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Protein

Peroxisomal (S)-2-hydroxy-acid oxidase GLO4

Gene

GLO4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

Cofactori

FMNPROSITE-ProRule annotation

Pathway: photorespiration

This protein is involved in step 2 of the subpathway that synthesizes glycine from 2-phosphoglycolate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 (GLO5), Peroxisomal (S)-2-hydroxy-acid oxidase GLO2 (GLO2), Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 (GLO3), Peroxisomal (S)-2-hydroxy-acid oxidase GLO1 (GLO1), Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 (GLO4)
  3. no protein annotated in this organism
This subpathway is part of the pathway photorespiration, which is itself part of Photosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from 2-phosphoglycolate, the pathway photorespiration and in Photosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei107 – 1071FMNPROSITE-ProRule annotation
Binding sitei128 – 1281FMNPROSITE-ProRule annotation
Binding sitei130 – 1301SubstratePROSITE-ProRule annotation
Binding sitei156 – 1561FMNPROSITE-ProRule annotation
Binding sitei165 – 1651SubstratePROSITE-ProRule annotation
Binding sitei228 – 2281FMNPROSITE-ProRule annotation
Active sitei252 – 2521Proton acceptorPROSITE-ProRule annotation
Binding sitei255 – 2551SubstratePROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi283 – 30725FMNPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • defense response to bacterium Source: TAIR
  • hydrogen peroxide biosynthetic process Source: TAIR
  • oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolate pathway, Photorespiration

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

BioCyciARA:AT3G14130-MONOMER.
ReactomeiREACT_278920. Glyoxylate metabolism.
UniPathwayiUPA00951; UER00912.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 (EC:1.1.3.15)
Alternative name(s):
Glycolate oxidase 4
Short name:
AtGLO4
Short name:
GOX 4
Short chain alpha-hydroxy acid oxidase GLO4
Gene namesi
Name:GLO4
Ordered Locus Names:At3g14130
ORF Names:MAG2.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G14130.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 363363Peroxisomal (S)-2-hydroxy-acid oxidase GLO4PRO_0000403407Add
BLAST

Proteomic databases

PaxDbiQ9LJH5.
PRIDEiQ9LJH5.

Interactioni

Subunit structurei

Homotetramer or homooctamer.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G14130.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LJH5.
SMRiQ9LJH5. Positions 3-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 357357FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi361 – 3633Microbody targeting signalSequence Analysis

Sequence similaritiesi

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
Contains 1 FMN hydroxy acid dehydrogenase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1304.
HOGENOMiHOG000217463.
InParanoidiQ9LJH5.
KOiK11517.
OMAiTYGSTSV.
PhylomeDBiQ9LJH5.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view]
PfamiPF01070. FMN_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LJH5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQIVNVDEF QELAKQALPK MYYDFYNGGA EDQHTLNENV QAFRRIMFRP
60 70 80 90 100
RVLVDVSNID MSTSMLGYPI SAPIMIAPTA MHKLAHPKGE IATAKAAAAC
110 120 130 140 150
NTIMIVSFMS TCTIEEVASS CNAVRFLQIY VYKRRDVTAQ IVKRAEKAGF
160 170 180 190 200
KAIVLTVDVP RLGRREADIK NKMISPQLKN FEGLVSTEVR PNEGSGVEAF
210 220 230 240 250
ASSAFDASLS WKDIEWLRSI TKLPILVKGL LTREDALKAV EAGVDGIVVS
260 270 280 290 300
NHGARQLDYS PATITVLEEV VHAVKGRIPV LLDGGVRRGT DVFKALALGA
310 320 330 340 350
QAVLIGRPIV YGLAAKGEDG VKKVIDMLKN EFEITMALSG CPTIDDVTRN
360
HVRTENERIK SML
Length:363
Mass (Da):39,878
Last modified:October 1, 2000 - v1
Checksum:i19A62CDD8F9E1088
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651M → I in AAM61594 (Ref. 4) Curated
Sequence conflicti107 – 1071S → P in AAM61594 (Ref. 4) Curated
Sequence conflicti273 – 2731A → V in AAM61594 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000600 Genomic DNA. Translation: BAB02977.1.
CP002686 Genomic DNA. Translation: AEE75475.1.
BT002739 mRNA. Translation: AAO22568.1.
AY085037 mRNA. Translation: AAM61594.1.
RefSeqiNP_188029.1. NM_112269.3.
UniGeneiAt.39288.
At.42696.

Genome annotation databases

EnsemblPlantsiAT3G14130.1; AT3G14130.1; AT3G14130.
GeneIDi820630.
KEGGiath:AT3G14130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000600 Genomic DNA. Translation: BAB02977.1.
CP002686 Genomic DNA. Translation: AEE75475.1.
BT002739 mRNA. Translation: AAO22568.1.
AY085037 mRNA. Translation: AAM61594.1.
RefSeqiNP_188029.1. NM_112269.3.
UniGeneiAt.39288.
At.42696.

3D structure databases

ProteinModelPortaliQ9LJH5.
SMRiQ9LJH5. Positions 3-355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G14130.1.

Proteomic databases

PaxDbiQ9LJH5.
PRIDEiQ9LJH5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G14130.1; AT3G14130.1; AT3G14130.
GeneIDi820630.
KEGGiath:AT3G14130.

Organism-specific databases

TAIRiAT3G14130.

Phylogenomic databases

eggNOGiCOG1304.
HOGENOMiHOG000217463.
InParanoidiQ9LJH5.
KOiK11517.
OMAiTYGSTSV.
PhylomeDBiQ9LJH5.

Enzyme and pathway databases

UniPathwayiUPA00951; UER00912.
BioCyciARA:AT3G14130-MONOMER.
ReactomeiREACT_278920. Glyoxylate metabolism.

Miscellaneous databases

PROiQ9LJH5.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view]
PfamiPF01070. FMN_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Inducible antisense suppression of glycolate oxidase reveals its strong regulation over photosynthesis in rice."
    Xu H.-W., Zhang J., Zeng J., Jiang L., Liu E., Peng C., He Z.-H., Peng X.-X.
    J. Exp. Bot. 60:1799-1809(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiGLO4_ARATH
AccessioniPrimary (citable) accession number: Q9LJH5
Secondary accession number(s): Q8LF60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: October 1, 2000
Last modified: June 24, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.