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Q9LK23

- G6PD5_ARATH

UniProt

Q9LK23 - G6PD5_ARATH

Protein

Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1

Gene

ACG9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.

    Catalytic activityi

    D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

    Enzyme regulationi

    Regulated by metabolites.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei73 – 731NADPBy similarity
    Binding sitei183 – 1831NADP; via carbonyl oxygenBy similarity
    Binding sitei183 – 1831SubstrateBy similarity
    Binding sitei251 – 2511SubstrateBy similarity
    Binding sitei270 – 2701SubstrateBy similarity
    Active sitei275 – 2751Proton acceptorBy similarity
    Binding sitei361 – 3611SubstrateBy similarity
    Binding sitei397 – 3971SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi38 – 458NADPBy similarity

    GO - Molecular functioni

    1. glucose-6-phosphate dehydrogenase activity Source: TAIR
    2. NADP binding Source: InterPro

    GO - Biological processi

    1. glucose metabolic process Source: TAIR
    2. pentose-phosphate shunt, oxidative branch Source: TAIR
    3. response to cadmium ion Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciARA:GQT-1236-MONOMER.
    ARA:GQT-1237-MONOMER.
    UniPathwayiUPA00115; UER00408.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1 (EC:1.1.1.49)
    Alternative name(s):
    G6PDH5
    Short name:
    G6PD5
    Gene namesi
    Name:ACG9
    Ordered Locus Names:At3g27300
    ORF Names:K17E12.12
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G27300.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. cytosol Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 516516Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1PRO_0000068097Add
    BLAST

    Proteomic databases

    PaxDbiQ9LK23.
    PRIDEiQ9LK23.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9LK23.
    GenevestigatoriQ9LK23.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LK23.
    SMRiQ9LK23. Positions 24-506.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni213 – 2175Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0364.
    HOGENOMiHOG000046192.
    InParanoidiQ9LK23.
    KOiK00036.
    OMAiVVVMYVA.
    PhylomeDBiQ9LK23.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00966. G6PD.
    InterProiIPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23429. PTHR23429. 1 hit.
    PfamiPF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000110. G6PD. 1 hit.
    PRINTSiPR00079. G6PDHDRGNASE.
    TIGRFAMsiTIGR00871. zwf. 1 hit.
    PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9LK23-1 [UniParc]FASTAAdd to Basket

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    MGSGQWHMEK RSTLKNDSFV KEYNPVTETG SLSIIVLGAS GDLAKKKTFP    50
    ALFNLFHQGF LNPDEVHIFG YARSKITDEE LRDKIRGYLV DEKNASKKTE 100
    ALSKFLKLIK YVSGPYDSEE GFKRLDKAIL EHEISKKTAE GSSRRLFYLA 150
    LPPSVYPPVS KMIKAWCTNK SDLGGWTRIV VEKPFGKDLE SAEQLSSQIG 200
    ALFEEPQIYR IDHYLGKELV QNMLVLRFAN RLFLPLWNRD NIANVQIVFR 250
    EDFGTEGRGG YFDEYGIIRD IIQNHLLQVL CLVAMEKPIS LKPEHIRDEK 300
    VKVLQSVIPI KDEEVVLGQY EGYRDDPTVP NDSNTPTFAT TILRINNERW 350
    EGVPFILKAG KAMSSKKADI RIQFKDVPGD IFKCQNQGRN EFVIRLQPSE 400
    AMYMKLTVKQ PGLEMQTVQS ELDLSYKQRY QDVSIPEAYE RLILDTIRGD 450
    QQHFVRRDEL KAAWEIFTPL LHRIDKGEVK SVPYKQGSRG PAEADQLLKK 500
    AGYMQTHGYI WIPPTL 516
    Length:516
    Mass (Da):59,157
    Last modified:October 1, 2000 - v1
    Checksum:i407E42590EFC20E2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 261V → I in CAB52674. (PubMed:10437832)Curated
    Sequence conflicti376 – 3761D → G in AAL57688. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ010970 mRNA. Translation: CAB52674.1.
    AP000381 Genomic DNA. Translation: BAB02125.1.
    CP002686 Genomic DNA. Translation: AEE77290.1.
    CP002686 Genomic DNA. Translation: AEE77291.1.
    CP002686 Genomic DNA. Translation: AEE77292.1.
    AY065054 mRNA. Translation: AAL57688.1.
    PIRiT52611.
    RefSeqiNP_001030780.1. NM_001035703.2.
    NP_001078214.1. NM_001084745.1.
    NP_189366.1. NM_113644.4.
    UniGeneiAt.25212.

    Genome annotation databases

    EnsemblPlantsiAT3G27300.1; AT3G27300.1; AT3G27300.
    AT3G27300.2; AT3G27300.2; AT3G27300.
    AT3G27300.3; AT3G27300.3; AT3G27300.
    GeneIDi822349.
    KEGGiath:AT3G27300.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ010970 mRNA. Translation: CAB52674.1 .
    AP000381 Genomic DNA. Translation: BAB02125.1 .
    CP002686 Genomic DNA. Translation: AEE77290.1 .
    CP002686 Genomic DNA. Translation: AEE77291.1 .
    CP002686 Genomic DNA. Translation: AEE77292.1 .
    AY065054 mRNA. Translation: AAL57688.1 .
    PIRi T52611.
    RefSeqi NP_001030780.1. NM_001035703.2.
    NP_001078214.1. NM_001084745.1.
    NP_189366.1. NM_113644.4.
    UniGenei At.25212.

    3D structure databases

    ProteinModelPortali Q9LK23.
    SMRi Q9LK23. Positions 24-506.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q9LK23.
    PRIDEi Q9LK23.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G27300.1 ; AT3G27300.1 ; AT3G27300 .
    AT3G27300.2 ; AT3G27300.2 ; AT3G27300 .
    AT3G27300.3 ; AT3G27300.3 ; AT3G27300 .
    GeneIDi 822349.
    KEGGi ath:AT3G27300.

    Organism-specific databases

    TAIRi AT3G27300.

    Phylogenomic databases

    eggNOGi COG0364.
    HOGENOMi HOG000046192.
    InParanoidi Q9LK23.
    KOi K00036.
    OMAi VVVMYVA.
    PhylomeDBi Q9LK23.

    Enzyme and pathway databases

    UniPathwayi UPA00115 ; UER00408 .
    BioCyci ARA:GQT-1236-MONOMER.
    ARA:GQT-1237-MONOMER.

    Gene expression databases

    ArrayExpressi Q9LK23.
    Genevestigatori Q9LK23.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    HAMAPi MF_00966. G6PD.
    InterProi IPR001282. G6P_DH.
    IPR019796. G6P_DH_AS.
    IPR022675. G6P_DH_C.
    IPR022674. G6P_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23429. PTHR23429. 1 hit.
    Pfami PF02781. G6PD_C. 1 hit.
    PF00479. G6PD_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000110. G6PD. 1 hit.
    PRINTSi PR00079. G6PDHDRGNASE.
    TIGRFAMsi TIGR00871. zwf. 1 hit.
    PROSITEi PS00069. G6P_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evidence for functional convergence of redox regulation in G6PDH isoforms of cyanobacteria and higher plants."
      Wendt U.K., Hauschild R., Lange C., Pietersma M., Wenderoth I., von Schaewen A.
      Plant Mol. Biol. 40:487-494(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
      DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiG6PD5_ARATH
    AccessioniPrimary (citable) accession number: Q9LK23
    Secondary accession number(s): Q8VZD0, Q9SUK0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2002
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3