Q9LKJ3 (PHSH_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Alpha-glucan phosphorylase, H isozyme EC=2.4.1.1 Alternative name(s): Starch phosphorylase H
Organism	Triticum aestivum (Wheat)
Taxonomic identifier	4565 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	832 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties By similarity.
Catalytic activity	(1,4-alpha-D-glucosyl)(n) + phosphate = (1,4-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.
Cofactor	Pyridoxal phosphate.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycogen phosphorylase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Glycosyltransferase Transferase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphorylase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 832	832	Alpha-glucan phosphorylase, H isozyme		PRO_0000188543
Amino acid modifications
	Modified residue	678	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LKJ3 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: BEF45CA8EDCBB466	FASTA	832	93,612
	10 20 30 40 50 60 MSAADKVKPA ASPASEDPSA IAGNISYHAQ YSPHFSPLAF GPEQAFYATA ESVRDHLLQR 70 80 90 100 110 120 WNDTYLHFHK TDPKQTYYLS MEYLQGRALT NAVGNLAITG AYADALKKFG YELEAIAGQE 130 140 150 160 170 180 RDAALGNGGL GRLASCFLDS MATLNLPSWG YGLRYRYGLF KQRIAKEGQE EIAEDWLDKF 190 200 210 220 230 240 SPWEIVRHDV VYPIRFFGHV EISPDGKRKW AGGEVLNALA YDVPIPGYKT KNAISLRLWD 250 260 270 280 290 300 ATATAEDFNL FQFNDGQYES AAQLHSRAQQ ICAVLYPGDA TEEGKLLRLK QQYFLCSASL 310 320 330 340 350 360 QDIIFRFKER KADRVSGKWS EFPSKVAVQM NDTHPTLAIP ELMRLLMDVE GLGWDEAWAV 370 380 390 400 410 420 TNKTVAYTNH TVLPEALEKW SQAVMKKLLP RHMEIIEEID KRFREMVIST RKDMEGKIES 430 440 450 460 470 480 MRVLDNNPEK PVVRMANLCV VAGHTVNGVA ELHSNILKQE LFADYVSIWP NKFQNKTNGI 490 500 510 520 530 540 TPRRWLRFCN PELSEIVTKW LKTDQWTSNL DLLTGLRKFA DDEKLHAEWA AAKLASKKRL 550 560 570 580 590 600 AKHVLDVTGV TIDPDSLFDI QIKRIHEYKR QLMNILGAVY RYKKLKEMSA ADRQKVTPRT 610 620 630 640 650 660 VMVGGKAFAT YTNAKRIVKL VNDVGAVVNN DADVNKYLKV VFIPNYNVSV AEVLIPGSEL 670 680 690 700 710 720 SQHISTAGME ASGTSNMKFS LNGCVIIGTL DGANVEIREE VGQDNFFLFG AKADQVAGLR 730 740 750 760 770 780 KDRENGLFKP DPRFEEAKQF IRSGAFGTYD YTPLLDSLEG NTGFGRGDYF LVGYDFPSYI 790 800 810 820 830 DAQARVDEAY KDKKKWVKMS ILNTAGSGKF SSDRTIDQYA KEIWGISACP VP

References

[1]	"Full length clone of a cytosolic wheat leaf starch phosphorylase." Schupp N.T., Ziegler P., Huebsch S.D. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Star. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF275551 mRNA. Translation: AAF82787.1.
UniGene	Ta.1197.
3D structure databases
ProteinModelPortal	Q9LKJ3.
ModBase	Search...
Protein family/group databases
CAZy	GT35. Glycosyltransferase Family 35.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	Q9LKJ3.
Enzyme and pathway databases
BRENDA	2.4.1.1. 6500.
Family and domain databases
InterPro	IPR011833. Glycg_phsphrylas. IPR000811. Glyco_trans_35. [Graphical view]
PANTHER	PTHR11468. Glyco_trans_35. 1 hit.
Pfam	PF00343. Phosphorylase. 1 hit. [Graphical view]
PIRSF	PIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMs	TIGR02093. P_ylase. 1 hit.
PROSITE	PS00102. PHOSPHORYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHSH_WHEAT

Accession

Primary (citable) accession number: Q9LKJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	October 1, 2000
Last modified:	May 31, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program