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Protein

Probable protein S-acyltransferase 20

Gene

PAT20

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Palmitoyl acyltransferase.By similarity1 Publication

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei202S-palmitoyl cysteine intermediateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.225. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein S-acyltransferase 20 (EC:2.3.1.225)
Alternative name(s):
Probable palmitoyltransferase At3g22180
Zinc finger DHHC domain-containing protein At3g22180
Gene namesi
Name:PAT20
Ordered Locus Names:At3g22180
ORF Names:MKA23.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRilocus:2090404. AT3G22180.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei16 – 36HelicalSequence analysisAdd BLAST21
Transmembranei41 – 61HelicalSequence analysisAdd BLAST21
Transmembranei220 – 240HelicalSequence analysisAdd BLAST21
Transmembranei275 – 295HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003154051 – 706Probable protein S-acyltransferase 20Add BLAST706

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ9LIE4.

PTM databases

iPTMnetiQ9LIE4.

Expressioni

Gene expression databases

GenevisibleiQ9LIE4. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G22180.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LIE4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini172 – 222DHHCPROSITE-ProRule annotationAdd BLAST51

Domaini

The DHHC domain is required for palmitoyltransferase activity.By similarity

Sequence similaritiesi

Belongs to the DHHC palmitoyltransferase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1311. Eukaryota.
COG5273. LUCA.
HOGENOMiHOG000242421.
InParanoidiQ9LIE4.
KOiK20027.
OMAiPRIMSIF.
OrthoDBiEOG0936048N.
PhylomeDBiQ9LIE4.

Family and domain databases

InterProiView protein in InterPro
IPR001594. Palmitoyltrfase_DHHC.
PfamiView protein in Pfam
PF01529. zf-DHHC. 1 hit.
PROSITEiView protein in PROSITE
PS50216. DHHC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9LIE4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRKHGWQLP AHTLQVIAIT VFCLLVVAFY AFFAPFVGGR IWEYVLIGVY
60 70 80 90 100
SPVAILVFVL YVRCTAINPA DPRIMSIFDT GVNGDGMVRG LSRNYDETGS
110 120 130 140 150
QLQASPSVVS RSSTVAGNSS VKGSVEDAQR VESVSRRSCY NPLAVFCYVF
160 170 180 190 200
VVEDCRKKEG PAEEQGNSEE ALFCTLCNCE VRKFSKHCRS CDKCVDCFDH
210 220 230 240 250
HCKWLNNCVG RKNYVTFVSL MSASLLWLII EAAVGIAVIV RVFVNKQTME
260 270 280 290 300
TEIVNRLGNS FSRAPLAAVV GLCTAVAIFA CFPLGELLFF HMLLIKKGIT
310 320 330 340 350
TYEYVVAMRA MSEAPDGASV DEEIQNVLYS PTGSATTGFS GGSSLGLPYR
360 370 380 390 400
GVWCTPPRVF DNQDEVIPHL DPCMVPSTVD PDAPGSEKGT KALKRPVKRN
410 420 430 440 450
AWKLAKLDPN EAARAAARAR ASSSVLRPID NRHLPDNDLS SIGTVSIISS
460 470 480 490 500
VSTDANVAAS KEIRNNDLRS SLSRNSFAPS QGSRDEYDTG SHGMSNLSSP
510 520 530 540 550
SHVHESVTLA PLPQNPTIVG NRFTATSHHM HSTFDDKVLH RGNDADPLFL
560 570 580 590 600
FAPATSHLRD VRKTSVVWDP EAGRYVSAPV TTTSEVRNRL LNPSSQTAST
610 620 630 640 650
QNPRPILPAH DSSSGSSALR DPLPLHQAER RLTYTGDSIF YGGPLINIPT
660 670 680 690 700
RDTPRSGRGL VRDVQDRLAS TVHRDARIRR DSTSNQLPVF APGGLGANSQ

TGSNIK
Length:706
Mass (Da):76,874
Last modified:January 15, 2008 - v2
Checksum:iC4BBD759A9626B67
GO

Sequence cautioni

The sequence BAB03066 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001306 Genomic DNA. Translation: BAB03066.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76598.1.
RefSeqiNP_188857.1. NM_113115.4.
UniGeneiAt.37920.

Genome annotation databases

EnsemblPlantsiAT3G22180.1; AT3G22180.1; AT3G22180.
GeneIDi821782.
GrameneiAT3G22180.1; AT3G22180.1; AT3G22180.
KEGGiath:AT3G22180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001306 Genomic DNA. Translation: BAB03066.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76598.1.
RefSeqiNP_188857.1. NM_113115.4.
UniGeneiAt.37920.

3D structure databases

ProteinModelPortaliQ9LIE4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G22180.1.

PTM databases

iPTMnetiQ9LIE4.

Proteomic databases

PaxDbiQ9LIE4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G22180.1; AT3G22180.1; AT3G22180.
GeneIDi821782.
GrameneiAT3G22180.1; AT3G22180.1; AT3G22180.
KEGGiath:AT3G22180.

Organism-specific databases

AraportiAT3G22180.
TAIRilocus:2090404. AT3G22180.

Phylogenomic databases

eggNOGiKOG1311. Eukaryota.
COG5273. LUCA.
HOGENOMiHOG000242421.
InParanoidiQ9LIE4.
KOiK20027.
OMAiPRIMSIF.
OrthoDBiEOG0936048N.
PhylomeDBiQ9LIE4.

Enzyme and pathway databases

BRENDAi2.3.1.225. 399.

Miscellaneous databases

PROiPR:Q9LIE4.

Gene expression databases

GenevisibleiQ9LIE4. AT.

Family and domain databases

InterProiView protein in InterPro
IPR001594. Palmitoyltrfase_DHHC.
PfamiView protein in Pfam
PF01529. zf-DHHC. 1 hit.
PROSITEiView protein in PROSITE
PS50216. DHHC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiZDHC8_ARATH
AccessioniPrimary (citable) accession number: Q9LIE4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: April 12, 2017
This is version 99 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.