Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein SPA1-RELATED 3

Gene

SPA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Repressor of photomorphogenesis in the light. Probably part of the COP1/SPA E3 ubiquitin-protein ligase complex.3 Publications

GO - Molecular functioni

  • ATP binding Source: InterPro
  • identical protein binding Source: IntAct
  • protein kinase activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Phytochrome signaling pathway, Ubl conjugation pathway

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SPA1-RELATED 3
Gene namesi
Name:SPA3
Ordered Locus Names:At3g15354
ORF Names:K7L4.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G15354.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 845845Protein SPA1-RELATED 3PRO_0000363493Add
BLAST

Proteomic databases

PaxDbiQ9LJR3.

PTM databases

iPTMnetiQ9LJR3.

Expressioni

Inductioni

Up-regulated by red, far-red and blue light.2 Publications

Interactioni

Subunit structurei

Interacts with COP1 and CO.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-626921,EBI-626921
COL1O500557EBI-626921,EBI-1112154
COP1P432547EBI-626921,EBI-301649
SPA1Q9SYX27EBI-626921,EBI-626992
SPA4Q94BM76EBI-626921,EBI-626943

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi6101. 11 interactions.
IntActiQ9LJR3. 4 interactions.
STRINGi3702.AT3G15354.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LJR3.
SMRiQ9LJR3. Positions 207-294, 526-838.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 297297Protein kinasePROSITE-ProRule annotationAdd
BLAST
Repeati532 – 57140WD 1Add
BLAST
Repeati581 – 62141WD 2Add
BLAST
Repeati624 – 66441WD 3Add
BLAST
Repeati666 – 70641WD 4Add
BLAST
Repeati710 – 74839WD 5Add
BLAST
Repeati757 – 79640WD 6Add
BLAST
Repeati812 – 84534WD 7Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili301 – 32929Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi685 – 69915DWD boxAdd
BLAST

Domaini

The protein kinase domain is predicted to be catalytically inactive. The DWD box is required for interaction with DDB1A (By similarity).PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1033. Eukaryota.
ENOG410XS0B. LUCA.
HOGENOMiHOG000029775.
InParanoidiQ9LJR3.
PhylomeDBiQ9LJR3.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00220. S_TKc. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LJR3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGSSNSNSR GFNTSGVSDR NTEFLPVERL TTRSKPSSHV DEYVRSLFGS
60 70 80 90 100
TSTHKSGEDD SLGIDPFVRS LEWGDVSLRQ WLDKPERSVD VFECLHVFRQ
110 120 130 140 150
IVEIVNAAHS QGIVVHNVRP SCFVMSSFNH VSFIESASCS DSGSDSLEDG
160 170 180 190 200
PISQKEIGSS RREEAVSKAI AIEEKGVYNK LLERKIEKLE EEKTQPFPMK
210 220 230 240 250
HILAMETSWY TSPEEDFGSS STCASDVYRL GVLLFELFCP VPSREEKSRT
260 270 280 290 300
MSSLRHRVLP PQILLKCPKE ASFCLWLLHP EPTCRPSMSD LLQSEFITEP
310 320 330 340 350
RDNLEEREAA IELRDRIEEQ ESLLEFLLLI QQRKQESAYR LQDTVSLLSS
360 370 380 390 400
DIEQVVKRQL ILKKRGSSLS DFSKDDHQYT SGQPLMSFQA NEEPSAFLAS
410 420 430 440 450
RKRVRQGILA LENGVEVDEE SQGSTLLESS RLMRNFKKLE SVYFLTRRRQ
460 470 480 490 500
MKAAASGKSL TRHSPLSSEN GRGSMIVSEK SSVSNPVAPK AFFNNDSRQG
510 520 530 540 550
GWIDPFLEGL CRYLSFSQLR VKADLKQGDL LNSSNLVCAL AFDREGELFA
560 570 580 590 600
TAGVNKKIKI FECNSIVNDN RDIHYPVVEL AGRSKLSSLC WNSYIKSQIA
610 620 630 640 650
SSNFDGVVQI WDVARSQLVT EMKEHKKRVW SIDISSADPT LLASGSDDGT
660 670 680 690 700
VKLWSINQGV SIGTIKTKAN VCCVQFPSDS GRSLAFGSAD HKVYYYDLRN
710 720 730 740 750
PKIPLCTMIG HSKTVSYVKF VDSSTLVSSS TDNTLKLWDL SMSASGINES
760 770 780 790 800
PLHSFTGHTN LKNFVGLSVS DGYIATGSET NEVFVYHKAF PMPVMSYMFN
810 820 830 840
NTDSMSGLEV DDASQFISSI CWRGQSSTLV AANSNGNIKI LEMMT
Length:845
Mass (Da):94,354
Last modified:October 1, 2000 - v1
Checksum:iEB5AADEDFD16F316
GO
Isoform 2 (identifier: Q9LJR3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     659-662: GVSI → AILI
     663-845: Missing.

Note: No experimental confirmation available.
Show »
Length:662
Mass (Da):74,483
Checksum:i1AA54AE09A405603
GO

Sequence cautioni

The sequence AEE75647.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei659 – 6624GVSI → AILI in isoform 2. 1 PublicationVSP_042150
Alternative sequencei663 – 845183Missing in isoform 2. 1 PublicationVSP_042151Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HQ228994 mRNA. Translation: AEC32932.1.
HQ228995 mRNA. Translation: AEC32933.1.
AP000413 Genomic DNA. Translation: BAB02165.1.
CP002686 Genomic DNA. Translation: AEE75647.1. Sequence problems.
RefSeqiNP_683567.1. NM_148725.3.
UniGeneiAt.1578.
At.71086.

Genome annotation databases

EnsemblPlantsiAT3G15354.1; AT3G15354.1; AT3G15354.
GeneIDi820767.
GrameneiAT3G15354.1; AT3G15354.1; AT3G15354.
KEGGiath:AT3G15354.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HQ228994 mRNA. Translation: AEC32932.1.
HQ228995 mRNA. Translation: AEC32933.1.
AP000413 Genomic DNA. Translation: BAB02165.1.
CP002686 Genomic DNA. Translation: AEE75647.1. Sequence problems.
RefSeqiNP_683567.1. NM_148725.3.
UniGeneiAt.1578.
At.71086.

3D structure databases

ProteinModelPortaliQ9LJR3.
SMRiQ9LJR3. Positions 207-294, 526-838.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6101. 11 interactions.
IntActiQ9LJR3. 4 interactions.
STRINGi3702.AT3G15354.1.

PTM databases

iPTMnetiQ9LJR3.

Proteomic databases

PaxDbiQ9LJR3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G15354.1; AT3G15354.1; AT3G15354.
GeneIDi820767.
GrameneiAT3G15354.1; AT3G15354.1; AT3G15354.
KEGGiath:AT3G15354.

Organism-specific databases

TAIRiAT3G15354.

Phylogenomic databases

eggNOGiKOG1033. Eukaryota.
ENOG410XS0B. LUCA.
HOGENOMiHOG000029775.
InParanoidiQ9LJR3.
PhylomeDBiQ9LJR3.

Enzyme and pathway databases

ReactomeiR-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.

Miscellaneous databases

PROiQ9LJR3.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00220. S_TKc. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis cryptochrome 1 interacts with SPA1 to suppress COP1 activity in response to blue light."
    Liu B., Zuo Z., Liu H., Liu X., Lin C.
    Genes Dev. 25:1029-1034(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "The SPA1-like proteins SPA3 and SPA4 repress photomorphogenesis in the light."
    Laubinger S., Hoecker U.
    Plant J. 35:373-385(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH COP1, GENE FAMILY, NOMENCLATURE.
  5. "The SPA quartet: a family of WD-repeat proteins with a central role in suppression of photomorphogenesis in Arabidopsis."
    Laubinger S., Fittinghoff K., Hoecker U.
    Plant Cell 16:2293-2306(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  6. "Functional and expression analysis of Arabidopsis SPA genes during seedling photomorphogenesis and adult growth."
    Fittinghoff K., Laubinger S., Nixdorf M., Fackendahl P., Baumgardt R.-L., Batschauer A., Hoecker U.
    Plant J. 47:577-590(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY LIGHT.
  7. "Arabidopsis SPA proteins regulate photoperiodic flowering and interact with the floral inducer CONSTANS to regulate its stability."
    Laubinger S., Marchal V., Le Gourrierec J., Wenkel S., Adrian J., Jang S., Kulajta C., Braun H., Coupland G., Hoecker U.
    Development 133:3213-3222(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CO, INDUCTION.
  8. "Characterization of Arabidopsis and rice DWD proteins and their roles as substrate receptors for CUL4-RING E3 ubiquitin ligases."
    Lee J.H., Terzaghi W., Gusmaroli G., Charron J.B., Yoon H.J., Chen H., He Y.J., Xiong Y., Deng X.W.
    Plant Cell 20:152-167(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DWD MOTIF.

Entry informationi

Entry nameiSPA3_ARATH
AccessioniPrimary (citable) accession number: Q9LJR3
Secondary accession number(s): F4IYP4, F4ZN82, F4ZN83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.