Q9LJZ5 (UBC19_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 19
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein 19

Gene names

Name:	UBC19
Ordered Locus Names:	At3g20060
ORF Names:	MAL21.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Part of the anaphase-promoting complex (APC). May have a key function during cell cycle and be involved in cyclin B1 degradation. Ref.1
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Cytoplasm. Nucleus Ref.1.
Tissue specificity	Expressed in all tissues with cell division activities and in mature leaves. Ref.1
Developmental stage	Expressed during the G1-S phases of the cell cycle. Ref.6
Induction	Not induced by heat shock, dark to light transition, proteasome inhibitor MG132 or geldanamycin. Ref.1
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell proliferation Inferred from expression pattern Ref.1. Source: TAIR ubiquitin-dependent protein catabolic process Inferred from direct assay Ref.2. Source: TAIR
Cellular_component	cytoplasm Inferred from direct assay Ref.1. Source: TAIR nucleus Inferred from direct assay Ref.1. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from direct assay Ref.2. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9LJZ5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9LJZ5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.

Note: Derived from EST data.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	Ubiquitin-conjugating enzyme E2 19		PRO_0000345185
Sites
	Active site	120	1	Glycyl thioester intermediate
Natural variations
	Alternative sequence	1 – 45	45	Missing in isoform 2.		VSP_034927
Experimental info
	Mutagenesis	120	1	C → A: Unable to form a complex with ubiquitin. Ref.1
	Mutagenesis	120	1	C → S: Formation of a stable oxygen-ester bond with ubiquitin. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D4E5C632112D3796	FASTA	181	19,999
	10 20 30 40 50 60 MATVNGYTGN TPAATTPAAT GSKQSAPPTK TVDSHSVLKR LQSELMGLMM GADPGISAFP 70 80 90 100 110 120 EEDNIFCWKG TITGSKDTVF EGTEYRLSLT FSNDYPFKSP KVKFETCCFH PNVDLYGNIC 130 140 150 160 170 180 LDILQDKWSS AYDVRTILLS IQSLLGEPNI SSPLNNQAAQ LWSNQEEYRK MVEKLYKPLN A
	Isoform 2 [UniParc]. Checksum: C24F807C1FED09F4	FASTA	136	15,431
	10 20 30 40 50 60 MGLMMGADPG ISAFPEEDNI FCWKGTITGS KDTVFEGTEY RLSLTFSNDY PFKSPKVKFE 70 80 90 100 110 120 TCCFHPNVDL YGNICLDILQ DKWSSAYDVR TILLSIQSLL GEPNISSPLN NQAAQLWSNQ 130 EEYRKMVEKL YKPLNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of plant ubiquitin-conjugating enzymes belonging to the UbcP4/E2-C/UBCx/UbcH10 gene family." Criqui M.C., de Almeida Engler J., Camasses A., Capron A., Parmentier Y., Inze D., Genschik P. Plant Physiol. 130:1230-1240(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION, MUTAGENESIS OF CYS-120.
[2]	"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis." Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J. Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, NOMENCLATURE.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Arabidopsis ORF clones." Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[6]	"Arabidopsis anaphase-promoting complexes: multiple activators and wide range of substrates might keep APC perpetually busy." Fueloep K., Tarayre S., Kelemen Z., Horvath G., Kevei Z., Nikovics K., Bako L., Brown S., Kondorosi A., Kondorosi E. Cell Cycle 4:1084-1092(2005) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY127573 mRNA. Translation: AAM96886.1. DQ027033 mRNA. Translation: AAY44859.1. AP000383 Genomic DNA. Translation: BAB01863.1. CP002686 Genomic DNA. Translation: AEE76325.1. CP002686 Genomic DNA. Translation: AEE76326.1. BT025536 mRNA. Translation: ABF58954.1.
IPI	IPI00519242. IPI00846253.
RefSeq	NP_001078192.1. NM_001084723.1. NP_566653.1. NM_112897.3.
UniGene	At.21408.
3D structure databases
HSSP	HSSP built from PDB template 1I7K based on UniProtKB O00762.
ProteinModelPortal	Q9LJZ5.
SMR	Q9LJZ5. Positions 37-176.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LJZ5. 1 interaction.
Proteomic databases
PaxDb	Q9LJZ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G20060.1; AT3G20060.1; AT3G20060.
GeneID	821545.
KEGG	ath:AT3G20060.
Organism-specific databases
GeneFarm	2132. 224.
TAIR	At3g20060.
Phylogenomic databases
eggNOG	COG5078.
HOGENOM	HOG000233454.
InParanoid	Q9LJZ5.
KO	K06688.
OMA	MATVNGY.
PhylomeDB	Q9LJZ5.
ProtClustDB	CLSN2688586.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	Q9LJZ5.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC19_ARATH

Accession

Primary (citable) accession number: Q9LJZ5
Secondary accession number(s): A8MSD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program