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Q9LIK0 (PKP1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Plastidial pyruvate kinase 1, chloroplastic

Short name=PK1
Short name=PKp1
EC=2.7.1.40
Alternative name(s):
Pyruvate kinase II
Pyruvate kinase isozyme A
Short name=PKP-ALPHA
Gene names
Name:PKP1
Synonyms:PK1
Ordered Locus Names:At3g22960
ORF Names:F5N5.15
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length596 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for plastidial pyruvate kinase activity. Involved in seed oil accumulation, embryo development and seed storage compounds mobilization upon germination. Ref.9 Ref.10

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium. Ref.8

Potassium. Ref.8

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Oligomer of alpha and beta subunits. Ref.8

Subcellular location

Plastidchloroplast stroma Ref.5 Ref.6 Ref.8 Ref.9 Ref.11 Ref.12 Ref.13.

Tissue specificity

Mostly expressed in seeds, and, to a lower extent, in roots, leaves (veins and trichomes), stems, inflorescences, siliques, pollen (grains and tubes) and flowers (sepals and petals). Ref.8 Ref.9

Developmental stage

In seeds, accumulates in endosperm and embryo. In torpedo-shaped embryos, restricted to the hypocotyl and in the outer parts of the young cotyledons. In later embryo stages, present in all tissues except root tips. Ref.9

Induction

Induced by cadmium.

Disruption phenotype

Reduced plastidial pyruvate kinase activity and altered seed oil content leading to wrinkled seeds, retarded embryo elongation and reduced seed germination. Ref.9 Ref.10

Sequence similarities

Belongs to the pyruvate kinase family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.8-8.2. Ref.8 Ref.9

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 8787Chloroplast By similarity
Chain88 – 596509Plastidial pyruvate kinase 1, chloroplastic
PRO_0000416987

Sites

Metal binding1491Potassium By similarity
Metal binding1811Potassium By similarity
Metal binding1821Potassium; via carbonyl oxygen By similarity
Metal binding3461Magnesium Potential
Metal binding3701Magnesium By similarity
Binding site1471Substrate By similarity
Binding site3691Substrate; via amide nitrogen By similarity
Binding site3701Substrate; via amide nitrogen By similarity
Binding site4021Substrate By similarity
Site3441Transition state stabilizer By similarity

Experimental info

Sequence conflict91T → S in AAM61702. Ref.4
Sequence conflict521S → P in AAM61702. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9LIK0 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 256CE329639A4BFD

FASTA59665,131
        10         20         30         40         50         60 
MSQSIQFSTP SHTPHLLHLP HSQFNRPLSS ISFRRFPLTT IKYTSIRASS SSSPSPDLDS 

        70         80         90        100        110        120 
SSSSSSSQVL LSPNGTGAVK SDERSVVATA VTTDTSGIEV DTVTEAELKE NGFRSTRRTK 

       130        140        150        160        170        180 
LICTIGPATC GFEQLEALAV GGMNVARLNM CHGTRDWHRG VIRSVRRLNE EKGFAVAIMM 

       190        200        210        220        230        240 
DTEGSEIHMG DLGGEASAKA EDGEVWTFTV RAFDSSRPER TISVSYDGFA EDVRVGDELL 

       250        260        270        280        290        300 
VDGGMVRFEV IEKIGPDVKC LCTDPGLLLP RANLTFWRDG SLVRERNAML PTISSKDWLD 

       310        320        330        340        350        360 
IDFGIAEGVD FIAVSFVKSA EVINHLKSYL AARSRGGEIG VIAKIESIDS LTNLEEIILA 

       370        380        390        400        410        420 
SDGAMVARGD LGAQIPLEQV PAAQQRIVQV CRALNKPVIV ASQLLESMIE YPTPTRAEVA 

       430        440        450        460        470        480 
DVSEAVRQRS DALMLSGESA MGQFPDKALT VLRTVSLRIE RWWREEKRHE SVPLQAIGSS 

       490        500        510        520        530        540 
FSDKISEEIC NSAAKMANNL GVDAVFVYTT SGHMASLVSR CRPDCPIFAF TTTTSVRRRL 

       550        560        570        580        590 
NLQWGLIPFR LSFSDDMESN LNKTFSLLKS RGMIKSGDLV IAVSDMLQSI QVMNVP 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions."
Kleffmann T., Russenberger D., von Zychlinski A., Christopher W., Sjoelander K., Gruissem W., Baginsky S.
Curr. Biol. 14:354-362(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"The oligomeric stromal proteome of Arabidopsis thaliana chloroplasts."
Peltier J.-B., Cai Y., Sun Q., Zabrouskov V., Giacomelli L., Rudella A., Ytterberg A.J., Rutschow H., van Wijk K.J.
Mol. Cell. Proteomics 5:114-133(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"The early responses of Arabidopsis thaliana cells to cadmium exposure explored by protein and metabolite profiling analyses."
Sarry J.-E., Kuhn L., Ducruix C., Lafaye A., Junot C., Hugouvieux V., Jourdain A., Bastien O., Fievet J.B., Vailhen D., Amekraz B., Moulin C., Ezan E., Garin J., Bourguignon J.
Proteomics 6:2180-2198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY CADMIUM [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.
[8]"A heteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis."
Andre C., Froehlich J.E., Moll M.R., Benning C.
Plant Cell 19:2006-2022(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, SUBUNIT, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY.
[9]"Function of plastidial pyruvate kinases in seeds of Arabidopsis thaliana."
Baud S., Wuilleme S., Dubreucq B., de Almeida A., Vuagnat C., Lepiniec L., Miquel M., Rochat C.
Plant J. 52:405-419(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY, NOMENCLATURE.
Strain: cv. Columbia and cv. Wassilewskija.
[10]"Arabidopsis seedlings deficient in a plastidic pyruvate kinase are unable to utilize seed storage compounds for germination and establishment."
Andre C., Benning C.
Plant Physiol. 145:1670-1680(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[11]"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[12]"AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[13]"Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
Olinares P.D., Ponnala L., van Wijk K.J.
Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP001300 Genomic DNA. Translation: BAB03043.1.
CP002686 Genomic DNA. Translation: AEE76697.1.
AY056196 mRNA. Translation: AAL07045.2.
AY056793 mRNA. Translation: AAL10484.1.
AY058084 mRNA. Translation: AAL24192.1.
BT002329 mRNA. Translation: AAN86162.1.
AY085149 mRNA. Translation: AAM61702.1.
RefSeqNP_566720.1. NM_113196.2.
UniGeneAt.5909.
At.74807.
At.75004.

3D structure databases

ProteinModelPortalQ9LIK0.
SMRQ9LIK0. Positions 117-581.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9LIK0. 30 interactions.
STRING3702.AT3G22960.1-P.

Proteomic databases

PRIDEQ9LIK0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G22960.1; AT3G22960.1; AT3G22960.
GeneID821870.
KEGGath:AT3G22960.

Organism-specific databases

TAIRAT3G22960.

Phylogenomic databases

eggNOGCOG0469.
HOGENOMHOG000021559.
InParanoidQ9LIK0.
KOK00873.
OMAYTSIRAS.
PhylomeDBQ9LIK0.

Enzyme and pathway databases

SABIO-RKQ9LIK0.
UniPathwayUPA00109; UER00188.

Gene expression databases

GenevestigatorQ9LIK0.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePKP1_ARATH
AccessionPrimary (citable) accession number: Q9LIK0
Secondary accession number(s): Q8LEY6, Q93ZY0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names