Reviewed,
UniProtKB/Swiss-Prot Q9LJZ5 (UBC19_ARATH)
Last modified
November 3, 2009.
Version 63.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Ubiquitin carrier protein E2 19 EC=6.3.2.19 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 181 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalyzes the covalent attachment of ubiquitin to other proteins. Part of the anaphase-promoting complex (APC). May have a key function during cell cycle and be involved in cyclin B1 degradation. Ref.1 |
| Catalytic activity | ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine. |
| Pathway | |
| Subcellular location | |
| Tissue specificity | Expressed in all tissues with cell division activities and in mature leaves. Ref.1 |
| Developmental stage | Expressed during the G1-S phases of the cell cycle. Ref.5 |
| Induction | Not induced by heat shock, dark to light transition, proteasome inhibitor MG132 or geldanamycin. Ref.1 |
| Sequence similarities | Belongs to the ubiquitin-conjugating enzyme family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Ubl conjugation pathway |
| Cellular component | Cytoplasm Nucleus |
| Coding sequence diversity | Alternative splicing |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | cell proliferation Ref.1 Inferred from expression pattern. Source: TAIR protein ubiquitinationInferred from electronic annotation. Source: InterPro regulation of protein metabolic processInferred from electronic annotation. Source: InterPro ubiquitin-dependent protein catabolic process Ref.2Inferred from direct assay. Source: TAIR |
| Cellular component | cytoplasm Ref.1 Inferred from direct assay. Source: TAIR nucleus Ref.1Inferred from direct assay. Source: TAIR |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Ref.2Inferred from direct assay. Source: TAIR |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q9LJZ5-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q9LJZ5-2) The sequence of this isoform differs from the canonical sequence as follows: 1-45: Missing. | ||||||
| Note: Derived from EST data. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 181 | 181 | Ubiquitin carrier protein E2 19 | PRO_0000345185 | |||||
Sites | |||||||||
| Active site | 120 | 1 | Glycyl thioester intermediate | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 45 | 45 | Missing in isoform 2. | VSP_034927 | |||||
Experimental info | |||||||||
| Mutagenesis | 120 | 1 | C → A: Unable to form a complex with ubiquitin. Ref.1 | ||||||
| Mutagenesis | 120 | 1 | C → S: Formation of a stable oxygen-ester bond with ubiquitin. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular characterization of plant ubiquitin-conjugating enzymes belonging to the UbcP4/E2-C/UBCx/UbcH10 gene family." Criqui M.C., de Almeida Engler J., Camasses A., Capron A., Parmentier Y., Inze D., Genschik P. Plant Physiol. 130:1230-1240(2002) [PubMed: 12427990] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION, MUTAGENESIS OF CYS-120. |
| [2] | "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis." Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J. Plant Physiol. 139:1597-1611(2005) [PubMed: 16339806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, NOMENCLATURE. |
| [3] | "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [4] | "Arabidopsis ORF clones." Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia. |
| [5] | "Arabidopsis anaphase-promoting complexes: multiple activators and wide range of substrates might keep APC perpetually busy." Fueloep K., Tarayre S., Kelemen Z., Horvath G., Kevei Z., Nikovics K., Bako L., Brown S., Kondorosi A., Kondorosi E. Cell Cycle 4:1084-1092(2005) [PubMed: 15970679] [Abstract] Cited for: DEVELOPMENTAL STAGE. |
| + | Additional computationally mapped references. |
Web resources
| PlantsUBQ A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants |
Cross-references
Sequence databases | |
|---|---|
| AY127573 mRNA. Translation: AAM96886.1. DQ027033 mRNA. Translation: AAY44859.1. AP000383 Genomic DNA. Translation: BAB01863.1. BT025536 mRNA. Translation: ABF58954.1. | |
| IPI | IPI00519242. IPI00846253. |
| RefSeq | NP_001078192.1. NP_566653.1. |
| UniGene | At.21408 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1I7K based on UniProtKB O00762. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9LJZ5. 1 interaction. |
| STRING | Q9LJZ5. |
Genome annotation databases | |
| GeneID | 821545. |
| GenomeReviews | Gene locus AT3G20060 in contig BA000014_GR. |
| KEGG | ath:AT3G20060. |
| NMPDR | fig|3702.1.peg.14229. |
Organism-specific databases | |
| GeneFarm | 2132. 224. |
| TAIR | At3g20060. |
Phylogenomic databases | |
| OMA | SSHKITI. |
Gene expression databases | |
| Genevestigator | Q9LJZ5. |
Family and domain databases | |
| InterPro | IPR015582. Ubiquitin-conj_enz_E2_H10. IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view] |
| Gene3D | G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit. |
| PANTHER | PTHR11621:SF26. UbcH10. 1 hit. |
| Pfam | PF00179. UQ_con. 1 hit. [Graphical view] |
| ProDom | PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00212. UBCc. 1 hit. [Graphical view] |
| PROSITE | PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | UBC19_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q9LJZ5 Secondary accession number(s): A8MSD2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


