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Q9LIK0

- PKP1_ARATH

UniProt

Q9LIK0 - PKP1_ARATH

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Protein

Plastidial pyruvate kinase 1, chloroplastic

Gene
PKP1, PK1, At3g22960, F5N5.15
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for plastidial pyruvate kinase activity. Involved in seed oil accumulation, embryo development and seed storage compounds mobilization upon germination.2 Publications

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.1 Publication
Potassium.1 Publication

pH dependencei

Optimum pH is 7.8-8.2.2 Publications

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei147 – 1471Substrate By similarity
Metal bindingi149 – 1491Potassium By similarity
Metal bindingi181 – 1811Potassium By similarity
Metal bindingi182 – 1821Potassium; via carbonyl oxygen By similarity
Sitei344 – 3441Transition state stabilizer By similarity
Metal bindingi346 – 3461Magnesium Reviewed prediction
Binding sitei369 – 3691Substrate; via amide nitrogen By similarity
Metal bindingi370 – 3701Magnesium By similarity
Binding sitei370 – 3701Substrate; via amide nitrogen By similarity
Binding sitei402 – 4021Substrate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: UniProtKB
  3. potassium ion binding Source: UniProtKB
  4. pyruvate kinase activity Source: TAIR

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
  2. lipid metabolic process Source: TAIR
  3. response to cadmium ion Source: TAIR
  4. seed maturation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

SABIO-RKQ9LIK0.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Plastidial pyruvate kinase 1, chloroplastic (EC:2.7.1.40)
Short name:
PK1
Short name:
PKp1
Alternative name(s):
Pyruvate kinase II
Pyruvate kinase isozyme A
Short name:
PKP-ALPHA
Gene namesi
Name:PKP1
Synonyms:PK1
Ordered Locus Names:At3g22960
ORF Names:F5N5.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G22960.

Subcellular locationi

Plastidchloroplast stroma 7 Publications

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Reduced plastidial pyruvate kinase activity and altered seed oil content leading to wrinkled seeds, retarded embryo elongation and reduced seed germination.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 8787Chloroplast By similarityAdd
BLAST
Chaini88 – 596509Plastidial pyruvate kinase 1, chloroplasticPRO_0000416987Add
BLAST

Proteomic databases

PRIDEiQ9LIK0.

Expressioni

Tissue specificityi

Mostly expressed in seeds, and, to a lower extent, in roots, leaves (veins and trichomes), stems, inflorescences, siliques, pollen (grains and tubes) and flowers (sepals and petals).2 Publications

Developmental stagei

In seeds, accumulates in endosperm and embryo. In torpedo-shaped embryos, restricted to the hypocotyl and in the outer parts of the young cotyledons. In later embryo stages, present in all tissues except root tips.1 Publication

Inductioni

Induced by cadmium.

Gene expression databases

GenevestigatoriQ9LIK0.

Interactioni

Subunit structurei

Oligomer of alpha and beta subunits.1 Publication

Protein-protein interaction databases

IntActiQ9LIK0. 30 interactions.
STRINGi3702.AT3G22960.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9LIK0.
SMRiQ9LIK0. Positions 117-581.

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021559.
InParanoidiQ9LIK0.
KOiK00873.
OMAiYTSIRAS.
PhylomeDBiQ9LIK0.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LIK0-1 [UniParc]FASTAAdd to Basket

« Hide

MSQSIQFSTP SHTPHLLHLP HSQFNRPLSS ISFRRFPLTT IKYTSIRASS    50
SSSPSPDLDS SSSSSSSQVL LSPNGTGAVK SDERSVVATA VTTDTSGIEV 100
DTVTEAELKE NGFRSTRRTK LICTIGPATC GFEQLEALAV GGMNVARLNM 150
CHGTRDWHRG VIRSVRRLNE EKGFAVAIMM DTEGSEIHMG DLGGEASAKA 200
EDGEVWTFTV RAFDSSRPER TISVSYDGFA EDVRVGDELL VDGGMVRFEV 250
IEKIGPDVKC LCTDPGLLLP RANLTFWRDG SLVRERNAML PTISSKDWLD 300
IDFGIAEGVD FIAVSFVKSA EVINHLKSYL AARSRGGEIG VIAKIESIDS 350
LTNLEEIILA SDGAMVARGD LGAQIPLEQV PAAQQRIVQV CRALNKPVIV 400
ASQLLESMIE YPTPTRAEVA DVSEAVRQRS DALMLSGESA MGQFPDKALT 450
VLRTVSLRIE RWWREEKRHE SVPLQAIGSS FSDKISEEIC NSAAKMANNL 500
GVDAVFVYTT SGHMASLVSR CRPDCPIFAF TTTTSVRRRL NLQWGLIPFR 550
LSFSDDMESN LNKTFSLLKS RGMIKSGDLV IAVSDMLQSI QVMNVP 596
Length:596
Mass (Da):65,131
Last modified:October 1, 2000 - v1
Checksum:i256CE329639A4BFD
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91T → S in AAM61702. 1 Publication
Sequence conflicti52 – 521S → P in AAM61702. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP001300 Genomic DNA. Translation: BAB03043.1.
CP002686 Genomic DNA. Translation: AEE76697.1.
AY056196 mRNA. Translation: AAL07045.2.
AY056793 mRNA. Translation: AAL10484.1.
AY058084 mRNA. Translation: AAL24192.1.
BT002329 mRNA. Translation: AAN86162.1.
AY085149 mRNA. Translation: AAM61702.1.
RefSeqiNP_566720.1. NM_113196.2.
UniGeneiAt.5909.
At.74807.
At.75004.

Genome annotation databases

EnsemblPlantsiAT3G22960.1; AT3G22960.1; AT3G22960.
GeneIDi821870.
KEGGiath:AT3G22960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP001300 Genomic DNA. Translation: BAB03043.1 .
CP002686 Genomic DNA. Translation: AEE76697.1 .
AY056196 mRNA. Translation: AAL07045.2 .
AY056793 mRNA. Translation: AAL10484.1 .
AY058084 mRNA. Translation: AAL24192.1 .
BT002329 mRNA. Translation: AAN86162.1 .
AY085149 mRNA. Translation: AAM61702.1 .
RefSeqi NP_566720.1. NM_113196.2.
UniGenei At.5909.
At.74807.
At.75004.

3D structure databases

ProteinModelPortali Q9LIK0.
SMRi Q9LIK0. Positions 117-581.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9LIK0. 30 interactions.
STRINGi 3702.AT3G22960.1-P.

Proteomic databases

PRIDEi Q9LIK0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G22960.1 ; AT3G22960.1 ; AT3G22960 .
GeneIDi 821870.
KEGGi ath:AT3G22960.

Organism-specific databases

TAIRi AT3G22960.

Phylogenomic databases

eggNOGi COG0469.
HOGENOMi HOG000021559.
InParanoidi Q9LIK0.
KOi K00873.
OMAi YTSIRAS.
PhylomeDBi Q9LIK0.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
SABIO-RK Q9LIK0.

Gene expression databases

Genevestigatori Q9LIK0.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions."
    Kleffmann T., Russenberger D., von Zychlinski A., Christopher W., Sjoelander K., Gruissem W., Baginsky S.
    Curr. Biol. 14:354-362(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. "The early responses of Arabidopsis thaliana cells to cadmium exposure explored by protein and metabolite profiling analyses."
    Sarry J.-E., Kuhn L., Ducruix C., Lafaye A., Junot C., Hugouvieux V., Jourdain A., Bastien O., Fievet J.B., Vailhen D., Amekraz B., Moulin C., Ezan E., Garin J., Bourguignon J.
    Proteomics 6:2180-2198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY CADMIUM [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  8. "A heteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis."
    Andre C., Froehlich J.E., Moll M.R., Benning C.
    Plant Cell 19:2006-2022(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, SUBUNIT, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY.
  9. "Function of plastidial pyruvate kinases in seeds of Arabidopsis thaliana."
    Baud S., Wuilleme S., Dubreucq B., de Almeida A., Vuagnat C., Lepiniec L., Miquel M., Rochat C.
    Plant J. 52:405-419(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia and cv. Wassilewskija.
  10. "Arabidopsis seedlings deficient in a plastidic pyruvate kinase are unable to utilize seed storage compounds for germination and establishment."
    Andre C., Benning C.
    Plant Physiol. 145:1670-1680(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  11. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  12. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
    Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
    Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  13. "Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
    Olinares P.D., Ponnala L., van Wijk K.J.
    Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiPKP1_ARATH
AccessioniPrimary (citable) accession number: Q9LIK0
Secondary accession number(s): Q8LEY6, Q93ZY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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