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Protein

Two-component response regulator-like APRR1

Gene

APRR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Controls photoperiodic flowering response. Component of the circadian clock. Expression of several members of the ARR-like family is controlled by circadian rhythm. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock. Positive regulator of CCA1 and LHY expression.2 Publications

GO - Molecular functioni

  1. DNA binding Source: TAIR
  2. sequence-specific DNA binding transcription factor activity Source: TAIR

GO - Biological processi

  1. circadian rhythm Source: TAIR
  2. circumnutation Source: TAIR
  3. flower development Source: UniProtKB-KW
  4. negative regulation of gene expression Source: TAIR
  5. phosphorelay signal transduction system Source: UniProtKB-KW
  6. regulation of transcription, DNA-templated Source: TAIR
  7. transcription, DNA-templated Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Biological rhythms, Flowering, Transcription, Transcription regulation, Two-component regulatory system

Names & Taxonomyi

Protein namesi
Recommended name:
Two-component response regulator-like APRR1
Alternative name(s):
ABI3-interacting protein 1
Pseudo-response regulator 1
Timing of CAB expression 1
Gene namesi
Name:APRR1
Synonyms:AIP1, TOC1
Ordered Locus Names:At5g61380
ORF Names:MFB13.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G61380.

Subcellular locationi

Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi562 – 5621A → V in toc1-1; causes shortened circadian rhythms in light-grown plants. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 618618Two-component response regulator-like APRR1PRO_0000081435Add
BLAST

Post-translational modificationi

Phosphorylated; during the day. Phosphorylation is required for optimal interaction with APRR3.1 Publication

Proteomic databases

PRIDEiQ9LKL2.

Expressioni

Tissue specificityi

Expressed in leaves, flowers and siliques. Restricted to the vasculature.2 Publications

Inductioni

Expressed with a circadian rhythm showing a broad peak in the late day and early night. Negatively regulated by LHY and CCA1.2 Publications

Gene expression databases

GenevestigatoriQ9LKL2.

Interactioni

Subunit structurei

Interacts with PIF1, PIL2, PIF3, PIF4, PIL5, PIL6, ABI3 (via C-terminus), ADO1/ZTL, ADO2, APRR3 and TCP21/CHE. Both the phosphorylated and the dephosphorylated forms interact with ADO1/ZLT.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ADO1Q94BT67EBI-618423,EBI-300691
APRR3Q9LVG43EBI-618423,EBI-1606968
APRR5Q6LA425EBI-618423,EBI-1536669
PIL1Q8L5W83EBI-618423,EBI-630752

Protein-protein interaction databases

BioGridi21503. 29 interactions.
DIPiDIP-33896N.
IntActiQ9LKL2. 19 interactions.
MINTiMINT-7908647.
STRINGi3702.AT5G61380.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9LKL2.
SMRiQ9LKL2. Positions 20-131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 138119Response regulatoryPROSITE-ProRule annotationAdd
BLAST
Domaini533 – 57543CCTPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili588 – 61023Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi595 – 60511Poly-GluAdd
BLAST

Domaini

The N-terminus (1-243) is required for interactions with ADO1/ZTL and APRR3.

Sequence similaritiesi

Belongs to the ARR-like family.Curated
Contains 1 CCT domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG242953.
HOGENOMiHOG000034017.
InParanoidiQ9LKL2.
KOiK12127.
OMAiHPASADY.
PhylomeDBiQ9LKL2.

Family and domain databases

InterProiIPR010402. CCT_domain.
IPR011006. CheY-like_superfamily.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF06203. CCT. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS51017. CCT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LKL2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLNGECKGG DGFIDRSRVR ILLCDNDSTS LGEVFTLLSE CSYQVTAVKS
60 70 80 90 100
ARQVIDALNA EGPDIDIILA EIDLPMAKGM KMLRYITRDK DLRRIPVIMM
110 120 130 140 150
SRQDEVPVVV KCLKLGAADY LVKPLRTNEL LNLWTHMWRR RRMLGLAEKN
160 170 180 190 200
MLSYDFDLVG SDQSDPNTNS TNLFSDDTDD RSLRSTNPQR GNLSHQENEW
210 220 230 240 250
SVATAPVHAR DGGLGADGTA TSSLAVTAIE PPLDHLAGSH HEPMKRNSNP
260 270 280 290 300
AQFSSAPKKS RLKIGESSAF FTYVKSTVLR TNGQDPPLVD GNGSLHLHRG
310 320 330 340 350
LAEKFQVVAS EGINNTKQAR RATPKSTVLR TNGQDPPLVN GNGSHHLHRG
360 370 380 390 400
AAEKFQVVAS EGINNTKQAH RSRGTEQYHS QGETLQNGAS YPHSLERSRT
410 420 430 440 450
LPTSMESHGR NYQEGNMNIP QVAMNRSKDS SQVDGSGFSA PNAYPYYMHG
460 470 480 490 500
VMNQVMMQSA AMMPQYGHQI PHCQPNHPNG MTGYPYYHHP MNTSLQHSQM
510 520 530 540 550
SLQNGQMSMV HHSWSPAGNP PSNEVRVNKL DRREEALLKF RRKRNQRCFD
560 570 580 590 600
KKIRYVNRKR LAERRPRVKG QFVRKMNGVN VDLNGQPDSA DYDDEEEEEE
610
EEEEENRDSS PQDDALGT
Length:618
Mass (Da):69,195
Last modified:September 30, 2000 - v1
Checksum:iEDBE0AA0B7FAB61E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti126 – 1261R → L in CAB75508 (PubMed:10926537).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041530 mRNA. Translation: BAA94547.1.
AF272039 mRNA. Translation: AAF86252.1.
AJ251086 mRNA. Translation: CAB75508.1.
AB010073 Genomic DNA. Translation: BAB08493.1.
CP002688 Genomic DNA. Translation: AED97460.1.
AY094393 mRNA. Translation: AAM19772.1.
BT005816 mRNA. Translation: AAO64751.1.
PIRiT52075.
T52076.
RefSeqiNP_200946.1. NM_125531.3.
UniGeneiAt.21919.

Genome annotation databases

EnsemblPlantsiAT5G61380.1; AT5G61380.1; AT5G61380.
GeneIDi836259.
KEGGiath:AT5G61380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041530 mRNA. Translation: BAA94547.1.
AF272039 mRNA. Translation: AAF86252.1.
AJ251086 mRNA. Translation: CAB75508.1.
AB010073 Genomic DNA. Translation: BAB08493.1.
CP002688 Genomic DNA. Translation: AED97460.1.
AY094393 mRNA. Translation: AAM19772.1.
BT005816 mRNA. Translation: AAO64751.1.
PIRiT52075.
T52076.
RefSeqiNP_200946.1. NM_125531.3.
UniGeneiAt.21919.

3D structure databases

ProteinModelPortaliQ9LKL2.
SMRiQ9LKL2. Positions 20-131.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21503. 29 interactions.
DIPiDIP-33896N.
IntActiQ9LKL2. 19 interactions.
MINTiMINT-7908647.
STRINGi3702.AT5G61380.1-P.

Proteomic databases

PRIDEiQ9LKL2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G61380.1; AT5G61380.1; AT5G61380.
GeneIDi836259.
KEGGiath:AT5G61380.

Organism-specific databases

GeneFarmi4313. 437.
TAIRiAT5G61380.

Phylogenomic databases

eggNOGiNOG242953.
HOGENOMiHOG000034017.
InParanoidiQ9LKL2.
KOiK12127.
OMAiHPASADY.
PhylomeDBiQ9LKL2.

Gene expression databases

GenevestigatoriQ9LKL2.

Family and domain databases

InterProiIPR010402. CCT_domain.
IPR011006. CheY-like_superfamily.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF06203. CCT. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS51017. CCT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genes encoding pseudo-response regulators: insight into His-to-Asp phosphorelay and circadian rhythm in Arabidopsis thaliana."
    Makino S., Kiba T., Imamura A., Hanaki N., Nakamura A., Suzuki T., Taniguchi M., Ueguchi C., Sugiyama T., Mizuno T.
    Plant Cell Physiol. 41:791-803(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, GENE FAMILY.
    Strain: cv. Columbia.
  2. "Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog."
    Strayer C., Oyama T., Schultz T.F., Raman R., Somers D.E., Mas P., Panda S., Kreps J.A., Kay S.A.
    Science 289:768-771(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF ALA-562.
    Strain: cv. Columbia.
  3. "Interactions of the developmental regulator ABI3 with proteins identified from developing Arabidopsis seeds."
    Kurup S., Jones H.D., Holdsworth M.J.
    Plant J. 21:143-155(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH ABI3.
    Strain: cv. Landsberg erecta.
  4. "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
    Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:41-54(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Circadian waves of expression of the APRR1/TOC1 family of pseudo-response regulators in Arabidopsis thaliana: insight into the plant circadian clock."
    Matsushika A., Makino S., Kojima M., Mizuno T.
    Plant Cell Physiol. 41:1002-1012(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Reciprocal regulation between TOC1 and LHY/CCA1 within the Arabidopsis circadian clock."
    Alabadi D., Oyama T., Yanovsky M.J., Harmon F.G., Mas P., Kay S.A.
    Science 293:880-883(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  9. "The APRR1/TOC1 quintet implicated in circadian rhythms of Arabidopsis thaliana: I. Characterization with APRR1-overexpressing plants."
    Makino S., Matsushika A., Kojima M., Yamashino T., Mizuno T.
    Plant Cell Physiol. 43:58-69(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PIF1.
  10. "A link between circadian-controlled bHLH factors and the APRR1/TOC1 quintet in Arabidopsis thaliana."
    Yamashino T., Matsushika A., Fujimori T., Sato S., Kato T., Tabata S., Mizuno T.
    Plant Cell Physiol. 44:619-629(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PIF1; PIL2; PIF3; PIF4; PIL5 AND PIL6.
  11. "Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis."
    Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T.
    J. Exp. Bot. 55:2015-2027(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ADO1 AND ADO2.
  12. Cited for: INTERACTION WITH ADO1.
  13. "PRR3 Is a vascular regulator of TOC1 stability in the Arabidopsis circadian clock."
    Para A., Farre E.M., Imaizumi T., Pruneda-Paz J.L., Harmon F.G., Kay S.A.
    Plant Cell 19:3462-3473(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INTERACTION WITH APRR3.
  14. "Post-translational regulation of the Arabidopsis circadian clock through selective proteolysis and phosphorylation of pseudo-response regulator proteins."
    Fujiwara S., Wang L., Han L., Suh S.-S., Salome P.A., McClung C.R., Somers D.E.
    J. Biol. Chem. 283:23073-23083(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION, INDUCTION, SUBCELLULAR LOCATION, INTERACTION WITH ADO1 AND APRR3.
  15. "CUL1 regulates TOC1 protein stability in the Arabidopsis circadian clock."
    Harmon F., Imaizumi T., Gray W.M.
    Plant J. 55:568-579(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  16. "A functional genomics approach reveals CHE as a component of the Arabidopsis circadian clock."
    Pruneda-Paz J.L., Breton G., Para A., Kay S.A.
    Science 323:1481-1485(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TCP21.

Entry informationi

Entry nameiAPRR1_ARATH
AccessioniPrimary (citable) accession number: Q9LKL2
Secondary accession number(s): Q9M4B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2004
Last sequence update: September 30, 2000
Last modified: January 6, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Subject of targeted degradation by the 26S proteasome. ZEITLUPE (ADO1/ZTL) is the F-box protein that associates with the SCF (for Skp/Cullin/F-box) E3 ubiquitin ligase that is responsible for marking APRR1/TOC1 for turnover. CUL1 is the functional cullin for the SCF(ZTL) complex. APRR3 binding competitively inhibits the ADO1/ZTL interaction.

Caution

Lacks the phospho-accepting Asp (here Glu-71), present in the receiver domain, which is one of the conserved features of the two-component response regulators (ARRs) family.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.