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Q9LJR7

- XTH3_ARATH

UniProt

Q9LJR7 - XTH3_ARATH

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Protein

Xyloglucan endotransglucosylase/hydrolase protein 3

Gene

XTH3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues (By similarity).By similarity

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei109 – 1091NucleophileBy similarity
Active sitei113 – 1131Proton donorBy similarity

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  2. xyloglucan:xyloglucosyl transferase activity Source: TAIR

GO - Biological processi

  1. cellular glucan metabolic process Source: InterPro
  2. cell wall organization Source: UniProtKB-KW
  3. photoperiodism, flowering Source: TAIR
  4. plant-type cell wall biogenesis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT3G25050-MONOMER.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Xyloglucan endotransglucosylase/hydrolase protein 3 (EC:2.4.1.207)
Short name:
At-XTH3
Short name:
XTH-3
Gene namesi
Name:XTH3
Ordered Locus Names:At3g25050
ORF Names:K3G3.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G25050.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-KW
  2. cell wall Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 290269Xyloglucan endotransglucosylase/hydrolase protein 3PRO_0000011803Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi210 – 2101N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9LJR7.
PRIDEiQ9LJR7.

Expressioni

Tissue specificityi

Predominantly expressed in flower buds.1 Publication

Inductioni

By auxin and brassinolide.1 Publication

Gene expression databases

ExpressionAtlasiQ9LJR7. differential.
GenevestigatoriQ9LJR7.

Structurei

3D structure databases

ProteinModelPortaliQ9LJR7.
SMRiQ9LJR7. Positions 35-289.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2273.
InParanoidiQ9LJR7.
KOiK08235.
OMAiGACESSN.
PhylomeDBiQ9LJR7.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LJR7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYMRIFSVF VVTLWIIRVD ARVFGGRGIE KFVTFGQNYI VTWGQSHVST
60 70 80 90 100
LHSGEEVDLY MDQSSGGGFE SKDAYGSGLF EMRIKVPSGN TGGIVTAFYL
110 120 130 140 150
TSKGGGHDEI DFEFLGNNNG KPVTLQTNLF LNGEGNREER FLLWFNPTKH
160 170 180 190 200
YHTYGLLWNP YQIVFYVDNI PIRVYKNENG VSYPSKPMQV EASLWNGDDW
210 220 230 240 250
ATDGGRTKVN WSYSPFIAHF RDFALSGCNI DGRSNNVGAC ESSNYWWNAG
260 270 280 290
NYQRLSGNEQ KLYEHVRSKY MNYDYCTDRS KYQTPPRECY
Length:290
Mass (Da):33,259
Last modified:October 1, 2000 - v1
Checksum:i5139E94737955AF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000412 Genomic DNA. Translation: BAB01890.1.
CP002686 Genomic DNA. Translation: AEE76972.1.
DQ446697 mRNA. Translation: ABE65966.1.
RefSeqiNP_189141.1. NM_113409.4.
UniGeneiAt.46272.

Genome annotation databases

EnsemblPlantsiAT3G25050.1; AT3G25050.1; AT3G25050.
GeneIDi822096.
KEGGiath:AT3G25050.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000412 Genomic DNA. Translation: BAB01890.1 .
CP002686 Genomic DNA. Translation: AEE76972.1 .
DQ446697 mRNA. Translation: ABE65966.1 .
RefSeqi NP_189141.1. NM_113409.4.
UniGenei At.46272.

3D structure databases

ProteinModelPortali Q9LJR7.
SMRi Q9LJR7. Positions 35-289.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbi Q9LJR7.
PRIDEi Q9LJR7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G25050.1 ; AT3G25050.1 ; AT3G25050 .
GeneIDi 822096.
KEGGi ath:AT3G25050.

Organism-specific databases

TAIRi AT3G25050.

Phylogenomic databases

eggNOGi COG2273.
InParanoidi Q9LJR7.
KOi K08235.
OMAi GACESSN.
PhylomeDBi Q9LJR7.

Enzyme and pathway databases

BioCyci ARA:AT3G25050-MONOMER.

Gene expression databases

ExpressionAtlasi Q9LJR7. differential.
Genevestigatori Q9LJR7.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF005604. XET. 1 hit.
PRINTSi PR00737. GLHYDRLASE16.
SUPFAMi SSF49899. SSF49899. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations."
    Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.
    Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
    Yokoyama R., Nishitani K.
    Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  5. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
    Rose J.K.C., Braam J., Fry S.C., Nishitani K.
    Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiXTH3_ARATH
AccessioniPrimary (citable) accession number: Q9LJR7
Secondary accession number(s): Q1PEM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3