Q9LJK2 (ABAH4_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 75.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Abscisic acid 8'-hydroxylase 4 Short name=ABA 8'-hydroxylase 4 EC=1.14.13.93 Alternative name(s): Cytochrome P450 707A4 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Protein attributes
| Sequence length | 468 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Involved in the oxidative degradation of abscisic acid, but not in the isomerization of the produced 8'-hydroxyabscisic acid (8'-OH-ABA) to (-)-phaseic acid (PA). Ref.4 Ref.5 |
| Catalytic activity | +-abscisate + NADPH + O2 = 8'-hydroxyabscisate + NADP+ + H2O. |
| Cofactor | Heme group By similarity. |
| Pathway | |
| Subcellular location | Membrane; Single-pass membrane protein Potential. |
| Tissue specificity | Mainly expressed in flowers. Lower expression in siliques, rosette leaves, roots and stems. Not expressed in dry seeds. Expressed in silique envelopes, but not in embryo or endosperm during the seed development. Ref.4 Ref.5 |
| Developmental stage | |
| Induction | By abscisic acid, dehydration and rehydration. Ref.4 |
| Sequence similarities | Belongs to the cytochrome P450 family. |
| Sequence caution | The sequence BAB02968.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Stress response |
| Cellular component | Membrane |
| Domain | Transmembrane Transmembrane helix |
| Ligand | Heme Iron Metal-binding NADP |
| Molecular function | Monooxygenase Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | response to stress Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | (+)-abscisic acid 8'-hydroxylase activity Inferred from direct assay. Source: TAIR electron carrier activityInferred from electronic annotation. Source: InterPro heme bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [2] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [3] | "Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [4] | "The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases: key enzymes in ABA catabolism." Kushiro T., Okamoto M., Nakabayashi K., Yamagishi K., Kitamura S., Asami T., Hirai N., Koshiba T., Kamiya Y., Nambara E. EMBO J. 23:1647-1656(2004) [PubMed: 15044947] [Abstract] Cited for: IDENTIFICATION, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION. |
| [5] | "CYP707A1 and CYP707A2, which encode abscisic acid 8'-hydroxylases, are indispensable for proper control of seed dormancy and germination in Arabidopsis." Okamoto M., Kuwahara A., Seo M., Kushiro T., Asami T., Hirai N., Kamiya Y., Koshiba T., Nambara E. Plant Physiol. 141:97-107(2006) [PubMed: 16543410] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AP000419 Genomic DNA. Translation: BAB02968.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE76215.1. AY085068 mRNA. Translation: AAM61624.1. |
| IPI | IPI00518422. |
| RefSeq | NP_566628.1. NM_112814.1. |
| UniGene | At.38423. |
3D structure databases | |
| ProteinModelPortal | Q9LJK2. |
| SMR | Q9LJK2. Positions 32-465. |
| ModBase | Search... |
Proteomic databases | |
| PRIDE | Q9LJK2. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT3G19270.1; AT3G19270.1; AT3G19270. |
| GeneID | 821461. |
| GenomeReviews | Gene locus AT3G19270 in contig BA000014_GR. |
| KEGG | ath:AT3G19270. |
| NMPDR | fig|3702.1.peg.14140. |
Organism-specific databases | |
| GeneFarm | 1254. 94. |
| TAIR | At3g19270. |
Phylogenomic databases | |
| eggNOG | KOG0157. |
| GeneTree | EPGT00070000028041. |
| HOGENOM | HBG744016. |
| InParanoid | Q9LJK2. |
| OMA | HKVIVES. |
| PhylomeDB | Q9LJK2. |
| ProtClustDB | CLSN2714514. |
Gene expression databases | |
| Genevestigator | Q9LJK2. |
Family and domain databases | |
| InterPro | IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view] |
| Gene3D | G3DSA:1.10.630.10. Cyt_P450. 1 hit. |
| KO | K09843. |
| Pfam | PF00067. p450. 1 hit. [Graphical view] |
| PRINTS | PR00463. EP450I. PR00385. P450. |
| SUPFAM | SSF48264. Cytochrome_P450. 1 hit. |
| PROSITE | PS00086. CYTOCHROME_P450. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ABAH4_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q9LJK2 Secondary accession number(s): Q8LF37 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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