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Reviewed, UniProtKB/Swiss-Prot Q9LJK2 (ABAH4_ARATH)

Last modified February 9, 2010. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Abscisic acid 8'-hydroxylase 4
      Short name=ABA 8'-hydroxylase 4
    EC=1.14.13.93
Alternative name(s):
    Cytochrome P450 707A4
Gene names
Name: CYP707A4
Ordered Locus Names: At3g19270
ORF Names: MVI11.19
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Involved in the oxidative degradation of abscisic acid, but not in the isomerization of the produced 8'-hydroxyabscisic acid (8'-OH-ABA) to (-)-phaseic acid (PA). Ref.3 Ref.4

Catalytic activity

+-abscisate + NADPH + O2 = 8'-hydroxyabscisate + NADP+ + H2O.

Cofactor

Heme group By similarity.

Pathway

Plant hormone degradation; abscisic acid degradation.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Tissue specificity

Mainly expressed in flowers. Lower expression in siliques, rosette leaves, roots and stems. Not expressed in dry seeds. Expressed in silique envelopes, but not in embryo or endosperm during the seed development. Ref.3 Ref.4

Developmental stage

Not induced after imbibition. Ref.3 Ref.4

Induction

By abscisic acid, dehydration and rehydration. Ref.3

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence caution

The sequence BAB02968.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processStress response
   Cellular componentMembrane
   DomainTransmembrane
   LigandHeme
Iron
Metal-binding
NADP
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

response to stress

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function(+)-abscisic acid 8'-hydroxylase activity

Inferred from direct assay. Source: TAIR

electron carrier activity

Inferred from electronic annotation. Source: InterPro

heme binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 468468Abscisic acid 8'-hydroxylase 4
PRO_0000288642

Regions

Transmembrane4 – 2421 Potential

Sites

Metal binding4151Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9LJK2-1 [UniParc].

Last modified May 29, 2007. Version 2.
Checksum: 0D9FA04292C062C7

FASTA46853,922
        10         20         30         40         50         60 
MAEIWFLVVP ILILCLLLVR VIVSKKKKNS RGKLPPGSMG WPYLGETLQL YSQNPNVFFT 

        70         80         90        100        110        120 
SKQKRYGEIF KTRILGYPCV MLASPEAARF VLVTHAHMFK PTYPRSKEKL IGPSALFFHQ 

       130        140        150        160        170        180 
GDYHSHIRKL VQSSFYPETI RKLIPDIEHI ALSSLQSWAN MPIVSTYQEM KKFAFDVGIL 

       190        200        210        220        230        240 
AIFGHLESSY KEILKHNYNI VDKGYNSFPM SLPGTSYHKA LMARKQLKTI VSEIICERRE 

       250        260        270        280        290        300 
KRALQTDFLG HLLNFKNEKG RVLTQEQIAD NIIGVLFAAQ DTTASCLTWI LKYLHDDQKL 

       310        320        330        340        350        360 
LEAVKAEQKA IYEENSREKK PLTWRQTRNM PLTHKVIVES LRMASIISFT FREAVVDVEY 

       370        380        390        400        410        420 
KGYLIPKGWK VMPLFRNIHH NPKYFSNPEV FDPSRFEVNP KPNTFMPFGS GVHACPGNEL 

       430        440        450        460 
AKLQILIFLH HLVSNFRWEV KGGEKGIQYS PFPIPQNGLP ATFRRHSL 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases: key enzymes in ABA catabolism."
Kushiro T., Okamoto M., Nakabayashi K., Yamagishi K., Kitamura S., Asami T., Hirai N., Koshiba T., Kamiya Y., Nambara E.
EMBO J. 23:1647-1656(2004) [PubMed: 15044947] [Abstract]
Cited for: IDENTIFICATION, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
[4]"CYP707A1 and CYP707A2, which encode abscisic acid 8'-hydroxylases, are indispensable for proper control of seed dormancy and germination in Arabidopsis."
Okamoto M., Kuwahara A., Seo M., Kushiro T., Asami T., Hirai N., Kamiya Y., Koshiba T., Nambara E.
Plant Physiol. 141:97-107(2006) [PubMed: 16543410] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP000419 Genomic DNA. Translation: BAB02968.1. Sequence problems.
AY085068 mRNA. Translation: AAM61624.1.
IPIIPI00518422.
RefSeqNP_566628.1.
UniGeneAt.38423

3D structure databases

SMRQ9LJK2. Positions 33-465.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9LJK2.

Proteomic databases

PRIDEQ9LJK2.

Genome annotation databases

GeneID821461.
GenomeReviewsGene locus AT3G19270 in contig BA000014_GR.
KEGGath:AT3G19270.
NMPDRfig|3702.1.peg.14140.

Organism-specific databases

GeneFarm1254. 94.
TAIRAt3g19270.

Phylogenomic databases

eggNOGKOG0157.
HOGENOMHBG744016.
InParanoidQ9LJK2.
OMAFHAMKKL.
PhylomeDBQ9LJK2.

Enzyme and pathway databases

BRENDA1.14.13.93. 302.

Gene expression databases

GenevestigatorQ9LJK2.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR017973. Cyt_P450_C.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameABAH4_ARATH
AccessionPrimary (citable) accession number: Q9LJK2
Secondary accession number(s): Q8LF37
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: February 9, 2010
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents