Reviewed, UniProtKB/Swiss-Prot Q9LK23 (G6PD5_ARATH)

Names and origin

Protein names

Recommended name:
    Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1
    EC=1.1.1.49
Alternative name(s):
    G6PDH5
      Short name=G6PD5

Gene names

Name:	ACG9
Ordered Locus Names:	At3g27300
ORF Names:	K17E12.12

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	516 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.
Catalytic activity	D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH.
Enzyme regulation	Regulated by metabolites.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt, oxidative branch Inferred from direct assay. Source: TAIR response to cadmium ion Inferred from expression pattern. Source: TAIR
Cellular component	chloroplast Inferred from direct assay. Source: TAIR
Molecular function	binding Inferred from electronic annotation. Source: InterPro glucose-6-phosphate dehydrogenase activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 516	516	Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1		PRO_0000068097
Sites
	Active site	275	1	Proton acceptor By similarity
	Binding site	40	1	NADP By similarity
	Binding site	73	1	NADP By similarity
	Binding site	213	1	Substrate By similarity
	Binding site	217	1	Substrate By similarity
	Binding site	366	1	Substrate By similarity
Experimental info
	Sequence conflict	26	1	V → I in CAB52674. Ref.1
	Sequence conflict	376	1	D → G in AAL57688. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9LK23-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 407E42590EFC20E2	FASTA	516	59,157
	10 20 30 40 50 60 MGSGQWHMEK RSTLKNDSFV KEYNPVTETG SLSIIVLGAS GDLAKKKTFP ALFNLFHQGF 70 80 90 100 110 120 LNPDEVHIFG YARSKITDEE LRDKIRGYLV DEKNASKKTE ALSKFLKLIK YVSGPYDSEE 130 140 150 160 170 180 GFKRLDKAIL EHEISKKTAE GSSRRLFYLA LPPSVYPPVS KMIKAWCTNK SDLGGWTRIV 190 200 210 220 230 240 VEKPFGKDLE SAEQLSSQIG ALFEEPQIYR IDHYLGKELV QNMLVLRFAN RLFLPLWNRD 250 260 270 280 290 300 NIANVQIVFR EDFGTEGRGG YFDEYGIIRD IIQNHLLQVL CLVAMEKPIS LKPEHIRDEK 310 320 330 340 350 360 VKVLQSVIPI KDEEVVLGQY EGYRDDPTVP NDSNTPTFAT TILRINNERW EGVPFILKAG 370 380 390 400 410 420 KAMSSKKADI RIQFKDVPGD IFKCQNQGRN EFVIRLQPSE AMYMKLTVKQ PGLEMQTVQS 430 440 450 460 470 480 ELDLSYKQRY QDVSIPEAYE RLILDTIRGD QQHFVRRDEL KAAWEIFTPL LHRIDKGEVK 490 500 510 SVPYKQGSRG PAEADQLLKK AGYMQTHGYI WIPPTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for functional convergence of redox regulation in G6PDH isoforms of cyanobacteria and higher plants." Wendt U.K., Hauschild R., Lange C., Pietersma M., Wenderoth I., von Schaewen A. Plant Mol. Biol. 40:487-494(1999) [PubMed: 10437832] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ010970 mRNA. Translation: CAB52674.1. AP000381 Genomic DNA. Translation: BAB02125.1. AY065054 mRNA. Translation: AAL57688.1.
IPI	IPI00544602.
PIR	T52611.
RefSeq	NP_001030780.1. NP_001078214.1. NP_189366.1.
UniGene	At.25212
3D structure databases
SMR	Q9LK23. Positions 30-512.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LK23.
Proteomic databases
PRIDE	Q9LK23.
Genome annotation databases
GeneID	822349.
GenomeReviews	Gene locus AT3G27300 in contig BA000014_GR.
KEGG	ath:AT3G27300.
NMPDR	fig|3702.1.peg.15019.
Organism-specific databases
TAIR	At3g27300.
Phylogenomic databases
HOGENOM	HBG322449.
InParanoid	Q9LK23.
OMA	AIPAIEP.
PhylomeDB	Q9LK23.
Enzyme and pathway databases
BRENDA	1.1.1.49. 302.
Gene expression databases
ArrayExpress	Q9LK23.
Genevestigator	Q9LK23.
GermOnline	AT3G27300. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001282. Glc-6-P_DH. IPR019796. Glc-6-P_DH_AS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23429. G6PDH. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PIRSF	PIRSF000110. G6PD. 1 hit.
PRINTS	PR00079. G6PDHDRGNASE.
TIGRFAMs	TIGR00871. zwf. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PD5_ARATH

Accession

Primary (citable) accession number: Q9LK23
Secondary accession number(s): Q8VZD0, Q9SUK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)