Q9LK94 (MDAR2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable monodehydroascorbate reductase, cytoplasmic isoform 2
Short name=MDAR 2
EC=1.6.5.4

Gene names

Ordered Locus Names:	At3g27820
ORF Names:	K16N12.2

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the conversion of monodehydroascorbate to ascorbate, oxidizing NADH in the process By similarity.
Catalytic activity	NADH + 2 monodehydroascorbate = NAD+ + 2 ascorbate.
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from mutant phenotype. Source: TAIR
Cellular component	chloroplast envelope Inferred from direct assay. Source: TAIR peroxisomal membrane Inferred from direct assay. Source: TAIR
Molecular function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro monodehydroascorbate reductase (NADH) activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Probable monodehydroascorbate reductase, cytoplasmic isoform 2		PRO_0000209137

Sequences

	Sequence		Length	Mass (Da)
	Q9LK94 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: AC21B11991178DFA	FASTA	488	53,526
	10 20 30 40 50 60 MGRAFVYVIL GGGVAAGYAA LEFTRRGVSD GELCIISEEP VAPYERPALS KGFLLPEAPA 70 80 90 100 110 120 RLPSFHTCVG ANDEKLTPKW YKDHGIELVL GTRVKSVDVR RKTLLSSTGE TISYKFLIIA 130 140 150 160 170 180 TGARALKLEE FGVEGSDAEN VCYLRDLADA NRLATVIQSS SNGNAVVIGG GYIGMECAAS 190 200 210 220 230 240 LVINKINVTM VFPEAHCMAR LFTPKIASLY EDYYRAKGVK FIKGTVLTSF EFDSNKKVTA 250 260 270 280 290 300 VNLKDGSHLP ADLVVVGIGI RPNTSLFEGQ LTIEKGGIKV NSRMQSSDSS VYAIGDVATF 310 320 330 340 350 360 PVKLFGEMRR LEHVDSARKS ARHAVSAIMD PIKTGDFDYL PFFYSRVFAF SWQFYGDPTG 370 380 390 400 410 420 DVVHFGEYED GKSFGAYWVK KGHLVGSFLE GGTKEEYETI SKATQLKPAV TIDLEELERE 430 440 450 460 470 480 GLGFAHTVVS QQKVPEVKDI PSAEMVKQSA SVVMIKKPLY VWHAATGVVV AASVAAFAFW YGRRRRRW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP000371 Genomic DNA. Translation: BAB02528.1. CP002686 Genomic DNA. Translation: AEE77367.1. AY039980 mRNA. Translation: AAK64157.1. AY133800 mRNA. Translation: AAM91734.1.
IPI	IPI00519389.
RefSeq	NP_189420.1. NM_113698.3.
UniGene	At.5731.
3D structure databases
ProteinModelPortal	Q9LK94.
SMR	Q9LK94. Positions 2-420.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LK94.
Proteomic databases
PRIDE	Q9LK94.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G27820.1; AT3G27820.1; AT3G27820.
GeneID	822402.
GenomeReviews	Gene locus AT3G27820 in contig BA000014_GR.
KEGG	ath:AT3G27820.
NMPDR	fig|3702.1.peg.15072.
Organism-specific databases
TAIR	At3g27820.
Phylogenomic databases
eggNOG	KOG1336.
GeneTree	EPGT00070000028875.
HOGENOM	HBG316689.
InParanoid	Q9LK94.
OMA	LCIISEE.
PhylomeDB	Q9LK94.
ProtClustDB	CLSN2684894.
Gene expression databases
ArrayExpress	Q9LK94.
Genevestigator	Q9LK94.
GermOnline	AT3G27820. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR001327. Pyr_OxRdtase_NAD-bd_dom. [Graphical view]
KO	K08232.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDAR2_ARATH

Accession

Primary (citable) accession number: Q9LK94

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	October 1, 2000
Last modified:	November 16, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program