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Protein

UDP-glucuronate 4-epimerase 6

Gene

GAE6

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the negatively charged monosaccharide that forms the backbone of pectic cell wall components.1 Publication

Catalytic activityi

UDP-glucuronate = UDP-D-galacturonate.

Kineticsi

No activity with UDP-Galactose, UDP- Glucose or UDP-Xylose.

  1. KM=0.23 mM for UDP-glucuronate1 Publication

    pH dependencei

    Optimum pH is 7.9. Active from pH 6 to 8.9.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei263Proton acceptorBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi113 – 144NADBy similarityAdd BLAST32

    GO - Molecular functioni

    GO - Biological processi

    • carbohydrate metabolic process Source: UniProtKB-KW
    • defense response to fungus Source: TAIR
    • defense response to Gram-negative bacterium Source: TAIR
    • galacturonate biosynthetic process Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BRENDAi5.1.3.6. 399.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucuronate 4-epimerase 6 (EC:5.1.3.6)
    Alternative name(s):
    UDP-glucuronic acid epimerase 6
    Short name:
    AtUGlcAE2
    Gene namesi
    Name:GAE6
    Synonyms:UGlcAE2
    Ordered Locus Names:At3g23820
    ORF Names:F14O13.1
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 3

    Organism-specific databases

    TAIRiAT3G23820.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei41 – 61HelicalSequence analysisAdd BLAST21
    Transmembranei111 – 131HelicalSequence analysisAdd BLAST21

    GO - Cellular componenti

    • endosome Source: TAIR
    • Golgi apparatus Source: TAIR
    • Golgi cisterna membrane Source: UniProtKB-SubCell
    • integral component of membrane Source: UniProtKB-KW
    • trans-Golgi network Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002926011 – 460UDP-glucuronate 4-epimerase 6Add BLAST460

    Proteomic databases

    PaxDbiQ9LIS3.

    PTM databases

    iPTMnetiQ9LIS3.

    Expressioni

    Tissue specificityi

    In roots, leaf veins, siliques, flowers, pollen and stems.3 Publications

    Gene expression databases

    ExpressionAtlasiQ9LIS3. baseline and differential.
    GenevisibleiQ9LIS3. AT.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CPK4Q388694EBI-2297116,EBI-979475

    Protein-protein interaction databases

    BioGridi7297. 1 interactor.
    IntActiQ9LIS3. 1 interactor.
    STRINGi3702.AT3G23820.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LIS3.
    SMRiQ9LIS3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi65 – 68Poly-Ala4
    Compositional biasi75 – 90Ser-richAdd BLAST16
    Compositional biasi237 – 240Poly-Ser4

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG410IU3F. Eukaryota.
    COG0451. LUCA.
    HOGENOMiHOG000168000.
    InParanoidiQ9LIS3.
    KOiK08679.
    OMAiNATQIIL.
    OrthoDBiEOG09360AZR.
    PhylomeDBiQ9LIS3.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR008089. Nuc_sugar_epim.
    [Graphical view]
    PfamiPF16363. GDP_Man_Dehyd. 1 hit.
    [Graphical view]
    PRINTSiPR01713. NUCEPIMERASE.
    SUPFAMiSSF51735. SSF51735. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9LIS3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPLSATADTS KTVKLERYNS YLRKIHSTKV LNASSKVLFR ATLLVALVLV
    60 70 80 90 100
    LIFAINYPPL SDSRAAAAHH LHRRSFLSTG LFSSSSSSSS IGGAAWEKRV
    110 120 130 140 150
    RQSSTAKRPH GLSVLVTGAA GFVGSHCSLA LRKRGDGVLG FDNFNDYYDP
    160 170 180 190 200
    SLKRARQELL EKQQVFIVEG DLNDGPLLRK LFDVVPFTHI LHLAAQAGVR
    210 220 230 240 250
    YAMKNPQSYI ASNIAGFVNL LEVAKAANPQ PAIVWASSSS VYGLNTENPF
    260 270 280 290 300
    SEEHRTDQPA SLYAATKKAG EEIAHTYNHI YGLSLTGLRF FTVYGPWGRP
    310 320 330 340 350
    DMAYFFFTKD ILHGKSIDIY RTQDNQEVAR DFTYIDDIVK GCVGALDTAE
    360 370 380 390 400
    KSTGSGGKKR GQAQLRVYNL GNTSPVPVGR LVSILEGLLG TKAKKHLIKM
    410 420 430 440 450
    PRNGDVPYTH ANVSLAYKDF GYKPTTDLAA GLRKFVKWYV GYYGIQPRVK
    460
    KETSHAEDSA
    Length:460
    Mass (Da):50,570
    Last modified:October 1, 2000 - v1
    Checksum:i674B9F3E907DFF49
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ879893 Genomic DNA. Translation: CAI53858.1.
    AP001297 Genomic DNA. Translation: BAB03000.1.
    CP002686 Genomic DNA. Translation: AEE76818.1.
    AF083691 mRNA. Translation: AAN60250.1.
    AF370210 mRNA. Translation: AAK44025.1.
    AY056117 mRNA. Translation: AAL07003.1.
    AY062625 mRNA. Translation: AAL32703.1.
    AY133771 mRNA. Translation: AAM91705.1.
    RefSeqiNP_189024.1. NM_113287.3.
    UniGeneiAt.24934.
    At.67131.
    At.71799.

    Genome annotation databases

    EnsemblPlantsiAT3G23820.1; AT3G23820.1; AT3G23820.
    GeneIDi821965.
    GrameneiAT3G23820.1; AT3G23820.1; AT3G23820.
    KEGGiath:AT3G23820.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ879893 Genomic DNA. Translation: CAI53858.1.
    AP001297 Genomic DNA. Translation: BAB03000.1.
    CP002686 Genomic DNA. Translation: AEE76818.1.
    AF083691 mRNA. Translation: AAN60250.1.
    AF370210 mRNA. Translation: AAK44025.1.
    AY056117 mRNA. Translation: AAL07003.1.
    AY062625 mRNA. Translation: AAL32703.1.
    AY133771 mRNA. Translation: AAM91705.1.
    RefSeqiNP_189024.1. NM_113287.3.
    UniGeneiAt.24934.
    At.67131.
    At.71799.

    3D structure databases

    ProteinModelPortaliQ9LIS3.
    SMRiQ9LIS3.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi7297. 1 interactor.
    IntActiQ9LIS3. 1 interactor.
    STRINGi3702.AT3G23820.1.

    PTM databases

    iPTMnetiQ9LIS3.

    Proteomic databases

    PaxDbiQ9LIS3.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT3G23820.1; AT3G23820.1; AT3G23820.
    GeneIDi821965.
    GrameneiAT3G23820.1; AT3G23820.1; AT3G23820.
    KEGGiath:AT3G23820.

    Organism-specific databases

    TAIRiAT3G23820.

    Phylogenomic databases

    eggNOGiENOG410IU3F. Eukaryota.
    COG0451. LUCA.
    HOGENOMiHOG000168000.
    InParanoidiQ9LIS3.
    KOiK08679.
    OMAiNATQIIL.
    OrthoDBiEOG09360AZR.
    PhylomeDBiQ9LIS3.

    Enzyme and pathway databases

    BRENDAi5.1.3.6. 399.

    Miscellaneous databases

    PROiQ9LIS3.

    Gene expression databases

    ExpressionAtlasiQ9LIS3. baseline and differential.
    GenevisibleiQ9LIS3. AT.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR016040. NAD(P)-bd_dom.
    IPR008089. Nuc_sugar_epim.
    [Graphical view]
    PfamiPF16363. GDP_Man_Dehyd. 1 hit.
    [Graphical view]
    PRINTSiPR01713. NUCEPIMERASE.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGAE6_ARATH
    AccessioniPrimary (citable) accession number: Q9LIS3
    Secondary accession number(s): Q8H7G2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 26, 2007
    Last sequence update: October 1, 2000
    Last modified: November 30, 2016
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.