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Protein

Protein SPA1-RELATED 3

Gene

SPA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Repressor of photomorphogenesis in the light. Probably part of the COP1/SPA E3 ubiquitin-protein ligase complex.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Phytochrome signaling pathway, Ubl conjugation pathway

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SPA1-RELATED 3
Gene namesi
Name:SPA3
Ordered Locus Names:At3g15354
ORF Names:K7L4.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G15354.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003634931 – 845Protein SPA1-RELATED 3Add BLAST845

Proteomic databases

PaxDbiQ9LJR3.
PRIDEiQ9LJR3.

PTM databases

iPTMnetiQ9LJR3.

Expressioni

Inductioni

Up-regulated by red, far-red and blue light.2 Publications

Interactioni

Subunit structurei

Interacts with COP1 and CO.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-626921,EBI-626921
COL1O500557EBI-626921,EBI-1112154
COP1P432547EBI-626921,EBI-301649
SPA1Q9SYX27EBI-626921,EBI-626992
SPA4Q94BM76EBI-626921,EBI-626943

Protein-protein interaction databases

BioGridi6101. 11 interactors.
IntActiQ9LJR3. 4 interactors.
STRINGi3702.AT3G15354.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LJR3.
SMRiQ9LJR3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 297Protein kinasePROSITE-ProRule annotationAdd BLAST297
Repeati532 – 571WD 1Add BLAST40
Repeati581 – 621WD 2Add BLAST41
Repeati624 – 664WD 3Add BLAST41
Repeati666 – 706WD 4Add BLAST41
Repeati710 – 748WD 5Add BLAST39
Repeati757 – 796WD 6Add BLAST40
Repeati812 – 845WD 7Add BLAST34

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili301 – 329Sequence analysisAdd BLAST29

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi685 – 699DWD boxAdd BLAST15

Domaini

The protein kinase domain is predicted to be catalytically inactive. The DWD box is required for interaction with DDB1A (By similarity).PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1033. Eukaryota.
ENOG410XS0B. LUCA.
HOGENOMiHOG000029775.
InParanoidiQ9LJR3.
OrthoDBiEOG093602MQ.
PhylomeDBiQ9LJR3.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00220. S_TKc. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LJR3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGSSNSNSR GFNTSGVSDR NTEFLPVERL TTRSKPSSHV DEYVRSLFGS
60 70 80 90 100
TSTHKSGEDD SLGIDPFVRS LEWGDVSLRQ WLDKPERSVD VFECLHVFRQ
110 120 130 140 150
IVEIVNAAHS QGIVVHNVRP SCFVMSSFNH VSFIESASCS DSGSDSLEDG
160 170 180 190 200
PISQKEIGSS RREEAVSKAI AIEEKGVYNK LLERKIEKLE EEKTQPFPMK
210 220 230 240 250
HILAMETSWY TSPEEDFGSS STCASDVYRL GVLLFELFCP VPSREEKSRT
260 270 280 290 300
MSSLRHRVLP PQILLKCPKE ASFCLWLLHP EPTCRPSMSD LLQSEFITEP
310 320 330 340 350
RDNLEEREAA IELRDRIEEQ ESLLEFLLLI QQRKQESAYR LQDTVSLLSS
360 370 380 390 400
DIEQVVKRQL ILKKRGSSLS DFSKDDHQYT SGQPLMSFQA NEEPSAFLAS
410 420 430 440 450
RKRVRQGILA LENGVEVDEE SQGSTLLESS RLMRNFKKLE SVYFLTRRRQ
460 470 480 490 500
MKAAASGKSL TRHSPLSSEN GRGSMIVSEK SSVSNPVAPK AFFNNDSRQG
510 520 530 540 550
GWIDPFLEGL CRYLSFSQLR VKADLKQGDL LNSSNLVCAL AFDREGELFA
560 570 580 590 600
TAGVNKKIKI FECNSIVNDN RDIHYPVVEL AGRSKLSSLC WNSYIKSQIA
610 620 630 640 650
SSNFDGVVQI WDVARSQLVT EMKEHKKRVW SIDISSADPT LLASGSDDGT
660 670 680 690 700
VKLWSINQGV SIGTIKTKAN VCCVQFPSDS GRSLAFGSAD HKVYYYDLRN
710 720 730 740 750
PKIPLCTMIG HSKTVSYVKF VDSSTLVSSS TDNTLKLWDL SMSASGINES
760 770 780 790 800
PLHSFTGHTN LKNFVGLSVS DGYIATGSET NEVFVYHKAF PMPVMSYMFN
810 820 830 840
NTDSMSGLEV DDASQFISSI CWRGQSSTLV AANSNGNIKI LEMMT
Length:845
Mass (Da):94,354
Last modified:October 1, 2000 - v1
Checksum:iEB5AADEDFD16F316
GO
Isoform 2 (identifier: Q9LJR3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     659-662: GVSI → AILI
     663-845: Missing.

Note: No experimental confirmation available.
Show »
Length:662
Mass (Da):74,483
Checksum:i1AA54AE09A405603
GO

Sequence cautioni

The sequence AEE75647 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042150659 – 662GVSI → AILI in isoform 2. 1 Publication4
Alternative sequenceiVSP_042151663 – 845Missing in isoform 2. 1 PublicationAdd BLAST183

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HQ228994 mRNA. Translation: AEC32932.1.
HQ228995 mRNA. Translation: AEC32933.1.
AP000413 Genomic DNA. Translation: BAB02165.1.
CP002686 Genomic DNA. Translation: AEE75647.1. Sequence problems.
RefSeqiNP_683567.1. NM_148725.4.
UniGeneiAt.1578.
At.71086.

Genome annotation databases

EnsemblPlantsiAT3G15354.1; AT3G15354.1; AT3G15354.
GeneIDi820767.
GrameneiAT3G15354.1; AT3G15354.1; AT3G15354.
KEGGiath:AT3G15354.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HQ228994 mRNA. Translation: AEC32932.1.
HQ228995 mRNA. Translation: AEC32933.1.
AP000413 Genomic DNA. Translation: BAB02165.1.
CP002686 Genomic DNA. Translation: AEE75647.1. Sequence problems.
RefSeqiNP_683567.1. NM_148725.4.
UniGeneiAt.1578.
At.71086.

3D structure databases

ProteinModelPortaliQ9LJR3.
SMRiQ9LJR3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6101. 11 interactors.
IntActiQ9LJR3. 4 interactors.
STRINGi3702.AT3G15354.1.

PTM databases

iPTMnetiQ9LJR3.

Proteomic databases

PaxDbiQ9LJR3.
PRIDEiQ9LJR3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G15354.1; AT3G15354.1; AT3G15354.
GeneIDi820767.
GrameneiAT3G15354.1; AT3G15354.1; AT3G15354.
KEGGiath:AT3G15354.

Organism-specific databases

TAIRiAT3G15354.

Phylogenomic databases

eggNOGiKOG1033. Eukaryota.
ENOG410XS0B. LUCA.
HOGENOMiHOG000029775.
InParanoidiQ9LJR3.
OrthoDBiEOG093602MQ.
PhylomeDBiQ9LJR3.

Enzyme and pathway databases

ReactomeiR-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.

Miscellaneous databases

PROiQ9LJR3.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00220. S_TKc. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPA3_ARATH
AccessioniPrimary (citable) accession number: Q9LJR3
Secondary accession number(s): F4IYP4, F4ZN82, F4ZN83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.