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Q9LIK0

- PKP1_ARATH

UniProt

Q9LIK0 - PKP1_ARATH

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Protein

Plastidial pyruvate kinase 1, chloroplastic

Gene

PKP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for plastidial pyruvate kinase activity. Involved in seed oil accumulation, embryo development and seed storage compounds mobilization upon germination.2 Publications

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+1 Publication
  • K(+)1 Publication

pH dependencei

Optimum pH is 7.8-8.2.2 Publications

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei147 – 1471SubstrateBy similarity
Metal bindingi149 – 1491PotassiumBy similarity
Metal bindingi181 – 1811PotassiumBy similarity
Metal bindingi182 – 1821Potassium; via carbonyl oxygenBy similarity
Sitei344 – 3441Transition state stabilizerBy similarity
Metal bindingi346 – 3461MagnesiumSequence Analysis
Binding sitei369 – 3691Substrate; via amide nitrogenBy similarity
Metal bindingi370 – 3701MagnesiumBy similarity
Binding sitei370 – 3701Substrate; via amide nitrogenBy similarity
Binding sitei402 – 4021SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: UniProtKB
  3. potassium ion binding Source: UniProtKB
  4. pyruvate kinase activity Source: TAIR

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
  2. lipid metabolic process Source: TAIR
  3. response to cadmium ion Source: TAIR
  4. seed maturation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

ReactomeiREACT_239741. Regulation of gene expression in beta cells.
REACT_248260. Glycolysis.
REACT_260729. ChREBP activates metabolic gene expression.
SABIO-RKQ9LIK0.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Plastidial pyruvate kinase 1, chloroplastic (EC:2.7.1.40)
Short name:
PK1
Short name:
PKp1
Alternative name(s):
Pyruvate kinase II
Pyruvate kinase isozyme A
Short name:
PKP-ALPHA
Gene namesi
Name:PKP1
Synonyms:PK1
Ordered Locus Names:At3g22960
ORF Names:F5N5.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G22960.

Subcellular locationi

Plastidchloroplast stroma 7 Publications

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Reduced plastidial pyruvate kinase activity and altered seed oil content leading to wrinkled seeds, retarded embryo elongation and reduced seed germination.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 8787ChloroplastBy similarityAdd
BLAST
Chaini88 – 596509Plastidial pyruvate kinase 1, chloroplasticPRO_0000416987Add
BLAST

Proteomic databases

PRIDEiQ9LIK0.

Expressioni

Tissue specificityi

Mostly expressed in seeds, and, to a lower extent, in roots, leaves (veins and trichomes), stems, inflorescences, siliques, pollen (grains and tubes) and flowers (sepals and petals).2 Publications

Developmental stagei

In seeds, accumulates in endosperm and embryo. In torpedo-shaped embryos, restricted to the hypocotyl and in the outer parts of the young cotyledons. In later embryo stages, present in all tissues except root tips.1 Publication

Inductioni

Induced by cadmium.1 Publication

Gene expression databases

GenevestigatoriQ9LIK0.

Interactioni

Subunit structurei

Oligomer of alpha and beta subunits.1 Publication

Protein-protein interaction databases

IntActiQ9LIK0. 30 interactions.
STRINGi3702.AT3G22960.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9LIK0.
SMRiQ9LIK0. Positions 117-581.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021559.
InParanoidiQ9LIK0.
KOiK00873.
OMAiYTSIRAS.
PhylomeDBiQ9LIK0.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LIK0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQSIQFSTP SHTPHLLHLP HSQFNRPLSS ISFRRFPLTT IKYTSIRASS
60 70 80 90 100
SSSPSPDLDS SSSSSSSQVL LSPNGTGAVK SDERSVVATA VTTDTSGIEV
110 120 130 140 150
DTVTEAELKE NGFRSTRRTK LICTIGPATC GFEQLEALAV GGMNVARLNM
160 170 180 190 200
CHGTRDWHRG VIRSVRRLNE EKGFAVAIMM DTEGSEIHMG DLGGEASAKA
210 220 230 240 250
EDGEVWTFTV RAFDSSRPER TISVSYDGFA EDVRVGDELL VDGGMVRFEV
260 270 280 290 300
IEKIGPDVKC LCTDPGLLLP RANLTFWRDG SLVRERNAML PTISSKDWLD
310 320 330 340 350
IDFGIAEGVD FIAVSFVKSA EVINHLKSYL AARSRGGEIG VIAKIESIDS
360 370 380 390 400
LTNLEEIILA SDGAMVARGD LGAQIPLEQV PAAQQRIVQV CRALNKPVIV
410 420 430 440 450
ASQLLESMIE YPTPTRAEVA DVSEAVRQRS DALMLSGESA MGQFPDKALT
460 470 480 490 500
VLRTVSLRIE RWWREEKRHE SVPLQAIGSS FSDKISEEIC NSAAKMANNL
510 520 530 540 550
GVDAVFVYTT SGHMASLVSR CRPDCPIFAF TTTTSVRRRL NLQWGLIPFR
560 570 580 590
LSFSDDMESN LNKTFSLLKS RGMIKSGDLV IAVSDMLQSI QVMNVP
Length:596
Mass (Da):65,131
Last modified:October 1, 2000 - v1
Checksum:i256CE329639A4BFD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91T → S in AAM61702. 1 PublicationCurated
Sequence conflicti52 – 521S → P in AAM61702. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001300 Genomic DNA. Translation: BAB03043.1.
CP002686 Genomic DNA. Translation: AEE76697.1.
AY056196 mRNA. Translation: AAL07045.2.
AY056793 mRNA. Translation: AAL10484.1.
AY058084 mRNA. Translation: AAL24192.1.
BT002329 mRNA. Translation: AAN86162.1.
AY085149 mRNA. Translation: AAM61702.1.
RefSeqiNP_566720.1. NM_113196.2.
UniGeneiAt.5909.
At.74807.
At.75004.

Genome annotation databases

EnsemblPlantsiAT3G22960.1; AT3G22960.1; AT3G22960.
GeneIDi821870.
KEGGiath:AT3G22960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001300 Genomic DNA. Translation: BAB03043.1 .
CP002686 Genomic DNA. Translation: AEE76697.1 .
AY056196 mRNA. Translation: AAL07045.2 .
AY056793 mRNA. Translation: AAL10484.1 .
AY058084 mRNA. Translation: AAL24192.1 .
BT002329 mRNA. Translation: AAN86162.1 .
AY085149 mRNA. Translation: AAM61702.1 .
RefSeqi NP_566720.1. NM_113196.2.
UniGenei At.5909.
At.74807.
At.75004.

3D structure databases

ProteinModelPortali Q9LIK0.
SMRi Q9LIK0. Positions 117-581.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9LIK0. 30 interactions.
STRINGi 3702.AT3G22960.1-P.

Proteomic databases

PRIDEi Q9LIK0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G22960.1 ; AT3G22960.1 ; AT3G22960 .
GeneIDi 821870.
KEGGi ath:AT3G22960.

Organism-specific databases

TAIRi AT3G22960.

Phylogenomic databases

eggNOGi COG0469.
HOGENOMi HOG000021559.
InParanoidi Q9LIK0.
KOi K00873.
OMAi YTSIRAS.
PhylomeDBi Q9LIK0.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
Reactomei REACT_239741. Regulation of gene expression in beta cells.
REACT_248260. Glycolysis.
REACT_260729. ChREBP activates metabolic gene expression.
SABIO-RK Q9LIK0.

Gene expression databases

Genevestigatori Q9LIK0.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions."
    Kleffmann T., Russenberger D., von Zychlinski A., Christopher W., Sjoelander K., Gruissem W., Baginsky S.
    Curr. Biol. 14:354-362(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. "The early responses of Arabidopsis thaliana cells to cadmium exposure explored by protein and metabolite profiling analyses."
    Sarry J.-E., Kuhn L., Ducruix C., Lafaye A., Junot C., Hugouvieux V., Jourdain A., Bastien O., Fievet J.B., Vailhen D., Amekraz B., Moulin C., Ezan E., Garin J., Bourguignon J.
    Proteomics 6:2180-2198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY CADMIUM [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  8. "A heteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis."
    Andre C., Froehlich J.E., Moll M.R., Benning C.
    Plant Cell 19:2006-2022(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, SUBUNIT, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY.
  9. "Function of plastidial pyruvate kinases in seeds of Arabidopsis thaliana."
    Baud S., Wuilleme S., Dubreucq B., de Almeida A., Vuagnat C., Lepiniec L., Miquel M., Rochat C.
    Plant J. 52:405-419(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia and cv. Wassilewskija.
  10. "Arabidopsis seedlings deficient in a plastidic pyruvate kinase are unable to utilize seed storage compounds for germination and establishment."
    Andre C., Benning C.
    Plant Physiol. 145:1670-1680(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  11. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  12. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
    Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
    Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  13. "Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
    Olinares P.D., Ponnala L., van Wijk K.J.
    Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiPKP1_ARATH
AccessioniPrimary (citable) accession number: Q9LIK0
Secondary accession number(s): Q8LEY6, Q93ZY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: October 1, 2000
Last modified: November 26, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3