Plastidial pyruvate kinase 1, chloroplastic precursor - Arabidopsis thaliana (Mouse-ear cress)

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Q9LIK0 (PKP1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 97. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Plastidial pyruvate kinase 1, chloroplastic
Short name=PK1
Short name=PKp1
EC=2.7.1.40

Alternative name(s):
Pyruvate kinase II
Pyruvate kinase isozyme A
Short name=PKP-ALPHA

Gene names

Name:	PKP1
Synonyms:	PK1
Ordered Locus Names:	At3g22960
ORF Names:	F5N5.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for plastidial pyruvate kinase activity. Involved in seed oil accumulation, embryo development and seed storage compounds mobilization upon germination. Ref.9 Ref.10
Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium. Ref.8 Potassium. Ref.8
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Oligomer of alpha and beta subunits. Ref.8
Subcellular location	Plastid › chloroplast stroma Ref.5 Ref.6 Ref.8 Ref.9 Ref.11 Ref.12 Ref.13.
Tissue specificity	Mostly expressed in seeds, and, to a lower extent, in roots, leaves (veins and trichomes), stems, inflorescences, siliques, pollen (grains and tubes) and flowers (sepals and petals). Ref.8 Ref.9
Developmental stage	In seeds, accumulates in endosperm and embryo. In torpedo-shaped embryos, restricted to the hypocotyl and in the outer parts of the young cotyledons. In later embryo stages, present in all tissues except root tips. Ref.9
Induction	Induced by cadmium.
Disruption phenotype	Reduced plastidial pyruvate kinase activity and altered seed oil content leading to wrinkled seeds, retarded embryo elongation and reduced seed germination. Ref.9 Ref.10
Sequence similarities	Belongs to the pyruvate kinase family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.8-8.2. Ref.8 Ref.9

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Pyruvate
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway lipid metabolic process Inferred from genetic interaction PubMed 19755540. Source: TAIR response to cadmium ion Inferred from expression pattern Ref.7. Source: TAIR seed maturation Inferred from mutant phenotype Ref.9. Source: UniProtKB
Cellular_component	chloroplast stroma Inferred from direct assay Ref.6 Ref.8 Ref.12. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from direct assay Ref.8. Source: UniProtKB potassium ion binding Inferred from direct assay Ref.8. Source: UniProtKB pyruvate kinase activity Inferred from direct assay Ref.8. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 87

Chloroplast By similarity

Chain

88 – 596

509

Plastidial pyruvate kinase 1, chloroplastic

PRO_0000416987

Sites

Metal binding

149

Potassium By similarity

Metal binding

181

Potassium By similarity

Metal binding

182

Potassium; via carbonyl oxygen By similarity

Metal binding

346

Magnesium Potential

Metal binding

370

Magnesium By similarity

Binding site

147

Substrate By similarity

Binding site

369

Substrate; via amide nitrogen By similarity

Binding site

370

Substrate; via amide nitrogen By similarity

Binding site

402

Substrate By similarity

Site

344

Transition state stabilizer By similarity

Experimental info

Sequence conflict

T → S in AAM61702. Ref.4

Sequence conflict

S → P in AAM61702. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

Q9LIK0 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 256CE329639A4BFD
Show »

FASTA

596

65,131

        10         20         30         40         50         60 
MSQSIQFSTP SHTPHLLHLP HSQFNRPLSS ISFRRFPLTT IKYTSIRASS SSSPSPDLDS 

        70         80         90        100        110        120 
SSSSSSSQVL LSPNGTGAVK SDERSVVATA VTTDTSGIEV DTVTEAELKE NGFRSTRRTK 

       130        140        150        160        170        180 
LICTIGPATC GFEQLEALAV GGMNVARLNM CHGTRDWHRG VIRSVRRLNE EKGFAVAIMM 

       190        200        210        220        230        240 
DTEGSEIHMG DLGGEASAKA EDGEVWTFTV RAFDSSRPER TISVSYDGFA EDVRVGDELL 

       250        260        270        280        290        300 
VDGGMVRFEV IEKIGPDVKC LCTDPGLLLP RANLTFWRDG SLVRERNAML PTISSKDWLD 

       310        320        330        340        350        360 
IDFGIAEGVD FIAVSFVKSA EVINHLKSYL AARSRGGEIG VIAKIESIDS LTNLEEIILA 

       370        380        390        400        410        420 
SDGAMVARGD LGAQIPLEQV PAAQQRIVQV CRALNKPVIV ASQLLESMIE YPTPTRAEVA 

       430        440        450        460        470        480 
DVSEAVRQRS DALMLSGESA MGQFPDKALT VLRTVSLRIE RWWREEKRHE SVPLQAIGSS 

       490        500        510        520        530        540 
FSDKISEEIC NSAAKMANNL GVDAVFVYTT SGHMASLVSR CRPDCPIFAF TTTTSVRRRL 

       550        560        570        580        590 
NLQWGLIPFR LSFSDDMESN LNKTFSLLKS RGMIKSGDLV IAVSDMLQSI QVMNVP

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The Arabidopsis thaliana chloroplast proteome reveals pathway abundance and novel protein functions." Kleffmann T., Russenberger D., von Zychlinski A., Christopher W., Sjoelander K., Gruissem W., Baginsky S. Curr. Biol. 14:354-362(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]	"The oligomeric stromal proteome of Arabidopsis thaliana chloroplasts." Peltier J.-B., Cai Y., Sun Q., Zabrouskov V., Giacomelli L., Rudella A., Ytterberg A.J., Rutschow H., van Wijk K.J. Mol. Cell. Proteomics 5:114-133(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"The early responses of Arabidopsis thaliana cells to cadmium exposure explored by protein and metabolite profiling analyses." Sarry J.-E., Kuhn L., Ducruix C., Lafaye A., Junot C., Hugouvieux V., Jourdain A., Bastien O., Fievet J.B., Vailhen D., Amekraz B., Moulin C., Ezan E., Garin J., Bourguignon J. Proteomics 6:2180-2198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY CADMIUM [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: cv. Columbia.
[8]	"A heteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis." Andre C., Froehlich J.E., Moll M.R., Benning C. Plant Cell 19:2006-2022(2007) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, SUBUNIT, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY.
[9]	"Function of plastidial pyruvate kinases in seeds of Arabidopsis thaliana." Baud S., Wuilleme S., Dubreucq B., de Almeida A., Vuagnat C., Lepiniec L., Miquel M., Rochat C. Plant J. 52:405-419(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES, GENE FAMILY, NOMENCLATURE. Strain: cv. Columbia and cv. Wassilewskija.
[10]	"Arabidopsis seedlings deficient in a plastidic pyruvate kinase are unable to utilize seed storage compounds for germination and establishment." Andre C., Benning C. Plant Physiol. 145:1670-1680(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[11]	"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome." Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J. PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins." Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N. Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]	"Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering." Olinares P.D., Ponnala L., van Wijk K.J. Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP001300 Genomic DNA. Translation: BAB03043.1. CP002686 Genomic DNA. Translation: AEE76697.1. AY056196 mRNA. Translation: AAL07045.2. AY056793 mRNA. Translation: AAL10484.1. AY058084 mRNA. Translation: AAL24192.1. BT002329 mRNA. Translation: AAN86162.1. AY085149 mRNA. Translation: AAM61702.1.
IPI	IPI00534572.
RefSeq	NP_566720.1. NM_113196.2.
UniGene	At.5909. At.74807. At.75004.
3D structure databases
HSSP	HSSP built from PDB template 1E0T based on UniProtKB P14178.
ProteinModelPortal	Q9LIK0.
SMR	Q9LIK0. Positions 117-581.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LIK0. 30 interactions.
STRING	3702.AT3G22960.1-P.
Proteomic databases
PRIDE	Q9LIK0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G22960.1; AT3G22960.1; AT3G22960.
GeneID	821870.
KEGG	ath:AT3G22960.
Organism-specific databases
TAIR	At3g22960.
Phylogenomic databases
eggNOG	COG0469.
HOGENOM	HOG000021559.
InParanoid	Q9LIK0.
KO	K00873.
OMA	SSISFRR.
PhylomeDB	Q9LIK0.
ProtClustDB	PLN02762.
Enzyme and pathway databases
SABIO-RK	Q9LIK0.
UniPathway	UPA00109; UER00188.
Gene expression databases
ArrayExpress	Q9LIK0.
Genevestigator	Q9LIK0.
Family and domain databases
Gene3D	2.40.33.10. 1 hit. 3.20.20.60. 2 hits. 3.40.1380.20. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase. IPR011037. Pyrv_Knase-like_insert_dom. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR015806. Pyrv_Knase_insert_dom. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
SUPFAM	SSF50800. PK_B_barrel_like. 1 hit. SSF52935. Pyruvate_kinase. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PKP1_ARATH

Accession

Primary (citable) accession number: Q9LIK0
Secondary accession number(s): Q8LEY6, Q93ZY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2012
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents