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Protein

Beta-amylase 1, chloroplastic

Gene

BAM1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Beta-amylase activity. Can use p-nitrophenyl maltopentaoside (PNPG5) as substrate only in reduced form. Can play a minor role in the starch degradation and maltose metabolism in chloroplasts during the night. More active on phosphorylated glucan. Interacts directly with starch or other alpha-1,4-glucan.3 Publications

Catalytic activityi

Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.

Enzyme regulationi

Redox regulation; active in reducing conditions, inactive in oxidizing conditions. Thioredoxins f1, m1, and y1 mediate the reversible reductive activation of oxidized BAM1.1 Publication

pH dependencei

Optimum pH is 6-8.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei147SubstrateBy similarity1
Binding sitei187SubstrateBy similarity1
Binding sitei195SubstrateBy similarity1
Active sitei279Proton donorPROSITE-ProRule annotation1
Binding sitei392SubstrateBy similarity1
Binding sitei397SubstrateBy similarity1
Binding sitei439SubstrateBy similarity1
Active sitei477Proton acceptorPROSITE-ProRule annotation1
Binding sitei517SubstrateBy similarity1

GO - Molecular functioni

  • amylopectin maltohydrolase activity Source: UniProtKB-EC
  • beta-amylase activity Source: TAIR

GO - Biological processi

  • response to water deprivation Source: TAIR
  • starch catabolic process Source: TAIR

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation

Enzyme and pathway databases

BioCyciMetaCyc:AT3G23920-MONOMER
BRENDAi3.2.1.2 399

Protein family/group databases

CAZyiGH14 Glycoside Hydrolase Family 14

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-amylase 1, chloroplastic (EC:3.2.1.2)
Alternative name(s):
1,4-alpha-D-glucan maltohydrolase
Beta-amylase 7
Thioredoxin-regulated beta-amylase
Short name:
TR-BAMY
Gene namesi
Name:BAM1
Synonyms:BMY7, TRBAMY
Ordered Locus Names:At3g23920
ORF Names:F14O13.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G23920
TAIRilocus:2076086 AT3G23920

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Normal growth rate and starch breakdown in leaves during the night.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73C → S: Impaired redox inactivation under oxidizing conditions. 1 Publication1
Mutagenesisi189C → S: Normal redox inactivation under oxidizing conditions. 1 Publication1
Mutagenesisi247C → S: Normal redox inactivation under oxidizing conditions. 1 Publication1
Mutagenesisi302C → S: Normal redox inactivation under oxidizing conditions. 1 Publication1
Mutagenesisi440C → S: Normal redox inactivation under oxidizing conditions. 1 Publication1
Mutagenesisi454C → S: Normal redox inactivation under oxidizing conditions. 1 Publication1
Mutagenesisi511C → S: Impaired redox inactivation under oxidizing conditions. 1 Publication1
Mutagenesisi547C → S: Normal redox enzyme activation under oxidizing conditions. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 41Chloroplast1 PublicationAdd BLAST41
ChainiPRO_000039341642 – 575Beta-amylase 1, chloroplasticAdd BLAST534

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei55PhosphoserineCombined sources1
Modified residuei59PhosphoserineCombined sources1
Disulfide bondi73 ↔ 511Inhibitory under oxidizing conditions1 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ9LIR6
PRIDEiQ9LIR6

PTM databases

iPTMnetiQ9LIR6

Expressioni

Tissue specificityi

Expressed in leaves, roots, flowers, pollen, and seeds.1 Publication

Gene expression databases

ExpressionAtlasiQ9LIR6 baseline and differential
GenevisibleiQ9LIR6 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G23920.1

Structurei

3D structure databases

ProteinModelPortaliQ9LIR6
SMRiQ9LIR6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni478 – 479Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 14 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IE4H Eukaryota
ENOG410XRH5 LUCA
HOGENOMiHOG000238755
KOiK01177
OMAiMPISMNM
OrthoDBiEOG093605C7
PhylomeDBiQ9LIR6

Family and domain databases

InterProiView protein in InterPro
IPR001554 Glyco_hydro_14
IPR018238 Glyco_hydro_14_CS
IPR001371 Glyco_hydro_14B_pln
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR31352 PTHR31352, 1 hit
PfamiView protein in Pfam
PF01373 Glyco_hydro_14, 1 hit
PRINTSiPR00750 BETAAMYLASE
PR00842 GLHYDLASE14B
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00506 BETA_AMYLASE_1, 1 hit
PS00679 BETA_AMYLASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LIR6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALNLSHQLG VLAGTPIKSG EMTDSSLLSI SPPSARMMTP KAMNRNYKAH
60 70 80 90 100
GTDPSPPMSP ILGATRADLS VACKAFAVEN GIGTIEEQRT YREGGIGGKK
110 120 130 140 150
EGGGGVPVFV MMPLDSVTMG NTVNRRKAMK ASLQALKSAG VEGIMIDVWW
160 170 180 190 200
GLVEKESPGT YNWGGYNELL ELAKKLGLKV QAVMSFHQCG GNVGDSVTIP
210 220 230 240 250
LPQWVVEEVD KDPDLAYTDQ WGRRNHEYIS LGADTLPVLK GRTPVQCYAD
260 270 280 290 300
FMRAFRDNFK HLLGETIVEI QVGMGPAGEL RYPSYPEQEG TWKFPGIGAF
310 320 330 340 350
QCYDKYSLSS LKAAAETYGK PEWGSTGPTD AGHYNNWPED TQFFKKEGGG
360 370 380 390 400
WNSEYGDFFL SWYSQMLLDH GERILSSAKS IFENMGVKIS VKIAGIHWHY
410 420 430 440 450
GTRSHAPELT AGYYNTRFRD GYLPIAQMLA RHNAIFNFTC IEMRDHEQPQ
460 470 480 490 500
DALCAPEKLV NQVALATLAA EVPLAGENAL PRYDDYAHEQ ILKASALNLD
510 520 530 540 550
QNNEGEPREM CAFTYLRMNP ELFQADNWGK FVAFVKKMGE GRDSHRCREE
560 570
VEREAEHFVH VTQPLVQEAA VALTH
Length:575
Mass (Da):63,762
Last modified:October 1, 2000 - v1
Checksum:iCDCF1007217789A0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti405H → N in BAE98433 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001297 Genomic DNA Translation: BAB03009.1
CP002686 Genomic DNA Translation: AEE76832.1
AF367293 mRNA Translation: AAK56281.1
AY074393 mRNA Translation: AAL67089.1
AY078046 mRNA Translation: AAL77747.1
AY096517 mRNA Translation: AAM20167.1
AK226274 mRNA Translation: BAE98433.1
RefSeqiNP_189034.1, NM_113297.3
UniGeneiAt.8278

Genome annotation databases

EnsemblPlantsiAT3G23920.1; AT3G23920.1; AT3G23920
GeneIDi821975
GrameneiAT3G23920.1; AT3G23920.1; AT3G23920
KEGGiath:AT3G23920

Similar proteinsi

Entry informationi

Entry nameiBAM1_ARATH
AccessioniPrimary (citable) accession number: Q9LIR6
Secondary accession number(s): Q0WWR5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health