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Reviewed, UniProtKB/Swiss-Prot Q9LK23 (G6PD5_ARATH)

Last modified November 4, 2008. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1
    EC=1.1.1.49
Alternative name(s):
    G6PDH5
      Short name=G6PD5
Gene names
Name: ACG9
Ordered Locus Names: At3g27300
ORF Names: K17E12.12
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length516 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.

Catalytic activity

D-glucose 6-phosphate + NADP(+) = D-glucono-1,5-lactone 6-phosphate + NADPH.

Enzyme regulation

Regulated by metabolites.

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm.

Miscellaneous

There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

Sequence similarities

Belongs to the glucose-6-phosphate dehydrogenase family.

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Ontologies

Keywords

   Biological processCarbohydrate metabolism
Glucose metabolism
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome

Gene Ontology (GO)

   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 516516Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1
PRO_0000068097

Sites

Active site2751Proton acceptor By similarity
Binding site401NADP By similarity
Binding site731NADP By similarity
Binding site2131Substrate By similarity
Binding site2171Substrate By similarity
Binding site3661Substrate By similarity

Experimental info

Sequence conflict261V → I in CAB52674. Ref.1
Sequence conflict3761D → G in AAL57688. Ref.3

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Sequences

Sequence LengthMass (Da)Tools
Q9LK23-1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 407E42590EFC20E2

FASTA51659,157
        10         20         30         40         50         60 
MGSGQWHMEK RSTLKNDSFV KEYNPVTETG SLSIIVLGAS GDLAKKKTFP ALFNLFHQGF 

        70         80         90        100        110        120 
LNPDEVHIFG YARSKITDEE LRDKIRGYLV DEKNASKKTE ALSKFLKLIK YVSGPYDSEE 

       130        140        150        160        170        180 
GFKRLDKAIL EHEISKKTAE GSSRRLFYLA LPPSVYPPVS KMIKAWCTNK SDLGGWTRIV 

       190        200        210        220        230        240 
VEKPFGKDLE SAEQLSSQIG ALFEEPQIYR IDHYLGKELV QNMLVLRFAN RLFLPLWNRD 

       250        260        270        280        290        300 
NIANVQIVFR EDFGTEGRGG YFDEYGIIRD IIQNHLLQVL CLVAMEKPIS LKPEHIRDEK 

       310        320        330        340        350        360 
VKVLQSVIPI KDEEVVLGQY EGYRDDPTVP NDSNTPTFAT TILRINNERW EGVPFILKAG 

       370        380        390        400        410        420 
KAMSSKKADI RIQFKDVPGD IFKCQNQGRN EFVIRLQPSE AMYMKLTVKQ PGLEMQTVQS 

       430        440        450        460        470        480 
ELDLSYKQRY QDVSIPEAYE RLILDTIRGD QQHFVRRDEL KAAWEIFTPL LHRIDKGEVK 

       490        500        510 
SVPYKQGSRG PAEADQLLKK AGYMQTHGYI WIPPTL

« Hide

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References

« Hide 'large scale' references
[1]"Evidence for functional convergence of redox regulation in G6PDH isoforms of cyanobacteria and higher plants."
Wendt U.K., Hauschild R., Lange C., Pietersma M., Wenderoth I., von Schaewen A.
Plant Mol. Biol. 40:487-494(1999) [PubMed: 10437832] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.

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Cross-references

Sequence databases

AJ010970 mRNA. Translation: CAB52674.1.
AP000381 Genomic DNA. Translation: BAB02125.1.
AY065054 mRNA. Translation: AAL57688.1.
PIRT52611.
RefSeqNP_001030780.1.
NP_001078214.1.
NP_189366.1.
UniGeneAt.25212

3D structure databases

HSSPHSSP built from PDB template 1QKI based on UniProtKB P11413.
ModBaseSearch...

Genome annotation databases

GeneID822349.
GenomeReviewsGene locus AT3G27300 in contig BA000014_GR.
KEGGath:AT3G27300.
NMPDRfig|3702.1.peg.15019.

Organism-specific databases

TAIRAt3g27300.

Gene expression databases

ArrayExpressQ9LK23.
GermOnlineAT3G27300. Arabidopsis thaliana.

Family and domain databases

InterProIPR001282. Glc-6-P_DHase.
IPR016040. NAD(P)-bd.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR23429. G6PDH. 1 hit.
PfamPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFPIRSF000110. G6PD. 1 hit.
PRINTSPR00079. G6PDHDRGNASE.
ProDomPD001129. G6PD. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00871. zwf. 1 hit.
PROSITEPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
BLOCKSSearch...
ProtoNetSearch...

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Entry information

Entry nameG6PD5_ARATH
AccessionPrimary (citable) accession number: Q9LK23
Secondary accession number(s): Q8VZD0, Q9SUK0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: October 1, 2000
Last modified: November 4, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents