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Q9LJU7

- PPA18_ARATH

UniProt

Q9LJU7 - PPA18_ARATH

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Protein

Purple acid phosphatase 18

Gene

PAP18

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Binds 1 iron ion per subunit.By similarity
Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi148 – 1481IronBy similarity
Metal bindingi175 – 1751IronBy similarity
Metal bindingi175 – 1751ZincBy similarity
Metal bindingi178 – 1781IronBy similarity
Metal bindingi208 – 2081ZincBy similarity
Binding sitei208 – 2081SubstrateBy similarity
Metal bindingi291 – 2911ZincBy similarity
Active sitei301 – 3011Proton donorBy similarity
Metal bindingi328 – 3281ZincBy similarity
Metal bindingi330 – 3301IronBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: TAIR
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT3G20500-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 18 (EC:3.1.3.2)
Gene namesi
Name:PAP18
Synonyms:PAP30
Ordered Locus Names:At3g20500
ORF Names:K10D20.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G20500.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 437414Purple acid phosphatase 18PRO_0000372821Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi390 – 3901N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9LJU7.
PRIDEiQ9LJU7.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Gene expression databases

GenevestigatoriQ9LJU7.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9LJU7.
SMRiQ9LJU7. Positions 45-414.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni328 – 3303Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1409.
HOGENOMiHOG000238330.
InParanoidiQ9LJU7.
OMAiHEKESIP.
PhylomeDBiQ9LJU7.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LJU7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEKWGILLLV TLSVSIIFTS AAADDYVRPK PRETLQFPWK QKSSSVPEQV
60 70 80 90 100
HISLAGDKHM RVTWVTNDKS SPSFVEYGTS PGKYSYLGQG ESTSYSYIMY
110 120 130 140 150
RSGKIHHTVI GPLEADTVYY YRCGGEGPEF HLKTPPAQFP ITFAVAGDLG
160 170 180 190 200
QTGWTKSTLD HIDQCKYAVH LLPGDLSYAD YMQHKWDTFG ELVQPLASVR
210 220 230 240 250
PWMVTQGNHE KESIPFIVDE FVSFNSRWKM PYEESGSNSN LYYSFEVAGV
260 270 280 290 300
HAIMLGSYTD YDRYSDQYSW LKADLSKVDR ERTPWLIVLF HVPWYNSNNA
310 320 330 340 350
HQHEGDEMMA EMEPLLYASG VDIVFTGHVH AYERTKRVNN GKSDPCGPVH
360 370 380 390 400
ITIGDGGNRE GLARKYKDPS PEWSVFREAS FGHGELQMVN STHALWTWHR
410 420 430
NDDDEPTRSD EVWLNSLVNS GCLKKRPQEL RKMLLEP
Length:437
Mass (Da):49,870
Last modified:October 1, 2000 - v1
Checksum:i1CD48C6F4E8E3ABE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti212 – 2121E → A in AAP81215. 1 PublicationCurated
Sequence conflicti242 – 2421Y → N in AAN74649. (PubMed:16244908)Curated
Sequence conflicti395 – 3951L → I in AAN74649. (PubMed:16244908)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF448725 mRNA. Translation: AAN74649.1.
AP000410 Genomic DNA. Translation: BAB01159.1.
CP002686 Genomic DNA. Translation: AEE76388.1.
AY062488 mRNA. Translation: AAL32566.1.
AY093272 mRNA. Translation: AAM13271.1.
AY297742 mRNA. Translation: AAP81215.1.
RefSeqiNP_188686.2. NM_112942.4.
UniGeneiAt.24149.

Genome annotation databases

EnsemblPlantsiAT3G20500.1; AT3G20500.1; AT3G20500.
GeneIDi821596.
KEGGiath:AT3G20500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF448725 mRNA. Translation: AAN74649.1 .
AP000410 Genomic DNA. Translation: BAB01159.1 .
CP002686 Genomic DNA. Translation: AEE76388.1 .
AY062488 mRNA. Translation: AAL32566.1 .
AY093272 mRNA. Translation: AAM13271.1 .
AY297742 mRNA. Translation: AAP81215.1 .
RefSeqi NP_188686.2. NM_112942.4.
UniGenei At.24149.

3D structure databases

ProteinModelPortali Q9LJU7.
SMRi Q9LJU7. Positions 45-414.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q9LJU7.
PRIDEi Q9LJU7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G20500.1 ; AT3G20500.1 ; AT3G20500 .
GeneIDi 821596.
KEGGi ath:AT3G20500.

Organism-specific databases

TAIRi AT3G20500.

Phylogenomic databases

eggNOGi COG1409.
HOGENOMi HOG000238330.
InParanoidi Q9LJU7.
OMAi HEKESIP.
PhylomeDBi Q9LJU7.

Enzyme and pathway databases

BioCyci ARA:AT3G20500-MONOMER.

Gene expression databases

Genevestigatori Q9LJU7.

Family and domain databases

Gene3Di 2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view ]
SUPFAMi SSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
    Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
    Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Identification of differentially displayed Arabidopsis thaliana acid phosphatase-encoding genes."
    Lohrasebi T., Malboobi M.A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 212-332.
    Tissue: Seedling.
  6. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
    Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
    J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPPA18_ARATH
AccessioniPrimary (citable) accession number: Q9LJU7
Secondary accession number(s): Q7XY13, Q8H6W7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3