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Protein

Putative calcium-transporting ATPase 13, plasma membrane-type

Gene

ACA13

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles.By similarity

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Enzyme regulationi

Activated by calmodulin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4494-aspartylphosphate intermediateBy similarity1
Metal bindingi747MagnesiumBy similarity1
Metal bindingi751MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-transporting ATPase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G22910-MONOMER.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative calcium-transporting ATPase 13, plasma membrane-type (EC:3.6.3.8)
Alternative name(s):
Ca(2+)-ATPase isoform 13
Gene namesi
Name:ACA13
Ordered Locus Names:At3g22910
ORF Names:F5N5.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G22910.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 147CytoplasmicSequence analysisAdd BLAST147
Transmembranei148 – 168HelicalSequence analysisAdd BLAST21
Topological domaini169 – 186LumenalSequence analysisAdd BLAST18
Transmembranei187 – 207HelicalSequence analysisAdd BLAST21
Topological domaini208 – 336CytoplasmicSequence analysisAdd BLAST129
Transmembranei337 – 356HelicalSequence analysisAdd BLAST20
Topological domaini357 – 393LumenalSequence analysisAdd BLAST37
Transmembranei394 – 411HelicalSequence analysisAdd BLAST18
Topological domaini412 – 802CytoplasmicSequence analysisAdd BLAST391
Transmembranei803 – 821HelicalSequence analysisAdd BLAST19
Topological domaini822 – 832LumenalSequence analysisAdd BLAST11
Transmembranei833 – 853HelicalSequence analysisAdd BLAST21
Topological domaini854 – 873CytoplasmicSequence analysisAdd BLAST20
Transmembranei874 – 896HelicalSequence analysisAdd BLAST23
Topological domaini897 – 905LumenalSequence analysis9
Transmembranei906 – 926HelicalSequence analysisAdd BLAST21
Topological domaini927 – 944CytoplasmicSequence analysisAdd BLAST18
Transmembranei945 – 966HelicalSequence analysisAdd BLAST22
Topological domaini967 – 976LumenalSequence analysis10
Transmembranei977 – 998HelicalSequence analysisAdd BLAST22
Topological domaini999 – 1002CytoplasmicSequence analysis4

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464191 – 1017Putative calcium-transporting ATPase 13, plasma membrane-typeAdd BLAST1017

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9LIK7.
PRIDEiQ9LIK7.

PTM databases

iPTMnetiQ9LIK7.

Expressioni

Gene expression databases

GenevisibleiQ9LIK7. AT.

Interactioni

Protein-protein interaction databases

BioGridi7195. 1 interactor.
MINTiMINT-8060707.
STRINGi3702.AT3G22910.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LIK7.
SMRiQ9LIK7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 31Interaction with calmodulinCuratedAdd BLAST12

Domaini

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
HOGENOMiHOG000265623.
InParanoidiQ9LIK7.
KOiK01537.
OMAiAYSEDNE.
OrthoDBiEOG093601Z7.
PhylomeDBiQ9LIK7.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LIK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRNVSDHAE KKDKVGVEVL LELPKTLSKS NKKWQLALIK LYCSRTLLNC
60 70 80 90 100
AKHAIRKPGL FPRSLSYTAI DLDHHHGDDH FKIDTETLND LVKNKNQEKL
110 120 130 140 150
ESLGGPNGLV SALKSNTRLG INEEGDEIQR RRSTFGSNTY TRQPSKGLFH
160 170 180 190 200
FVVEAFKDLT ILILLGCATL SLGFGIKEHG LKEGWYDGGS IFVAVFLVVA
210 220 230 240 250
VSAVSNFRQN RQFDKLSKVS SNIKIDVVRN GRRQEISIFD IVVGDIVCLN
260 270 280 290 300
IGDQVPADGV FVEGHLLHVD ESSMTGESDH VEVSLTGNTF LFSGTKIADG
310 320 330 340 350
FGKMAVTSVG MNTAWGQMMS HISRDTNEQT PLQSRLDKLT SSIGKVGLLV
360 370 380 390 400
AFLVLLVLLI RYFTGTTKDE SGNREYNGKT TKSDEIVNAV VKMVAAAVTI
410 420 430 440 450
IVVAIPEGLP LAVTLTLAYS MKRMMKDNAM VRKLSACETM GSATVICTDK
460 470 480 490 500
TGTLTLNQMK VTDFWFGLES GKASSVSQRV VELFHQGVAM NTTGSVFKAK
510 520 530 540 550
AGTEYEFSGS PTEKAILSWA VEELEMGMEK VIEEHDVVHV EGFNSEKKRS
560 570 580 590 600
GVLMKKKGVN TENNVVHWKG AAEKILAMCS TFCDGSGVVR EMKEDDKIQF
610 620 630 640 650
EKIIQSMAAK SLRCIAFAYS EDNEDNKKLK EEKLSLLGII GIKDPCRPGV
660 670 680 690 700
KKAVEDCQFA GVNIKMITGD NIFTARAIAV ECGILTPEDE MNSEAVLEGE
710 720 730 740 750
KFRNYTQEER LEKVERIKVM ARSSPFDKLL MVKCLKELGH VVAVTGDGTN
760 770 780 790 800
DAPALKEADI GLSMGIQGTE VAKESSDIVI LDDNFASVAT VLKWGRCVYN
810 820 830 840 850
NIQKFIQFQL TVNVAALVIN FVAAVSAGDV PLTAVQLLWV NLIMDTLGAL
860 870 880 890 900
ALATEKPTND LMKKKPIGRV APLITNIMWR NLLAQAFYQI SVLLVLQFRG
910 920 930 940 950
RSIFNVTEKV KNTLIFNTFV LCQVFNEFNA RSLEKKNVFK GLHKNRLFIG
960 970 980 990 1000
IIVVTVVLQV VMVEFLKRFA DTERLNLGQW GVCIAIAAAS WPIGWLVKSV
1010
PVPERHFFSY LKWKKRS
Length:1,017
Mass (Da):112,528
Last modified:October 1, 2000 - v1
Checksum:i2F0265CCE8862916
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001300 Genomic DNA. Translation: BAB03036.1.
CP002686 Genomic DNA. Translation: AEE76690.1.
RefSeqiNP_188931.1. NM_113191.2.
UniGeneiAt.65128.

Genome annotation databases

EnsemblPlantsiAT3G22910.1; AT3G22910.1; AT3G22910.
GeneIDi821863.
GrameneiAT3G22910.1; AT3G22910.1; AT3G22910.
KEGGiath:AT3G22910.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001300 Genomic DNA. Translation: BAB03036.1.
CP002686 Genomic DNA. Translation: AEE76690.1.
RefSeqiNP_188931.1. NM_113191.2.
UniGeneiAt.65128.

3D structure databases

ProteinModelPortaliQ9LIK7.
SMRiQ9LIK7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7195. 1 interactor.
MINTiMINT-8060707.
STRINGi3702.AT3G22910.1.

PTM databases

iPTMnetiQ9LIK7.

Proteomic databases

PaxDbiQ9LIK7.
PRIDEiQ9LIK7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G22910.1; AT3G22910.1; AT3G22910.
GeneIDi821863.
GrameneiAT3G22910.1; AT3G22910.1; AT3G22910.
KEGGiath:AT3G22910.

Organism-specific databases

TAIRiAT3G22910.

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
HOGENOMiHOG000265623.
InParanoidiQ9LIK7.
KOiK01537.
OMAiAYSEDNE.
OrthoDBiEOG093601Z7.
PhylomeDBiQ9LIK7.

Enzyme and pathway databases

BioCyciARA:AT3G22910-MONOMER.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiQ9LIK7.

Gene expression databases

GenevisibleiQ9LIK7. AT.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACA13_ARATH
AccessioniPrimary (citable) accession number: Q9LIK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.