Reviewed,
UniProtKB/Swiss-Prot Q9LK23 (G6PD5_ARATH)
Last modified
February 9, 2010.
Version 74.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1 EC=1.1.1.49 Alternative name(s): G6PDH5 Short name=G6PD5 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 516 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division. |
| Catalytic activity | D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH. |
| Enzyme regulation | Regulated by metabolites. |
| Pathway | |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | |
| Miscellaneous | There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana. |
| Sequence similarities | Belongs to the glucose-6-phosphate dehydrogenase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism Glucose metabolism |
| Cellular component | Cytoplasm |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt, oxidative branchInferred from direct assay. Source: TAIR response to cadmium ionInferred from expression pattern. Source: TAIR |
| Cellular component | chloroplast Inferred from direct assay. Source: TAIR |
| Molecular function | binding Inferred from electronic annotation. Source: InterPro glucose-6-phosphate dehydrogenase activityInferred from direct assay. Source: TAIR |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 516 | 516 | Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1 | PRO_0000068097 | |||||
Sites | |||||||||
| Active site | 275 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 40 | 1 | NADP By similarity | ||||||
| Binding site | 73 | 1 | NADP By similarity | ||||||
| Binding site | 213 | 1 | Substrate By similarity | ||||||
| Binding site | 217 | 1 | Substrate By similarity | ||||||
| Binding site | 366 | 1 | Substrate By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 26 | 1 | V → I in CAB52674. Ref.1 | ||||||
| Sequence conflict | 376 | 1 | D → G in AAL57688. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Evidence for functional convergence of redox regulation in G6PDH isoforms of cyanobacteria and higher plants." Wendt U.K., Hauschild R., Lange C., Pietersma M., Wenderoth I., von Schaewen A. Plant Mol. Biol. 40:487-494(1999) [PubMed: 10437832] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AJ010970 mRNA. Translation: CAB52674.1. AP000381 Genomic DNA. Translation: BAB02125.1. AY065054 mRNA. Translation: AAL57688.1. |
| IPI | IPI00544602. |
| PIR | T52611. |
| RefSeq | NP_001030780.1. NP_001078214.1. NP_189366.1. |
| UniGene | At.25212 |
3D structure databases | |
| SMR | Q9LK23. Positions 30-512. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q9LK23. |
Proteomic databases | |
| PRIDE | Q9LK23. |
Genome annotation databases | |
| GeneID | 822349. |
| GenomeReviews | Gene locus AT3G27300 in contig BA000014_GR. |
| KEGG | ath:AT3G27300. |
| NMPDR | fig|3702.1.peg.15019. |
Organism-specific databases | |
| TAIR | At3g27300. |
Phylogenomic databases | |
| HOGENOM | HBG322449. |
| InParanoid | Q9LK23. |
| OMA | AIPAIEP. |
| PhylomeDB | Q9LK23. |
Enzyme and pathway databases | |
| BRENDA | 1.1.1.49. 302. |
Gene expression databases | |
| ArrayExpress | Q9LK23. |
| Genevestigator | Q9LK23. |
| GermOnline | AT3G27300. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR001282. Glc-6-P_DH. IPR019796. Glc-6-P_DH_AS. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| PANTHER | PTHR23429. G6PDH. 1 hit. |
| Pfam | PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view] |
| PIRSF | PIRSF000110. G6PD. 1 hit. |
| PRINTS | PR00079. G6PDHDRGNASE. |
| TIGRFAMs | TIGR00871. zwf. 1 hit. |
| PROSITE | PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | G6PD5_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q9LK23 Secondary accession number(s): Q8VZD0, Q9SUK0 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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