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Q9LKL2

- APRR1_ARATH

UniProt

Q9LKL2 - APRR1_ARATH

Protein

Two-component response regulator-like APRR1

Gene

APRR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Controls photoperiodic flowering response. Component of the circadian clock. Expression of several members of the ARR-like family is controlled by circadian rhythm. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock. Positive regulator of CCA1 and LHY expression.2 Publications

    GO - Molecular functioni

    1. DNA binding Source: TAIR
    2. phosphorelay response regulator activity Source: InterPro
    3. protein binding Source: UniProtKB
    4. sequence-specific DNA binding transcription factor activity Source: TAIR

    GO - Biological processi

    1. circadian rhythm Source: TAIR
    2. circumnutation Source: TAIR
    3. flower development Source: UniProtKB-KW
    4. negative regulation of gene expression Source: TAIR
    5. regulation of transcription, DNA-templated Source: TAIR
    6. transcription, DNA-templated Source: UniProtKB

    Keywords - Biological processi

    Biological rhythms, Flowering, Transcription, Transcription regulation, Two-component regulatory system

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Two-component response regulator-like APRR1
    Alternative name(s):
    ABI3-interacting protein 1
    Pseudo-response regulator 1
    Timing of CAB expression 1
    Gene namesi
    Name:APRR1
    Synonyms:AIP1, TOC1
    Ordered Locus Names:At5g61380
    ORF Names:MFB13.16
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G61380.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi562 – 5621A → V in toc1-1; causes shortened circadian rhythms in light-grown plants. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 618618Two-component response regulator-like APRR1PRO_0000081435Add
    BLAST

    Post-translational modificationi

    Phosphorylated; during the day. Phosphorylation is required for optimal interaction with APRR3.1 Publication

    Proteomic databases

    PRIDEiQ9LKL2.

    Expressioni

    Tissue specificityi

    Expressed in leaves, flowers and siliques. Restricted to the vasculature.2 Publications

    Inductioni

    Expressed with a circadian rhythm showing a broad peak in the late day and early night. Negatively regulated by LHY and CCA1.2 Publications

    Gene expression databases

    GenevestigatoriQ9LKL2.

    Interactioni

    Subunit structurei

    Interacts with PIF1, PIL2, PIF3, PIF4, PIL5, PIL6, ABI3 (via C-terminus), ADO1/ZTL, ADO2, APRR3 and TCP21/CHE. Both the phosphorylated and the dephosphorylated forms interact with ADO1/ZLT.8 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ADO1Q94BT67EBI-618423,EBI-300691
    APRR3Q9LVG43EBI-618423,EBI-1606968
    APRR5Q6LA425EBI-618423,EBI-1536669
    PIL1Q8L5W83EBI-618423,EBI-630752

    Protein-protein interaction databases

    BioGridi21503. 29 interactions.
    DIPiDIP-33896N.
    IntActiQ9LKL2. 19 interactions.
    MINTiMINT-7908647.
    STRINGi3702.AT5G61380.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LKL2.
    SMRiQ9LKL2. Positions 20-131.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 138119Response regulatoryPROSITE-ProRule annotationAdd
    BLAST
    Domaini533 – 57543CCTPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili588 – 61023Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi595 – 60511Poly-GluAdd
    BLAST

    Domaini

    The N-terminus (1-243) is required for interactions with ADO1/ZTL and APRR3.

    Sequence similaritiesi

    Belongs to the ARR-like family.Curated
    Contains 1 CCT domain.PROSITE-ProRule annotation
    Contains 1 response regulatory domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG242953.
    HOGENOMiHOG000034017.
    InParanoidiQ9LKL2.
    KOiK12127.
    OMAiFVRQASN.
    PhylomeDBiQ9LKL2.

    Family and domain databases

    InterProiIPR010402. CCT_domain.
    IPR011006. CheY-like_superfamily.
    IPR001789. Sig_transdc_resp-reg_receiver.
    [Graphical view]
    PfamiPF06203. CCT. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view]
    SMARTiSM00448. REC. 1 hit.
    [Graphical view]
    SUPFAMiSSF52172. SSF52172. 1 hit.
    PROSITEiPS51017. CCT. 1 hit.
    PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9LKL2-1 [UniParc]FASTAAdd to Basket

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    MDLNGECKGG DGFIDRSRVR ILLCDNDSTS LGEVFTLLSE CSYQVTAVKS    50
    ARQVIDALNA EGPDIDIILA EIDLPMAKGM KMLRYITRDK DLRRIPVIMM 100
    SRQDEVPVVV KCLKLGAADY LVKPLRTNEL LNLWTHMWRR RRMLGLAEKN 150
    MLSYDFDLVG SDQSDPNTNS TNLFSDDTDD RSLRSTNPQR GNLSHQENEW 200
    SVATAPVHAR DGGLGADGTA TSSLAVTAIE PPLDHLAGSH HEPMKRNSNP 250
    AQFSSAPKKS RLKIGESSAF FTYVKSTVLR TNGQDPPLVD GNGSLHLHRG 300
    LAEKFQVVAS EGINNTKQAR RATPKSTVLR TNGQDPPLVN GNGSHHLHRG 350
    AAEKFQVVAS EGINNTKQAH RSRGTEQYHS QGETLQNGAS YPHSLERSRT 400
    LPTSMESHGR NYQEGNMNIP QVAMNRSKDS SQVDGSGFSA PNAYPYYMHG 450
    VMNQVMMQSA AMMPQYGHQI PHCQPNHPNG MTGYPYYHHP MNTSLQHSQM 500
    SLQNGQMSMV HHSWSPAGNP PSNEVRVNKL DRREEALLKF RRKRNQRCFD 550
    KKIRYVNRKR LAERRPRVKG QFVRKMNGVN VDLNGQPDSA DYDDEEEEEE 600
    EEEEENRDSS PQDDALGT 618
    Length:618
    Mass (Da):69,195
    Last modified:October 1, 2000 - v1
    Checksum:iEDBE0AA0B7FAB61E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti126 – 1261R → L in CAB75508. (PubMed:10926537)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB041530 mRNA. Translation: BAA94547.1.
    AF272039 mRNA. Translation: AAF86252.1.
    AJ251086 mRNA. Translation: CAB75508.1.
    AB010073 Genomic DNA. Translation: BAB08493.1.
    CP002688 Genomic DNA. Translation: AED97460.1.
    AY094393 mRNA. Translation: AAM19772.1.
    BT005816 mRNA. Translation: AAO64751.1.
    PIRiT52075.
    T52076.
    RefSeqiNP_200946.1. NM_125531.3.
    UniGeneiAt.21919.

    Genome annotation databases

    EnsemblPlantsiAT5G61380.1; AT5G61380.1; AT5G61380.
    GeneIDi836259.
    KEGGiath:AT5G61380.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB041530 mRNA. Translation: BAA94547.1 .
    AF272039 mRNA. Translation: AAF86252.1 .
    AJ251086 mRNA. Translation: CAB75508.1 .
    AB010073 Genomic DNA. Translation: BAB08493.1 .
    CP002688 Genomic DNA. Translation: AED97460.1 .
    AY094393 mRNA. Translation: AAM19772.1 .
    BT005816 mRNA. Translation: AAO64751.1 .
    PIRi T52075.
    T52076.
    RefSeqi NP_200946.1. NM_125531.3.
    UniGenei At.21919.

    3D structure databases

    ProteinModelPortali Q9LKL2.
    SMRi Q9LKL2. Positions 20-131.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 21503. 29 interactions.
    DIPi DIP-33896N.
    IntActi Q9LKL2. 19 interactions.
    MINTi MINT-7908647.
    STRINGi 3702.AT5G61380.1-P.

    Proteomic databases

    PRIDEi Q9LKL2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G61380.1 ; AT5G61380.1 ; AT5G61380 .
    GeneIDi 836259.
    KEGGi ath:AT5G61380.

    Organism-specific databases

    GeneFarmi 4313. 437.
    TAIRi AT5G61380.

    Phylogenomic databases

    eggNOGi NOG242953.
    HOGENOMi HOG000034017.
    InParanoidi Q9LKL2.
    KOi K12127.
    OMAi FVRQASN.
    PhylomeDBi Q9LKL2.

    Gene expression databases

    Genevestigatori Q9LKL2.

    Family and domain databases

    InterProi IPR010402. CCT_domain.
    IPR011006. CheY-like_superfamily.
    IPR001789. Sig_transdc_resp-reg_receiver.
    [Graphical view ]
    Pfami PF06203. CCT. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view ]
    SMARTi SM00448. REC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52172. SSF52172. 1 hit.
    PROSITEi PS51017. CCT. 1 hit.
    PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genes encoding pseudo-response regulators: insight into His-to-Asp phosphorelay and circadian rhythm in Arabidopsis thaliana."
      Makino S., Kiba T., Imamura A., Hanaki N., Nakamura A., Suzuki T., Taniguchi M., Ueguchi C., Sugiyama T., Mizuno T.
      Plant Cell Physiol. 41:791-803(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, GENE FAMILY.
      Strain: cv. Columbia.
    2. "Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog."
      Strayer C., Oyama T., Schultz T.F., Raman R., Somers D.E., Mas P., Panda S., Kreps J.A., Kay S.A.
      Science 289:768-771(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF ALA-562.
      Strain: cv. Columbia.
    3. "Interactions of the developmental regulator ABI3 with proteins identified from developing Arabidopsis seeds."
      Kurup S., Jones H.D., Holdsworth M.J.
      Plant J. 21:143-155(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH ABI3.
      Strain: cv. Landsberg erecta.
    4. "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
      Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    7. "Circadian waves of expression of the APRR1/TOC1 family of pseudo-response regulators in Arabidopsis thaliana: insight into the plant circadian clock."
      Matsushika A., Makino S., Kojima M., Mizuno T.
      Plant Cell Physiol. 41:1002-1012(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Reciprocal regulation between TOC1 and LHY/CCA1 within the Arabidopsis circadian clock."
      Alabadi D., Oyama T., Yanovsky M.J., Harmon F.G., Mas P., Kay S.A.
      Science 293:880-883(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    9. "The APRR1/TOC1 quintet implicated in circadian rhythms of Arabidopsis thaliana: I. Characterization with APRR1-overexpressing plants."
      Makino S., Matsushika A., Kojima M., Yamashino T., Mizuno T.
      Plant Cell Physiol. 43:58-69(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PIF1.
    10. "A link between circadian-controlled bHLH factors and the APRR1/TOC1 quintet in Arabidopsis thaliana."
      Yamashino T., Matsushika A., Fujimori T., Sato S., Kato T., Tabata S., Mizuno T.
      Plant Cell Physiol. 44:619-629(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PIF1; PIL2; PIF3; PIF4; PIL5 AND PIL6.
    11. "Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis."
      Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T.
      J. Exp. Bot. 55:2015-2027(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ADO1 AND ADO2.
    12. Cited for: INTERACTION WITH ADO1.
    13. "PRR3 Is a vascular regulator of TOC1 stability in the Arabidopsis circadian clock."
      Para A., Farre E.M., Imaizumi T., Pruneda-Paz J.L., Harmon F.G., Kay S.A.
      Plant Cell 19:3462-3473(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INTERACTION WITH APRR3.
    14. "Post-translational regulation of the Arabidopsis circadian clock through selective proteolysis and phosphorylation of pseudo-response regulator proteins."
      Fujiwara S., Wang L., Han L., Suh S.-S., Salome P.A., McClung C.R., Somers D.E.
      J. Biol. Chem. 283:23073-23083(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION, INDUCTION, SUBCELLULAR LOCATION, INTERACTION WITH ADO1 AND APRR3.
    15. "CUL1 regulates TOC1 protein stability in the Arabidopsis circadian clock."
      Harmon F., Imaizumi T., Gray W.M.
      Plant J. 55:568-579(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.
    16. "A functional genomics approach reveals CHE as a component of the Arabidopsis circadian clock."
      Pruneda-Paz J.L., Breton G., Para A., Kay S.A.
      Science 323:1481-1485(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TCP21.

    Entry informationi

    Entry nameiAPRR1_ARATH
    AccessioniPrimary (citable) accession number: Q9LKL2
    Secondary accession number(s): Q9M4B7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2004
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Subject of targeted degradation by the 26S proteasome. ZEITLUPE (ADO1/ZTL) is the F-box protein that associates with the SCF (for Skp/Cullin/F-box) E3 ubiquitin ligase that is responsible for marking APRR1/TOC1 for turnover. CUL1 is the functional cullin for the SCF(ZTL) complex. APRR3 binding competitively inhibits the ADO1/ZTL interaction.

    Caution

    Lacks the phospho-accepting Asp (here Glu-71), present in the receiver domain, which is one of the conserved features of the two-component response regulators (ARRs) family.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3