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Protein

ABC transporter C family member 3

Gene

ABCC3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pump for glutathione S-conjugates. Mediates the transport of glutathione conjugates such as chlorodinitrobenzene-GS (DNB-GS), and of chlorophyll catabolites such as Bn-NCC-1. Transports also heavy metals such as cadmium (Cd).1 Publication

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Enzyme regulationi

Glutathione-conjugate transport is inhibited by decyl-glutathione and, to a lower extent, by GS-GS, but not by GSH. All transports are inhibited by vanadate.1 Publication

Kineticsi

  1. KM=100 µM for DNB-GS (at pH 7.4 and 25 degrees Celsius)1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi675 – 6828ATP 1PROSITE-ProRule annotation
    Nucleotide bindingi1303 – 13108ATP 2PROSITE-ProRule annotation

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G13080-MONOMER.
    ARA:GQT-1977-MONOMER.
    ARA:GQT-1978-MONOMER.
    ARA:GQT-1979-MONOMER.
    ReactomeiR-ATH-382556. ABC-family proteins mediated transport.

    Protein family/group databases

    TCDBi3.A.1.208.17. the atp-binding cassette (abc) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ABC transporter C family member 3 (EC:3.6.3.44)
    Short name:
    ABC transporter ABCC.3
    Short name:
    AtABCC3
    Alternative name(s):
    ATP-energized glutathione S-conjugate pump 3
    Glutathione S-conjugate-transporting ATPase 3
    Multidrug resistance-associated protein 3
    Gene namesi
    Name:ABCC3
    Synonyms:EST2, MRP3
    Ordered Locus Names:At3g13080
    ORF Names:MJG19.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 3

    Organism-specific databases

    TAIRiAT3G13080.

    Subcellular locationi

    • Membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei35 – 5521HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei83 – 10321HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei116 – 13621HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei153 – 17321HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei183 – 20321HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei325 – 34521HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei364 – 38623HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei439 – 45921HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei463 – 48321HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei551 – 57121HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei940 – 96021HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei987 – 100721HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei1077 – 109721HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei1181 – 120121HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei1205 – 122521HelicalPROSITE-ProRule annotationAdd
    BLAST

    GO - Cellular componenti

    • apoplast Source: TAIR
    • integral component of membrane Source: UniProtKB-KW
    • plant-type vacuole Source: TAIR
    • plasmodesma Source: TAIR
    • vacuolar membrane Source: TAIR
    • vacuole Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15141514ABC transporter C family member 3PRO_0000226074Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei904 – 9041PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9LK64.
    PRIDEiQ9LK64.

    Expressioni

    Tissue specificityi

    Ubiquitous.2 Publications

    Inductioni

    By 1-chloro-2,4-dinitrobenzene (CDNB), primisulfuron (PS), aminotriazole, benoxacor, oxabetrinil and IRL 1803, and, to a lower extent, by cloquintocet, fenchlorazol and fluorazol.2 Publications

    Gene expression databases

    ExpressionAtlasiQ9LK64. baseline and differential.
    GenevisibleiQ9LK64. AT.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT3G13080.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LK64.
    SMRiQ9LK64. Positions 313-932, 953-1501.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini325 – 606282ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini640 – 863224ABC transporter 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini950 – 1232283ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1271 – 1503233ABC transporter 2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1507 – 15126Poly-Ser

    Sequence similaritiesi

    Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
    Contains 2 ABC transporter domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG0054. Eukaryota.
    COG1132. LUCA.
    InParanoidiQ9LK64.
    OMAiRIGNFSW.
    PhylomeDBiQ9LK64.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00664. ABC_membrane. 2 hits.
    PF00005. ABC_tran. 2 hits.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF90123. SSF90123. 2 hits.
    PROSITEiPS50929. ABC_TM1F. 2 hits.
    PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q9LK64-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MDFLGSTTGS GTLAMLFSFS ESILPLDSRS FLLKPLFLRW LSGFLHSVLL
    60 70 80 90 100
    LVLFFSWVRK KIRGDSGVTE SLKDRRDFGF KSALFCSLAL SLLNLVLMSL
    110 120 130 140 150
    SGFYWYESGW LDNEQLVSSL GFLLGMVSWG VLSICLHRCR DCEHKKAPFL
    160 170 180 190 200
    LRLWLVFYLV VSCYSLVVDF VMYERRETVP VHLLVFDIVA FIAAVFLGYV
    210 220 230 240 250
    AVLKKDRSNS NGVLEEPLLN GGDSRVGGDD SVELNKTNGS GEATPYSRAG
    260 270 280 290 300
    ILSLLTFSWM SPLIDIGNKK TLDLEDVPQL HDTDSVVGLA PKFRSMLESP
    310 320 330 340 350
    DGGERSGVTT FKLIKALYFT AQWEILVTAF FAFIYTVASY VGPALIDTFV
    360 370 380 390 400
    QYLNGRRQYN HEGYVLVITF FAAKIVECLS QRHWFFRLQK VGIRMRSALV
    410 420 430 440 450
    AMIYEKGLTL SCQSKQGRTS GEIINFMTVD AERIGNFSWY MHDPWMVLLQ
    460 470 480 490 500
    VGLALWILYR NLGLASIAAL VATIIVMLIN FPFGRMQERF QEKLMEAKDS
    510 520 530 540 550
    RMKSTSEILR NMRILKLQGW EMKFLSKIFD LRKSEEGWLK KYVYNSAVIS
    560 570 580 590 600
    FVFWGAPTLV SVSTFGACIL LGIPLESGKI LSALATFRIL QEPIYNLPDT
    610 620 630 640 650
    ISMIVQTKVS LDRLASYLCL DNLQPDIVER LPKGSSDVAV EVINSTLSWD
    660 670 680 690 700
    VSSSNPTLKD INFKVFPGMK VAVCGTVGSG KSSLLSSLLG EVPKVSGSLK
    710 720 730 740 750
    VCGTKAYVAQ SPWIQSGKIE DNILFGKPME RERYDKVLEA CSLSKDLEIL
    760 770 780 790 800
    SFGDQTVIGE RGINLSGGQK QRIQIARALY QDADIYLFDD PFSAVDAHTG
    810 820 830 840 850
    SHLFKEVLLG LLCSKSVIYV THQVEFLPAA DLILVMKDGR ISQAGKYNDI
    860 870 880 890 900
    LNSGTDFMEL IGAHQEALAV VDSVDANSVS EKSALGQENV IVKDAIAVDE
    910 920 930 940 950
    KLESQDLKND KLESVEPQRQ IIQEEEREKG SVALDVYWKY ITLAYGGALV
    960 970 980 990 1000
    PFILLGQVLF QLLQIGSNYW MAWATPVSED VQAPVKLSTL MIVYVALAFG
    1010 1020 1030 1040 1050
    SSLCILLRAT LLVTAGYKTA TELFHKMHHC IFRSPMSFFD STPSGRIMSR
    1060 1070 1080 1090 1100
    ASTDQSAVDL ELPYQFGSVA ITVIQLIGII GVMSQVSWLV FLVFIPVVAA
    1110 1120 1130 1140 1150
    SIWYQRYYIA AARELSRLVG VCKAPLIQHF SETISGATTI RSFSQEFRFR
    1160 1170 1180 1190 1200
    SDNMRLSDGY SRPKFYTAGA MEWLCFRLDM LSSLTFVFSL VFLVSIPTGV
    1210 1220 1230 1240 1250
    IDPSLAGLAV TYGLSLNTLQ AWLIWTLCNL ENKIISVERI LQYASVPSEP
    1260 1270 1280 1290 1300
    PLVIESNRPE QSWPSRGEVE IRDLQVRYAP HMPLVLRGIT CTFKGGLRTG
    1310 1320 1330 1340 1350
    IVGRTGSGKS TLIQTLFRIV EPSAGEIRID GVNILTIGLH DLRLRLSIIP
    1360 1370 1380 1390 1400
    QDPTMFEGTM RSNLDPLEEY TDDQIWEALD KCQLGDEVRK KEQKLDSSVS
    1410 1420 1430 1440 1450
    ENGDNWSMGQ RQLVCLGRVL LKRSKILVLD EATASVDTAT DNLIQKTLRE
    1460 1470 1480 1490 1500
    HFSDCTVITI AHRISSVIDS DMVLLLSNGI IEEYDTPVRL LEDKSSSFSK
    1510
    LVAEYTSRSS SSFD
    Length:1,514
    Mass (Da):168,971
    Last modified:October 1, 2000 - v1
    Checksum:i455575FCD3DA5115
    GO
    Isoform 2 (identifier: Q9LK64-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1347-1372: SIIPQDPTMFEGTMRSNLDPLEEYTD → N

    Note: Derived from EST data. No experimental confirmation available.
    Show »
    Length:1,489
    Mass (Da):166,103
    Checksum:i217F67BF2FBC405D
    GO

    Sequence cautioni

    The sequence AAC49791.1 differs from that shown. Reason: Frameshift at positions 1159, 1169, 1186, 1189 and 1196. Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1347 – 137226SIIPQ…EEYTD → N in isoform 2. CuratedVSP_018099Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U92650 mRNA. Translation: AAC49791.1. Frameshift.
    AP000375 Genomic DNA. Translation: BAB01399.1.
    CP002686 Genomic DNA. Translation: AEE75291.1.
    CP002686 Genomic DNA. Translation: AEE75292.1.
    PIRiT52081.
    RefSeqiNP_187915.1. NM_112147.2. [Q9LK64-1]
    NP_850575.1. NM_180244.1. [Q9LK64-2]
    UniGeneiAt.20479.

    Genome annotation databases

    EnsemblPlantsiAT3G13080.1; AT3G13080.1; AT3G13080. [Q9LK64-1]
    GeneIDi820496.
    KEGGiath:AT3G13080.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U92650 mRNA. Translation: AAC49791.1. Frameshift.
    AP000375 Genomic DNA. Translation: BAB01399.1.
    CP002686 Genomic DNA. Translation: AEE75291.1.
    CP002686 Genomic DNA. Translation: AEE75292.1.
    PIRiT52081.
    RefSeqiNP_187915.1. NM_112147.2. [Q9LK64-1]
    NP_850575.1. NM_180244.1. [Q9LK64-2]
    UniGeneiAt.20479.

    3D structure databases

    ProteinModelPortaliQ9LK64.
    SMRiQ9LK64. Positions 313-932, 953-1501.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT3G13080.1.

    Protein family/group databases

    TCDBi3.A.1.208.17. the atp-binding cassette (abc) superfamily.

    Proteomic databases

    PaxDbiQ9LK64.
    PRIDEiQ9LK64.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT3G13080.1; AT3G13080.1; AT3G13080. [Q9LK64-1]
    GeneIDi820496.
    KEGGiath:AT3G13080.

    Organism-specific databases

    TAIRiAT3G13080.

    Phylogenomic databases

    eggNOGiKOG0054. Eukaryota.
    COG1132. LUCA.
    InParanoidiQ9LK64.
    OMAiRIGNFSW.
    PhylomeDBiQ9LK64.

    Enzyme and pathway databases

    BioCyciARA:AT3G13080-MONOMER.
    ARA:GQT-1977-MONOMER.
    ARA:GQT-1978-MONOMER.
    ARA:GQT-1979-MONOMER.
    ReactomeiR-ATH-382556. ABC-family proteins mediated transport.

    Miscellaneous databases

    PROiQ9LK64.

    Gene expression databases

    ExpressionAtlasiQ9LK64. baseline and differential.
    GenevisibleiQ9LK64. AT.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00664. ABC_membrane. 2 hits.
    PF00005. ABC_tran. 2 hits.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF90123. SSF90123. 2 hits.
    PROSITEiPS50929. ABC_TM1F. 2 hits.
    PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 2 hits.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Differential expression of genes coding for ABC transporters after treatment of Arabidopsis thaliana with xenobiotics."
      Tommasini R., Vogt E., Schmid J., Fromentau M., Amrhein N., Martinoia E.
      FEBS Lett. 411:206-210(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION.
      Strain: cv. Columbia.
    2. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
      DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Cloning and expression analyses of the AtMRP4, a novel MRP-like gene from Arabidopsis thaliana."
      Sanchez-Fernandez R., Ardiles-Diaz W., Van Montagu M., Inze D., May M.J.
      Mol. Gen. Genet. 258:655-662(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    5. "An ABC-transporter of Arabidopsis thaliana has both glutathione-conjugate and chlorophyll catabolite transport activity."
      Tommasini R., Vogt E., Fromenteau M., Hoertensteiner S., Matile P., Amrhein N., Martinoia E.
      Plant J. 13:773-780(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    6. "The Arabidopsis thaliana ABC protein superfamily, a complete inventory."
      Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A.
      J. Biol. Chem. 276:30231-30244(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    7. "Multifunctionality of plant ABC transporters -- more than just detoxifiers."
      Martinoia E., Klein M., Geisler M., Bovet L., Forestier C., Kolukisaoglu H.U., Mueller-Roeber B., Schulz B.
      Planta 214:345-355(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    8. "Family business: the multidrug-resistance related protein (MRP) ABC transporter genes in Arabidopsis thaliana."
      Kolukisaoglu U.H., Bovet L., Klein M., Eggmann T., Geisler M., Wanke D., Martinoia E., Schulz B.
      Planta 216:107-119(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    9. Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiAB3C_ARATH
    AccessioniPrimary (citable) accession number: Q9LK64
    Secondary accession number(s): O24510, Q3EB73
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 7, 2006
    Last sequence update: October 1, 2000
    Last modified: January 20, 2016
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.