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Q9LJU7

- PPA18_ARATH

UniProt

Q9LJU7 - PPA18_ARATH

Protein

Purple acid phosphatase 18

Gene

PAP18

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.

    Cofactori

    Binds 1 iron ion per subunit.By similarity
    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi148 – 1481IronBy similarity
    Metal bindingi175 – 1751IronBy similarity
    Metal bindingi175 – 1751ZincBy similarity
    Metal bindingi178 – 1781IronBy similarity
    Metal bindingi208 – 2081ZincBy similarity
    Binding sitei208 – 2081SubstrateBy similarity
    Metal bindingi291 – 2911ZincBy similarity
    Active sitei301 – 3011Proton donorBy similarity
    Metal bindingi328 – 3281ZincBy similarity
    Metal bindingi330 – 3301IronBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: TAIR
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Iron, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT3G20500-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Purple acid phosphatase 18 (EC:3.1.3.2)
    Gene namesi
    Name:PAP18
    Synonyms:PAP30
    Ordered Locus Names:At3g20500
    ORF Names:K10D20.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G20500.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 437414Purple acid phosphatase 18PRO_0000372821Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi390 – 3901N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9LJU7.
    PRIDEiQ9LJU7.

    Expressioni

    Tissue specificityi

    Expressed in roots, stems, leaves, flowers and siliques.1 Publication

    Gene expression databases

    GenevestigatoriQ9LJU7.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LJU7.
    SMRiQ9LJU7. Positions 45-414.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni328 – 3303Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1409.
    HOGENOMiHOG000238330.
    InParanoidiQ9LJU7.
    OMAiHEKESIP.
    PhylomeDBiQ9LJU7.

    Family and domain databases

    Gene3Di2.60.40.380. 1 hit.
    3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9LJU7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKWGILLLV TLSVSIIFTS AAADDYVRPK PRETLQFPWK QKSSSVPEQV    50
    HISLAGDKHM RVTWVTNDKS SPSFVEYGTS PGKYSYLGQG ESTSYSYIMY 100
    RSGKIHHTVI GPLEADTVYY YRCGGEGPEF HLKTPPAQFP ITFAVAGDLG 150
    QTGWTKSTLD HIDQCKYAVH LLPGDLSYAD YMQHKWDTFG ELVQPLASVR 200
    PWMVTQGNHE KESIPFIVDE FVSFNSRWKM PYEESGSNSN LYYSFEVAGV 250
    HAIMLGSYTD YDRYSDQYSW LKADLSKVDR ERTPWLIVLF HVPWYNSNNA 300
    HQHEGDEMMA EMEPLLYASG VDIVFTGHVH AYERTKRVNN GKSDPCGPVH 350
    ITIGDGGNRE GLARKYKDPS PEWSVFREAS FGHGELQMVN STHALWTWHR 400
    NDDDEPTRSD EVWLNSLVNS GCLKKRPQEL RKMLLEP 437
    Length:437
    Mass (Da):49,870
    Last modified:October 1, 2000 - v1
    Checksum:i1CD48C6F4E8E3ABE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti212 – 2121E → A in AAP81215. 1 PublicationCurated
    Sequence conflicti242 – 2421Y → N in AAN74649. (PubMed:16244908)Curated
    Sequence conflicti395 – 3951L → I in AAN74649. (PubMed:16244908)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF448725 mRNA. Translation: AAN74649.1.
    AP000410 Genomic DNA. Translation: BAB01159.1.
    CP002686 Genomic DNA. Translation: AEE76388.1.
    AY062488 mRNA. Translation: AAL32566.1.
    AY093272 mRNA. Translation: AAM13271.1.
    AY297742 mRNA. Translation: AAP81215.1.
    RefSeqiNP_188686.2. NM_112942.4.
    UniGeneiAt.24149.

    Genome annotation databases

    EnsemblPlantsiAT3G20500.1; AT3G20500.1; AT3G20500.
    GeneIDi821596.
    KEGGiath:AT3G20500.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF448725 mRNA. Translation: AAN74649.1 .
    AP000410 Genomic DNA. Translation: BAB01159.1 .
    CP002686 Genomic DNA. Translation: AEE76388.1 .
    AY062488 mRNA. Translation: AAL32566.1 .
    AY093272 mRNA. Translation: AAM13271.1 .
    AY297742 mRNA. Translation: AAP81215.1 .
    RefSeqi NP_188686.2. NM_112942.4.
    UniGenei At.24149.

    3D structure databases

    ProteinModelPortali Q9LJU7.
    SMRi Q9LJU7. Positions 45-414.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q9LJU7.
    PRIDEi Q9LJU7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G20500.1 ; AT3G20500.1 ; AT3G20500 .
    GeneIDi 821596.
    KEGGi ath:AT3G20500.

    Organism-specific databases

    TAIRi AT3G20500.

    Phylogenomic databases

    eggNOGi COG1409.
    HOGENOMi HOG000238330.
    InParanoidi Q9LJU7.
    OMAi HEKESIP.
    PhylomeDBi Q9LJU7.

    Enzyme and pathway databases

    BioCyci ARA:AT3G20500-MONOMER.

    Gene expression databases

    Genevestigatori Q9LJU7.

    Family and domain databases

    Gene3Di 2.60.40.380. 1 hit.
    3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
      Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
      Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
      DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Identification of differentially displayed Arabidopsis thaliana acid phosphatase-encoding genes."
      Lohrasebi T., Malboobi M.A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 212-332.
      Tissue: Seedling.
    6. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
      Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
      J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPPA18_ARATH
    AccessioniPrimary (citable) accession number: Q9LJU7
    Secondary accession number(s): Q7XY13, Q8H6W7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3