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Q9LIR6

- BAM1_ARATH

UniProt

Q9LIR6 - BAM1_ARATH

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Protein

Beta-amylase 1, chloroplastic

Gene

BAM1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Beta-amylase activity. Can use p-nitrophenyl maltopentaoside (PNPG5) as substrate only in reduced form. Can play a minor role in the starch degradation and maltose metabolism in chloroplasts during the night. More active on phosphorylated glucan. Interacts directly with starch or other alpha-1,4-glucan.3 Publications

Catalytic activityi

Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.

Enzyme regulationi

Redox regulation; active in reducing conditions, inactive in oxidizing conditions. Thioredoxins f1, m1, and y1 mediate the reversible reductive activation of oxidized BAM1.1 Publication

pH dependencei

Optimum pH is 6-8.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei147 – 1471SubstrateBy similarity
Binding sitei187 – 1871SubstrateBy similarity
Binding sitei195 – 1951SubstrateBy similarity
Active sitei279 – 2791Proton donorPROSITE-ProRule annotation
Binding sitei392 – 3921SubstrateBy similarity
Binding sitei397 – 3971SubstrateBy similarity
Binding sitei439 – 4391SubstrateBy similarity
Active sitei477 – 4771Proton acceptorPROSITE-ProRule annotation
Binding sitei517 – 5171SubstrateBy similarity

GO - Molecular functioni

  1. beta-amylase activity Source: TAIR

GO - Biological processi

  1. response to water deprivation Source: TAIR
  2. starch catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Enzyme and pathway databases

BioCyciMetaCyc:AT3G23920-MONOMER.
BRENDAi3.2.1.2. 399.

Protein family/group databases

CAZyiGH14. Glycoside Hydrolase Family 14.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-amylase 1, chloroplastic (EC:3.2.1.2)
Alternative name(s):
1,4-alpha-D-glucan maltohydrolase
Beta-amylase 7
Thioredoxin-regulated beta-amylase
Short name:
TR-BAMY
Gene namesi
Name:BAM1
Synonyms:BMY7, TRBAMY
Ordered Locus Names:At3g23920
ORF Names:F14O13.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G23920.

Subcellular locationi

Plastidchloroplast 2 Publications

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
  2. cytosol Source: TAIR
  3. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Normal growth rate and starch breakdown in leaves during the night.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi73 – 731C → S: Impaired redox inactivation under oxidizing conditions. 1 Publication
Mutagenesisi189 – 1891C → S: Normal redox inactivation under oxidizing conditions. 1 Publication
Mutagenesisi247 – 2471C → S: Normal redox inactivation under oxidizing conditions. 1 Publication
Mutagenesisi302 – 3021C → S: Normal redox inactivation under oxidizing conditions. 1 Publication
Mutagenesisi440 – 4401C → S: Normal redox inactivation under oxidizing conditions. 1 Publication
Mutagenesisi454 – 4541C → S: Normal redox inactivation under oxidizing conditions. 1 Publication
Mutagenesisi511 – 5111C → S: Impaired redox inactivation under oxidizing conditions. 1 Publication
Mutagenesisi547 – 5471C → S: Normal redox enzyme activation under oxidizing conditions. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4141Chloroplast1 PublicationAdd
BLAST
Chaini42 – 575534Beta-amylase 1, chloroplasticPRO_0000393416Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei55 – 551Phosphoserine1 Publication
Modified residuei59 – 591Phosphoserine1 Publication
Disulfide bondi73 ↔ 511Inhibitory under oxidizing conditions1 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ9LIR6.
PRIDEiQ9LIR6.

Expressioni

Tissue specificityi

Expressed in leaves, roots, flowers, pollen, and seeds.1 Publication

Gene expression databases

GenevestigatoriQ9LIR6.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G23920.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9LIR6.
SMRiQ9LIR6. Positions 103-538.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni478 – 4792Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 14 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiNOG77898.
HOGENOMiHOG000238755.
KOiK01177.
OMAiFMSWYSQ.
PhylomeDBiQ9LIR6.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001554. Glyco_hydro_14.
IPR018238. Glyco_hydro_14_CS.
IPR001371. Glyco_hydro_14B_pln.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01373. Glyco_hydro_14. 1 hit.
[Graphical view]
PRINTSiPR00750. BETAAMYLASE.
PR00842. GLHYDLASE14B.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00506. BETA_AMYLASE_1. 1 hit.
PS00679. BETA_AMYLASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LIR6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALNLSHQLG VLAGTPIKSG EMTDSSLLSI SPPSARMMTP KAMNRNYKAH
60 70 80 90 100
GTDPSPPMSP ILGATRADLS VACKAFAVEN GIGTIEEQRT YREGGIGGKK
110 120 130 140 150
EGGGGVPVFV MMPLDSVTMG NTVNRRKAMK ASLQALKSAG VEGIMIDVWW
160 170 180 190 200
GLVEKESPGT YNWGGYNELL ELAKKLGLKV QAVMSFHQCG GNVGDSVTIP
210 220 230 240 250
LPQWVVEEVD KDPDLAYTDQ WGRRNHEYIS LGADTLPVLK GRTPVQCYAD
260 270 280 290 300
FMRAFRDNFK HLLGETIVEI QVGMGPAGEL RYPSYPEQEG TWKFPGIGAF
310 320 330 340 350
QCYDKYSLSS LKAAAETYGK PEWGSTGPTD AGHYNNWPED TQFFKKEGGG
360 370 380 390 400
WNSEYGDFFL SWYSQMLLDH GERILSSAKS IFENMGVKIS VKIAGIHWHY
410 420 430 440 450
GTRSHAPELT AGYYNTRFRD GYLPIAQMLA RHNAIFNFTC IEMRDHEQPQ
460 470 480 490 500
DALCAPEKLV NQVALATLAA EVPLAGENAL PRYDDYAHEQ ILKASALNLD
510 520 530 540 550
QNNEGEPREM CAFTYLRMNP ELFQADNWGK FVAFVKKMGE GRDSHRCREE
560 570
VEREAEHFVH VTQPLVQEAA VALTH
Length:575
Mass (Da):63,762
Last modified:October 1, 2000 - v1
Checksum:iCDCF1007217789A0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti405 – 4051H → N in BAE98433. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001297 Genomic DNA. Translation: BAB03009.1.
CP002686 Genomic DNA. Translation: AEE76832.1.
AF367293 mRNA. Translation: AAK56281.1.
AY074393 mRNA. Translation: AAL67089.1.
AY078046 mRNA. Translation: AAL77747.1.
AY096517 mRNA. Translation: AAM20167.1.
AK226274 mRNA. Translation: BAE98433.1.
RefSeqiNP_189034.1. NM_113297.2.
UniGeneiAt.8278.

Genome annotation databases

EnsemblPlantsiAT3G23920.1; AT3G23920.1; AT3G23920.
GeneIDi821975.
KEGGiath:AT3G23920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001297 Genomic DNA. Translation: BAB03009.1 .
CP002686 Genomic DNA. Translation: AEE76832.1 .
AF367293 mRNA. Translation: AAK56281.1 .
AY074393 mRNA. Translation: AAL67089.1 .
AY078046 mRNA. Translation: AAL77747.1 .
AY096517 mRNA. Translation: AAM20167.1 .
AK226274 mRNA. Translation: BAE98433.1 .
RefSeqi NP_189034.1. NM_113297.2.
UniGenei At.8278.

3D structure databases

ProteinModelPortali Q9LIR6.
SMRi Q9LIR6. Positions 103-538.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT3G23920.1-P.

Protein family/group databases

CAZyi GH14. Glycoside Hydrolase Family 14.

Proteomic databases

PaxDbi Q9LIR6.
PRIDEi Q9LIR6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G23920.1 ; AT3G23920.1 ; AT3G23920 .
GeneIDi 821975.
KEGGi ath:AT3G23920.

Organism-specific databases

TAIRi AT3G23920.

Phylogenomic databases

eggNOGi NOG77898.
HOGENOMi HOG000238755.
KOi K01177.
OMAi FMSWYSQ.
PhylomeDBi Q9LIR6.

Enzyme and pathway databases

BioCyci MetaCyc:AT3G23920-MONOMER.
BRENDAi 3.2.1.2. 399.

Gene expression databases

Genevestigatori Q9LIR6.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001554. Glyco_hydro_14.
IPR018238. Glyco_hydro_14_CS.
IPR001371. Glyco_hydro_14B_pln.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF01373. Glyco_hydro_14. 1 hit.
[Graphical view ]
PRINTSi PR00750. BETAAMYLASE.
PR00842. GLHYDLASE14B.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00506. BETA_AMYLASE_1. 1 hit.
PS00679. BETA_AMYLASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 242-407.
    Strain: cv. Columbia.
  5. "Redox regulation of a novel plastid-targeted beta-amylase of Arabidopsis."
    Sparla F., Costa A., Lo Schiavo F., Pupillo P., Trost P.
    Plant Physiol. 141:840-850(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, DISULFIDE BOND, TRANSIT PEPTIDE CLEAVAGE SITE, MUTAGENESIS OF CYS-73; CYS-189; CYS-247; CYS-302; CYS-440; CYS-454; CYS-511 AND CYS-547.
  6. "Glucan, water dikinase activity stimulates breakdown of starch granules by plastidial beta-amylases."
    Edner C., Li J., Albrecht T., Mahlow S., Hejazi M., Hussain H., Kaplan F., Guy C., Smith S.M., Steup M., Ritte G.
    Plant Physiol. 145:17-28(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55 AND SER-59, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Root.
  8. "Beta-AMYLASE4, a noncatalytic protein required for starch breakdown, acts upstream of three active beta-amylases in Arabidopsis chloroplasts."
    Fulton D.C., Stettler M., Mettler T., Vaughan C.K., Li J., Francisco P., Gil M., Reinhold H., Eicke S., Messerli G., Dorken G., Halliday K., Smith A.M., Smith S.M., Zeeman S.C.
    Plant Cell 20:1040-1058(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, GENE FAMILY, NOMENCLATURE.
  9. "Catalytically-inactive beta-amylase BAM4 required for starch breakdown in Arabidopsis leaves is a starch-binding-protein."
    Li J., Francisco P., Zhou W., Edner C., Steup M., Ritte G., Bond C.S., Smith S.M.
    Arch. Biochem. Biophys. 489:92-98(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  10. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  11. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBAM1_ARATH
AccessioniPrimary (citable) accession number: Q9LIR6
Secondary accession number(s): Q0WWR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3