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Q9LJR7

- XTH3_ARATH

UniProt

Q9LJR7 - XTH3_ARATH

Protein

Xyloglucan endotransglucosylase/hydrolase protein 3

Gene

XTH3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.By similarity

    Catalytic activityi

    Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei109 – 1091NucleophileBy similarity
    Active sitei113 – 1131Proton donorBy similarity

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
    2. xyloglucan:xyloglucosyl transferase activity Source: TAIR

    GO - Biological processi

    1. cellular glucan metabolic process Source: InterPro
    2. photoperiodism, flowering Source: TAIR
    3. plant-type cell wall biogenesis Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciARA:AT3G25050-MONOMER.

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xyloglucan endotransglucosylase/hydrolase protein 3 (EC:2.4.1.207)
    Short name:
    At-XTH3
    Short name:
    XTH-3
    Gene namesi
    Name:XTH3
    Ordered Locus Names:At3g25050
    ORF Names:K3G3.6
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G25050.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 290269Xyloglucan endotransglucosylase/hydrolase protein 3PRO_0000011803Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi210 – 2101N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ9LJR7.
    PRIDEiQ9LJR7.

    Expressioni

    Tissue specificityi

    Predominantly expressed in flower buds.1 Publication

    Inductioni

    By auxin and brassinolide.1 Publication

    Gene expression databases

    GenevestigatoriQ9LJR7.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LJR7.
    SMRiQ9LJR7. Positions 35-289.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2273.
    InParanoidiQ9LJR7.
    KOiK08235.
    OMAiGACESSN.
    PhylomeDBiQ9LJR7.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008264. Beta_glucanase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005604. XET. 1 hit.
    PRINTSiPR00737. GLHYDRLASE16.
    SUPFAMiSSF49899. SSF49899. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9LJR7-1 [UniParc]FASTAAdd to Basket

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    MDYMRIFSVF VVTLWIIRVD ARVFGGRGIE KFVTFGQNYI VTWGQSHVST    50
    LHSGEEVDLY MDQSSGGGFE SKDAYGSGLF EMRIKVPSGN TGGIVTAFYL 100
    TSKGGGHDEI DFEFLGNNNG KPVTLQTNLF LNGEGNREER FLLWFNPTKH 150
    YHTYGLLWNP YQIVFYVDNI PIRVYKNENG VSYPSKPMQV EASLWNGDDW 200
    ATDGGRTKVN WSYSPFIAHF RDFALSGCNI DGRSNNVGAC ESSNYWWNAG 250
    NYQRLSGNEQ KLYEHVRSKY MNYDYCTDRS KYQTPPRECY 290
    Length:290
    Mass (Da):33,259
    Last modified:October 1, 2000 - v1
    Checksum:i5139E94737955AF0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP000412 Genomic DNA. Translation: BAB01890.1.
    CP002686 Genomic DNA. Translation: AEE76972.1.
    DQ446697 mRNA. Translation: ABE65966.1.
    RefSeqiNP_189141.1. NM_113409.4.
    UniGeneiAt.46272.

    Genome annotation databases

    EnsemblPlantsiAT3G25050.1; AT3G25050.1; AT3G25050.
    GeneIDi822096.
    KEGGiath:AT3G25050.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP000412 Genomic DNA. Translation: BAB01890.1 .
    CP002686 Genomic DNA. Translation: AEE76972.1 .
    DQ446697 mRNA. Translation: ABE65966.1 .
    RefSeqi NP_189141.1. NM_113409.4.
    UniGenei At.46272.

    3D structure databases

    ProteinModelPortali Q9LJR7.
    SMRi Q9LJR7. Positions 35-289.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Proteomic databases

    PaxDbi Q9LJR7.
    PRIDEi Q9LJR7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G25050.1 ; AT3G25050.1 ; AT3G25050 .
    GeneIDi 822096.
    KEGGi ath:AT3G25050.

    Organism-specific databases

    TAIRi AT3G25050.

    Phylogenomic databases

    eggNOGi COG2273.
    InParanoidi Q9LJR7.
    KOi K08235.
    OMAi GACESSN.
    PhylomeDBi Q9LJR7.

    Enzyme and pathway databases

    BioCyci ARA:AT3G25050-MONOMER.

    Gene expression databases

    Genevestigatori Q9LJR7.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008264. Beta_glucanase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005604. XET. 1 hit.
    PRINTSi PR00737. GLHYDRLASE16.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
      DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations."
      Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.
      Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
      Yokoyama R., Nishitani K.
      Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.
    5. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
      Rose J.K.C., Braam J., Fry S.C., Nishitani K.
      Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE.

    Entry informationi

    Entry nameiXTH3_ARATH
    AccessioniPrimary (citable) accession number: Q9LJR7
    Secondary accession number(s): Q1PEM0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2003
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3