Q9LIF9 (BGL19_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-glucosidase 19
Short name=AtBGLU19
EC=3.2.1.21

Gene names

Name:	BGLU19
Ordered Locus Names:	At3g21370
ORF Names:	MHC9.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
Subcellular location	Endoplasmic reticulum lumen Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 527	506	Beta-glucosidase 19		PRO_0000389581
Regions
	Region	476 – 477	2	Substrate binding By similarity
	Motif	524 – 527	4	Prevents secretion from ER Potential
Sites
	Active site	201	1	Proton donor By similarity
	Active site	418	1	Nucleophile By similarity
	Binding site	52	1	Substrate By similarity
	Binding site	155	1	Substrate By similarity
	Binding site	200	1	Substrate By similarity
	Binding site	345	1	Substrate By similarity
	Binding site	469	1	Substrate By similarity
Amino acid modifications
	Glycosylation	183	1	N-linked (GlcNAc...) Potential
	Glycosylation	462	1	N-linked (GlcNAc...) Potential
	Glycosylation	495	1	N-linked (GlcNAc...) Potential
	Disulfide bond	220 ↔ 231		By similarity
Experimental info
	Sequence conflict	349	1	F → L in AAL24252. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9LIF9 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A9EB34BC818BF88A	FASTA	527	60,015
	10 20 30 40 50 60 MKIPLLGLLL LISLVGSPTR AEEGPVCPKT ETLSRASFPE GFMFGTATAA FQVEGAVNEG 70 80 90 100 110 120 CRGPSLWDIY TKKFPHRVKN HNADEAVDFY HRYKEDIQLM KKLNTDGFRL SISWPRIFPH 130 140 150 160 170 180 GRMEKGISKE GVQFYHDLID ELLKNDITPL VTVFHWDTPA DLEDEYGGFL SERIVPDFVE 190 200 210 220 230 240 YANFTFHEYG DKVKNWITFN EPWVFSRSGY DVGKKAPGRC SPYVKEFGKL CQDGRSGFEP 250 260 270 280 290 300 YVVSHNLLVG HAEAVDAFRK CEKCKGGKIG IAHSPAWFEP EDVEGGQATV NRVLDFVIGW 310 320 330 340 350 360 HLDPTTFGDY PQSMKDAVGS RLPRFTKAQK AKLKDSTDFV GINYYTSFFA KADQKVDSRN 370 380 390 400 410 420 PTWATDALVE FEPKTVDGSI KIGSQPNTAK MAVYAKGLRK LMKYIKDRYN SPEIIITENG 430 440 450 460 470 480 YGEDLGDKDT DLSVALNDHN RKYYLQRHLL ALNEAICEDK VNVTSYFLWS LMDNFEWQDG 490 500 510 520 YTARFGVYYI DFKNNLTRME KESAKWLSEF LKPGLKPSKS SKLHEEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1." Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R., Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A. Plant Mol. Biol. 55:343-367(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP001305 Genomic DNA. Translation: BAB03050.1. CP002686 Genomic DNA. Translation: AEE76501.1. AY058865 mRNA. Translation: AAL24252.1. AY064046 mRNA. Translation: AAL36402.1. AY096383 mRNA. Translation: AAM20024.1.
IPI	IPI00524504.
RefSeq	NP_188774.2. NM_113032.3.
UniGene	At.47366. At.63567.
3D structure databases
HSSP	HSSP built from PDB template 1CBG based on UniProtKB P26205.
ProteinModelPortal	Q9LIF9.
SMR	Q9LIF9. Positions 33-512.
ModBase	Search...
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Proteomic databases
PaxDb	Q9LIF9.
PRIDE	Q9LIF9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G21370.1; AT3G21370.1; AT3G21370.
GeneID	821691.
KEGG	ath:AT3G21370.
Organism-specific databases
TAIR	At3g21370.
Phylogenomic databases
eggNOG	COG2723.
HOGENOM	HOG000088630.
InParanoid	Q9LIF9.
KO	K01188.
OMA	MIACHER.
PhylomeDB	Q9LIF9.
ProtClustDB	CLSN2917948.
Gene expression databases
ArrayExpress	Q9LIF9.
Genevestigator	Q9LIF9.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10353. PTHR10353. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00572. GLYCOSYL_HYDROL_F1_1. False negative. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGL19_ARATH

Accession

Primary (citable) accession number: Q9LIF9
Secondary accession number(s): Q93Z07

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 24, 2009
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program