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Q9LIK0

- PKP1_ARATH

UniProt

Q9LIK0 - PKP1_ARATH

Protein

Plastidial pyruvate kinase 1, chloroplastic

Gene

PKP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Required for plastidial pyruvate kinase activity. Involved in seed oil accumulation, embryo development and seed storage compounds mobilization upon germination.2 Publications

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.1 Publication
    Potassium.1 Publication

    pH dependencei

    Optimum pH is 7.8-8.2.2 Publications

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei147 – 1471SubstrateBy similarity
    Metal bindingi149 – 1491PotassiumBy similarity
    Metal bindingi181 – 1811PotassiumBy similarity
    Metal bindingi182 – 1821Potassium; via carbonyl oxygenBy similarity
    Sitei344 – 3441Transition state stabilizerBy similarity
    Metal bindingi346 – 3461MagnesiumSequence Analysis
    Binding sitei369 – 3691Substrate; via amide nitrogenBy similarity
    Metal bindingi370 – 3701MagnesiumBy similarity
    Binding sitei370 – 3701Substrate; via amide nitrogenBy similarity
    Binding sitei402 – 4021SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: UniProtKB
    3. potassium ion binding Source: UniProtKB
    4. pyruvate kinase activity Source: TAIR

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway
    2. lipid metabolic process Source: TAIR
    3. response to cadmium ion Source: TAIR
    4. seed maturation Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    SABIO-RKQ9LIK0.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Plastidial pyruvate kinase 1, chloroplastic (EC:2.7.1.40)
    Short name:
    PK1
    Short name:
    PKp1
    Alternative name(s):
    Pyruvate kinase II
    Pyruvate kinase isozyme A
    Short name:
    PKP-ALPHA
    Gene namesi
    Name:PKP1
    Synonyms:PK1
    Ordered Locus Names:At3g22960
    ORF Names:F5N5.15
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G22960.

    Subcellular locationi

    Plastidchloroplast stroma 7 Publications

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Reduced plastidial pyruvate kinase activity and altered seed oil content leading to wrinkled seeds, retarded embryo elongation and reduced seed germination.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 8787ChloroplastBy similarityAdd
    BLAST
    Chaini88 – 596509Plastidial pyruvate kinase 1, chloroplasticPRO_0000416987Add
    BLAST

    Proteomic databases

    PRIDEiQ9LIK0.

    Expressioni

    Tissue specificityi

    Mostly expressed in seeds, and, to a lower extent, in roots, leaves (veins and trichomes), stems, inflorescences, siliques, pollen (grains and tubes) and flowers (sepals and petals).2 Publications

    Developmental stagei

    In seeds, accumulates in endosperm and embryo. In torpedo-shaped embryos, restricted to the hypocotyl and in the outer parts of the young cotyledons. In later embryo stages, present in all tissues except root tips.1 Publication

    Inductioni

    Induced by cadmium.1 Publication

    Gene expression databases

    GenevestigatoriQ9LIK0.

    Interactioni

    Subunit structurei

    Oligomer of alpha and beta subunits.1 Publication

    Protein-protein interaction databases

    IntActiQ9LIK0. 30 interactions.
    STRINGi3702.AT3G22960.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LIK0.
    SMRiQ9LIK0. Positions 117-581.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021559.
    InParanoidiQ9LIK0.
    KOiK00873.
    OMAiYTSIRAS.
    PhylomeDBiQ9LIK0.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9LIK0-1 [UniParc]FASTAAdd to Basket

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    MSQSIQFSTP SHTPHLLHLP HSQFNRPLSS ISFRRFPLTT IKYTSIRASS    50
    SSSPSPDLDS SSSSSSSQVL LSPNGTGAVK SDERSVVATA VTTDTSGIEV 100
    DTVTEAELKE NGFRSTRRTK LICTIGPATC GFEQLEALAV GGMNVARLNM 150
    CHGTRDWHRG VIRSVRRLNE EKGFAVAIMM DTEGSEIHMG DLGGEASAKA 200
    EDGEVWTFTV RAFDSSRPER TISVSYDGFA EDVRVGDELL VDGGMVRFEV 250
    IEKIGPDVKC LCTDPGLLLP RANLTFWRDG SLVRERNAML PTISSKDWLD 300
    IDFGIAEGVD FIAVSFVKSA EVINHLKSYL AARSRGGEIG VIAKIESIDS 350
    LTNLEEIILA SDGAMVARGD LGAQIPLEQV PAAQQRIVQV CRALNKPVIV 400
    ASQLLESMIE YPTPTRAEVA DVSEAVRQRS DALMLSGESA MGQFPDKALT 450
    VLRTVSLRIE RWWREEKRHE SVPLQAIGSS FSDKISEEIC NSAAKMANNL 500
    GVDAVFVYTT SGHMASLVSR CRPDCPIFAF TTTTSVRRRL NLQWGLIPFR 550
    LSFSDDMESN LNKTFSLLKS RGMIKSGDLV IAVSDMLQSI QVMNVP 596
    Length:596
    Mass (Da):65,131
    Last modified:October 1, 2000 - v1
    Checksum:i256CE329639A4BFD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti9 – 91T → S in AAM61702. 1 PublicationCurated
    Sequence conflicti52 – 521S → P in AAM61702. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP001300 Genomic DNA. Translation: BAB03043.1.
    CP002686 Genomic DNA. Translation: AEE76697.1.
    AY056196 mRNA. Translation: AAL07045.2.
    AY056793 mRNA. Translation: AAL10484.1.
    AY058084 mRNA. Translation: AAL24192.1.
    BT002329 mRNA. Translation: AAN86162.1.
    AY085149 mRNA. Translation: AAM61702.1.
    RefSeqiNP_566720.1. NM_113196.2.
    UniGeneiAt.5909.
    At.74807.
    At.75004.

    Genome annotation databases

    EnsemblPlantsiAT3G22960.1; AT3G22960.1; AT3G22960.
    GeneIDi821870.
    KEGGiath:AT3G22960.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP001300 Genomic DNA. Translation: BAB03043.1 .
    CP002686 Genomic DNA. Translation: AEE76697.1 .
    AY056196 mRNA. Translation: AAL07045.2 .
    AY056793 mRNA. Translation: AAL10484.1 .
    AY058084 mRNA. Translation: AAL24192.1 .
    BT002329 mRNA. Translation: AAN86162.1 .
    AY085149 mRNA. Translation: AAM61702.1 .
    RefSeqi NP_566720.1. NM_113196.2.
    UniGenei At.5909.
    At.74807.
    At.75004.

    3D structure databases

    ProteinModelPortali Q9LIK0.
    SMRi Q9LIK0. Positions 117-581.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9LIK0. 30 interactions.
    STRINGi 3702.AT3G22960.1-P.

    Proteomic databases

    PRIDEi Q9LIK0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G22960.1 ; AT3G22960.1 ; AT3G22960 .
    GeneIDi 821870.
    KEGGi ath:AT3G22960.

    Organism-specific databases

    TAIRi AT3G22960.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000021559.
    InParanoidi Q9LIK0.
    KOi K00873.
    OMAi YTSIRAS.
    PhylomeDBi Q9LIK0.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    SABIO-RK Q9LIK0.

    Gene expression databases

    Genevestigatori Q9LIK0.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
      DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions."
      Kleffmann T., Russenberger D., von Zychlinski A., Christopher W., Sjoelander K., Gruissem W., Baginsky S.
      Curr. Biol. 14:354-362(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. "The early responses of Arabidopsis thaliana cells to cadmium exposure explored by protein and metabolite profiling analyses."
      Sarry J.-E., Kuhn L., Ducruix C., Lafaye A., Junot C., Hugouvieux V., Jourdain A., Bastien O., Fievet J.B., Vailhen D., Amekraz B., Moulin C., Ezan E., Garin J., Bourguignon J.
      Proteomics 6:2180-2198(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY CADMIUM [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.
    8. "A heteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis."
      Andre C., Froehlich J.E., Moll M.R., Benning C.
      Plant Cell 19:2006-2022(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, SUBUNIT, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY.
    9. "Function of plastidial pyruvate kinases in seeds of Arabidopsis thaliana."
      Baud S., Wuilleme S., Dubreucq B., de Almeida A., Vuagnat C., Lepiniec L., Miquel M., Rochat C.
      Plant J. 52:405-419(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY, NOMENCLATURE.
      Strain: cv. Columbia and cv. Wassilewskija.
    10. "Arabidopsis seedlings deficient in a plastidic pyruvate kinase are unable to utilize seed storage compounds for germination and establishment."
      Andre C., Benning C.
      Plant Physiol. 145:1670-1680(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    11. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
      Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
      PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    12. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
      Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
      Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    13. "Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
      Olinares P.D., Ponnala L., van Wijk K.J.
      Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiPKP1_ARATH
    AccessioniPrimary (citable) accession number: Q9LIK0
    Secondary accession number(s): Q8LEY6, Q93ZY0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2012
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3