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Q9LKA3 (MDHM2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase 2, mitochondrial

EC=1.1.1.37
Alternative name(s):
mNAD-MDH 2
Gene names
Ordered Locus Names:At3g15020
ORF Names:K15M2.16
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion matrix Ref.4.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LKA3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2222Mitochondrion By similarity
Chain23 – 341319Malate dehydrogenase 2, mitochondrial
PRO_0000224148

Regions

Nucleotide binding36 – 427NAD By similarity
Nucleotide binding145 – 1473NAD By similarity

Sites

Active site2051Proton acceptor By similarity
Binding site621NAD By similarity
Binding site1091Substrate By similarity
Binding site1151Substrate By similarity
Binding site1221NAD By similarity
Binding site1471Substrate By similarity
Binding site1811Substrate By similarity
Binding site2561NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 38364CAE36712AE1

FASTA34135,875
        10         20         30         40         50         60 
MFRSMIVRSA SPVKQGLLRR GFASESVPDR KVVILGAAGG IGQPLSLLMK LNPLVSSLSL 

        70         80         90        100        110        120 
YDIANTPGVA ADVGHINTRS QVSGYMGDDD LGKALEGADL VIIPAGVPRK PGMTRDDLFN 

       130        140        150        160        170        180 
INAGIVKNLS IAIAKYCPQA LVNMISNPVN STVPIAAEIF KKAGTYDEKK LFGVTTLDVV 

       190        200        210        220        230        240 
RARTFYAGKS DVNVAEVNVP VVGGHAGITI LPLFSQASPQ ANLSDDLIRA LTKRTQDGGT 

       250        260        270        280        290        300 
EVVEAKAGKG SATLSMAYAG ALFADACLKG LNGVPNVVEC SFVQSTITEL PFFASKVRLG 

       310        320        330        340 
KNGVEEVLDL GPLSDFEKEG LEALKAELKS SIEKGIKFAN Q 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Landsberg erecta.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP000370 Genomic DNA. Translation: BAA97065.1.
CP002686 Genomic DNA. Translation: AEE75604.1.
AY045592 mRNA. Translation: AAK73950.1.
AY093788 mRNA. Translation: AAM10404.1.
RefSeqNP_188120.1. NM_112364.3.
UniGeneAt.6661.
At.75599.

3D structure databases

ProteinModelPortalQ9LKA3.
SMRQ9LKA3. Positions 31-338.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid6065. 1 interaction.

Proteomic databases

PaxDbQ9LKA3.
PRIDEQ9LKA3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G15020.1; AT3G15020.1; AT3G15020. [Q9LKA3-1]
GeneID820731.
KEGGath:AT3G15020.

Organism-specific databases

GeneFarm2027. 159.
TAIRAT3G15020.

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000213792.
InParanoidQ9LKA3.
KOK00026.
OMAGIKFATQ.
PhylomeDBQ9LKA3.
ProtClustDBPLN00106.

Enzyme and pathway databases

BioCycARA:GQT-2283-MONOMER.

Gene expression databases

GenevestigatorQ9LKA3.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PTHR11540:SF1. PTHR11540:SF1. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDHM2_ARATH
AccessionPrimary (citable) accession number: Q9LKA3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names