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Q9LJR7 (XTH3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Xyloglucan endotransglucosylase/hydrolase protein 3

Short name=At-XTH3
Short name=XTH-3
EC=2.4.1.207
Gene names
Name:XTH3
Ordered Locus Names:At3g25050
ORF Names:K3G3.6
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length290 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Subcellular location

Secretedcell wall Probable. Secretedextracellular spaceapoplast Probable.

Tissue specificity

Predominantly expressed in flower buds. Ref.4

Induction

By auxin and brassinolide. Ref.4

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. XTH group 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 290269Xyloglucan endotransglucosylase/hydrolase protein 3
PRO_0000011803

Sites

Active site1091Nucleophile By similarity
Active site1131Proton donor By similarity

Amino acid modifications

Glycosylation2101N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9LJR7 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 5139E94737955AF0

FASTA29033,259
        10         20         30         40         50         60 
MDYMRIFSVF VVTLWIIRVD ARVFGGRGIE KFVTFGQNYI VTWGQSHVST LHSGEEVDLY 

        70         80         90        100        110        120 
MDQSSGGGFE SKDAYGSGLF EMRIKVPSGN TGGIVTAFYL TSKGGGHDEI DFEFLGNNNG 

       130        140        150        160        170        180 
KPVTLQTNLF LNGEGNREER FLLWFNPTKH YHTYGLLWNP YQIVFYVDNI PIRVYKNENG 

       190        200        210        220        230        240 
VSYPSKPMQV EASLWNGDDW ATDGGRTKVN WSYSPFIAHF RDFALSGCNI DGRSNNVGAC 

       250        260        270        280        290 
ESSNYWWNAG NYQRLSGNEQ KLYEHVRSKY MNYDYCTDRS KYQTPPRECY 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations."
Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.
Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
Yokoyama R., Nishitani K.
Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
[5]"The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
Rose J.K.C., Braam J., Fry S.C., Nishitani K.
Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP000412 Genomic DNA. Translation: BAB01890.1.
CP002686 Genomic DNA. Translation: AEE76972.1.
DQ446697 mRNA. Translation: ABE65966.1.
RefSeqNP_189141.1. NM_113409.4.
UniGeneAt.46272.

3D structure databases

ProteinModelPortalQ9LJR7.
SMRQ9LJR7. Positions 33-290.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbQ9LJR7.
PRIDEQ9LJR7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G25050.1; AT3G25050.1; AT3G25050.
GeneID822096.
KEGGath:AT3G25050.

Organism-specific databases

TAIRAT3G25050.

Phylogenomic databases

eggNOGCOG2273.
InParanoidQ9LJR7.
KOK08235.
OMAGACESSN.
PhylomeDBQ9LJR7.
ProtClustDBCLSN2915354.

Enzyme and pathway databases

BioCycARA:AT3G25050-MONOMER.

Gene expression databases

GenevestigatorQ9LJR7.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008264. Beta_glucanase.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
PRINTSPR00737. GLHYDRLASE16.
SUPFAMSSF49899. SSF49899. 1 hit.
ProtoNetSearch...

Entry information

Entry nameXTH3_ARATH
AccessionPrimary (citable) accession number: Q9LJR7
Secondary accession number(s): Q1PEM0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names