Q9LJK1 (FUT11_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycoprotein 3-alpha-L-fucosyltransferase A
EC=2.4.1.214

Alternative name(s):
Core alpha-(1,3)-fucosyltransferase
Fuc-T C3
FucT1
FucTA
Fucosyltransferase 11
Short name=AtFUT11

Gene names

Name:	FUT11
Ordered Locus Names:	At3g19280
ORF Names:	MVI11.20

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in cell wall synthesis. Catalyzes alpha-1,3 glycosidic linkages.
Catalytic activity	GDP-L-fucose + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)asparagine = GDP + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-(alpha-L-fucosyl-(1->3))-N-acetyl-beta-D-glucosaminyl)asparagine.
Cofactor	Manganese.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Sequence similarities	Belongs to the glycosyltransferase 10 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-fucose catabolic process Non-traceable author statement. Source: UniProtKB cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW protein N-linked glycosylation Inferred from direct assay. Source: TAIR
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycoprotein 3-alpha-L-fucosyltransferase activity Traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Glycoprotein 3-alpha-L-fucosyltransferase A		PRO_0000221124
Regions
	Topological domain	1 – 39	39	Cytoplasmic Potential
	Transmembrane	40 – 60	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	61 – 501	441	Lumenal Potential
	Compositional bias	26 – 35	10	Poly-Ser
Amino acid modifications
	Glycosylation	64	1	N-linked (GlcNAc...) Potential
	Glycosylation	337	1	N-linked (GlcNAc...) Potential
	Glycosylation	420	1	N-linked (GlcNAc...) Potential
	Glycosylation	481	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	210	1	K → E in AAM68912. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9LJK1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 2CEAC85CD1A3DA5B	FASTA	501	56,205
	10 20 30 40 50 60 MGVFSNLRGP KIGLTHEELP VVANGSTSSS SSPSSFKRKV STFLPICVAL VVIIEIGFLC 70 80 90 100 110 120 RLDNASLVDT LTHFFTKSSS DLKVGSGIEK CQEWLERVDS VTYSRDFTKD PIFISGSNKD 130 140 150 160 170 180 FKSCSVDCVM GFTSDKKPDA AFGLSHQPGT LSIIRSMESA QYYQENNLAQ ARRKGYDIVM 190 200 210 220 230 240 TTSLSSDVPV GYFSWAEYDI MAPVQPKTEK ALAAAFISNC AARNFRLQAL EALMKTNVKI 250 260 270 280 290 300 DSYGGCHRNR DGSVEKVEAL KHYKFSLAFE NTNEEDYVTE KFFQSLVAGS VPVVVGAPNI 310 320 330 340 350 360 EEFAPSPDSF LHIKQMDDVK AVAKKMKYLA DNPDAYNQTL RWKHEGPSDS FKALIDMAAV 370 380 390 400 410 420 HSSCRLCIFV ATRIREQEEK SPEFKRRPCK CTRGSETVYH LYVRERGRFD MESIFLKDGN 430 440 450 460 470 480 LTLEALESAV LAKFMSLRYE PIWKKERPAS LRGDGKLRVH GIYPIGLTQR QALYNFKFEG 490 500 NSSLSTHIQR NPCPKFEVVF V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of cDNAs encoding alpha1,3-fucosyltransferase homologues from Arabidopsis thaliana." Wilson I.B., Rendic D., Freilinger A., Dumic J., Altmann F., Mucha J., Muller S., Hauser M.T. Biochim. Biophys. Acta 1527:88-96(2001) [PubMed: 11420147] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Root.
[2]	"Plant members of the alpha1-->3/4-fucosyltransferase gene family encode an alpha1-->4-fucosyltransferase, potentially involved in Lewis(a) biosynthesis, and two core alpha1-->3-fucosyltransferases." Bakker H., Schijlen E., de Vries T., Schiphorst W.E., Jordi W., Lommen A., Bosch D., van Die I. FEBS Lett. 507:307-312(2001) [PubMed: 11696361] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Silique.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	Kiefer-Meyer M.-C., Faye L., Gomord V. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 26-287. Strain: cv. Wassilewskija.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ404860 mRNA. Translation: CAC38048.1. AJ345084 mRNA. Translation: CAC78979.1. AP000419 Genomic DNA. Translation: BAB02969.1. CP002686 Genomic DNA. Translation: AEE76217.1. AF277228 mRNA. Translation: AAM68912.1. AF277229 mRNA. Translation: AAM68913.1.
IPI	IPI00538014.
RefSeq	NP_188559.1. NM_112815.3.
UniGene	At.8239.
3D structure databases
ProteinModelPortal	Q9LJK1.
SMR	Q9LJK1. Positions 203-359.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LJK1.
Protein family/group databases
CAZy	GT10. Glycosyltransferase Family 10.
Proteomic databases
PRIDE	Q9LJK1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G19280.1; AT3G19280.1; AT3G19280.
GeneID	821462.
GenomeReviews	Gene locus AT3G19280 in contig BA000014_GR.
KEGG	ath:AT3G19280.
NMPDR	fig|3702.1.peg.14141.
Organism-specific databases
TAIR	At3g19280.
Phylogenomic databases
GeneTree	EPGT00070000030315.
HOGENOM	HBG317569.
InParanoid	Q9LJK1.
OMA	ATRIREQ.
PhylomeDB	Q9LJK1.
ProtClustDB	CLSN2682683.
Enzyme and pathway databases
BioCyc	ARA:AT3G19280-MONOMER. MetaCyc:AT3G19280-MONOMER.
Gene expression databases
ArrayExpress	Q9LJK1.
Genevestigator	Q9LJK1.
GermOnline	AT3G19280. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001503. Glyco_trans_10. [Graphical view]
KO	K00753.
PANTHER	PTHR11929. Glyco_trans_10. 1 hit.
Pfam	PF00852. Glyco_transf_10. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUT11_ARATH

Accession

Primary (citable) accession number: Q9LJK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	October 1, 2000
Last modified:	November 16, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program