Reviewed, UniProtKB/Swiss-Prot Q9LJZ5 (UBC19_ARATH)

Names and origin

Protein names

Recommended name:
    Ubiquitin carrier protein E2 19
    EC=6.3.2.19

Gene names

Name:	UBC19
Ordered Locus Names:	At3g20060
ORF Names:	MAL21.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Part of the anaphase-promoting complex (APC). May have a key function during cell cycle and be involved in cyclin B1 degradation. Ref.1
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Cytoplasm. Nucleus. Ref.1
Tissue specificity	Expressed in all tissues with cell division activities and in mature leaves. Ref.1
Developmental stage	Expressed during the G1-S phases of the cell cycle. Ref.5
Induction	Not induced by heat shock, dark to light transition, proteasome inhibitor MG132 or geldanamycin. Ref.1
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell proliferation Ref.1 Inferred from expression pattern. Source: TAIR protein ubiquitination Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro ubiquitin-dependent protein catabolic process Ref.2 Inferred from direct assay. Source: TAIR
Cellular component	cytoplasm Ref.1 Inferred from direct assay. Source: TAIR nucleus Ref.1 Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Ref.2 Inferred from direct assay. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9LJZ5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9LJZ5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.

Note: Derived from EST data.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	Ubiquitin carrier protein E2 19		PRO_0000345185
Sites
	Active site	120	1	Glycyl thioester intermediate
Natural variations
	Alternative sequence	1 – 45	45	Missing in isoform 2.		VSP_034927
Experimental info
	Mutagenesis	120	1	C → A: Unable to form a complex with ubiquitin. Ref.1
	Mutagenesis	120	1	C → S: Formation of a stable oxygen-ester bond with ubiquitin. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D4E5C632112D3796	FASTA	181	19,999
	10 20 30 40 50 60 MATVNGYTGN TPAATTPAAT GSKQSAPPTK TVDSHSVLKR LQSELMGLMM GADPGISAFP 70 80 90 100 110 120 EEDNIFCWKG TITGSKDTVF EGTEYRLSLT FSNDYPFKSP KVKFETCCFH PNVDLYGNIC 130 140 150 160 170 180 LDILQDKWSS AYDVRTILLS IQSLLGEPNI SSPLNNQAAQ LWSNQEEYRK MVEKLYKPLN A
	Isoform 2. Checksum: C24F807C1FED09F4	FASTA	136	15,431
	10 20 30 40 50 60 MGLMMGADPG ISAFPEEDNI FCWKGTITGS KDTVFEGTEY RLSLTFSNDY PFKSPKVKFE 70 80 90 100 110 120 TCCFHPNVDL YGNICLDILQ DKWSSAYDVR TILLSIQSLL GEPNISSPLN NQAAQLWSNQ 130 EEYRKMVEKL YKPLNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of plant ubiquitin-conjugating enzymes belonging to the UbcP4/E2-C/UBCx/UbcH10 gene family." Criqui M.C., de Almeida Engler J., Camasses A., Capron A., Parmentier Y., Inze D., Genschik P. Plant Physiol. 130:1230-1240(2002) [PubMed: 12427990] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION, MUTAGENESIS OF CYS-120.
[2]	"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis." Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J. Plant Physiol. 139:1597-1611(2005) [PubMed: 16339806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, NOMENCLATURE.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Arabidopsis anaphase-promoting complexes: multiple activators and wide range of substrates might keep APC perpetually busy." Fueloep K., Tarayre S., Kelemen Z., Horvath G., Kevei Z., Nikovics K., Bako L., Brown S., Kondorosi A., Kondorosi E. Cell Cycle 4:1084-1092(2005) [PubMed: 15970679] [Abstract] Cited for: DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY127573 mRNA. Translation: AAM96886.1. DQ027033 mRNA. Translation: AAY44859.1. AP000383 Genomic DNA. Translation: BAB01863.1. BT025536 mRNA. Translation: ABF58954.1.
IPI	IPI00519242. IPI00846253.
RefSeq	NP_001078192.1. NP_566653.1.
UniGene	At.21408
3D structure databases
HSSP	HSSP built from PDB template 1I7K based on UniProtKB O00762.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LJZ5. 1 interaction.
STRING	Q9LJZ5.
Genome annotation databases
GeneID	821545.
GenomeReviews	Gene locus AT3G20060 in contig BA000014_GR.
KEGG	ath:AT3G20060.
NMPDR	fig|3702.1.peg.14229.
Organism-specific databases
GeneFarm	2132. 224.
TAIR	At3g20060.
Phylogenomic databases
OMA	SSHKITI.
Gene expression databases
Genevestigator	Q9LJZ5.
Family and domain databases
InterPro	IPR015582. Ubiquitin-conj_enz_E2_H10. IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
PANTHER	PTHR11621:SF26. UbcH10. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC19_ARATH

Accession

Primary (citable) accession number: Q9LJZ5
Secondary accession number(s): A8MSD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)