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Protein

UDP-glucuronate 4-epimerase 6

Gene

GAE6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the negatively charged monosaccharide that forms the backbone of pectic cell wall components.1 Publication

Catalytic activityi

UDP-glucuronate = UDP-D-galacturonate.

Kineticsi

No activity with UDP-Galactose, UDP- Glucose or UDP-Xylose.
  1. KM=0.23 mM for UDP-glucuronate1 Publication

    pH dependencei

    Optimum pH is 7.9. Active from pH 6 to 8.9.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei263Proton acceptorBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi113 – 144NADBy similarityAdd BLAST32

    GO - Molecular functioni

    GO - Biological processi

    • defense response to fungus Source: TAIR
    • defense response to Gram-negative bacterium Source: TAIR
    • galacturonate biosynthetic process Source: TAIR

    Keywordsi

    Molecular functionIsomerase
    Biological processCarbohydrate metabolism
    LigandNAD

    Enzyme and pathway databases

    BRENDAi5.1.3.6 399

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucuronate 4-epimerase 6 (EC:5.1.3.6)
    Alternative name(s):
    UDP-glucuronic acid epimerase 6
    Short name:
    AtUGlcAE2
    Gene namesi
    Name:GAE6
    Synonyms:UGlcAE2
    Ordered Locus Names:At3g23820
    ORF Names:F14O13.1
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 3

    Organism-specific databases

    AraportiAT3G23820
    TAIRilocus:2076066 AT3G23820

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei41 – 61HelicalSequence analysisAdd BLAST21
    Transmembranei111 – 131HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002926011 – 460UDP-glucuronate 4-epimerase 6Add BLAST460

    Proteomic databases

    PaxDbiQ9LIS3
    PRIDEiQ9LIS3

    PTM databases

    iPTMnetiQ9LIS3

    Expressioni

    Tissue specificityi

    In roots, leaf veins, siliques, flowers, pollen and stems.3 Publications

    Gene expression databases

    ExpressionAtlasiQ9LIS3 baseline and differential
    GenevisibleiQ9LIS3 AT

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CPK4Q388694EBI-2297116,EBI-979475

    Protein-protein interaction databases

    BioGridi7297, 1 interactor
    IntActiQ9LIS3, 1 interactor
    STRINGi3702.AT3G23820.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LIS3
    SMRiQ9LIS3
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi65 – 68Poly-Ala4
    Compositional biasi75 – 90Ser-richAdd BLAST16
    Compositional biasi237 – 240Poly-Ser4

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG410IU3F Eukaryota
    COG0451 LUCA
    HOGENOMiHOG000168000
    InParanoidiQ9LIS3
    KOiK08679
    OMAiFFFTINY
    OrthoDBiEOG09360AZR
    PhylomeDBiQ9LIS3

    Family and domain databases

    InterProiView protein in InterPro
    IPR016040 NAD(P)-bd_dom
    IPR036291 NAD(P)-bd_dom_sf
    IPR008089 Nuc_sugar_epim
    PfamiView protein in Pfam
    PF16363 GDP_Man_Dehyd, 1 hit
    PRINTSiPR01713 NUCEPIMERASE
    SUPFAMiSSF51735 SSF51735, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9LIS3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPLSATADTS KTVKLERYNS YLRKIHSTKV LNASSKVLFR ATLLVALVLV
    60 70 80 90 100
    LIFAINYPPL SDSRAAAAHH LHRRSFLSTG LFSSSSSSSS IGGAAWEKRV
    110 120 130 140 150
    RQSSTAKRPH GLSVLVTGAA GFVGSHCSLA LRKRGDGVLG FDNFNDYYDP
    160 170 180 190 200
    SLKRARQELL EKQQVFIVEG DLNDGPLLRK LFDVVPFTHI LHLAAQAGVR
    210 220 230 240 250
    YAMKNPQSYI ASNIAGFVNL LEVAKAANPQ PAIVWASSSS VYGLNTENPF
    260 270 280 290 300
    SEEHRTDQPA SLYAATKKAG EEIAHTYNHI YGLSLTGLRF FTVYGPWGRP
    310 320 330 340 350
    DMAYFFFTKD ILHGKSIDIY RTQDNQEVAR DFTYIDDIVK GCVGALDTAE
    360 370 380 390 400
    KSTGSGGKKR GQAQLRVYNL GNTSPVPVGR LVSILEGLLG TKAKKHLIKM
    410 420 430 440 450
    PRNGDVPYTH ANVSLAYKDF GYKPTTDLAA GLRKFVKWYV GYYGIQPRVK
    460
    KETSHAEDSA
    Length:460
    Mass (Da):50,570
    Last modified:October 1, 2000 - v1
    Checksum:i674B9F3E907DFF49
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ879893 Genomic DNA Translation: CAI53858.1
    AP001297 Genomic DNA Translation: BAB03000.1
    CP002686 Genomic DNA Translation: AEE76818.1
    AF083691 mRNA Translation: AAN60250.1
    AF370210 mRNA Translation: AAK44025.1
    AY056117 mRNA Translation: AAL07003.1
    AY062625 mRNA Translation: AAL32703.1
    AY133771 mRNA Translation: AAM91705.1
    RefSeqiNP_189024.1, NM_113287.3
    UniGeneiAt.24934
    At.67131
    At.71799

    Genome annotation databases

    EnsemblPlantsiAT3G23820.1; AT3G23820.1; AT3G23820
    GeneIDi821965
    GrameneiAT3G23820.1; AT3G23820.1; AT3G23820
    KEGGiath:AT3G23820

    Similar proteinsi

    Entry informationi

    Entry nameiGAE6_ARATH
    AccessioniPrimary (citable) accession number: Q9LIS3
    Secondary accession number(s): Q8H7G2
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
    Last sequence update: October 1, 2000
    Last modified: May 23, 2018
    This is version 122 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

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