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Q9LJZ5

- UBC19_ARATH

UniProt

Q9LJZ5 - UBC19_ARATH

Protein

Ubiquitin-conjugating enzyme E2 19

Gene

UBC19

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Part of the anaphase-promoting complex (APC). May have a key function during cell cycle and be involved in cyclin B1 degradation.1 Publication

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei120 – 1201Glycyl thioester intermediate

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: TAIR

    GO - Biological processi

    1. cell proliferation Source: TAIR
    2. ubiquitin-dependent protein catabolic process Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G20060-MONOMER.
    ARA:GQT-2648-MONOMER.
    ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_185297. Separation of Sister Chromatids.
    REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
    REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 19 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 19
    Gene namesi
    Name:UBC19
    Ordered Locus Names:At3g20060
    ORF Names:MAL21.6
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G20060.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: TAIR
    2. nucleus Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi120 – 1201C → A: Unable to form a complex with ubiquitin. 1 Publication
    Mutagenesisi120 – 1201C → S: Formation of a stable oxygen-ester bond with ubiquitin. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 181181Ubiquitin-conjugating enzyme E2 19PRO_0000345185Add
    BLAST

    Proteomic databases

    PaxDbiQ9LJZ5.

    Expressioni

    Tissue specificityi

    Expressed in all tissues with cell division activities and in mature leaves.1 Publication

    Developmental stagei

    Expressed during the G1-S phases of the cell cycle.1 Publication

    Inductioni

    Not induced by heat shock, dark to light transition, proteasome inhibitor MG132 or geldanamycin.1 Publication

    Gene expression databases

    GenevestigatoriQ9LJZ5.

    Interactioni

    Protein-protein interaction databases

    IntActiQ9LJZ5. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LJZ5.
    SMRiQ9LJZ5. Positions 37-176.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233454.
    InParanoidiQ9LJZ5.
    KOiK06688.
    PhylomeDBiQ9LJZ5.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9LJZ5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATVNGYTGN TPAATTPAAT GSKQSAPPTK TVDSHSVLKR LQSELMGLMM    50
    GADPGISAFP EEDNIFCWKG TITGSKDTVF EGTEYRLSLT FSNDYPFKSP 100
    KVKFETCCFH PNVDLYGNIC LDILQDKWSS AYDVRTILLS IQSLLGEPNI 150
    SSPLNNQAAQ LWSNQEEYRK MVEKLYKPLN A 181
    Length:181
    Mass (Da):19,999
    Last modified:October 1, 2000 - v1
    Checksum:iD4E5C632112D3796
    GO
    Isoform 2 (identifier: Q9LJZ5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-45: Missing.

    Note: Derived from EST data.

    Show »
    Length:136
    Mass (Da):15,431
    Checksum:iC24F807C1FED09F4
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 4545Missing in isoform 2. CuratedVSP_034927Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY127573 mRNA. Translation: AAM96886.1.
    DQ027033 mRNA. Translation: AAY44859.1.
    AP000383 Genomic DNA. Translation: BAB01863.1.
    CP002686 Genomic DNA. Translation: AEE76325.1.
    CP002686 Genomic DNA. Translation: AEE76326.1.
    BT025536 mRNA. Translation: ABF58954.1.
    RefSeqiNP_001078192.1. NM_001084723.1. [Q9LJZ5-2]
    NP_566653.1. NM_112897.3. [Q9LJZ5-1]
    UniGeneiAt.21408.

    Genome annotation databases

    EnsemblPlantsiAT3G20060.1; AT3G20060.1; AT3G20060. [Q9LJZ5-1]
    GeneIDi821545.
    KEGGiath:AT3G20060.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY127573 mRNA. Translation: AAM96886.1 .
    DQ027033 mRNA. Translation: AAY44859.1 .
    AP000383 Genomic DNA. Translation: BAB01863.1 .
    CP002686 Genomic DNA. Translation: AEE76325.1 .
    CP002686 Genomic DNA. Translation: AEE76326.1 .
    BT025536 mRNA. Translation: ABF58954.1 .
    RefSeqi NP_001078192.1. NM_001084723.1. [Q9LJZ5-2 ]
    NP_566653.1. NM_112897.3. [Q9LJZ5-1 ]
    UniGenei At.21408.

    3D structure databases

    ProteinModelPortali Q9LJZ5.
    SMRi Q9LJZ5. Positions 37-176.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9LJZ5. 1 interaction.

    Proteomic databases

    PaxDbi Q9LJZ5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G20060.1 ; AT3G20060.1 ; AT3G20060 . [Q9LJZ5-1 ]
    GeneIDi 821545.
    KEGGi ath:AT3G20060.

    Organism-specific databases

    GeneFarmi 2132. 224.
    TAIRi AT3G20060.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233454.
    InParanoidi Q9LJZ5.
    KOi K06688.
    PhylomeDBi Q9LJZ5.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT3G20060-MONOMER.
    ARA:GQT-2648-MONOMER.
    Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_185297. Separation of Sister Chromatids.
    REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
    REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

    Gene expression databases

    Genevestigatori Q9LJZ5.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of plant ubiquitin-conjugating enzymes belonging to the UbcP4/E2-C/UBCx/UbcH10 gene family."
      Criqui M.C., de Almeida Engler J., Camasses A., Capron A., Parmentier Y., Inze D., Genschik P.
      Plant Physiol. 130:1230-1240(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION, MUTAGENESIS OF CYS-120.
    2. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, NOMENCLATURE.
    3. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
      DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Arabidopsis ORF clones."
      Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.
      Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    6. "Arabidopsis anaphase-promoting complexes: multiple activators and wide range of substrates might keep APC perpetually busy."
      Fueloep K., Tarayre S., Kelemen Z., Horvath G., Kevei Z., Nikovics K., Bako L., Brown S., Kondorosi A., Kondorosi E.
      Cell Cycle 4:1084-1092(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiUBC19_ARATH
    AccessioniPrimary (citable) accession number: Q9LJZ5
    Secondary accession number(s): A8MSD2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3