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Protein

Beta-amylase 1, chloroplastic

Gene

BAM1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Beta-amylase activity. Can use p-nitrophenyl maltopentaoside (PNPG5) as substrate only in reduced form. Can play a minor role in the starch degradation and maltose metabolism in chloroplasts during the night. More active on phosphorylated glucan. Interacts directly with starch or other alpha-1,4-glucan.3 Publications

Catalytic activityi

Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.

Enzyme regulationi

Redox regulation; active in reducing conditions, inactive in oxidizing conditions. Thioredoxins f1, m1, and y1 mediate the reversible reductive activation of oxidized BAM1.1 Publication

pH dependencei

Optimum pH is 6-8.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei147 – 1471SubstrateBy similarity
Binding sitei187 – 1871SubstrateBy similarity
Binding sitei195 – 1951SubstrateBy similarity
Active sitei279 – 2791Proton donorPROSITE-ProRule annotation
Binding sitei392 – 3921SubstrateBy similarity
Binding sitei397 – 3971SubstrateBy similarity
Binding sitei439 – 4391SubstrateBy similarity
Active sitei477 – 4771Proton acceptorPROSITE-ProRule annotation
Binding sitei517 – 5171SubstrateBy similarity

GO - Molecular functioni

  • beta-amylase activity Source: TAIR

GO - Biological processi

  • response to water deprivation Source: TAIR
  • starch catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Enzyme and pathway databases

BioCyciMetaCyc:AT3G23920-MONOMER.
BRENDAi3.2.1.2. 399.

Protein family/group databases

CAZyiGH14. Glycoside Hydrolase Family 14.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-amylase 1, chloroplastic (EC:3.2.1.2)
Alternative name(s):
1,4-alpha-D-glucan maltohydrolase
Beta-amylase 7
Thioredoxin-regulated beta-amylase
Short name:
TR-BAMY
Gene namesi
Name:BAM1
Synonyms:BMY7, TRBAMY
Ordered Locus Names:At3g23920
ORF Names:F14O13.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G23920.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: UniProtKB-SubCell
  • cytosol Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Normal growth rate and starch breakdown in leaves during the night.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi73 – 731C → S: Impaired redox inactivation under oxidizing conditions. 1 Publication
Mutagenesisi189 – 1891C → S: Normal redox inactivation under oxidizing conditions. 1 Publication
Mutagenesisi247 – 2471C → S: Normal redox inactivation under oxidizing conditions. 1 Publication
Mutagenesisi302 – 3021C → S: Normal redox inactivation under oxidizing conditions. 1 Publication
Mutagenesisi440 – 4401C → S: Normal redox inactivation under oxidizing conditions. 1 Publication
Mutagenesisi454 – 4541C → S: Normal redox inactivation under oxidizing conditions. 1 Publication
Mutagenesisi511 – 5111C → S: Impaired redox inactivation under oxidizing conditions. 1 Publication
Mutagenesisi547 – 5471C → S: Normal redox enzyme activation under oxidizing conditions. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4141Chloroplast1 PublicationAdd
BLAST
Chaini42 – 575534Beta-amylase 1, chloroplasticPRO_0000393416Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei55 – 551PhosphoserineCombined sources
Modified residuei59 – 591PhosphoserineCombined sources
Disulfide bondi73 ↔ 511Inhibitory under oxidizing conditions1 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ9LIR6.
PRIDEiQ9LIR6.

PTM databases

iPTMnetiQ9LIR6.

Expressioni

Tissue specificityi

Expressed in leaves, roots, flowers, pollen, and seeds.1 Publication

Gene expression databases

GenevisibleiQ9LIR6. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G23920.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LIR6.
SMRiQ9LIR6. Positions 103-538.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni478 – 4792Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 14 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IE4H. Eukaryota.
ENOG410XRH5. LUCA.
HOGENOMiHOG000238755.
KOiK01177.
OMAiFFKKEGG.
OrthoDBiEOG093605C7.
PhylomeDBiQ9LIR6.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001554. Glyco_hydro_14.
IPR018238. Glyco_hydro_14_CS.
IPR001371. Glyco_hydro_14B_pln.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01373. Glyco_hydro_14. 1 hit.
[Graphical view]
PRINTSiPR00750. BETAAMYLASE.
PR00842. GLHYDLASE14B.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00506. BETA_AMYLASE_1. 1 hit.
PS00679. BETA_AMYLASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LIR6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALNLSHQLG VLAGTPIKSG EMTDSSLLSI SPPSARMMTP KAMNRNYKAH
60 70 80 90 100
GTDPSPPMSP ILGATRADLS VACKAFAVEN GIGTIEEQRT YREGGIGGKK
110 120 130 140 150
EGGGGVPVFV MMPLDSVTMG NTVNRRKAMK ASLQALKSAG VEGIMIDVWW
160 170 180 190 200
GLVEKESPGT YNWGGYNELL ELAKKLGLKV QAVMSFHQCG GNVGDSVTIP
210 220 230 240 250
LPQWVVEEVD KDPDLAYTDQ WGRRNHEYIS LGADTLPVLK GRTPVQCYAD
260 270 280 290 300
FMRAFRDNFK HLLGETIVEI QVGMGPAGEL RYPSYPEQEG TWKFPGIGAF
310 320 330 340 350
QCYDKYSLSS LKAAAETYGK PEWGSTGPTD AGHYNNWPED TQFFKKEGGG
360 370 380 390 400
WNSEYGDFFL SWYSQMLLDH GERILSSAKS IFENMGVKIS VKIAGIHWHY
410 420 430 440 450
GTRSHAPELT AGYYNTRFRD GYLPIAQMLA RHNAIFNFTC IEMRDHEQPQ
460 470 480 490 500
DALCAPEKLV NQVALATLAA EVPLAGENAL PRYDDYAHEQ ILKASALNLD
510 520 530 540 550
QNNEGEPREM CAFTYLRMNP ELFQADNWGK FVAFVKKMGE GRDSHRCREE
560 570
VEREAEHFVH VTQPLVQEAA VALTH
Length:575
Mass (Da):63,762
Last modified:October 1, 2000 - v1
Checksum:iCDCF1007217789A0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti405 – 4051H → N in BAE98433 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001297 Genomic DNA. Translation: BAB03009.1.
CP002686 Genomic DNA. Translation: AEE76832.1.
AF367293 mRNA. Translation: AAK56281.1.
AY074393 mRNA. Translation: AAL67089.1.
AY078046 mRNA. Translation: AAL77747.1.
AY096517 mRNA. Translation: AAM20167.1.
AK226274 mRNA. Translation: BAE98433.1.
RefSeqiNP_189034.1. NM_113297.2.
UniGeneiAt.8278.

Genome annotation databases

EnsemblPlantsiAT3G23920.1; AT3G23920.1; AT3G23920.
GeneIDi821975.
GrameneiAT3G23920.1; AT3G23920.1; AT3G23920.
KEGGiath:AT3G23920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001297 Genomic DNA. Translation: BAB03009.1.
CP002686 Genomic DNA. Translation: AEE76832.1.
AF367293 mRNA. Translation: AAK56281.1.
AY074393 mRNA. Translation: AAL67089.1.
AY078046 mRNA. Translation: AAL77747.1.
AY096517 mRNA. Translation: AAM20167.1.
AK226274 mRNA. Translation: BAE98433.1.
RefSeqiNP_189034.1. NM_113297.2.
UniGeneiAt.8278.

3D structure databases

ProteinModelPortaliQ9LIR6.
SMRiQ9LIR6. Positions 103-538.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G23920.1.

Protein family/group databases

CAZyiGH14. Glycoside Hydrolase Family 14.

PTM databases

iPTMnetiQ9LIR6.

Proteomic databases

PaxDbiQ9LIR6.
PRIDEiQ9LIR6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G23920.1; AT3G23920.1; AT3G23920.
GeneIDi821975.
GrameneiAT3G23920.1; AT3G23920.1; AT3G23920.
KEGGiath:AT3G23920.

Organism-specific databases

TAIRiAT3G23920.

Phylogenomic databases

eggNOGiENOG410IE4H. Eukaryota.
ENOG410XRH5. LUCA.
HOGENOMiHOG000238755.
KOiK01177.
OMAiFFKKEGG.
OrthoDBiEOG093605C7.
PhylomeDBiQ9LIR6.

Enzyme and pathway databases

BioCyciMetaCyc:AT3G23920-MONOMER.
BRENDAi3.2.1.2. 399.

Miscellaneous databases

PROiQ9LIR6.

Gene expression databases

GenevisibleiQ9LIR6. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001554. Glyco_hydro_14.
IPR018238. Glyco_hydro_14_CS.
IPR001371. Glyco_hydro_14B_pln.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01373. Glyco_hydro_14. 1 hit.
[Graphical view]
PRINTSiPR00750. BETAAMYLASE.
PR00842. GLHYDLASE14B.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00506. BETA_AMYLASE_1. 1 hit.
PS00679. BETA_AMYLASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBAM1_ARATH
AccessioniPrimary (citable) accession number: Q9LIR6
Secondary accession number(s): Q0WWR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.