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Protein

Ubiquitin-conjugating enzyme E2 19

Gene

UBC19

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Part of the anaphase-promoting complex (APC). May have a key function during cell cycle and be involved in cyclin B1 degradation.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei120 – 1201Glycyl thioester intermediate

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. ubiquitin-like protein transferase activity Source: GO_Central
  4. ubiquitin protein ligase activity Source: GO_Central
  5. ubiquitin protein ligase binding Source: GO_Central
  6. ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  1. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: GO_Central
  2. cell proliferation Source: TAIR
  3. protein polyubiquitination Source: GO_Central
  4. regulation of mitotic metaphase/anaphase transition Source: GO_Central
  5. ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G20060-MONOMER.
ARA:GQT-2648-MONOMER.
ReactomeiREACT_273097. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_284122. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_294737. Senescence-Associated Secretory Phenotype (SASP).
REACT_295391. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_299093. Regulation of APC/C activators between G1/S and early anaphase.
REACT_301780. APC/C:Cdc20 mediated degradation of Securin.
REACT_305944. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_314276. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
REACT_318090. Separation of Sister Chromatids.
REACT_319658. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_336371. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_353749. APC-Cdc20 mediated degradation of Nek2A.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 19 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 19
Gene namesi
Name:UBC19
Ordered Locus Names:At3g20060
ORF Names:MAL21.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G20060.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: TAIR
  2. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi120 – 1201C → A: Unable to form a complex with ubiquitin. 1 Publication
Mutagenesisi120 – 1201C → S: Formation of a stable oxygen-ester bond with ubiquitin. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181Ubiquitin-conjugating enzyme E2 19PRO_0000345185Add
BLAST

Proteomic databases

PaxDbiQ9LJZ5.

Expressioni

Tissue specificityi

Expressed in all tissues with cell division activities and in mature leaves.1 Publication

Developmental stagei

Expressed during the G1-S phases of the cell cycle.1 Publication

Inductioni

Not induced by heat shock, dark to light transition, proteasome inhibitor MG132 or geldanamycin.1 Publication

Gene expression databases

GenevestigatoriQ9LJZ5.

Interactioni

Protein-protein interaction databases

IntActiQ9LJZ5. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9LJZ5.
SMRiQ9LJZ5. Positions 37-176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233454.
InParanoidiQ9LJZ5.
KOiK06688.
OMAiNDYPFKS.
PhylomeDBiQ9LJZ5.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LJZ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATVNGYTGN TPAATTPAAT GSKQSAPPTK TVDSHSVLKR LQSELMGLMM
60 70 80 90 100
GADPGISAFP EEDNIFCWKG TITGSKDTVF EGTEYRLSLT FSNDYPFKSP
110 120 130 140 150
KVKFETCCFH PNVDLYGNIC LDILQDKWSS AYDVRTILLS IQSLLGEPNI
160 170 180
SSPLNNQAAQ LWSNQEEYRK MVEKLYKPLN A
Length:181
Mass (Da):19,999
Last modified:September 30, 2000 - v1
Checksum:iD4E5C632112D3796
GO
Isoform 2 (identifier: Q9LJZ5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.

Note: Derived from EST data.

Show »
Length:136
Mass (Da):15,431
Checksum:iC24F807C1FED09F4
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4545Missing in isoform 2. CuratedVSP_034927Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY127573 mRNA. Translation: AAM96886.1.
DQ027033 mRNA. Translation: AAY44859.1.
AP000383 Genomic DNA. Translation: BAB01863.1.
CP002686 Genomic DNA. Translation: AEE76325.1.
CP002686 Genomic DNA. Translation: AEE76326.1.
BT025536 mRNA. Translation: ABF58954.1.
RefSeqiNP_001078192.1. NM_001084723.1. [Q9LJZ5-2]
NP_566653.1. NM_112897.3. [Q9LJZ5-1]
UniGeneiAt.21408.

Genome annotation databases

EnsemblPlantsiAT3G20060.1; AT3G20060.1; AT3G20060. [Q9LJZ5-1]
GeneIDi821545.
KEGGiath:AT3G20060.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY127573 mRNA. Translation: AAM96886.1.
DQ027033 mRNA. Translation: AAY44859.1.
AP000383 Genomic DNA. Translation: BAB01863.1.
CP002686 Genomic DNA. Translation: AEE76325.1.
CP002686 Genomic DNA. Translation: AEE76326.1.
BT025536 mRNA. Translation: ABF58954.1.
RefSeqiNP_001078192.1. NM_001084723.1. [Q9LJZ5-2]
NP_566653.1. NM_112897.3. [Q9LJZ5-1]
UniGeneiAt.21408.

3D structure databases

ProteinModelPortaliQ9LJZ5.
SMRiQ9LJZ5. Positions 37-176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9LJZ5. 1 interaction.

Proteomic databases

PaxDbiQ9LJZ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G20060.1; AT3G20060.1; AT3G20060. [Q9LJZ5-1]
GeneIDi821545.
KEGGiath:AT3G20060.

Organism-specific databases

GeneFarmi2132. 224.
TAIRiAT3G20060.

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233454.
InParanoidiQ9LJZ5.
KOiK06688.
OMAiNDYPFKS.
PhylomeDBiQ9LJZ5.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciARA:AT3G20060-MONOMER.
ARA:GQT-2648-MONOMER.
ReactomeiREACT_273097. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_284122. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_294737. Senescence-Associated Secretory Phenotype (SASP).
REACT_295391. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_299093. Regulation of APC/C activators between G1/S and early anaphase.
REACT_301780. APC/C:Cdc20 mediated degradation of Securin.
REACT_305944. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_314276. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
REACT_318090. Separation of Sister Chromatids.
REACT_319658. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_336371. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_353749. APC-Cdc20 mediated degradation of Nek2A.

Gene expression databases

GenevestigatoriQ9LJZ5.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of plant ubiquitin-conjugating enzymes belonging to the UbcP4/E2-C/UBCx/UbcH10 gene family."
    Criqui M.C., de Almeida Engler J., Camasses A., Capron A., Parmentier Y., Inze D., Genschik P.
    Plant Physiol. 130:1230-1240(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION, MUTAGENESIS OF CYS-120.
  2. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, NOMENCLATURE.
  3. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Arabidopsis ORF clones."
    Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  6. "Arabidopsis anaphase-promoting complexes: multiple activators and wide range of substrates might keep APC perpetually busy."
    Fueloep K., Tarayre S., Kelemen Z., Horvath G., Kevei Z., Nikovics K., Bako L., Brown S., Kondorosi A., Kondorosi E.
    Cell Cycle 4:1084-1092(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiUBC19_ARATH
AccessioniPrimary (citable) accession number: Q9LJZ5
Secondary accession number(s): A8MSD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 2008
Last sequence update: September 30, 2000
Last modified: March 31, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.