Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative calcium-transporting ATPase 13, plasma membrane-type

Gene

ACA13

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles.By similarity

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Enzyme regulationi

Activated by calmodulin.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei449 – 44914-aspartylphosphate intermediateBy similarity
Metal bindingi747 – 7471MagnesiumBy similarity
Metal bindingi751 – 7511MagnesiumBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G22910-MONOMER.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative calcium-transporting ATPase 13, plasma membrane-type (EC:3.6.3.8)
Alternative name(s):
Ca(2+)-ATPase isoform 13
Gene namesi
Name:ACA13
Ordered Locus Names:At3g22910
ORF Names:F5N5.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G22910.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 147147CytoplasmicSequence analysisAdd
BLAST
Transmembranei148 – 16821HelicalSequence analysisAdd
BLAST
Topological domaini169 – 18618LumenalSequence analysisAdd
BLAST
Transmembranei187 – 20721HelicalSequence analysisAdd
BLAST
Topological domaini208 – 336129CytoplasmicSequence analysisAdd
BLAST
Transmembranei337 – 35620HelicalSequence analysisAdd
BLAST
Topological domaini357 – 39337LumenalSequence analysisAdd
BLAST
Transmembranei394 – 41118HelicalSequence analysisAdd
BLAST
Topological domaini412 – 802391CytoplasmicSequence analysisAdd
BLAST
Transmembranei803 – 82119HelicalSequence analysisAdd
BLAST
Topological domaini822 – 83211LumenalSequence analysisAdd
BLAST
Transmembranei833 – 85321HelicalSequence analysisAdd
BLAST
Topological domaini854 – 87320CytoplasmicSequence analysisAdd
BLAST
Transmembranei874 – 89623HelicalSequence analysisAdd
BLAST
Topological domaini897 – 9059LumenalSequence analysis
Transmembranei906 – 92621HelicalSequence analysisAdd
BLAST
Topological domaini927 – 94418CytoplasmicSequence analysisAdd
BLAST
Transmembranei945 – 96622HelicalSequence analysisAdd
BLAST
Topological domaini967 – 97610LumenalSequence analysis
Transmembranei977 – 99822HelicalSequence analysisAdd
BLAST
Topological domaini999 – 10024CytoplasmicSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10171017Putative calcium-transporting ATPase 13, plasma membrane-typePRO_0000046419Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9LIK7.
PRIDEiQ9LIK7.

PTM databases

iPTMnetiQ9LIK7.

Expressioni

Gene expression databases

GenevisibleiQ9LIK7. AT.

Interactioni

Protein-protein interaction databases

BioGridi7195. 1 interaction.
MINTiMINT-8060707.
STRINGi3702.AT3G22910.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LIK7.
SMRiQ9LIK7. Positions 126-888.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni20 – 3112Interaction with calmodulinCuratedAdd
BLAST

Domaini

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
HOGENOMiHOG000265623.
InParanoidiQ9LIK7.
KOiK01537.
OMAiAYSEDNE.
PhylomeDBiQ9LIK7.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LIK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRNVSDHAE KKDKVGVEVL LELPKTLSKS NKKWQLALIK LYCSRTLLNC
60 70 80 90 100
AKHAIRKPGL FPRSLSYTAI DLDHHHGDDH FKIDTETLND LVKNKNQEKL
110 120 130 140 150
ESLGGPNGLV SALKSNTRLG INEEGDEIQR RRSTFGSNTY TRQPSKGLFH
160 170 180 190 200
FVVEAFKDLT ILILLGCATL SLGFGIKEHG LKEGWYDGGS IFVAVFLVVA
210 220 230 240 250
VSAVSNFRQN RQFDKLSKVS SNIKIDVVRN GRRQEISIFD IVVGDIVCLN
260 270 280 290 300
IGDQVPADGV FVEGHLLHVD ESSMTGESDH VEVSLTGNTF LFSGTKIADG
310 320 330 340 350
FGKMAVTSVG MNTAWGQMMS HISRDTNEQT PLQSRLDKLT SSIGKVGLLV
360 370 380 390 400
AFLVLLVLLI RYFTGTTKDE SGNREYNGKT TKSDEIVNAV VKMVAAAVTI
410 420 430 440 450
IVVAIPEGLP LAVTLTLAYS MKRMMKDNAM VRKLSACETM GSATVICTDK
460 470 480 490 500
TGTLTLNQMK VTDFWFGLES GKASSVSQRV VELFHQGVAM NTTGSVFKAK
510 520 530 540 550
AGTEYEFSGS PTEKAILSWA VEELEMGMEK VIEEHDVVHV EGFNSEKKRS
560 570 580 590 600
GVLMKKKGVN TENNVVHWKG AAEKILAMCS TFCDGSGVVR EMKEDDKIQF
610 620 630 640 650
EKIIQSMAAK SLRCIAFAYS EDNEDNKKLK EEKLSLLGII GIKDPCRPGV
660 670 680 690 700
KKAVEDCQFA GVNIKMITGD NIFTARAIAV ECGILTPEDE MNSEAVLEGE
710 720 730 740 750
KFRNYTQEER LEKVERIKVM ARSSPFDKLL MVKCLKELGH VVAVTGDGTN
760 770 780 790 800
DAPALKEADI GLSMGIQGTE VAKESSDIVI LDDNFASVAT VLKWGRCVYN
810 820 830 840 850
NIQKFIQFQL TVNVAALVIN FVAAVSAGDV PLTAVQLLWV NLIMDTLGAL
860 870 880 890 900
ALATEKPTND LMKKKPIGRV APLITNIMWR NLLAQAFYQI SVLLVLQFRG
910 920 930 940 950
RSIFNVTEKV KNTLIFNTFV LCQVFNEFNA RSLEKKNVFK GLHKNRLFIG
960 970 980 990 1000
IIVVTVVLQV VMVEFLKRFA DTERLNLGQW GVCIAIAAAS WPIGWLVKSV
1010
PVPERHFFSY LKWKKRS
Length:1,017
Mass (Da):112,528
Last modified:October 1, 2000 - v1
Checksum:i2F0265CCE8862916
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001300 Genomic DNA. Translation: BAB03036.1.
CP002686 Genomic DNA. Translation: AEE76690.1.
RefSeqiNP_188931.1. NM_113191.1.
UniGeneiAt.65128.

Genome annotation databases

EnsemblPlantsiAT3G22910.1; AT3G22910.1; AT3G22910.
GeneIDi821863.
GrameneiAT3G22910.1; AT3G22910.1; AT3G22910.
KEGGiath:AT3G22910.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001300 Genomic DNA. Translation: BAB03036.1.
CP002686 Genomic DNA. Translation: AEE76690.1.
RefSeqiNP_188931.1. NM_113191.1.
UniGeneiAt.65128.

3D structure databases

ProteinModelPortaliQ9LIK7.
SMRiQ9LIK7. Positions 126-888.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7195. 1 interaction.
MINTiMINT-8060707.
STRINGi3702.AT3G22910.1.

PTM databases

iPTMnetiQ9LIK7.

Proteomic databases

PaxDbiQ9LIK7.
PRIDEiQ9LIK7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G22910.1; AT3G22910.1; AT3G22910.
GeneIDi821863.
GrameneiAT3G22910.1; AT3G22910.1; AT3G22910.
KEGGiath:AT3G22910.

Organism-specific databases

TAIRiAT3G22910.

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
HOGENOMiHOG000265623.
InParanoidiQ9LIK7.
KOiK01537.
OMAiAYSEDNE.
PhylomeDBiQ9LIK7.

Enzyme and pathway databases

BioCyciARA:AT3G22910-MONOMER.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiQ9LIK7.

Gene expression databases

GenevisibleiQ9LIK7. AT.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiACA13_ARATH
AccessioniPrimary (citable) accession number: Q9LIK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: April 13, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.