Reviewed, UniProtKB/Swiss-Prot Q9LKL2 (APRR1_ARATH)

Names and origin

Protein names

Recommended name:
    Two-component response regulator-like APRR1
Alternative name(s):
    Pseudo-response regulator 1
    Timing of CAB expression 1
    ABI3-interacting protein 1

Gene names

Name:	APRR1
Synonyms:	AIP1, TOC1
Ordered Locus Names:	At5g61380
ORF Names:	MFB13.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Controls photoperiodic flowering response. Seems to be one of the component of the circadian clock. Expression of several members of the ARR-like family is controlled by circadian rhythm. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock.
Subunit structure	Interacts with ABI3. Also interacts with ADO1 and ADO2.
Subcellular location	Nucleus.
Tissue specificity	Expressed in leaves, flowers and siliques.
Induction	Expressed with a circadian rhythm showing a broad peak in the late day. Negatively regulated by LHY and CCA1.
Sequence similarities	Belongs to the ARR-like family. Contains 1 CCT domain. Contains 1 response regulatory domain.
Caution	Lacks the phospho-accepting Asp (here Glu-71), present in the receiver domain, which is one of the conserved features of the two-component response regulators (ARRs) family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Nucleus
Domain	Coiled coil
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	protein binding Inferred from physical interaction. Source: IntAct transcription regulator activity Traceable author statement. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ADO1	Q94BT6	4	EBI-618423,EBI-300691
ADO2	Q8W420	2	EBI-618423,EBI-1015688
APRR3	Q9LVG4	1	EBI-618423,EBI-1606968
At2g46970	O80727	3	EBI-618423,EBI-630752

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 618	618	Two-component response regulator-like APRR1
Regions
	Domain	20 – 138	119	Response regulatory
	Domain	533 – 575	43	CCT
	Coiled coil	588 – 610	23	Potential
	Compositional bias	595 – 605	11	Poly-Glu
Experimental info
	Mutagenesis	562	1	A → V in toc1-1; causes shortened circadian rhythms in light-grown plants
	Sequence conflict	126	1	R → L in CAB75508. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9LKL2-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: EDBE0AA0B7FAB61E	FASTA	618	69,195
	10 20 30 40 50 60 MDLNGECKGG DGFIDRSRVR ILLCDNDSTS LGEVFTLLSE CSYQVTAVKS ARQVIDALNA 70 80 90 100 110 120 EGPDIDIILA EIDLPMAKGM KMLRYITRDK DLRRIPVIMM SRQDEVPVVV KCLKLGAADY 130 140 150 160 170 180 LVKPLRTNEL LNLWTHMWRR RRMLGLAEKN MLSYDFDLVG SDQSDPNTNS TNLFSDDTDD 190 200 210 220 230 240 RSLRSTNPQR GNLSHQENEW SVATAPVHAR DGGLGADGTA TSSLAVTAIE PPLDHLAGSH 250 260 270 280 290 300 HEPMKRNSNP AQFSSAPKKS RLKIGESSAF FTYVKSTVLR TNGQDPPLVD GNGSLHLHRG 310 320 330 340 350 360 LAEKFQVVAS EGINNTKQAR RATPKSTVLR TNGQDPPLVN GNGSHHLHRG AAEKFQVVAS 370 380 390 400 410 420 EGINNTKQAH RSRGTEQYHS QGETLQNGAS YPHSLERSRT LPTSMESHGR NYQEGNMNIP 430 440 450 460 470 480 QVAMNRSKDS SQVDGSGFSA PNAYPYYMHG VMNQVMMQSA AMMPQYGHQI PHCQPNHPNG 490 500 510 520 530 540 MTGYPYYHHP MNTSLQHSQM SLQNGQMSMV HHSWSPAGNP PSNEVRVNKL DRREEALLKF 550 560 570 580 590 600 RRKRNQRCFD KKIRYVNRKR LAERRPRVKG QFVRKMNGVN VDLNGQPDSA DYDDEEEEEE 610 EEEEENRDSS PQDDALGT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genes encoding pseudo-response regulators: insight into His-to-Asp phosphorelay and circadian rhythm in Arabidopsis thaliana." Makino S., Kiba T., Imamura A., Hanaki N., Nakamura A., Suzuki T., Taniguchi M., Ueguchi C., Sugiyama T., Mizuno T. Plant Cell Physiol. 41:791-803(2000) [PubMed: 10945350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, CHARACTERIZATION, GENE FAMILY. Strain: cv. Columbia.
[2]	"Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog." Strayer C., Oyama T., Schultz T.F., Raman R., Somers D.E., Mas P., Panda S., Kreps J.A., Kay S.A. Science 289:768-771(2000) [PubMed: 10926537] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF ALA-562. Strain: cv. Columbia.
[3]	"Interactions of the developmental regulator ABI3 with proteins identified from developing Arabidopsis seeds." Kurup S., Jones H.D., Holdsworth M.J. Plant J. 21:143-155(2000) [PubMed: 10743655] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH ABI3. Strain: cv. Landsberg erecta.
[4]	"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones." Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:41-54(1998) [PubMed: 9628582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Circadian waves of expression of the APRR1/TOC1 family of pseudo-response regulators in Arabidopsis thaliana: insight into the plant circadian clock." Matsushika A., Makino S., Kojima M., Mizuno T. Plant Cell Physiol. 41:1002-1012(2000) [PubMed: 11100772] [Abstract] Cited for: FUNCTION.
[7]	"Reciprocal regulation between TOC1 and LHY/CCA1 within the Arabidopsis circadian clock." Alabadi D., Oyama T., Yanovsky M.J., Harmon F.G., Mas P., Kay S.A. Science 293:880-883(2001) [PubMed: 11486091] [Abstract] Cited for: INDUCTION.
[8]	"Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis." Yasuhara M., Mitsui S., Hirano H., Takanabe R., Tokioka Y., Ihara N., Komatsu A., Seki M., Shinozaki K., Kiyosue T. J. Exp. Bot. 55:2015-2027(2004) [PubMed: 15310821] [Abstract] Cited for: INTERACTION WITH ADO1 AND ADO2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB041530 mRNA. Translation: BAA94547.1. AF272039 mRNA. Translation: AAF86252.1. AJ251086 mRNA. Translation: CAB75508.1. AB010073 Genomic DNA. Translation: BAB08493.1. AY094393 mRNA. Translation: AAM19772.1. BT005816 mRNA. Translation: AAO64751.1.
PIR	T52075. T52076.
RefSeq	NP_200946.1.
UniGene	At.21919
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LKL2.
Genome annotation databases
GeneID	836259.
GenomeReviews	Gene locus AT5G61380 in contig BA000015_GR.
KEGG	ath:AT5G61380.
NMPDR	fig|3702.1.peg.28159.
Organism-specific databases
GeneFarm	4313. 437.
TAIR	At5g61380.
Gene expression databases
ArrayExpress	Q9LKL2.
GermOnline	AT5G61380. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR010402. CCT. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Pfam	PF06203. CCT. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
ProDom	PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS51017. CCT. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

APRR1_ARATH

Accession

Primary (citable) accession number: Q9LKL2
Secondary accession number(s): Q9M4B7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	October 1, 2000
Last modified:	July 22, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)