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Protein

Malate dehydrogenase 2, mitochondrial

Gene

At3g15020

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621NADBy similarity
Binding sitei109 – 1091SubstratePROSITE-ProRule annotation
Binding sitei115 – 1151SubstratePROSITE-ProRule annotation
Binding sitei122 – 1221NADBy similarity
Binding sitei147 – 1471SubstratePROSITE-ProRule annotation
Binding sitei181 – 1811SubstratePROSITE-ProRule annotation
Active sitei205 – 2051Proton acceptorBy similarity
Binding sitei256 – 2561NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 427NADBy similarity
Nucleotide bindingi145 – 1473NADBy similarity

GO - Molecular functioni

  1. copper ion binding Source: TAIR
  2. L-malate dehydrogenase activity Source: TAIR

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. defense response to bacterium Source: TAIR
  3. malate metabolic process Source: InterPro
  4. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciARA:GQT-2283-MONOMER.
BRENDAi1.1.1.37. 399.
ReactomeiREACT_289406. Citric acid cycle (TCA cycle).
REACT_298151. Gluconeogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase 2, mitochondrial (EC:1.1.1.37)
Alternative name(s):
mNAD-MDH 2
Gene namesi
Ordered Locus Names:At3g15020
ORF Names:K15M2.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G15020.

Subcellular locationi

  1. Mitochondrion matrix 1 Publication

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. membrane Source: TAIR
  3. mitochondrial matrix Source: UniProtKB-SubCell
  4. mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2222MitochondrionBy similarityAdd
BLAST
Chaini23 – 341319Malate dehydrogenase 2, mitochondrialPRO_0000224148Add
BLAST

Proteomic databases

PaxDbiQ9LKA3.
PRIDEiQ9LKA3.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LKA3. baseline and differential.
GenevestigatoriQ9LKA3.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi6065. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9LKA3.
SMRiQ9LKA3. Positions 31-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213792.
InParanoidiQ9LKA3.
KOiK00026.
OMAiCPKALVA.
PhylomeDBiQ9LKA3.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LKA3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFRSMIVRSA SPVKQGLLRR GFASESVPDR KVVILGAAGG IGQPLSLLMK
60 70 80 90 100
LNPLVSSLSL YDIANTPGVA ADVGHINTRS QVSGYMGDDD LGKALEGADL
110 120 130 140 150
VIIPAGVPRK PGMTRDDLFN INAGIVKNLS IAIAKYCPQA LVNMISNPVN
160 170 180 190 200
STVPIAAEIF KKAGTYDEKK LFGVTTLDVV RARTFYAGKS DVNVAEVNVP
210 220 230 240 250
VVGGHAGITI LPLFSQASPQ ANLSDDLIRA LTKRTQDGGT EVVEAKAGKG
260 270 280 290 300
SATLSMAYAG ALFADACLKG LNGVPNVVEC SFVQSTITEL PFFASKVRLG
310 320 330 340
KNGVEEVLDL GPLSDFEKEG LEALKAELKS SIEKGIKFAN Q
Length:341
Mass (Da):35,875
Last modified:October 1, 2000 - v1
Checksum:i38364CAE36712AE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000370 Genomic DNA. Translation: BAA97065.1.
CP002686 Genomic DNA. Translation: AEE75604.1.
AY045592 mRNA. Translation: AAK73950.1.
AY093788 mRNA. Translation: AAM10404.1.
RefSeqiNP_188120.1. NM_112364.3. [Q9LKA3-1]
UniGeneiAt.6661.
At.75599.

Genome annotation databases

EnsemblPlantsiAT3G15020.1; AT3G15020.1; AT3G15020. [Q9LKA3-1]
GeneIDi820731.
KEGGiath:AT3G15020.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000370 Genomic DNA. Translation: BAA97065.1.
CP002686 Genomic DNA. Translation: AEE75604.1.
AY045592 mRNA. Translation: AAK73950.1.
AY093788 mRNA. Translation: AAM10404.1.
RefSeqiNP_188120.1. NM_112364.3. [Q9LKA3-1]
UniGeneiAt.6661.
At.75599.

3D structure databases

ProteinModelPortaliQ9LKA3.
SMRiQ9LKA3. Positions 31-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6065. 1 interaction.

Proteomic databases

PaxDbiQ9LKA3.
PRIDEiQ9LKA3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G15020.1; AT3G15020.1; AT3G15020. [Q9LKA3-1]
GeneIDi820731.
KEGGiath:AT3G15020.

Organism-specific databases

GeneFarmi2027. 159.
TAIRiAT3G15020.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213792.
InParanoidiQ9LKA3.
KOiK00026.
OMAiCPKALVA.
PhylomeDBiQ9LKA3.

Enzyme and pathway databases

BioCyciARA:GQT-2283-MONOMER.
BRENDAi1.1.1.37. 399.
ReactomeiREACT_289406. Citric acid cycle (TCA cycle).
REACT_298151. Gluconeogenesis.

Miscellaneous databases

PROiQ9LKA3.

Gene expression databases

ExpressionAtlasiQ9LKA3. baseline and differential.
GenevestigatoriQ9LKA3.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Landsberg erecta.

Entry informationi

Entry nameiMDHM2_ARATH
AccessioniPrimary (citable) accession number: Q9LKA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: October 1, 2000
Last modified: April 29, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.