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Protein

Purple acid phosphatase 18

Gene

PAP18

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi148IronBy similarity1
Metal bindingi175IronBy similarity1
Metal bindingi175ZincBy similarity1
Metal bindingi178IronBy similarity1
Metal bindingi208ZincBy similarity1
Binding sitei208SubstrateBy similarity1
Metal bindingi291ZincBy similarity1
Active sitei301Proton donorBy similarity1
Metal bindingi328ZincBy similarity1
Metal bindingi330IronBy similarity1

GO - Molecular functioni

  • acid phosphatase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase
LigandIron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT3G20500-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 18 (EC:3.1.3.2)
Gene namesi
Name:PAP18
Synonyms:PAP30
Ordered Locus Names:At3g20500
ORF Names:K10D20.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G20500
TAIRilocus:2085770 AT3G20500

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000037282124 – 437Purple acid phosphatase 18Add BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi390N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9LJU7

PTM databases

iPTMnetiQ9LJU7

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ9LJU7 baseline and differential
GenevisibleiQ9LJU7 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G20500.1

Structurei

3D structure databases

ProteinModelPortaliQ9LJU7
SMRiQ9LJU7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni328 – 330Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1378 Eukaryota
COG1409 LUCA
HOGENOMiHOG000238330
InParanoidiQ9LJU7
KOiK22390
OMAiGHVHNSQ
OrthoDBiEOG09360BFU
PhylomeDBiQ9LJU7

Family and domain databases

Gene3Di2.60.40.3801 hit
3.60.21.101 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR008963 Purple_acid_Pase-like_N
IPR015914 Purple_acid_Pase_N
IPR025733 Purple_acid_PPase_C_dom
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PF14008 Metallophos_C, 1 hit
PF16656 Pur_ac_phosph_N, 1 hit
SUPFAMiSSF49363 SSF49363, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LJU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKWGILLLV TLSVSIIFTS AAADDYVRPK PRETLQFPWK QKSSSVPEQV
60 70 80 90 100
HISLAGDKHM RVTWVTNDKS SPSFVEYGTS PGKYSYLGQG ESTSYSYIMY
110 120 130 140 150
RSGKIHHTVI GPLEADTVYY YRCGGEGPEF HLKTPPAQFP ITFAVAGDLG
160 170 180 190 200
QTGWTKSTLD HIDQCKYAVH LLPGDLSYAD YMQHKWDTFG ELVQPLASVR
210 220 230 240 250
PWMVTQGNHE KESIPFIVDE FVSFNSRWKM PYEESGSNSN LYYSFEVAGV
260 270 280 290 300
HAIMLGSYTD YDRYSDQYSW LKADLSKVDR ERTPWLIVLF HVPWYNSNNA
310 320 330 340 350
HQHEGDEMMA EMEPLLYASG VDIVFTGHVH AYERTKRVNN GKSDPCGPVH
360 370 380 390 400
ITIGDGGNRE GLARKYKDPS PEWSVFREAS FGHGELQMVN STHALWTWHR
410 420 430
NDDDEPTRSD EVWLNSLVNS GCLKKRPQEL RKMLLEP
Length:437
Mass (Da):49,870
Last modified:October 1, 2000 - v1
Checksum:i1CD48C6F4E8E3ABE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti212E → A in AAP81215 (Ref. 5) Curated1
Sequence conflicti242Y → N in AAN74649 (PubMed:16244908).Curated1
Sequence conflicti395L → I in AAN74649 (PubMed:16244908).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448725 mRNA Translation: AAN74649.1
AP000410 Genomic DNA Translation: BAB01159.1
CP002686 Genomic DNA Translation: AEE76388.1
AY062488 mRNA Translation: AAL32566.1
AY093272 mRNA Translation: AAM13271.1
AY297742 mRNA Translation: AAP81215.1
RefSeqiNP_188686.2, NM_112942.5
UniGeneiAt.24149

Genome annotation databases

EnsemblPlantsiAT3G20500.1; AT3G20500.1; AT3G20500
GeneIDi821596
GrameneiAT3G20500.1; AT3G20500.1; AT3G20500
KEGGiath:AT3G20500

Similar proteinsi

Entry informationi

Entry nameiPPA18_ARATH
AccessioniPrimary (citable) accession number: Q9LJU7
Secondary accession number(s): Q7XY13, Q8H6W7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome