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Protein

Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1

Gene

ACG9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis which are involved in membrane synthesis and cell division.

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Enzyme regulationi

Regulated by metabolites.

Pathwayi: pentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic (At1g24280), Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic (APG1), Glucose-6-phosphate 1-dehydrogenase (At3g27300), Glucose-6-phosphate 1-dehydrogenase (AXX17_At3g29760), Glucose-6-phosphate 1-dehydrogenase (G6PD4), Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic (At5g13110), Glucose-6-phosphate 1-dehydrogenase (AXX17_At1g25530), Glucose-6-phosphate 1-dehydrogenase (At5g35790), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g33080), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g33080), Glucose-6-phosphate 1-dehydrogenase (AXX17_At5g12520), Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 2 (ACG12), Glucose-6-phosphate 1-dehydrogenase (At1g09420), Glucose-6-phosphate 1-dehydrogenase (AXX17_At1g09330), Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1 (ACG9), Glucose-6-phosphate 1-dehydrogenase (At5g40760), Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic (At1g09420)
  2. Probable 6-phosphogluconolactonase 5, chloroplastic (EMB2024), Probable 6-phosphogluconolactonase 3 (At5g24420), Probable 6-phosphogluconolactonase 4 (At5g24410), Probable 6-phosphogluconolactonase 1 (At1g13700), Probable 6-phosphogluconolactonase 2 (At3g49360)
  3. 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At5g39410), 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At3g01580), 6-phosphogluconate dehydrogenase, decarboxylating 2, chloroplastic (At5g41670), 6-phosphogluconate dehydrogenase, decarboxylating 3 (At3g02360), 6-phosphogluconate dehydrogenase, decarboxylating 1, chloroplastic (At1g64190), 6-phosphogluconate dehydrogenase, decarboxylating (AXX17_At1g57660)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73NADPBy similarity1
Binding sitei183NADP; via carbonyl oxygenBy similarity1
Binding sitei183SubstrateBy similarity1
Binding sitei251SubstrateBy similarity1
Binding sitei270SubstrateBy similarity1
Active sitei275Proton acceptorBy similarity1
Binding sitei361SubstrateBy similarity1
Binding sitei397SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 45NADPBy similarity8

GO - Molecular functioni

  • glucose-6-phosphate dehydrogenase activity Source: TAIR
  • NADP binding Source: InterPro

GO - Biological processi

  • glucose metabolic process Source: TAIR
  • pentose-phosphate shunt, oxidative branch Source: TAIR
  • response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.1.1.49. 399.
ReactomeiR-ATH-5628897. TP53 Regulates Metabolic Genes.
R-ATH-71336. Pentose phosphate pathway (hexose monophosphate shunt).
UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1 (EC:1.1.1.49)
Alternative name(s):
G6PDH5
Short name:
G6PD5
Gene namesi
Name:ACG9
Ordered Locus Names:At3g27300
ORF Names:K17E12.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G27300.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000680971 – 516Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1Add BLAST516

Proteomic databases

PaxDbiQ9LK23.
PRIDEiQ9LK23.

PTM databases

iPTMnetiQ9LK23.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LK23. baseline and differential.
GenevisibleiQ9LK23. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G27300.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LK23.
SMRiQ9LK23.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni213 – 217Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0563. Eukaryota.
COG0364. LUCA.
HOGENOMiHOG000046192.
InParanoidiQ9LK23.
KOiK00036.
OMAiGGICANL.
OrthoDBiEOG093607K8.
PhylomeDBiQ9LK23.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD. 1 hit.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LK23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSGQWHMEK RSTLKNDSFV KEYNPVTETG SLSIIVLGAS GDLAKKKTFP
60 70 80 90 100
ALFNLFHQGF LNPDEVHIFG YARSKITDEE LRDKIRGYLV DEKNASKKTE
110 120 130 140 150
ALSKFLKLIK YVSGPYDSEE GFKRLDKAIL EHEISKKTAE GSSRRLFYLA
160 170 180 190 200
LPPSVYPPVS KMIKAWCTNK SDLGGWTRIV VEKPFGKDLE SAEQLSSQIG
210 220 230 240 250
ALFEEPQIYR IDHYLGKELV QNMLVLRFAN RLFLPLWNRD NIANVQIVFR
260 270 280 290 300
EDFGTEGRGG YFDEYGIIRD IIQNHLLQVL CLVAMEKPIS LKPEHIRDEK
310 320 330 340 350
VKVLQSVIPI KDEEVVLGQY EGYRDDPTVP NDSNTPTFAT TILRINNERW
360 370 380 390 400
EGVPFILKAG KAMSSKKADI RIQFKDVPGD IFKCQNQGRN EFVIRLQPSE
410 420 430 440 450
AMYMKLTVKQ PGLEMQTVQS ELDLSYKQRY QDVSIPEAYE RLILDTIRGD
460 470 480 490 500
QQHFVRRDEL KAAWEIFTPL LHRIDKGEVK SVPYKQGSRG PAEADQLLKK
510
AGYMQTHGYI WIPPTL
Length:516
Mass (Da):59,157
Last modified:October 1, 2000 - v1
Checksum:i407E42590EFC20E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26V → I in CAB52674 (PubMed:10437832).Curated1
Sequence conflicti376D → G in AAL57688 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010970 mRNA. Translation: CAB52674.1.
AP000381 Genomic DNA. Translation: BAB02125.1.
CP002686 Genomic DNA. Translation: AEE77290.1.
CP002686 Genomic DNA. Translation: AEE77291.1.
CP002686 Genomic DNA. Translation: AEE77292.1.
AY065054 mRNA. Translation: AAL57688.1.
PIRiT52611.
RefSeqiNP_001030780.1. NM_001035703.3.
NP_001078214.1. NM_001084745.1.
NP_189366.1. NM_113644.5.
UniGeneiAt.25212.

Genome annotation databases

EnsemblPlantsiAT3G27300.1; AT3G27300.1; AT3G27300.
AT3G27300.2; AT3G27300.2; AT3G27300.
AT3G27300.3; AT3G27300.3; AT3G27300.
GeneIDi822349.
GrameneiAT3G27300.1; AT3G27300.1; AT3G27300.
AT3G27300.2; AT3G27300.2; AT3G27300.
AT3G27300.3; AT3G27300.3; AT3G27300.
KEGGiath:AT3G27300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010970 mRNA. Translation: CAB52674.1.
AP000381 Genomic DNA. Translation: BAB02125.1.
CP002686 Genomic DNA. Translation: AEE77290.1.
CP002686 Genomic DNA. Translation: AEE77291.1.
CP002686 Genomic DNA. Translation: AEE77292.1.
AY065054 mRNA. Translation: AAL57688.1.
PIRiT52611.
RefSeqiNP_001030780.1. NM_001035703.3.
NP_001078214.1. NM_001084745.1.
NP_189366.1. NM_113644.5.
UniGeneiAt.25212.

3D structure databases

ProteinModelPortaliQ9LK23.
SMRiQ9LK23.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G27300.1.

PTM databases

iPTMnetiQ9LK23.

Proteomic databases

PaxDbiQ9LK23.
PRIDEiQ9LK23.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G27300.1; AT3G27300.1; AT3G27300.
AT3G27300.2; AT3G27300.2; AT3G27300.
AT3G27300.3; AT3G27300.3; AT3G27300.
GeneIDi822349.
GrameneiAT3G27300.1; AT3G27300.1; AT3G27300.
AT3G27300.2; AT3G27300.2; AT3G27300.
AT3G27300.3; AT3G27300.3; AT3G27300.
KEGGiath:AT3G27300.

Organism-specific databases

TAIRiAT3G27300.

Phylogenomic databases

eggNOGiKOG0563. Eukaryota.
COG0364. LUCA.
HOGENOMiHOG000046192.
InParanoidiQ9LK23.
KOiK00036.
OMAiGGICANL.
OrthoDBiEOG093607K8.
PhylomeDBiQ9LK23.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.
BRENDAi1.1.1.49. 399.
ReactomeiR-ATH-5628897. TP53 Regulates Metabolic Genes.
R-ATH-71336. Pentose phosphate pathway (hexose monophosphate shunt).

Miscellaneous databases

PROiQ9LK23.

Gene expression databases

ExpressionAtlasiQ9LK23. baseline and differential.
GenevisibleiQ9LK23. AT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD. 1 hit.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG6PD5_ARATH
AccessioniPrimary (citable) accession number: Q9LK23
Secondary accession number(s): Q8VZD0, Q9SUK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 6 glucose-6-phosphate 1-dehydrogenase genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.