Reviewed, UniProtKB/Swiss-Prot Q9LJK2 (ABAH4_ARATH)

Names and origin

Protein names

Recommended name:
    Abscisic acid 8'-hydroxylase 4
      Short name=ABA 8'-hydroxylase 4
    EC=1.14.13.93
Alternative name(s):
    Cytochrome P450 707A4

Gene names

Name:	CYP707A4
Ordered Locus Names:	At3g19270
ORF Names:	MVI11.19

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the oxidative degradation of abscisic acid, but not in the isomerization of the produced 8'-hydroxyabscisic acid (8'-OH-ABA) to (-)-phaseic acid (PA). Ref.3 Ref.4
Catalytic activity	+-abscisate + NADPH + O2 = 8'-hydroxyabscisate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Plant hormone degradation; abscisic acid degradation.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Tissue specificity	Mainly expressed in flowers. Lower expression in siliques, rosette leaves, roots and stems. Not expressed in dry seeds. Expressed in silique envelopes, but not in embryo or endosperm during the seed development. Ref.3 Ref.4
Developmental stage	Not induced after imbibition. Ref.3 Ref.4
Induction	By abscisic acid, dehydration and rehydration. Ref.3
Sequence similarities	Belongs to the cytochrome P450 family.
Sequence caution	The sequence BAB02968.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Membrane
Domain	Transmembrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	(+)-abscisic acid 8'-hydroxylase activity Inferred from direct assay. Source: TAIR electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Abscisic acid 8'-hydroxylase 4		PRO_0000288642
Regions
	Transmembrane	4 – 24	21	Potential
Sites
	Metal binding	415	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LJK2-1 [UniParc]. Last modified May 29, 2007. Version 2. Checksum: 0D9FA04292C062C7	FASTA	468	53,922
	10 20 30 40 50 60 MAEIWFLVVP ILILCLLLVR VIVSKKKKNS RGKLPPGSMG WPYLGETLQL YSQNPNVFFT 70 80 90 100 110 120 SKQKRYGEIF KTRILGYPCV MLASPEAARF VLVTHAHMFK PTYPRSKEKL IGPSALFFHQ 130 140 150 160 170 180 GDYHSHIRKL VQSSFYPETI RKLIPDIEHI ALSSLQSWAN MPIVSTYQEM KKFAFDVGIL 190 200 210 220 230 240 AIFGHLESSY KEILKHNYNI VDKGYNSFPM SLPGTSYHKA LMARKQLKTI VSEIICERRE 250 260 270 280 290 300 KRALQTDFLG HLLNFKNEKG RVLTQEQIAD NIIGVLFAAQ DTTASCLTWI LKYLHDDQKL 310 320 330 340 350 360 LEAVKAEQKA IYEENSREKK PLTWRQTRNM PLTHKVIVES LRMASIISFT FREAVVDVEY 370 380 390 400 410 420 KGYLIPKGWK VMPLFRNIHH NPKYFSNPEV FDPSRFEVNP KPNTFMPFGS GVHACPGNEL 430 440 450 460 AKLQILIFLH HLVSNFRWEV KGGEKGIQYS PFPIPQNGLP ATFRRHSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases: key enzymes in ABA catabolism." Kushiro T., Okamoto M., Nakabayashi K., Yamagishi K., Kitamura S., Asami T., Hirai N., Koshiba T., Kamiya Y., Nambara E. EMBO J. 23:1647-1656(2004) [PubMed: 15044947] [Abstract] Cited for: IDENTIFICATION, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
[4]	"CYP707A1 and CYP707A2, which encode abscisic acid 8'-hydroxylases, are indispensable for proper control of seed dormancy and germination in Arabidopsis." Okamoto M., Kuwahara A., Seo M., Kushiro T., Asami T., Hirai N., Kamiya Y., Koshiba T., Nambara E. Plant Physiol. 141:97-107(2006) [PubMed: 16543410] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP000419 Genomic DNA. Translation: BAB02968.1. Sequence problems. AY085068 mRNA. Translation: AAM61624.1.
IPI	IPI00518422.
RefSeq	NP_566628.1.
UniGene	At.38423
3D structure databases
SMR	Q9LJK2. Positions 33-465.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LJK2.
Proteomic databases
PRIDE	Q9LJK2.
Genome annotation databases
GeneID	821461.
GenomeReviews	Gene locus AT3G19270 in contig BA000014_GR.
KEGG	ath:AT3G19270.
NMPDR	fig|3702.1.peg.14140.
Organism-specific databases
GeneFarm	1254. 94.
TAIR	At3g19270.
Phylogenomic databases
eggNOG	KOG0157.
HOGENOM	HBG744016.
InParanoid	Q9LJK2.
OMA	FHAMKKL.
PhylomeDB	Q9LJK2.
Enzyme and pathway databases
BRENDA	1.14.13.93. 302.
Gene expression databases
Genevestigator	Q9LJK2.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABAH4_ARATH

Accession

Primary (citable) accession number: Q9LJK2
Secondary accession number(s): Q8LF37

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	February 9, 2010
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)