Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hydroxyethylthiazole kinase

Gene

THIM

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Thiazole kinase involved in thiamine salvage pathway.1 Publication

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.1 Publication

Cofactori

Mg2+By similarity

Kineticsi

kcat is 0.068 sec(-1) for thiazole. kcat is 0.069 sec(-1) for ATP.

  1. KM=30.5 µM for thiazole1 Publication
  2. KM=44.8 µM for ATP1 Publication

    Pathwayi: thiamine diphosphate biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.
    Proteins known to be involved in this subpathway in this organism are:
    1. Hydroxyethylthiazole kinase (THIM)
    This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei53 – 531Substrate; via amide nitrogenBy similarity
    Binding sitei202 – 2021Substrate; via amide nitrogenBy similarity

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • hydroxyethylthiazole kinase activity Source: UniProtKB
    • metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    • thiamine biosynthetic process Source: UniProtKB
    • thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
    • thiamine salvage Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G24030-MONOMER.
    UniPathwayiUPA00060; UER00139.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxyethylthiazole kinase (EC:2.7.1.50)
    Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinase
    Short name:
    TH kinase
    Short name:
    Thz kinase
    Gene namesi
    Name:THIM
    Ordered Locus Names:At3g24030
    ORF Names:F14O13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 3

    Organism-specific databases

    TAIRiAT3G24030.

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 276276Hydroxyethylthiazole kinasePRO_0000424279Add
    BLAST

    Proteomic databases

    PaxDbiQ9LIQ4.
    PRIDEiQ9LIQ4.

    PTM databases

    iPTMnetiQ9LIQ4.

    Expressioni

    Inductioni

    Not regulated by thiamine or 4-methyl-5-(2-phosphonooxyethyl)thiazole.1 Publication

    Gene expression databases

    GenevisibleiQ9LIQ4. AT.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT3G24030.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LIQ4.
    SMRiQ9LIQ4. Positions 18-266.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Thz kinase family.Curated

    Phylogenomic databases

    eggNOGiENOG410IDXU. Eukaryota.
    COG2145. LUCA.
    HOGENOMiHOG000114352.
    InParanoidiQ9LIQ4.
    KOiK00878.
    OMAiSPVMAHA.
    PhylomeDBiQ9LIQ4.

    Family and domain databases

    Gene3Di3.40.1190.20. 1 hit.
    HAMAPiMF_00228. Thz_kinase.
    InterProiIPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    [Graphical view]
    PfamiPF02110. HK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000513. Thz_kinase. 1 hit.
    PRINTSiPR01099. HYETHTZKNASE.
    SUPFAMiSSF53613. SSF53613. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9LIQ4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MESKSEQNEW SSGVWAHLTA VRQQSPLVQC ITNFVSMDLV ANTLLSAGAS
    60 70 80 90 100
    PAMVHSVVEI PDFTPHIHAL CVNVGTLTPD WLPSMKAAAE LASQLRKPWV
    110 120 130 140 150
    LDPAAVSCSG FRLKACLELI ELKPTVIKGN GSEIIALSSA SRGQTKGADS
    160 170 180 190 200
    SHESTDAIEA AKSLAMSSGA VVAVSGAVDI VTDGKQVIGV HNGTKMMQQI
    210 220 230 240 250
    TATGCSLAGL IVAFLAIDSS RVLEATVSAM AVFGIAGELG EAMANGPASL
    260 270
    RMHLIDCLYG LDETTVLKRV NVTRLG
    Length:276
    Mass (Da):28,795
    Last modified:October 1, 2000 - v1
    Checksum:i7A8B70E56E4942E0
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AP001297 Genomic DNA. Translation: BAB03021.1.
    CP002686 Genomic DNA. Translation: AEE76846.1.
    BT010426 mRNA. Translation: AAQ62427.1.
    AK175955 mRNA. Translation: BAD43718.1.
    RefSeqiNP_189045.1. NM_113308.5.
    UniGeneiAt.37606.
    At.37607.

    Genome annotation databases

    EnsemblPlantsiAT3G24030.1; AT3G24030.1; AT3G24030.
    GeneIDi821988.
    GrameneiAT3G24030.1; AT3G24030.1; AT3G24030.
    KEGGiath:AT3G24030.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AP001297 Genomic DNA. Translation: BAB03021.1.
    CP002686 Genomic DNA. Translation: AEE76846.1.
    BT010426 mRNA. Translation: AAQ62427.1.
    AK175955 mRNA. Translation: BAD43718.1.
    RefSeqiNP_189045.1. NM_113308.5.
    UniGeneiAt.37606.
    At.37607.

    3D structure databases

    ProteinModelPortaliQ9LIQ4.
    SMRiQ9LIQ4. Positions 18-266.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT3G24030.1.

    PTM databases

    iPTMnetiQ9LIQ4.

    Proteomic databases

    PaxDbiQ9LIQ4.
    PRIDEiQ9LIQ4.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT3G24030.1; AT3G24030.1; AT3G24030.
    GeneIDi821988.
    GrameneiAT3G24030.1; AT3G24030.1; AT3G24030.
    KEGGiath:AT3G24030.

    Organism-specific databases

    TAIRiAT3G24030.

    Phylogenomic databases

    eggNOGiENOG410IDXU. Eukaryota.
    COG2145. LUCA.
    HOGENOMiHOG000114352.
    InParanoidiQ9LIQ4.
    KOiK00878.
    OMAiSPVMAHA.
    PhylomeDBiQ9LIQ4.

    Enzyme and pathway databases

    UniPathwayiUPA00060; UER00139.
    BioCyciARA:AT3G24030-MONOMER.

    Miscellaneous databases

    PROiQ9LIQ4.

    Gene expression databases

    GenevisibleiQ9LIQ4. AT.

    Family and domain databases

    Gene3Di3.40.1190.20. 1 hit.
    HAMAPiMF_00228. Thz_kinase.
    InterProiIPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    [Graphical view]
    PfamiPF02110. HK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000513. Thz_kinase. 1 hit.
    PRINTSiPR01099. HYETHTZKNASE.
    SUPFAMiSSF53613. SSF53613. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
      DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Identification of the thiamin salvage enzyme thiazole kinase in Arabidopsis and maize."
      Yazdani M., Zallot R., Tunc-Ozdemir M., de Crecy-Lagard V., Shintani D.K., Hanson A.D.
      Phytochemistry 94:68-73(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, INDUCTION BY THIAMINE AND 4-METHYL-5-(2-PHOSPHONOOXYETHYL)THIAZOLE.

    Entry informationi

    Entry nameiTHIM_ARATH
    AccessioniPrimary (citable) accession number: Q9LIQ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2013
    Last sequence update: October 1, 2000
    Last modified: February 17, 2016
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.