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Protein

Adenosylhomocysteinase 2

Gene

SAHH2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.By similarity

Miscellaneous

In contrast to SAHH1, a mutation in the C-terminus of this protein is not sufficient to abolish transcriptional gene silencing.

Catalytic activityi

S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine.

Cofactori

NAD+By similarity

Pathwayi: L-homocysteine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine.
Proteins known to be involved in this subpathway in this organism are:
  1. Adenosylhomocysteinase (HOG1), Adenosylhomocysteinase, Adenosylhomocysteinase (HOG1), Adenosylhomocysteinase 2 (SAHH2), Adenosylhomocysteinase 1 (SAHH1), Adenosylhomocysteinase (HOG1), Adenosylhomocysteinase (AXX17_At4g15900), Adenosylhomocysteinase (AXX17_At3g25710), Adenosylhomocysteinase (HOG1)
This subpathway is part of the pathway L-homocysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine, the pathway L-homocysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64SubstrateBy similarity1
Binding sitei139SubstrateBy similarity1
Binding sitei205SubstrateBy similarity1
Binding sitei235SubstrateBy similarity1
Binding sitei239SubstrateBy similarity1
Binding sitei240NADBy similarity1
Binding sitei292NADBy similarity1
Binding sitei327NADBy similarity1
Binding sitei397NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi206 – 208NADBy similarity3
Nucleotide bindingi269 – 274NADBy similarity6
Nucleotide bindingi348 – 350NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processOne-carbon metabolism
LigandNAD

Enzyme and pathway databases

BioCyciARA:AT3G23810-MONOMER.
ReactomeiR-ATH-156581. Methylation.
R-ATH-1614635. Sulfur amino acid metabolism.
UniPathwayiUPA00314; UER00076.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylhomocysteinase 2 (EC:3.3.1.1)
Short name:
AdoHcyase 2
Alternative name(s):
S-adenosyl-L-homocysteine hydrolase 1
SAH hydrolase 2
Gene namesi
Name:SAHH2
Ordered Locus Names:At3g23810
ORF Names:MYM9.16, MYM9_15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G23810.
TAIRilocus:2095193. AT3G23810.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001169211 – 485Adenosylhomocysteinase 2Add BLAST485

Proteomic databases

PaxDbiQ9LK36.
PRIDEiQ9LK36.

PTM databases

iPTMnetiQ9LK36.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LK36. baseline and differential.
GenevisibleiQ9LK36. AT.

Interactioni

Protein-protein interaction databases

BioGridi7296. 2 interactors.
STRINGi3702.AT3G23810.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LK36.
SMRiQ9LK36.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the adenosylhomocysteinase family.Curated

Phylogenomic databases

eggNOGiKOG1370. Eukaryota.
COG0499. LUCA.
HOGENOMiHOG000227986.
InParanoidiQ9LK36.
KOiK01251.
OMAiVSCVTEF.
OrthoDBiEOG093607MR.
PhylomeDBiQ9LK36.

Family and domain databases

CDDicd00401. SAHH. 1 hit.
HAMAPiMF_00563. AdoHcyase. 1 hit.
InterProiView protein in InterPro
IPR034373. Adenosylhomocysteinase.
IPR000043. Adenosylhomocysteinase-like.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR036291. NAD(P)-bd_dom_sf.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
PANTHERiPTHR23420. PTHR23420. 1 hit.
PfamiView protein in Pfam
PF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
PIRSFiPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTiView protein in SMART
SM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00936. ahcY. 1 hit.
PROSITEiView protein in PROSITE
PS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9LK36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLVEKTSS GREYKVKDMS QADFGRLEIE LAEVEMPGLV SCVTEFGPSQ
60 70 80 90 100
PLKGARITGS LHMTIQTAVL IETLTALGAE VRWCSCNIFS TQDHAAAAIA
110 120 130 140 150
RDSAAVFAWK GETLQEYWWC TERALDWGPG GGPDLIVDDG GDATLLIHEG
160 170 180 190 200
VKAEEIFAKN GTFPDPTSTD NPEFQIVLSI IKDGLQVDPK KYHKMKERLV
210 220 230 240 250
GVSEETTTGV KRLYQMQETG ALLFPAINVN DSVTKSKFDN LYGCRHSLPD
260 270 280 290 300
GLMRATDVMI AGKVAVICGY GDVGKGCAAA MKTAGARVIV TEIDPICALQ
310 320 330 340 350
ALMEGLQVLT LEDVVSEADI FCTTTGNKDI IMVDHMRKMK NNAIVCNIGH
360 370 380 390 400
FDNEIDMLGL ETYPGVKRIT IKPQTDRWVF PDTNSGIIVL AEGRLMNLGC
410 420 430 440 450
ATGHPSFVMS CSFTNQVIAQ LELWNEKSSG KYEKKVYVLP KHLDEKVAAL
460 470 480
HLGKLGARLT KLTKDQSDYV SIPVEGPYKP VHYRY
Length:485
Mass (Da):53,159
Last modified:October 1, 2000 - v1
Checksum:i876079E944DC3732
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44T → A in AAL16259 (PubMed:14593172).Curated1
Sequence conflicti155E → A in AAM19782 (PubMed:14593172).Curated1
Sequence conflicti442H → R in AAK92718 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000377 Genomic DNA. Translation: BAB01858.1.
CP002686 Genomic DNA. Translation: AEE76817.1.
AY059888 mRNA. Translation: AAL24370.1.
AY093385 mRNA. Translation: AAM13384.1.
AY150471 mRNA. Translation: AAN12996.1.
AF428329 mRNA. Translation: AAL16259.1.
AY094404 mRNA. Translation: AAM19782.1.
AY050783 mRNA. Translation: AAK92718.1.
RefSeqiNP_189023.1. NM_113286.3.
UniGeneiAt.24845.
At.67005.

Genome annotation databases

EnsemblPlantsiAT3G23810.1; AT3G23810.1; AT3G23810.
GeneIDi821964.
GrameneiAT3G23810.1; AT3G23810.1; AT3G23810.
KEGGiath:AT3G23810.

Similar proteinsi

Entry informationi

Entry nameiSAHH2_ARATH
AccessioniPrimary (citable) accession number: Q9LK36
Secondary accession number(s): Q8LPS8, Q944K5, Q949Z9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: October 1, 2000
Last modified: October 25, 2017
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families