, GENE FAMILY. Strain: cv. Columbia.
[2]	"Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog." Strayer C., Oyama T., Schultz T.F., Raman R., Somers D.E., Mas P., Panda S., Kreps J.A., Kay S.A. Science 289:768-771(2000) [PubMed: 10926537] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF ALA-562. Strain: cv. Columbia.
[3]	"Interactions of the developmental regulator ABI3 with proteins identified from developing Arabidopsis seeds." Kurup S., Jones H.D., Holdsworth M.J. Plant J. 21:143-155(2000) [PubMed: 10743655] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH ABI3. Strain: cv. Landsberg erecta.
[4]	"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones." Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:41-54(1998) [PubMed: 9628582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Circadian waves of expression of the APRR1/TOC1 family of pseudo-response regulators in Arabidopsis thaliana: insight into the plant circadian clock." Matsushika A., Makino S., Kojima M., Mizuno T. Plant Cell Physiol. 41:1002-1012(2000) [PubMed: 11100772] [Abstract] Cited for: FUNCTION.
[7]	"Reciprocal regulation between TOC1 and LHY/CCA1 within the Arabidopsis circadian clock." Alabadi D., Oyama T., Yanovsky M.J., Harmon F.G., Mas P., Kay S.A. Science 293:880-883(2001) [PubMed: 11486091] [Abstract] Cited for: INDUCTION.
[8]	"The APRR1/TOC1 quintet implicated in circadian rhythms of Arabidopsis thaliana: I. Characterization with APRR1-overexpressing plants." Makino S., Matsushika A., Kojima M., Yamashino T., Mizuno T. Plant Cell Physiol. 43:58-69(2002) [PubMed: 11828023] [Abstract] Cited for: INTERACTION WITH PIF1.
[9]	"A Link between circadian-controlled bHLH factors and the APRR1/TOC1 quintet in Arabidopsis thaliana." Yamashino T., Matsushika A., Fujimori T., Sato S., Kato T., Tabata S., Mizuno T. Plant Cell Physiol. 44:619-629(2003) [PubMed: 12826627] [Abstract] Cited for: INTERACTION WITH PIF1; PIL2; PIF3; PIF4; PIL5 AND PIL6.
[10]	"Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis." Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T. J. Exp. Bot. 55:2015-2027(2004) [PubMed: 15310821] [Abstract] Cited for: INTERACTION WITH ADO1 AND ADO2.

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Cross-references

Sequence databases
	AB041530 mRNA. Translation: BAA94547.1. AF272039 mRNA. Translation: AAF86252.1. AJ251086 mRNA. Translation: CAB75508.1. AB010073 Genomic DNA. Translation: BAB08493.1. AY094393 mRNA. Translation: AAM19772.1. BT005816 mRNA. Translation: AAO64751.1.
IPI	IPI00517214.
PIR	T52075. T52076.
RefSeq	NP_200946.1.
UniGene	At.21919
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LKL2. 6 interactions.
Genome annotation databases
GeneID	836259.
GenomeReviews	Gene locus AT5G61380 in contig BA000015_GR.
KEGG	ath:AT5G61380.
NMPDR	fig\|3702.1.peg.28159.
Organism-specific databases
GeneFarm	4313. 437.
TAIR	At5g61380.
Phylogenomic databases
OMA	Q9LKL2. LLNLWTH.
Gene expression databases
ArrayExpress	Q9LKL2.
GermOnline	AT5G61380. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR010402. CCT_domain. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Pfam	PF06203. CCT. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
ProDom	PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS51017. CCT. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

APRR1_ARATH

Accession

Primary (citable) accession number: Q9LKL2
Secondary accession number(s): Q9M4B7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents