Reviewed, UniProtKB/Swiss-Prot Q9LK64 (AB3C_ARATH)

Names and origin

Protein names

Recommended name:
    ABC transporter C family member 3
      Short name=ABC transporter ABCC.3
      Short name=AtABCC3
    EC=3.6.3.44
Alternative name(s):
    Multidrug resistance-associated protein 3
    Glutathione S-conjugate-transporting ATPase 3
    ATP-energized glutathione S-conjugate pump 3

Gene names

Name:	ABCC3
Synonyms:	EST2, MRP3
Ordered Locus Names:	At3g13080
ORF Names:	MJG19.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1514 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Pump for glutathione S-conjugates. Mediates the transport of glutathione conjugates such as chlorodinitrobenzene-GS (DNB-GS), and of chlorophyll catabolites such as Bn-NCC-1. Transports also heavy metals such as cadmium (Cd). Ref.4
Catalytic activity	ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).
Enzyme regulation	Glutathione-conjugate transport is inhibited by decyl-glutathione and, to a lower extent, by GS-GS, but not by GSH. All transports are inhibited by vanadate. Ref.4
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Ubiquitous. Ref.4 Ref.7
Induction	By 1-chloro-2,4-dinitrobenzene (CDNB), primisulfuron (PS), aminotriazole, benoxacor, oxabetrinil and IRL 1803, and, to a lower extent, by cloquintocet, fenchlorazol and fluorazol. Ref.1 Ref.3
Sequence similarities	Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification] Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains.
Biophysicochemical properties	Kinetic parameters: KM=100 µM for DNB-GS (at pH 7.4 and 25 degrees Celsius)
Sequence caution	The sequence AAC49791.1 differs from that shown. Reason: Frameshift at positions 1159, 1169, 1186, 1189 and 1196.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from direct assay. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plant-type vacuole Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR vacuolar membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW chlorophyll catabolite transporter activity Ref.4 Inferred from direct assay. Source: TAIR glutathione S-conjugate-exporting ATPase activity Ref.4 Inferred from direct assay. Source: TAIR xenobiotic-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9LK64-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9LK64-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1347-1372: SIIPQDPTMFEGTMRSNLDPLEEYTD → N

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1514	1514	ABC transporter C family member 3		PRO_0000226074
Regions
	Transmembrane	35 – 55	21	Potential
	Transmembrane	83 – 103	21	Potential
	Transmembrane	116 – 136	21	Potential
	Transmembrane	153 – 173	21	Potential
	Transmembrane	183 – 203	21	Potential
	Transmembrane	325 – 345	21	Potential
	Transmembrane	364 – 386	23	Potential
	Transmembrane	439 – 459	21	Potential
	Transmembrane	463 – 483	21	Potential
	Transmembrane	551 – 571	21	Potential
	Transmembrane	940 – 960	21	Potential
	Transmembrane	987 – 1007	21	Potential
	Transmembrane	1077 – 1097	21	Potential
	Transmembrane	1181 – 1201	21	Potential
	Transmembrane	1205 – 1225	21	Potential
	Domain	325 – 606	282	ABC transmembrane type-1 1
	Domain	640 – 863	224	ABC transporter 1
	Domain	950 – 1232	283	ABC transmembrane type-1 2
	Domain	1271 – 1503	233	ABC transporter 2
	Nucleotide binding	675 – 682	8	ATP 1 Potential
	Nucleotide binding	1303 – 1310	8	ATP 2 Potential
	Compositional bias	1507 – 1512	6	Poly-Ser
Natural variations
	Alternative sequence	1347 – 1372	26	SIIPQ…EEYTD → N in isoform 2.		VSP_018099

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 455575FCD3DA5115	FASTA	1,514	168,971
	10 20 30 40 50 60 MDFLGSTTGS GTLAMLFSFS ESILPLDSRS FLLKPLFLRW LSGFLHSVLL LVLFFSWVRK 70 80 90 100 110 120 KIRGDSGVTE SLKDRRDFGF KSALFCSLAL SLLNLVLMSL SGFYWYESGW LDNEQLVSSL 130 140 150 160 170 180 GFLLGMVSWG VLSICLHRCR DCEHKKAPFL LRLWLVFYLV VSCYSLVVDF VMYERRETVP 190 200 210 220 230 240 VHLLVFDIVA FIAAVFLGYV AVLKKDRSNS NGVLEEPLLN GGDSRVGGDD SVELNKTNGS 250 260 270 280 290 300 GEATPYSRAG ILSLLTFSWM SPLIDIGNKK TLDLEDVPQL HDTDSVVGLA PKFRSMLESP 310 320 330 340 350 360 DGGERSGVTT FKLIKALYFT AQWEILVTAF FAFIYTVASY VGPALIDTFV QYLNGRRQYN 370 380 390 400 410 420 HEGYVLVITF FAAKIVECLS QRHWFFRLQK VGIRMRSALV AMIYEKGLTL SCQSKQGRTS 430 440 450 460 470 480 GEIINFMTVD AERIGNFSWY MHDPWMVLLQ VGLALWILYR NLGLASIAAL VATIIVMLIN 490 500 510 520 530 540 FPFGRMQERF QEKLMEAKDS RMKSTSEILR NMRILKLQGW EMKFLSKIFD LRKSEEGWLK 550 560 570 580 590 600 KYVYNSAVIS FVFWGAPTLV SVSTFGACIL LGIPLESGKI LSALATFRIL QEPIYNLPDT 610 620 630 640 650 660 ISMIVQTKVS LDRLASYLCL DNLQPDIVER LPKGSSDVAV EVINSTLSWD VSSSNPTLKD 670 680 690 700 710 720 INFKVFPGMK VAVCGTVGSG KSSLLSSLLG EVPKVSGSLK VCGTKAYVAQ SPWIQSGKIE 730 740 750 760 770 780 DNILFGKPME RERYDKVLEA CSLSKDLEIL SFGDQTVIGE RGINLSGGQK QRIQIARALY 790 800 810 820 830 840 QDADIYLFDD PFSAVDAHTG SHLFKEVLLG LLCSKSVIYV THQVEFLPAA DLILVMKDGR 850 860 870 880 890 900 ISQAGKYNDI LNSGTDFMEL IGAHQEALAV VDSVDANSVS EKSALGQENV IVKDAIAVDE 910 920 930 940 950 960 KLESQDLKND KLESVEPQRQ IIQEEEREKG SVALDVYWKY ITLAYGGALV PFILLGQVLF 970 980 990 1000 1010 1020 QLLQIGSNYW MAWATPVSED VQAPVKLSTL MIVYVALAFG SSLCILLRAT LLVTAGYKTA 1030 1040 1050 1060 1070 1080 TELFHKMHHC IFRSPMSFFD STPSGRIMSR ASTDQSAVDL ELPYQFGSVA ITVIQLIGII 1090 1100 1110 1120 1130 1140 GVMSQVSWLV FLVFIPVVAA SIWYQRYYIA AARELSRLVG VCKAPLIQHF SETISGATTI 1150 1160 1170 1180 1190 1200 RSFSQEFRFR SDNMRLSDGY SRPKFYTAGA MEWLCFRLDM LSSLTFVFSL VFLVSIPTGV 1210 1220 1230 1240 1250 1260 IDPSLAGLAV TYGLSLNTLQ AWLIWTLCNL ENKIISVERI LQYASVPSEP PLVIESNRPE 1270 1280 1290 1300 1310 1320 QSWPSRGEVE IRDLQVRYAP HMPLVLRGIT CTFKGGLRTG IVGRTGSGKS TLIQTLFRIV 1330 1340 1350 1360 1370 1380 EPSAGEIRID GVNILTIGLH DLRLRLSIIP QDPTMFEGTM RSNLDPLEEY TDDQIWEALD 1390 1400 1410 1420 1430 1440 KCQLGDEVRK KEQKLDSSVS ENGDNWSMGQ RQLVCLGRVL LKRSKILVLD EATASVDTAT 1450 1460 1470 1480 1490 1500 DNLIQKTLRE HFSDCTVITI AHRISSVIDS DMVLLLSNGI IEEYDTPVRL LEDKSSSFSK 1510 LVAEYTSRSS SSFD
	Isoform 2. Checksum: 217F67BF2FBC405D	FASTA	1,489	166,103
	10 20 30 40 50 60 MDFLGSTTGS GTLAMLFSFS ESILPLDSRS FLLKPLFLRW LSGFLHSVLL LVLFFSWVRK 70 80 90 100 110 120 KIRGDSGVTE SLKDRRDFGF KSALFCSLAL SLLNLVLMSL SGFYWYESGW LDNEQLVSSL 130 140 150 160 170 180 GFLLGMVSWG VLSICLHRCR DCEHKKAPFL LRLWLVFYLV VSCYSLVVDF VMYERRETVP 190 200 210 220 230 240 VHLLVFDIVA FIAAVFLGYV AVLKKDRSNS NGVLEEPLLN GGDSRVGGDD SVELNKTNGS 250 260 270 280 290 300 GEATPYSRAG ILSLLTFSWM SPLIDIGNKK TLDLEDVPQL HDTDSVVGLA PKFRSMLESP 310 320 330 340 350 360 DGGERSGVTT FKLIKALYFT AQWEILVTAF FAFIYTVASY VGPALIDTFV QYLNGRRQYN 370 380 390 400 410 420 HEGYVLVITF FAAKIVECLS QRHWFFRLQK VGIRMRSALV AMIYEKGLTL SCQSKQGRTS 430 440 450 460 470 480 GEIINFMTVD AERIGNFSWY MHDPWMVLLQ VGLALWILYR NLGLASIAAL VATIIVMLIN 490 500 510 520 530 540 FPFGRMQERF QEKLMEAKDS RMKSTSEILR NMRILKLQGW EMKFLSKIFD LRKSEEGWLK 550 560 570 580 590 600 KYVYNSAVIS FVFWGAPTLV SVSTFGACIL LGIPLESGKI LSALATFRIL QEPIYNLPDT 610 620 630 640 650 660 ISMIVQTKVS LDRLASYLCL DNLQPDIVER LPKGSSDVAV EVINSTLSWD VSSSNPTLKD 670 680 690 700 710 720 INFKVFPGMK VAVCGTVGSG KSSLLSSLLG EVPKVSGSLK VCGTKAYVAQ SPWIQSGKIE 730 740 750 760 770 780 DNILFGKPME RERYDKVLEA CSLSKDLEIL SFGDQTVIGE RGINLSGGQK QRIQIARALY 790 800 810 820 830 840 QDADIYLFDD PFSAVDAHTG SHLFKEVLLG LLCSKSVIYV THQVEFLPAA DLILVMKDGR 850 860 870 880 890 900 ISQAGKYNDI LNSGTDFMEL IGAHQEALAV VDSVDANSVS EKSALGQENV IVKDAIAVDE 910 920 930 940 950 960 KLESQDLKND KLESVEPQRQ IIQEEEREKG SVALDVYWKY ITLAYGGALV PFILLGQVLF 970 980 990 1000 1010 1020 QLLQIGSNYW MAWATPVSED VQAPVKLSTL MIVYVALAFG SSLCILLRAT LLVTAGYKTA 1030 1040 1050 1060 1070 1080 TELFHKMHHC IFRSPMSFFD STPSGRIMSR ASTDQSAVDL ELPYQFGSVA ITVIQLIGII 1090 1100 1110 1120 1130 1140 GVMSQVSWLV FLVFIPVVAA SIWYQRYYIA AARELSRLVG VCKAPLIQHF SETISGATTI 1150 1160 1170 1180 1190 1200 RSFSQEFRFR SDNMRLSDGY SRPKFYTAGA MEWLCFRLDM LSSLTFVFSL VFLVSIPTGV 1210 1220 1230 1240 1250 1260 IDPSLAGLAV TYGLSLNTLQ AWLIWTLCNL ENKIISVERI LQYASVPSEP PLVIESNRPE 1270 1280 1290 1300 1310 1320 QSWPSRGEVE IRDLQVRYAP HMPLVLRGIT CTFKGGLRTG IVGRTGSGKS TLIQTLFRIV 1330 1340 1350 1360 1370 1380 EPSAGEIRID GVNILTIGLH DLRLRLNDQI WEALDKCQLG DEVRKKEQKL DSSVSENGDN 1390 1400 1410 1420 1430 1440 WSMGQRQLVC LGRVLLKRSK ILVLDEATAS VDTATDNLIQ KTLREHFSDC TVITIAHRIS 1450 1460 1470 1480 SVIDSDMVLL LSNGIIEEYD TPVRLLEDKS SSFSKLVAEY TSRSSSSFD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential expression of genes coding for ABC transporters after treatment of Arabidopsis thaliana with xenobiotics." Tommasini R., Vogt E., Schmid J., Fromentau M., Amrhein N., Martinoia E. FEBS Lett. 411:206-210(1997) [PubMed: 9271206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Cloning and expression analyses of the AtMRP4, a novel MRP-like gene from Arabidopsis thaliana." Sanchez-Fernandez R., Ardiles-Diaz W., Van Montagu M., Inze D., May M.J. Mol. Gen. Genet. 258:655-662(1998) [PubMed: 9671034] [Abstract] Cited for: INDUCTION.
[4]	"An ABC-transporter of Arabidopsis thaliana has both glutathione-conjugate and chlorophyll catabolite transport activity." Tommasini R., Vogt E., Fromenteau M., Hoertensteiner S., Matile P., Amrhein N., Martinoia E. Plant J. 13:773-780(1998) [PubMed: 9681016] [Abstract] Cited for: FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[5]	"The Arabidopsis thaliana ABC protein superfamily, a complete inventory." Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A. J. Biol. Chem. 276:30231-30244(2001) [PubMed: 11346655] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"Multifunctionality of plant ABC transporters -- more than just detoxifiers." Martinoia E., Klein M., Geisler M., Bovet L., Forestier C., Kolukisaoglu H.U., Mueller-Roeber B., Schulz B. Planta 214:345-355(2002) [PubMed: 11855639] [Abstract] Cited for: GENE FAMILY.
[7]	"Family business: the multidrug-resistance related protein (MRP) ABC transporter genes in Arabidopsis thaliana." Kolukisaoglu U.H., Bovet L., Klein M., Eggmann T., Geisler M., Wanke D., Martinoia E., Schulz B. Planta 216:107-119(2002) [PubMed: 12430019] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Plant ABC proteins - a unified nomenclature and updated inventory." Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M., Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A., Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L. Trends Plant Sci. 13:151-159(2008) [PubMed: 18299247] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U92650 mRNA. Translation: AAC49791.1. Frameshift. AP000375 Genomic DNA. Translation: BAB01399.1.
IPI	IPI00520797. IPI00528825.
PIR	T52081.
RefSeq	NP_187915.1. NP_850575.1.
UniGene	At.20479
3D structure databases
HSSP	HSSP built from PDB template 1R0W based on UniProtKB P26361.
ModBase	Search...
Protein family/group databases
TCDB	3.A.1.208.17. ATP-binding cassette (ABC) superfamily.
Proteomic databases
PRIDE	Q9LK64.
Genome annotation databases
GeneID	820496.
GenomeReviews	Gene locus AT3G13080 in contig BA000014_GR.
KEGG	ath:AT3G13080.
NMPDR	fig|3702.1.peg.13396.
Organism-specific databases
TAIR	At3g13080.
Phylogenomic databases
OMA	NLPDTIS.
Enzyme and pathway databases
BRENDA	3.6.3.44. 302.
Gene expression databases
Genevestigator	Q9LK64.
Family and domain databases
InterPro	IPR001140. ABC_TM_transpt. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00664. ABC_membrane. 2 hits. PF00005. ABC_tran. 2 hits. [Graphical view]
ProDom	PD000006. ABC_transporter. 2 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00382. AAA. 2 hits. [Graphical view]
PROSITE	PS50929. ABC_TM1F. 2 hits. PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AB3C_ARATH

Accession

Primary (citable) accession number: Q9LK64
Secondary accession number(s): O24510, Q3EB73

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)