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Protein

Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1

Gene

ACG9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.

Miscellaneous

There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Enzyme regulationi

Regulated by metabolites.

Pathwayi: pentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic (At1g24280), Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic (APG1), Glucose-6-phosphate 1-dehydrogenase (At3g27300), Glucose-6-phosphate 1-dehydrogenase (AXX17_At3g29760), Glucose-6-phosphate 1-dehydrogenase (G6PD4), Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic (At5g13110), Glucose-6-phosphate 1-dehydrogenase (AXX17_At1g25530), Glucose-6-phosphate 1-dehydrogenase (At5g35790), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g33080), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g33080), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g12520), Glucose-6-phosphate 1-dehydrogenase (G6PD5), Glucose-6-phosphate 1-dehydrogenase (G6PD5), Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 2 (ACG12), Glucose-6-phosphate 1-dehydrogenase (At1g09420), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g38480), Glucose-6-phosphate 1-dehydrogenase (AXX17_At1g09330), Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1 (ACG9), Glucose-6-phosphate 1-dehydrogenase (At5g40760), Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic (At1g09420)
  2. Probable 6-phosphogluconolactonase 5, chloroplastic (EMB2024), Probable 6-phosphogluconolactonase 3 (At5g24420), Probable 6-phosphogluconolactonase 4 (At5g24410), Probable 6-phosphogluconolactonase 1 (At1g13700), Probable 6-phosphogluconolactonase 2 (At3g49360)
  3. 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At5g39410), 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At3g01580), 6-phosphogluconate dehydrogenase, decarboxylating 2, chloroplastic (At5g41670), 6-phosphogluconate dehydrogenase, decarboxylating 3 (At3g02360), 6-phosphogluconate dehydrogenase, decarboxylating 1, chloroplastic (At1g64190), 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At1g57660)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73NADPBy similarity1
Binding sitei183NADP; via carbonyl oxygenBy similarity1
Binding sitei183SubstrateBy similarity1
Binding sitei251SubstrateBy similarity1
Binding sitei270SubstrateBy similarity1
Active sitei275Proton acceptorBy similarity1
Binding sitei361SubstrateBy similarity1
Binding sitei397SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 45NADPBy similarity8

GO - Molecular functioni

  • glucose-6-phosphate dehydrogenase activity Source: TAIR
  • NADP binding Source: InterPro

GO - Biological processi

  • glucose metabolic process Source: TAIR
  • pentose-phosphate shunt, oxidative branch Source: TAIR
  • response to cadmium ion Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism
LigandNADP

Enzyme and pathway databases

BRENDAi1.1.1.49 399
ReactomeiR-ATH-5628897 TP53 Regulates Metabolic Genes
R-ATH-71336 Pentose phosphate pathway (hexose monophosphate shunt)
UniPathwayiUPA00115; UER00408

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1 (EC:1.1.1.49)
Alternative name(s):
G6PDH5
Short name:
G6PD5
Gene namesi
Name:ACG9
Ordered Locus Names:At3g27300
ORF Names:K17E12.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G27300
TAIRilocus:2086558 AT3G27300

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000680971 – 516Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1Add BLAST516

Proteomic databases

PaxDbiQ9LK23
PRIDEiQ9LK23

PTM databases

iPTMnetiQ9LK23

Expressioni

Gene expression databases

ExpressionAtlasiQ9LK23 baseline and differential
GenevisibleiQ9LK23 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G27300.1

Structurei

3D structure databases

ProteinModelPortaliQ9LK23
SMRiQ9LK23
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni213 – 217Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0563 Eukaryota
COG0364 LUCA
HOGENOMiHOG000046192
InParanoidiQ9LK23
KOiK00036
OMAiCVEPINE
OrthoDBiEOG093607K8
PhylomeDBiQ9LK23

Family and domain databases

HAMAPiMF_00966 G6PD, 1 hit
InterProiView protein in InterPro
IPR001282 G6P_DH
IPR019796 G6P_DH_AS
IPR022675 G6P_DH_C
IPR022674 G6P_DH_NAD-bd
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR23429 PTHR23429, 1 hit
PfamiView protein in Pfam
PF02781 G6PD_C, 1 hit
PF00479 G6PD_N, 1 hit
PIRSFiPIRSF000110 G6PD, 1 hit
PRINTSiPR00079 G6PDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00871 zwf, 1 hit
PROSITEiView protein in PROSITE
PS00069 G6P_DEHYDROGENASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LK23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSGQWHMEK RSTLKNDSFV KEYNPVTETG SLSIIVLGAS GDLAKKKTFP
60 70 80 90 100
ALFNLFHQGF LNPDEVHIFG YARSKITDEE LRDKIRGYLV DEKNASKKTE
110 120 130 140 150
ALSKFLKLIK YVSGPYDSEE GFKRLDKAIL EHEISKKTAE GSSRRLFYLA
160 170 180 190 200
LPPSVYPPVS KMIKAWCTNK SDLGGWTRIV VEKPFGKDLE SAEQLSSQIG
210 220 230 240 250
ALFEEPQIYR IDHYLGKELV QNMLVLRFAN RLFLPLWNRD NIANVQIVFR
260 270 280 290 300
EDFGTEGRGG YFDEYGIIRD IIQNHLLQVL CLVAMEKPIS LKPEHIRDEK
310 320 330 340 350
VKVLQSVIPI KDEEVVLGQY EGYRDDPTVP NDSNTPTFAT TILRINNERW
360 370 380 390 400
EGVPFILKAG KAMSSKKADI RIQFKDVPGD IFKCQNQGRN EFVIRLQPSE
410 420 430 440 450
AMYMKLTVKQ PGLEMQTVQS ELDLSYKQRY QDVSIPEAYE RLILDTIRGD
460 470 480 490 500
QQHFVRRDEL KAAWEIFTPL LHRIDKGEVK SVPYKQGSRG PAEADQLLKK
510
AGYMQTHGYI WIPPTL
Length:516
Mass (Da):59,157
Last modified:October 1, 2000 - v1
Checksum:i407E42590EFC20E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26V → I in CAB52674 (PubMed:10437832).Curated1
Sequence conflicti376D → G in AAL57688 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010970 mRNA Translation: CAB52674.1
AP000381 Genomic DNA Translation: BAB02125.1
CP002686 Genomic DNA Translation: AEE77290.1
CP002686 Genomic DNA Translation: AEE77291.1
CP002686 Genomic DNA Translation: AEE77292.1
AY065054 mRNA Translation: AAL57688.1
PIRiT52611
RefSeqiNP_001030780.1, NM_001035703.3
NP_001078214.1, NM_001084745.1
NP_189366.1, NM_113644.5
UniGeneiAt.25212

Genome annotation databases

EnsemblPlantsiAT3G27300.1; AT3G27300.1; AT3G27300
AT3G27300.2; AT3G27300.2; AT3G27300
AT3G27300.3; AT3G27300.3; AT3G27300
AT3G27300.4; AT3G27300.4; AT3G27300
GeneIDi822349
GrameneiAT3G27300.1; AT3G27300.1; AT3G27300
AT3G27300.2; AT3G27300.2; AT3G27300
AT3G27300.3; AT3G27300.3; AT3G27300
AT3G27300.4; AT3G27300.4; AT3G27300
KEGGiath:AT3G27300

Similar proteinsi

Entry informationi

Entry nameiG6PD5_ARATH
AccessioniPrimary (citable) accession number: Q9LK23
Secondary accession number(s): Q8VZD0, Q9SUK0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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