Reviewed, UniProtKB/Swiss-Prot Q9LJ42 (PEL10_ARATH)

Names and origin

Protein names

Recommended name:
    Probable pectate lyase 10
    EC=4.2.2.2

Gene names

Ordered Locus Names:	At3g24670
ORF Names:	MSD24_7, MSD24.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion. Required for its activity By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.
Sequence caution	The sequence BAB01216.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 440	412	Probable pectate lyase 10		PRO_0000024874
Sites
	Active site	318	1	Potential
	Metal binding	238	1	Calcium By similarity
	Metal binding	262	1	Calcium By similarity
	Metal binding	266	1	Calcium By similarity
Amino acid modifications
	Glycosylation	41	1	N-linked (GlcNAc...) Potential
	Glycosylation	76	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9LJ42-1 [UniParc]. Last modified June 16, 2003. Version 2. Checksum: 5904FD25F5798FDD	FASTA	440	48,761
	10 20 30 40 50 60 MVIFSRSFLA LSTTLIILAL CINSSTMAQE TEDLNSHSSS NSSTANKLPN DDGAWNEHAV 70 80 90 100 110 120 KNPEEVAAMV DMKIKNSTER RRLGFFSCAT GNPIDDCWRC DRNWHLRRKR LANCAIGFGR 130 140 150 160 170 180 NAIGGRDGRY YVVTDPSDHD AVNPRPGTLR HAVIQDRPLW IVFKRDMVIT LTQELIMNSF 190 200 210 220 230 240 KTIDGRGVNV AIAGGACITI QYVTNIIIHG INVHDCRRTG NAMVRSSPSH YGWRTMADGD 250 260 270 280 290 300 AISIFGSSHI WIDHNSLSNC ADGLIDAIMG STAITISNNY MTHHNEVMLM GHSDSYTRDK 310 320 330 340 350 360 LMQVTIAYNH FGEGLIQRMP RCRHGYFHVV NNDYTHWVMY AIGGSANPTI NSQGNRFLAP 370 380 390 400 410 420 GNPFAKEVTK RVGSWQGEWK QWNWRSQGDL MLNGAYFTKS GAAAPASYAR ASSLGAKPAS 430 440 VVSMLTYSSG ALKCRIGMRC

References

[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 94-440. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP000740 Genomic DNA. Translation: BAB01216.1. Sequence problems. AK118210 mRNA. Translation: BAC42832.1. Different initiation.
IPI	IPI00546851.
RefSeq	NP_189110.1.
UniGene	At.43520
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Genome annotation databases
GeneID	822063.
GenomeReviews	Gene locus AT3G24670 in contig BA000014_GR.
KEGG	ath:AT3G24670.
NMPDR	fig|3702.1.peg.14735.
Organism-specific databases
TAIR	At3g24670.
Phylogenomic databases
OMA	ASHIWID.
Enzyme and pathway databases
BRENDA	4.2.2.2. 302.
Gene expression databases
ArrayExpress	Q9LJ42.
Genevestigator	Q9LJ42.
GermOnline	AT3G24670. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. SM00710. PbH1. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEL10_ARATH

Accession

Primary (citable) accession number: Q9LJ42
Secondary accession number(s): Q8GXJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	June 16, 2003
Last modified:	November 3, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)