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Q9LIP2

- PSB5B_ARATH

UniProt

Q9LIP2 - PSB5B_ARATH

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Protein

Proteasome subunit beta type-5-B

Gene

PBE2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei58 – 581NucleophileBy similarity

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

BioCyciARA:AT3G26340-MONOMER.
ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_107429. Orc1 removal from chromatin.
REACT_185297. Separation of Sister Chromatids.
REACT_187712. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_187719. ER-Phagosome pathway.
REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_190947. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

Protein family/group databases

MEROPSiT01.A10.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-5-B (EC:3.4.25.1)
Alternative name(s):
20S proteasome beta subunit E-2
Proteasome epsilon-2 chain
Gene namesi
Name:PBE2
Ordered Locus Names:At3g26340
ORF Names:F20C19.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G26340.

Subcellular locationi

Cytoplasm PROSITE-ProRule annotation. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
  3. proteasome complex Source: TAIR
  4. proteasome core complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 5757Removed in mature formBy similarityPRO_0000042826Add
BLAST
Chaini58 – 273216Proteasome subunit beta type-5-BPRO_0000042827Add
BLAST

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiQ9LIP2.
PRIDEiQ9LIP2.

Expressioni

Gene expression databases

GenevestigatoriQ9LIP2.

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.2 Publications

Protein-protein interaction databases

BioGridi7569. 3 interactions.
IntActiQ9LIP2. 2 interactions.
STRINGi3702.AT3G26340.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9LIP2.
SMRiQ9LIP2. Positions 17-256.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000091082.
InParanoidiQ9LIP2.
KOiK02737.
OMAiTMCAGVT.
PhylomeDBiQ9LIP2.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LIP2 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLDTSGLET TMPVIGFGSN SEMLDGFSSA PSFDLPRTTD FDGFQKKAVE
60 70 80 90 100
MVKPAKGTTT LAFIFKEGVM VAADSRASMG GYISSQSVKK IIEINPYMLG
110 120 130 140 150
TMAGGAADCQ FWHRNLGIKC RLHELANKRR ISVSGASKLL ANMLYSYRGM
160 170 180 190 200
GLSVGTMIAG WDETGPGLYY VDNEGGRLKG DRFSVGSGSP YAYGVLDSGY
210 220 230 240 250
KFDMSVEEAS ELARRSIYHA TFRDGASGGV ASVYHVGPQG WTKLSGDDVG
260 270
ELHYHYYPVA PITAEHVMEE AAE
Length:273
Mass (Da):29,485
Last modified:October 1, 2000 - v1
Checksum:iFAE197B491BDE6A7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP001298 Genomic DNA. Translation: BAB02194.1.
CP002686 Genomic DNA. Translation: AEE77147.1.
AY125569 mRNA. Translation: AAM78079.1.
AF439846 mRNA. Translation: AAL27514.1.
AY085678 mRNA. Translation: AAM62897.1.
RefSeqiNP_189265.1. NM_113541.3.
UniGeneiAt.6729.

Genome annotation databases

EnsemblPlantsiAT3G26340.1; AT3G26340.1; AT3G26340.
GeneIDi822238.
KEGGiath:AT3G26340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP001298 Genomic DNA. Translation: BAB02194.1 .
CP002686 Genomic DNA. Translation: AEE77147.1 .
AY125569 mRNA. Translation: AAM78079.1 .
AF439846 mRNA. Translation: AAL27514.1 .
AY085678 mRNA. Translation: AAM62897.1 .
RefSeqi NP_189265.1. NM_113541.3.
UniGenei At.6729.

3D structure databases

ProteinModelPortali Q9LIP2.
SMRi Q9LIP2. Positions 17-256.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 7569. 3 interactions.
IntActi Q9LIP2. 2 interactions.
STRINGi 3702.AT3G26340.1-P.

Protein family/group databases

MEROPSi T01.A10.

Proteomic databases

PaxDbi Q9LIP2.
PRIDEi Q9LIP2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G26340.1 ; AT3G26340.1 ; AT3G26340 .
GeneIDi 822238.
KEGGi ath:AT3G26340.

Organism-specific databases

TAIRi AT3G26340.

Phylogenomic databases

eggNOGi COG0638.
HOGENOMi HOG000091082.
InParanoidi Q9LIP2.
KOi K02737.
OMAi TMCAGVT.
PhylomeDBi Q9LIP2.

Enzyme and pathway databases

BioCyci ARA:AT3G26340-MONOMER.
Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_107429. Orc1 removal from chromatin.
REACT_185297. Separation of Sister Chromatids.
REACT_187712. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_187719. ER-Phagosome pathway.
REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_190947. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

Gene expression databases

Genevestigatori Q9LIP2.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
InterProi IPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
[Graphical view ]
PRINTSi PR00141. PROTEASOME.
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms."
    Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D.
    J. Biol. Chem. 279:6401-6413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
  6. "Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse array of plant proteolytic complexes."
    Book A.J., Gladman N.P., Lee S.S., Scalf M., Smith L.M., Vierstra R.D.
    J. Biol. Chem. 285:25554-25569(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, CHARACTERIZATION OF THE 26S PROTEASOME COMPLEX, SUBUNIT.

Entry informationi

Entry nameiPSB5B_ARATH
AccessioniPrimary (citable) accession number: Q9LIP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3