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Q9LKL2 (APRR1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Two-component response regulator-like APRR1
Alternative name(s):
ABI3-interacting protein 1
Pseudo-response regulator 1
Timing of CAB expression 1
Gene names
Name:APRR1
Synonyms:AIP1, TOC1
Ordered Locus Names:At5g61380
ORF Names:MFB13.16
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length618 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Controls photoperiodic flowering response. Component of the circadian clock. Expression of several members of the ARR-like family is controlled by circadian rhythm. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock. Positive regulator of CCA1 and LHY expression. Ref.7 Ref.16

Subunit structure

Interacts with PIF1, PIL2, PIF3, PIF4, PIL5, PIL6, ABI3 (via C-terminus), ADO1/ZTL, ADO2, APRR3 and TCP21/CHE. Both the phosphorylated and the dephosphorylated forms interact with ADO1/ZLT. Ref.3 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.16

Subcellular location

Nucleus Ref.14.

Tissue specificity

Expressed in leaves, flowers and siliques. Restricted to the vasculature. Ref.3 Ref.13

Induction

Expressed with a circadian rhythm showing a broad peak in the late day and early night. Negatively regulated by LHY and CCA1. Ref.8 Ref.14 Ref.15

Domain

The N-terminus (1-243) is required for interactions with ADO1/ZTL and APRR3.

Post-translational modification

Phosphorylated; during the day. Phosphorylation is required for optimal interaction with APRR3. Ref.14

Miscellaneous

Subject of targeted degradation by the 26S proteasome. ZEITLUPE (ADO1/ZTL) is the F-box protein that associates with the SCF (for Skp/Cullin/F-box) E3 ubiquitin ligase that is responsible for marking APRR1/TOC1 for turnover. CUL1 is the functional cullin for the SCF(ZTL) complex. APRR3 binding competitively inhibits the ADO1/ZTL interaction.

Sequence similarities

Belongs to the ARR-like family.

Contains 1 CCT domain.

Contains 1 response regulatory domain.

Caution

Lacks the phospho-accepting Asp (here Glu-71), present in the receiver domain, which is one of the conserved features of the two-component response regulators (ARRs) family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 618618Two-component response regulator-like APRR1
PRO_0000081435

Regions

Domain20 – 138119Response regulatory
Domain533 – 57543CCT
Coiled coil588 – 61023 Potential
Compositional bias595 – 60511Poly-Glu

Experimental info

Mutagenesis5621A → V in toc1-1; causes shortened circadian rhythms in light-grown plants. Ref.2
Sequence conflict1261R → L in CAB75508. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9LKL2 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: EDBE0AA0B7FAB61E

FASTA61869,195
        10         20         30         40         50         60 
MDLNGECKGG DGFIDRSRVR ILLCDNDSTS LGEVFTLLSE CSYQVTAVKS ARQVIDALNA 

        70         80         90        100        110        120 
EGPDIDIILA EIDLPMAKGM KMLRYITRDK DLRRIPVIMM SRQDEVPVVV KCLKLGAADY 

       130        140        150        160        170        180 
LVKPLRTNEL LNLWTHMWRR RRMLGLAEKN MLSYDFDLVG SDQSDPNTNS TNLFSDDTDD 

       190        200        210        220        230        240 
RSLRSTNPQR GNLSHQENEW SVATAPVHAR DGGLGADGTA TSSLAVTAIE PPLDHLAGSH 

       250        260        270        280        290        300 
HEPMKRNSNP AQFSSAPKKS RLKIGESSAF FTYVKSTVLR TNGQDPPLVD GNGSLHLHRG 

       310        320        330        340        350        360 
LAEKFQVVAS EGINNTKQAR RATPKSTVLR TNGQDPPLVN GNGSHHLHRG AAEKFQVVAS 

       370        380        390        400        410        420 
EGINNTKQAH RSRGTEQYHS QGETLQNGAS YPHSLERSRT LPTSMESHGR NYQEGNMNIP 

       430        440        450        460        470        480 
QVAMNRSKDS SQVDGSGFSA PNAYPYYMHG VMNQVMMQSA AMMPQYGHQI PHCQPNHPNG 

       490        500        510        520        530        540 
MTGYPYYHHP MNTSLQHSQM SLQNGQMSMV HHSWSPAGNP PSNEVRVNKL DRREEALLKF 

       550        560        570        580        590        600 
RRKRNQRCFD KKIRYVNRKR LAERRPRVKG QFVRKMNGVN VDLNGQPDSA DYDDEEEEEE 

       610 
EEEEENRDSS PQDDALGT 

« Hide

References

« Hide 'large scale' references
[1]"Genes encoding pseudo-response regulators: insight into His-to-Asp phosphorelay and circadian rhythm in Arabidopsis thaliana."
Makino S., Kiba T., Imamura A., Hanaki N., Nakamura A., Suzuki T., Taniguchi M., Ueguchi C., Sugiyama T., Mizuno T.
Plant Cell Physiol. 41:791-803(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, GENE FAMILY.
Strain: cv. Columbia.
[2]"Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog."
Strayer C., Oyama T., Schultz T.F., Raman R., Somers D.E., Mas P., Panda S., Kreps J.A., Kay S.A.
Science 289:768-771(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF ALA-562.
Strain: cv. Columbia.
[3]"Interactions of the developmental regulator ABI3 with proteins identified from developing Arabidopsis seeds."
Kurup S., Jones H.D., Holdsworth M.J.
Plant J. 21:143-155(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH ABI3.
Strain: cv. Landsberg erecta.
[4]"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[6]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"Circadian waves of expression of the APRR1/TOC1 family of pseudo-response regulators in Arabidopsis thaliana: insight into the plant circadian clock."
Matsushika A., Makino S., Kojima M., Mizuno T.
Plant Cell Physiol. 41:1002-1012(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Reciprocal regulation between TOC1 and LHY/CCA1 within the Arabidopsis circadian clock."
Alabadi D., Oyama T., Yanovsky M.J., Harmon F.G., Mas P., Kay S.A.
Science 293:880-883(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[9]"The APRR1/TOC1 quintet implicated in circadian rhythms of Arabidopsis thaliana: I. Characterization with APRR1-overexpressing plants."
Makino S., Matsushika A., Kojima M., Yamashino T., Mizuno T.
Plant Cell Physiol. 43:58-69(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PIF1.
[10]"A link between circadian-controlled bHLH factors and the APRR1/TOC1 quintet in Arabidopsis thaliana."
Yamashino T., Matsushika A., Fujimori T., Sato S., Kato T., Tabata S., Mizuno T.
Plant Cell Physiol. 44:619-629(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PIF1; PIL2; PIF3; PIF4; PIL5 AND PIL6.
[11]"Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis."
Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T.
J. Exp. Bot. 55:2015-2027(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ADO1 AND ADO2.
[12]"Forward genetic analysis of the circadian clock separates the multiple functions of ZEITLUPE."
Kevei E., Gyula P., Hall A., Kozma-Bognar L., Kim W.Y., Eriksson M.E., Toth R., Hanano S., Feher B., Southern M.M., Bastow R.M., Viczian A., Hibberd V., Davis S.J., Somers D.E., Nagy F., Millar A.J.
Plant Physiol. 140:933-945(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ADO1.
[13]"PRR3 Is a vascular regulator of TOC1 stability in the Arabidopsis circadian clock."
Para A., Farre E.M., Imaizumi T., Pruneda-Paz J.L., Harmon F.G., Kay S.A.
Plant Cell 19:3462-3473(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INTERACTION WITH APRR3.
[14]"Post-translational regulation of the Arabidopsis circadian clock through selective proteolysis and phosphorylation of pseudo-response regulator proteins."
Fujiwara S., Wang L., Han L., Suh S.-S., Salome P.A., McClung C.R., Somers D.E.
J. Biol. Chem. 283:23073-23083(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION, INDUCTION, SUBCELLULAR LOCATION, INTERACTION WITH ADO1 AND APRR3.
[15]"CUL1 regulates TOC1 protein stability in the Arabidopsis circadian clock."
Harmon F., Imaizumi T., Gray W.M.
Plant J. 55:568-579(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
[16]"A functional genomics approach reveals CHE as a component of the Arabidopsis circadian clock."
Pruneda-Paz J.L., Breton G., Para A., Kay S.A.
Science 323:1481-1485(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TCP21.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB041530 mRNA. Translation: BAA94547.1.
AF272039 mRNA. Translation: AAF86252.1.
AJ251086 mRNA. Translation: CAB75508.1.
AB010073 Genomic DNA. Translation: BAB08493.1.
CP002688 Genomic DNA. Translation: AED97460.1.
AY094393 mRNA. Translation: AAM19772.1.
BT005816 mRNA. Translation: AAO64751.1.
PIRT52075.
T52076.
RefSeqNP_200946.1. NM_125531.3.
UniGeneAt.21919.

3D structure databases

ProteinModelPortalQ9LKL2.
SMRQ9LKL2. Positions 20-131.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid21503. 29 interactions.
DIPDIP-33896N.
IntActQ9LKL2. 19 interactions.
MINTMINT-7908647.
STRING3702.AT5G61380.1-P.

Proteomic databases

PRIDEQ9LKL2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G61380.1; AT5G61380.1; AT5G61380.
GeneID836259.
KEGGath:AT5G61380.

Organism-specific databases

GeneFarm4313. 437.
TAIRAT5G61380.

Phylogenomic databases

eggNOGNOG242953.
HOGENOMHOG000034017.
InParanoidQ9LKL2.
KOK12127.
OMAFVRQASN.
PhylomeDBQ9LKL2.

Gene expression databases

GenevestigatorQ9LKL2.

Family and domain databases

InterProIPR010402. CCT_domain.
IPR011006. CheY-like_superfamily.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamPF06203. CCT. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
SMARTSM00448. REC. 1 hit.
[Graphical view]
SUPFAMSSF52172. SSF52172. 1 hit.
PROSITEPS51017. CCT. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAPRR1_ARATH
AccessionPrimary (citable) accession number: Q9LKL2
Secondary accession number(s): Q9M4B7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names