Reviewed, UniProtKB/Swiss-Prot Q9LKA3 (MDHM2_ARATH)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase 2, mitochondrial
    EC=1.1.1.37
Alternative name(s):
    mNAD-MDH 2

Gene names

Ordered Locus Names:	At3g15020
ORF Names:	K15M2.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion matrix. Ref.3
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	defense response to bacterium Inferred from expression pattern. Source: TAIR glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from direct assay. Source: TAIR membrane Inferred from direct assay. Source: TAIR mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LKA3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 22	22	Mitochondrion By similarity
	Chain	23 – 341	319	Malate dehydrogenase 2, mitochondrial		PRO_0000224148
Regions
	Nucleotide binding	36 – 42	7	NAD By similarity
	Nucleotide binding	145 – 147	3	NAD By similarity
Sites
	Active site	205	1	Proton acceptor By similarity
	Binding site	62	1	NAD By similarity
	Binding site	109	1	Substrate By similarity
	Binding site	115	1	Substrate By similarity
	Binding site	122	1	NAD By similarity
	Binding site	147	1	Substrate By similarity
	Binding site	181	1	Substrate By similarity
	Binding site	256	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 38364CAE36712AE1	FASTA	341	35,875
	10 20 30 40 50 60 MFRSMIVRSA SPVKQGLLRR GFASESVPDR KVVILGAAGG IGQPLSLLMK LNPLVSSLSL 70 80 90 100 110 120 YDIANTPGVA ADVGHINTRS QVSGYMGDDD LGKALEGADL VIIPAGVPRK PGMTRDDLFN 130 140 150 160 170 180 INAGIVKNLS IAIAKYCPQA LVNMISNPVN STVPIAAEIF KKAGTYDEKK LFGVTTLDVV 190 200 210 220 230 240 RARTFYAGKS DVNVAEVNVP VVGGHAGITI LPLFSQASPQ ANLSDDLIRA LTKRTQDGGT 250 260 270 280 290 300 EVVEAKAGKG SATLSMAYAG ALFADACLKG LNGVPNVVEC SFVQSTITEL PFFASKVRLG 310 320 330 340 KNGVEEVLDL GPLSDFEKEG LEALKAELKS SIEKGIKFAN Q

References

[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP000370 Genomic DNA. Translation: BAA97065.1. AY045592 mRNA. Translation: AAK73950.1. AY093788 mRNA. Translation: AAM10404.1.
IPI	IPI00541866.
RefSeq	NP_188120.1.
UniGene	At.6661 At.71684
3D structure databases
HSSP	HSSP built from PDB template 1MLD based on UniProtKB P00346.
ModBase	Search...
Proteomic databases
PRIDE	Q9LKA3.
Genome annotation databases
GeneID	820731.
GenomeReviews	Gene locus AT3G15020 in contig BA000014_GR.
NMPDR	fig|3702.1.peg.13650.
Organism-specific databases
GeneFarm	2027. 159.
TAIR	At3g15020.
Phylogenomic databases
OMA	Q9LKA3. FGCAVEL.
Enzyme and pathway databases
BRENDA	1.1.1.37. 302.
Gene expression databases
GermOnline	AT3G15020. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_NAD-dep_euk_g_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11540:SF1. MDH_euk_g_bac. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDHM2_ARATH

Accession

Primary (citable) accession number: Q9LKA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)