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Q9LJU7 (PPA18_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Purple acid phosphatase 18

EC=3.1.3.2
Gene names
Name:PAP18
Synonyms:PAP30
Ordered Locus Names:At3g20500
ORF Names:K10D20.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length437 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Secreted By similarity.

Tissue specificity

Expressed in roots, stems, leaves, flowers and siliques. Ref.1

Sequence similarities

Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandIron
Metal-binding
Zinc
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdephosphorylation

Inferred from sequence or structural similarity Ref.1. Source: GOC

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionacid phosphatase activity

Inferred from sequence or structural similarity Ref.1. Source: TAIR

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 437414Purple acid phosphatase 18
PRO_0000372821

Regions

Region328 – 3303Substrate binding By similarity

Sites

Active site3011Proton donor By similarity
Metal binding1481Iron By similarity
Metal binding1751Iron By similarity
Metal binding1751Zinc By similarity
Metal binding1781Iron By similarity
Metal binding2081Zinc By similarity
Metal binding2911Zinc By similarity
Metal binding3281Zinc By similarity
Metal binding3301Iron By similarity
Binding site2081Substrate By similarity

Amino acid modifications

Glycosylation3901N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict2121E → A in AAP81215. Ref.5
Sequence conflict2421Y → N in AAN74649. Ref.1
Sequence conflict3951L → I in AAN74649. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9LJU7 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 1CD48C6F4E8E3ABE

FASTA43749,870
        10         20         30         40         50         60 
MEKWGILLLV TLSVSIIFTS AAADDYVRPK PRETLQFPWK QKSSSVPEQV HISLAGDKHM 

        70         80         90        100        110        120 
RVTWVTNDKS SPSFVEYGTS PGKYSYLGQG ESTSYSYIMY RSGKIHHTVI GPLEADTVYY 

       130        140        150        160        170        180 
YRCGGEGPEF HLKTPPAQFP ITFAVAGDLG QTGWTKSTLD HIDQCKYAVH LLPGDLSYAD 

       190        200        210        220        230        240 
YMQHKWDTFG ELVQPLASVR PWMVTQGNHE KESIPFIVDE FVSFNSRWKM PYEESGSNSN 

       250        260        270        280        290        300 
LYYSFEVAGV HAIMLGSYTD YDRYSDQYSW LKADLSKVDR ERTPWLIVLF HVPWYNSNNA 

       310        320        330        340        350        360 
HQHEGDEMMA EMEPLLYASG VDIVFTGHVH AYERTKRVNN GKSDPCGPVH ITIGDGGNRE 

       370        380        390        400        410        420 
GLARKYKDPS PEWSVFREAS FGHGELQMVN STHALWTWHR NDDDEPTRSD EVWLNSLVNS 

       430 
GCLKKRPQEL RKMLLEP 

« Hide

References

« Hide 'large scale' references
[1]"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Identification of differentially displayed Arabidopsis thaliana acid phosphatase-encoding genes."
Lohrasebi T., Malboobi M.A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 212-332.
Tissue: Seedling.
[6]"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF448725 mRNA. Translation: AAN74649.1.
AP000410 Genomic DNA. Translation: BAB01159.1.
CP002686 Genomic DNA. Translation: AEE76388.1.
AY062488 mRNA. Translation: AAL32566.1.
AY093272 mRNA. Translation: AAM13271.1.
AY297742 mRNA. Translation: AAP81215.1.
RefSeqNP_188686.2. NM_112942.4.
UniGeneAt.24149.

3D structure databases

ProteinModelPortalQ9LJU7.
SMRQ9LJU7. Positions 45-414.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ9LJU7.
PRIDEQ9LJU7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G20500.1; AT3G20500.1; AT3G20500.
GeneID821596.
KEGGath:AT3G20500.

Organism-specific databases

TAIRAT3G20500.

Phylogenomic databases

eggNOGCOG1409.
HOGENOMHOG000238330.
InParanoidQ9LJU7.
OMAHEKESIP.
PhylomeDBQ9LJU7.

Enzyme and pathway databases

BioCycARA:AT3G20500-MONOMER.

Gene expression databases

GenevestigatorQ9LJU7.

Family and domain databases

Gene3D2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SUPFAMSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.
ProtoNetSearch...

Entry information

Entry namePPA18_ARATH
AccessionPrimary (citable) accession number: Q9LJU7
Secondary accession number(s): Q7XY13, Q8H6W7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names