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Protein

Peroxisomal (S)-2-hydroxy-acid oxidase GLO4

Gene

GLO4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

Cofactori

FMNPROSITE-ProRule annotation

Pathwayi: photorespiration

This protein is involved in step 2 of the subpathway that synthesizes glycine from 2-phosphoglycolate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 (GLO5), (S)-2-hydroxy-acid oxidase GLO2 (GLO2), Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 (GLO3), (S)-2-hydroxy-acid oxidase GLO1 (GLO1), Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 (GLO4)
  3. no protein annotated in this organism
This subpathway is part of the pathway photorespiration, which is itself part of Photosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from 2-phosphoglycolate, the pathway photorespiration and in Photosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107FMNPROSITE-ProRule annotation1
Binding sitei128FMNPROSITE-ProRule annotation1
Binding sitei130SubstratePROSITE-ProRule annotation1
Binding sitei156FMNPROSITE-ProRule annotation1
Binding sitei165SubstratePROSITE-ProRule annotation1
Binding sitei228FMNPROSITE-ProRule annotation1
Active sitei252Proton acceptorPROSITE-ProRule annotation1
Binding sitei255SubstratePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi283 – 307FMNPROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • defense response to bacterium Source: TAIR
  • hydrogen peroxide biosynthetic process Source: TAIR
  • oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolate pathway, Photorespiration
LigandFlavoprotein, FMN

Enzyme and pathway databases

BioCyciARA:AT3G14130-MONOMER
ReactomeiR-ATH-389661 Glyoxylate metabolism and glycine degradation
R-ATH-390918 Peroxisomal lipid metabolism
UniPathwayiUPA00951; UER00912

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 (EC:1.1.3.15)
Alternative name(s):
Glycolate oxidase 4
Short name:
AtGLO4
Short name:
GOX 4
Short chain alpha-hydroxy acid oxidase GLO4
Gene namesi
Name:GLO4
Ordered Locus Names:At3g14130
ORF Names:MAG2.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G14130
TAIRilocus:2087487 AT3G14130

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004034071 – 363Peroxisomal (S)-2-hydroxy-acid oxidase GLO4Add BLAST363

Proteomic databases

PaxDbiQ9LJH5

Expressioni

Gene expression databases

ExpressionAtlasiQ9LJH5 baseline and differential
GenevisibleiQ9LJH5 AT

Interactioni

Subunit structurei

Homotetramer or homooctamer.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G14130.1

Structurei

3D structure databases

ProteinModelPortaliQ9LJH5
SMRiQ9LJH5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 357FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd BLAST357

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi361 – 363Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0538 Eukaryota
COG1304 LUCA
HOGENOMiHOG000217463
InParanoidiQ9LJH5
KOiK11517
OMAiNEFHTSM
OrthoDBiEOG09360EDE
PhylomeDBiQ9LJH5

Family and domain databases

CDDicd02809 alpha_hydroxyacid_oxid_FMN, 1 hit
Gene3Di3.20.20.701 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR012133 Alpha-hydoxy_acid_DH_FMN
IPR000262 FMN-dep_DH
IPR037396 FMN_HAD
IPR008259 FMN_hydac_DH_AS
PfamiView protein in Pfam
PF01070 FMN_dh, 1 hit
PIRSFiPIRSF000138 Al-hdrx_acd_dh, 1 hit
PROSITEiView protein in PROSITE
PS00557 FMN_HYDROXY_ACID_DH_1, 1 hit
PS51349 FMN_HYDROXY_ACID_DH_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LJH5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQIVNVDEF QELAKQALPK MYYDFYNGGA EDQHTLNENV QAFRRIMFRP
60 70 80 90 100
RVLVDVSNID MSTSMLGYPI SAPIMIAPTA MHKLAHPKGE IATAKAAAAC
110 120 130 140 150
NTIMIVSFMS TCTIEEVASS CNAVRFLQIY VYKRRDVTAQ IVKRAEKAGF
160 170 180 190 200
KAIVLTVDVP RLGRREADIK NKMISPQLKN FEGLVSTEVR PNEGSGVEAF
210 220 230 240 250
ASSAFDASLS WKDIEWLRSI TKLPILVKGL LTREDALKAV EAGVDGIVVS
260 270 280 290 300
NHGARQLDYS PATITVLEEV VHAVKGRIPV LLDGGVRRGT DVFKALALGA
310 320 330 340 350
QAVLIGRPIV YGLAAKGEDG VKKVIDMLKN EFEITMALSG CPTIDDVTRN
360
HVRTENERIK SML
Length:363
Mass (Da):39,878
Last modified:October 1, 2000 - v1
Checksum:i19A62CDD8F9E1088
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65M → I in AAM61594 (Ref. 4) Curated1
Sequence conflicti107S → P in AAM61594 (Ref. 4) Curated1
Sequence conflicti273A → V in AAM61594 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000600 Genomic DNA Translation: BAB02977.1
CP002686 Genomic DNA Translation: AEE75475.1
CP002686 Genomic DNA Translation: ANM65219.1
BT002739 mRNA Translation: AAO22568.1
AY085037 mRNA Translation: AAM61594.1
RefSeqiNP_001327205.1, NM_001338103.1
NP_188029.1, NM_112269.3
UniGeneiAt.39288
At.42696

Genome annotation databases

EnsemblPlantsiAT3G14130.1; AT3G14130.1; AT3G14130
AT3G14130.3; AT3G14130.3; AT3G14130
GeneIDi820630
GrameneiAT3G14130.1; AT3G14130.1; AT3G14130
AT3G14130.3; AT3G14130.3; AT3G14130
KEGGiath:AT3G14130

Similar proteinsi

Entry informationi

Entry nameiGLO4_ARATH
AccessioniPrimary (citable) accession number: Q9LJH5
Secondary accession number(s): Q8LF60
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome