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Protein

Protein SPA1-RELATED 3

Gene

SPA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Repressor of photomorphogenesis in the light. Probably part of the COP1/SPA E3 ubiquitin-protein ligase complex.3 Publications

GO - Molecular functioni

  • ATP binding Source: InterPro
  • identical protein binding Source: IntAct
  • protein kinase activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPhytochrome signaling pathway, Ubl conjugation pathway
LigandNucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-ATH-8951664 Neddylation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SPA1-RELATED 3
Gene namesi
Name:SPA3
Ordered Locus Names:At3g15354
ORF Names:K7L4.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G15354

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003634931 – 845Protein SPA1-RELATED 3Add BLAST845

Proteomic databases

PaxDbiQ9LJR3

PTM databases

iPTMnetiQ9LJR3

Expressioni

Inductioni

Up-regulated by red, far-red and blue light.2 Publications

Gene expression databases

ExpressionAtlasiQ9LJR3 baseline and differential

Interactioni

Subunit structurei

Interacts with COP1 and CO.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi6101, 11 interactors
IntActiQ9LJR3, 4 interactors
STRINGi3702.AT3G15354.1

Structurei

3D structure databases

ProteinModelPortaliQ9LJR3
SMRiQ9LJR3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 297Protein kinasePROSITE-ProRule annotationAdd BLAST297
Repeati532 – 571WD 1Add BLAST40
Repeati581 – 621WD 2Add BLAST41
Repeati624 – 664WD 3Add BLAST41
Repeati666 – 706WD 4Add BLAST41
Repeati710 – 748WD 5Add BLAST39
Repeati757 – 796WD 6Add BLAST40
Repeati812 – 845WD 7Add BLAST34

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili301 – 329Sequence analysisAdd BLAST29

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi685 – 699DWD boxAdd BLAST15

Domaini

The protein kinase domain is predicted to be catalytically inactive. The DWD box is required for interaction with DDB1A (By similarity).PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1033 Eukaryota
ENOG410XS0B LUCA
HOGENOMiHOG000029775
InParanoidiQ9LJR3
OrthoDBiEOG093602MQ
PhylomeDBiQ9LJR3

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 2 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
SSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00678 WD_REPEATS_1, 2 hits
PS50082 WD_REPEATS_2, 3 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LJR3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGSSNSNSR GFNTSGVSDR NTEFLPVERL TTRSKPSSHV DEYVRSLFGS
60 70 80 90 100
TSTHKSGEDD SLGIDPFVRS LEWGDVSLRQ WLDKPERSVD VFECLHVFRQ
110 120 130 140 150
IVEIVNAAHS QGIVVHNVRP SCFVMSSFNH VSFIESASCS DSGSDSLEDG
160 170 180 190 200
PISQKEIGSS RREEAVSKAI AIEEKGVYNK LLERKIEKLE EEKTQPFPMK
210 220 230 240 250
HILAMETSWY TSPEEDFGSS STCASDVYRL GVLLFELFCP VPSREEKSRT
260 270 280 290 300
MSSLRHRVLP PQILLKCPKE ASFCLWLLHP EPTCRPSMSD LLQSEFITEP
310 320 330 340 350
RDNLEEREAA IELRDRIEEQ ESLLEFLLLI QQRKQESAYR LQDTVSLLSS
360 370 380 390 400
DIEQVVKRQL ILKKRGSSLS DFSKDDHQYT SGQPLMSFQA NEEPSAFLAS
410 420 430 440 450
RKRVRQGILA LENGVEVDEE SQGSTLLESS RLMRNFKKLE SVYFLTRRRQ
460 470 480 490 500
MKAAASGKSL TRHSPLSSEN GRGSMIVSEK SSVSNPVAPK AFFNNDSRQG
510 520 530 540 550
GWIDPFLEGL CRYLSFSQLR VKADLKQGDL LNSSNLVCAL AFDREGELFA
560 570 580 590 600
TAGVNKKIKI FECNSIVNDN RDIHYPVVEL AGRSKLSSLC WNSYIKSQIA
610 620 630 640 650
SSNFDGVVQI WDVARSQLVT EMKEHKKRVW SIDISSADPT LLASGSDDGT
660 670 680 690 700
VKLWSINQGV SIGTIKTKAN VCCVQFPSDS GRSLAFGSAD HKVYYYDLRN
710 720 730 740 750
PKIPLCTMIG HSKTVSYVKF VDSSTLVSSS TDNTLKLWDL SMSASGINES
760 770 780 790 800
PLHSFTGHTN LKNFVGLSVS DGYIATGSET NEVFVYHKAF PMPVMSYMFN
810 820 830 840
NTDSMSGLEV DDASQFISSI CWRGQSSTLV AANSNGNIKI LEMMT
Length:845
Mass (Da):94,354
Last modified:October 1, 2000 - v1
Checksum:iEB5AADEDFD16F316
GO
Isoform 2 (identifier: Q9LJR3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     659-662: GVSI → AILI
     663-845: Missing.

Note: No experimental confirmation available.
Show »
Length:662
Mass (Da):74,483
Checksum:i1AA54AE09A405603
GO

Sequence cautioni

The sequence AEE75647 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042150659 – 662GVSI → AILI in isoform 2. 1 Publication4
Alternative sequenceiVSP_042151663 – 845Missing in isoform 2. 1 PublicationAdd BLAST183

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HQ228994 mRNA Translation: AEC32932.1
HQ228995 mRNA Translation: AEC32933.1
AP000413 Genomic DNA Translation: BAB02165.1
CP002686 Genomic DNA Translation: AEE75647.1 Sequence problems.
CP002686 Genomic DNA Translation: ANM65673.1
CP002686 Genomic DNA Translation: ANM65674.1
RefSeqiNP_001327623.1, NM_001338161.1 [Q9LJR3-2]
NP_001327624.1, NM_001338162.1 [Q9LJR3-1]
NP_683567.1, NM_148725.4
UniGeneiAt.1578
At.71086

Genome annotation databases

EnsemblPlantsiAT3G15354.2; AT3G15354.2; AT3G15354 [Q9LJR3-2]
AT3G15354.3; AT3G15354.3; AT3G15354 [Q9LJR3-1]
GeneIDi820767
GrameneiAT3G15354.2; AT3G15354.2; AT3G15354 [Q9LJR3-2]
AT3G15354.3; AT3G15354.3; AT3G15354 [Q9LJR3-1]
KEGGiath:AT3G15354

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSPA3_ARATH
AccessioniPrimary (citable) accession number: Q9LJR3
Secondary accession number(s): F4IYP4, F4ZN82, F4ZN83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names

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