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Protein

Hydroxyethylthiazole kinase

Gene

THIM

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thiazole kinase involved in thiamine salvage pathway.1 Publication

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.1 Publication

Cofactori

Mg2+By similarity

Kineticsi

kcat is 0.068 sec(-1) for thiazole. kcat is 0.069 sec(-1) for ATP.
  1. KM=30.5 µM for thiazole1 Publication
  2. KM=44.8 µM for ATP1 Publication

    Pathwayi: thiamine diphosphate biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.
    Proteins known to be involved in this subpathway in this organism are:
    1. Hydroxyethylthiazole kinase (THIM)
    This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei53Substrate; via amide nitrogenBy similarity1
    Binding sitei202Substrate; via amide nitrogenBy similarity1

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • hydroxyethylthiazole kinase activity Source: UniProtKB
    • metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    • thiamine biosynthetic process Source: UniProtKB
    • thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
    • thiamine salvage Source: UniProtKB

    Keywordsi

    Molecular functionKinase, Transferase
    Biological processThiamine biosynthesis
    LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G24030-MONOMER
    UniPathwayiUPA00060; UER00139

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxyethylthiazole kinase (EC:2.7.1.50)
    Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinase
    Short name:
    TH kinase
    Short name:
    Thz kinase
    Gene namesi
    Name:THIM
    Ordered Locus Names:At3g24030
    ORF Names:F14O13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 3

    Organism-specific databases

    AraportiAT3G24030
    TAIRilocus:2076171 AT3G24030

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004242791 – 276Hydroxyethylthiazole kinaseAdd BLAST276

    Proteomic databases

    PaxDbiQ9LIQ4

    Expressioni

    Inductioni

    Not regulated by thiamine or 4-methyl-5-(2-phosphonooxyethyl)thiazole.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9LIQ4 baseline and differential
    GenevisibleiQ9LIQ4 AT

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT3G24030.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LIQ4
    SMRiQ9LIQ4
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Thz kinase family.Curated

    Phylogenomic databases

    eggNOGiENOG410IDXU Eukaryota
    COG2145 LUCA
    HOGENOMiHOG000114352
    InParanoidiQ9LIQ4
    KOiK00878
    OMAiKRPLVHN
    OrthoDBiEOG09360LV6
    PhylomeDBiQ9LIQ4

    Family and domain databases

    CDDicd01170 THZ_kinase, 1 hit
    Gene3Di3.40.1190.20, 1 hit
    HAMAPiMF_00228 Thz_kinase, 1 hit
    InterProiView protein in InterPro
    IPR000417 Hyethyz_kinase
    IPR029056 Ribokinase-like
    PANTHERiPTHR44423 PTHR44423, 1 hit
    PfamiView protein in Pfam
    PF02110 HK, 1 hit
    PIRSFiPIRSF000513 Thz_kinase, 1 hit
    PRINTSiPR01099 HYETHTZKNASE
    SUPFAMiSSF53613 SSF53613, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9LIQ4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MESKSEQNEW SSGVWAHLTA VRQQSPLVQC ITNFVSMDLV ANTLLSAGAS
    60 70 80 90 100
    PAMVHSVVEI PDFTPHIHAL CVNVGTLTPD WLPSMKAAAE LASQLRKPWV
    110 120 130 140 150
    LDPAAVSCSG FRLKACLELI ELKPTVIKGN GSEIIALSSA SRGQTKGADS
    160 170 180 190 200
    SHESTDAIEA AKSLAMSSGA VVAVSGAVDI VTDGKQVIGV HNGTKMMQQI
    210 220 230 240 250
    TATGCSLAGL IVAFLAIDSS RVLEATVSAM AVFGIAGELG EAMANGPASL
    260 270
    RMHLIDCLYG LDETTVLKRV NVTRLG
    Length:276
    Mass (Da):28,795
    Last modified:October 1, 2000 - v1
    Checksum:i7A8B70E56E4942E0
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AP001297 Genomic DNA Translation: BAB03021.1
    CP002686 Genomic DNA Translation: AEE76846.1
    BT010426 mRNA Translation: AAQ62427.1
    AK175955 mRNA Translation: BAD43718.1
    RefSeqiNP_001319628.1, NM_001338665.1
    UniGeneiAt.37606
    At.37607

    Genome annotation databases

    EnsemblPlantsiAT3G24030.1; AT3G24030.1; AT3G24030
    GeneIDi821988
    GrameneiAT3G24030.1; AT3G24030.1; AT3G24030
    KEGGiath:AT3G24030

    Similar proteinsi

    Entry informationi

    Entry nameiTHIM_ARATH
    AccessioniPrimary (citable) accession number: Q9LIQ4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2013
    Last sequence update: October 1, 2000
    Last modified: April 25, 2018
    This is version 114 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health