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Q9LKA3

- MDHM2_ARATH

UniProt

Q9LKA3 - MDHM2_ARATH

Protein

Malate dehydrogenase 2, mitochondrial

Gene

At3g15020

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei62 – 621NADBy similarity
    Binding sitei109 – 1091SubstratePROSITE-ProRule annotation
    Binding sitei115 – 1151SubstratePROSITE-ProRule annotation
    Binding sitei122 – 1221NADBy similarity
    Binding sitei147 – 1471SubstratePROSITE-ProRule annotation
    Binding sitei181 – 1811SubstratePROSITE-ProRule annotation
    Active sitei205 – 2051Proton acceptorBy similarity
    Binding sitei256 – 2561NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi36 – 427NADBy similarity
    Nucleotide bindingi145 – 1473NADBy similarity

    GO - Molecular functioni

    1. copper ion binding Source: TAIR
    2. L-malate dehydrogenase activity Source: TAIR

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. defense response to bacterium Source: TAIR
    3. malate metabolic process Source: InterPro
    4. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciARA:GQT-2283-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase 2, mitochondrial (EC:1.1.1.37)
    Alternative name(s):
    mNAD-MDH 2
    Gene namesi
    Ordered Locus Names:At3g15020
    ORF Names:K15M2.16
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G15020.

    Subcellular locationi

    Mitochondrion matrix 1 Publication

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. membrane Source: TAIR
    3. mitochondrial matrix Source: UniProtKB-SubCell
    4. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2222MitochondrionBy similarityAdd
    BLAST
    Chaini23 – 341319Malate dehydrogenase 2, mitochondrialPRO_0000224148Add
    BLAST

    Proteomic databases

    PaxDbiQ9LKA3.
    PRIDEiQ9LKA3.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9LKA3.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi6065. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LKA3.
    SMRiQ9LKA3. Positions 31-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0039.
    HOGENOMiHOG000213792.
    InParanoidiQ9LKA3.
    KOiK00026.
    OMAiLCTAISK.
    PhylomeDBiQ9LKA3.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9LKA3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MFRSMIVRSA SPVKQGLLRR GFASESVPDR KVVILGAAGG IGQPLSLLMK    50
    LNPLVSSLSL YDIANTPGVA ADVGHINTRS QVSGYMGDDD LGKALEGADL 100
    VIIPAGVPRK PGMTRDDLFN INAGIVKNLS IAIAKYCPQA LVNMISNPVN 150
    STVPIAAEIF KKAGTYDEKK LFGVTTLDVV RARTFYAGKS DVNVAEVNVP 200
    VVGGHAGITI LPLFSQASPQ ANLSDDLIRA LTKRTQDGGT EVVEAKAGKG 250
    SATLSMAYAG ALFADACLKG LNGVPNVVEC SFVQSTITEL PFFASKVRLG 300
    KNGVEEVLDL GPLSDFEKEG LEALKAELKS SIEKGIKFAN Q 341
    Length:341
    Mass (Da):35,875
    Last modified:October 1, 2000 - v1
    Checksum:i38364CAE36712AE1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP000370 Genomic DNA. Translation: BAA97065.1.
    CP002686 Genomic DNA. Translation: AEE75604.1.
    AY045592 mRNA. Translation: AAK73950.1.
    AY093788 mRNA. Translation: AAM10404.1.
    RefSeqiNP_188120.1. NM_112364.3. [Q9LKA3-1]
    UniGeneiAt.6661.
    At.75599.

    Genome annotation databases

    EnsemblPlantsiAT3G15020.1; AT3G15020.1; AT3G15020. [Q9LKA3-1]
    GeneIDi820731.
    KEGGiath:AT3G15020.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP000370 Genomic DNA. Translation: BAA97065.1 .
    CP002686 Genomic DNA. Translation: AEE75604.1 .
    AY045592 mRNA. Translation: AAK73950.1 .
    AY093788 mRNA. Translation: AAM10404.1 .
    RefSeqi NP_188120.1. NM_112364.3. [Q9LKA3-1 ]
    UniGenei At.6661.
    At.75599.

    3D structure databases

    ProteinModelPortali Q9LKA3.
    SMRi Q9LKA3. Positions 31-338.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 6065. 1 interaction.

    Proteomic databases

    PaxDbi Q9LKA3.
    PRIDEi Q9LKA3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G15020.1 ; AT3G15020.1 ; AT3G15020 . [Q9LKA3-1 ]
    GeneIDi 820731.
    KEGGi ath:AT3G15020.

    Organism-specific databases

    GeneFarmi 2027. 159.
    TAIRi AT3G15020.

    Phylogenomic databases

    eggNOGi COG0039.
    HOGENOMi HOG000213792.
    InParanoidi Q9LKA3.
    KOi K00026.
    OMAi LCTAISK.
    PhylomeDBi Q9LKA3.

    Enzyme and pathway databases

    BioCyci ARA:GQT-2283-MONOMER.

    Gene expression databases

    Genevestigatori Q9LKA3.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
      DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.

    Entry informationi

    Entry nameiMDHM2_ARATH
    AccessioniPrimary (citable) accession number: Q9LKA3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2006
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3