Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9LJZ5 (UBC19_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 19

EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein 19
Gene names
Name:UBC19
Ordered Locus Names:At3g20060
ORF Names:MAL21.6
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length181 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Part of the anaphase-promoting complex (APC). May have a key function during cell cycle and be involved in cyclin B1 degradation. Ref.1

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subcellular location

Cytoplasm. Nucleus Ref.1.

Tissue specificity

Expressed in all tissues with cell division activities and in mature leaves. Ref.1

Developmental stage

Expressed during the G1-S phases of the cell cycle. Ref.6

Induction

Not induced by heat shock, dark to light transition, proteasome inhibitor MG132 or geldanamycin. Ref.1

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell proliferation

Inferred from expression pattern Ref.1. Source: TAIR

ubiquitin-dependent protein catabolic process

Inferred from direct assay Ref.2. Source: TAIR

   Cellular_componentcytoplasm

Inferred from direct assay Ref.1. Source: TAIR

nucleus

Inferred from direct assay Ref.1. Source: TAIR

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-protein ligase activity

Inferred from direct assay Ref.2. Source: TAIR

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9LJZ5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9LJZ5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.
Note: Derived from EST data.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 181181Ubiquitin-conjugating enzyme E2 19
PRO_0000345185

Sites

Active site1201Glycyl thioester intermediate

Natural variations

Alternative sequence1 – 4545Missing in isoform 2.
VSP_034927

Experimental info

Mutagenesis1201C → A: Unable to form a complex with ubiquitin. Ref.1
Mutagenesis1201C → S: Formation of a stable oxygen-ester bond with ubiquitin. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: D4E5C632112D3796

FASTA18119,999
        10         20         30         40         50         60 
MATVNGYTGN TPAATTPAAT GSKQSAPPTK TVDSHSVLKR LQSELMGLMM GADPGISAFP 

        70         80         90        100        110        120 
EEDNIFCWKG TITGSKDTVF EGTEYRLSLT FSNDYPFKSP KVKFETCCFH PNVDLYGNIC 

       130        140        150        160        170        180 
LDILQDKWSS AYDVRTILLS IQSLLGEPNI SSPLNNQAAQ LWSNQEEYRK MVEKLYKPLN 


A 

« Hide

Isoform 2 [UniParc].

Checksum: C24F807C1FED09F4
Show »

FASTA13615,431

References

« Hide 'large scale' references
[1]"Molecular characterization of plant ubiquitin-conjugating enzymes belonging to the UbcP4/E2-C/UBCx/UbcH10 gene family."
Criqui M.C., de Almeida Engler J., Camasses A., Capron A., Parmentier Y., Inze D., Genschik P.
Plant Physiol. 130:1230-1240(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION, MUTAGENESIS OF CYS-120.
[2]"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, NOMENCLATURE.
[3]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Arabidopsis ORF clones."
Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.
Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[6]"Arabidopsis anaphase-promoting complexes: multiple activators and wide range of substrates might keep APC perpetually busy."
Fueloep K., Tarayre S., Kelemen Z., Horvath G., Kevei Z., Nikovics K., Bako L., Brown S., Kondorosi A., Kondorosi E.
Cell Cycle 4:1084-1092(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY127573 mRNA. Translation: AAM96886.1.
DQ027033 mRNA. Translation: AAY44859.1.
AP000383 Genomic DNA. Translation: BAB01863.1.
CP002686 Genomic DNA. Translation: AEE76325.1.
CP002686 Genomic DNA. Translation: AEE76326.1.
BT025536 mRNA. Translation: ABF58954.1.
RefSeqNP_001078192.1. NM_001084723.1.
NP_566653.1. NM_112897.3.
UniGeneAt.21408.

3D structure databases

ProteinModelPortalQ9LJZ5.
SMRQ9LJZ5. Positions 37-176.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9LJZ5. 1 interaction.

Proteomic databases

PaxDbQ9LJZ5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G20060.1; AT3G20060.1; AT3G20060. [Q9LJZ5-1]
GeneID821545.
KEGGath:AT3G20060.

Organism-specific databases

GeneFarm2132. 224.
TAIRAT3G20060.

Phylogenomic databases

eggNOGCOG5078.
HOGENOMHOG000233454.
InParanoidQ9LJZ5.
KOK06688.
OMANDYPFKS.
PhylomeDBQ9LJZ5.
ProtClustDBCLSN2688586.

Enzyme and pathway databases

BioCycARA:AT3G20060-MONOMER.
ARA:GQT-2648-MONOMER.
UniPathwayUPA00143.

Gene expression databases

GenevestigatorQ9LJZ5.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBC19_ARATH
AccessionPrimary (citable) accession number: Q9LJZ5
Secondary accession number(s): A8MSD2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names