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Q9LK23 (G6PD5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1

EC=1.1.1.49
Alternative name(s):
G6PDH5
Short name=G6PD5
Gene names
Name:ACG9
Ordered Locus Names:At3g27300
ORF Names:K17E12.12
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length516 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division. HAMAP-Rule MF_00966

Catalytic activity

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH. HAMAP-Rule MF_00966

Enzyme regulation

Regulated by metabolites. HAMAP-Rule MF_00966

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. HAMAP-Rule MF_00966

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00966

Subcellular location

Cytoplasm HAMAP-Rule MF_00966.

Miscellaneous

There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

Sequence similarities

Belongs to the glucose-6-phosphate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 516516Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1 HAMAP-Rule MF_00966
PRO_0000068097

Regions

Nucleotide binding38 – 458NADP By similarity
Region213 – 2175Substrate binding By similarity

Sites

Active site2751Proton acceptor By similarity
Binding site731NADP By similarity
Binding site1831NADP; via carbonyl oxygen By similarity
Binding site1831Substrate By similarity
Binding site2511Substrate By similarity
Binding site2701Substrate By similarity
Binding site3611Substrate By similarity
Binding site3971Substrate By similarity

Experimental info

Sequence conflict261V → I in CAB52674. Ref.1
Sequence conflict3761D → G in AAL57688. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9LK23 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 407E42590EFC20E2

FASTA51659,157
        10         20         30         40         50         60 
MGSGQWHMEK RSTLKNDSFV KEYNPVTETG SLSIIVLGAS GDLAKKKTFP ALFNLFHQGF 

        70         80         90        100        110        120 
LNPDEVHIFG YARSKITDEE LRDKIRGYLV DEKNASKKTE ALSKFLKLIK YVSGPYDSEE 

       130        140        150        160        170        180 
GFKRLDKAIL EHEISKKTAE GSSRRLFYLA LPPSVYPPVS KMIKAWCTNK SDLGGWTRIV 

       190        200        210        220        230        240 
VEKPFGKDLE SAEQLSSQIG ALFEEPQIYR IDHYLGKELV QNMLVLRFAN RLFLPLWNRD 

       250        260        270        280        290        300 
NIANVQIVFR EDFGTEGRGG YFDEYGIIRD IIQNHLLQVL CLVAMEKPIS LKPEHIRDEK 

       310        320        330        340        350        360 
VKVLQSVIPI KDEEVVLGQY EGYRDDPTVP NDSNTPTFAT TILRINNERW EGVPFILKAG 

       370        380        390        400        410        420 
KAMSSKKADI RIQFKDVPGD IFKCQNQGRN EFVIRLQPSE AMYMKLTVKQ PGLEMQTVQS 

       430        440        450        460        470        480 
ELDLSYKQRY QDVSIPEAYE RLILDTIRGD QQHFVRRDEL KAAWEIFTPL LHRIDKGEVK 

       490        500        510 
SVPYKQGSRG PAEADQLLKK AGYMQTHGYI WIPPTL 

« Hide

References

« Hide 'large scale' references
[1]"Evidence for functional convergence of redox regulation in G6PDH isoforms of cyanobacteria and higher plants."
Wendt U.K., Hauschild R., Lange C., Pietersma M., Wenderoth I., von Schaewen A.
Plant Mol. Biol. 40:487-494(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ010970 mRNA. Translation: CAB52674.1.
AP000381 Genomic DNA. Translation: BAB02125.1.
CP002686 Genomic DNA. Translation: AEE77290.1.
CP002686 Genomic DNA. Translation: AEE77291.1.
CP002686 Genomic DNA. Translation: AEE77292.1.
AY065054 mRNA. Translation: AAL57688.1.
PIRT52611.
RefSeqNP_001030780.1. NM_001035703.2.
NP_001078214.1. NM_001084745.1.
NP_189366.1. NM_113644.4.
UniGeneAt.25212.

3D structure databases

ProteinModelPortalQ9LK23.
SMRQ9LK23. Positions 24-506.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ9LK23.
PRIDEQ9LK23.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G27300.1; AT3G27300.1; AT3G27300.
AT3G27300.2; AT3G27300.2; AT3G27300.
AT3G27300.3; AT3G27300.3; AT3G27300.
GeneID822349.
KEGGath:AT3G27300.

Organism-specific databases

TAIRAT3G27300.

Phylogenomic databases

eggNOGCOG0364.
HOGENOMHOG000046192.
InParanoidQ9LK23.
KOK00036.
OMAYFRFANA.
PhylomeDBQ9LK23.
ProtClustDBPLN02539.

Enzyme and pathway databases

BioCycARA:GQT-1236-MONOMER.
ARA:GQT-1237-MONOMER.
UniPathwayUPA00115; UER00408.

Gene expression databases

ArrayExpressQ9LK23.
GenevestigatorQ9LK23.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00966. G6PD.
InterProIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23429. PTHR23429. 1 hit.
PfamPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFPIRSF000110. G6PD. 1 hit.
PRINTSPR00079. G6PDHDRGNASE.
TIGRFAMsTIGR00871. zwf. 1 hit.
PROSITEPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG6PD5_ARATH
AccessionPrimary (citable) accession number: Q9LK23
Secondary accession number(s): Q8VZD0, Q9SUK0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: October 1, 2000
Last modified: February 19, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names