Q9LIB9 (INV5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-fructofuranosidase, insoluble isoenzyme CWINV5
EC=3.2.1.26

Alternative name(s):
Cell wall beta-fructosidase 5
Cell wall invertase 5
Short name=AtcwINV5
Sucrose hydrolase 5

Gene names

Name:	CWINV5
Ordered Locus Names:	At3g13784
ORF Names:	MMM17.25

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Subcellular location	Secreted › extracellular space › apoplast Probable. Secreted › cell wall Probable. Note: Associated to the cell wall Probable.
Tissue specificity	Expressed in flowers, and, to a lower extent, in leaves. Ref.3 Ref.4
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.
Sequence caution	The sequence AEE75413.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	sucrose biosynthetic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 572	549	Beta-fructofuranosidase, insoluble isoenzyme CWINV5		PRO_0000348351
Regions
	Region	54 – 57	4	Substrate binding By similarity
	Region	118 – 119	2	Substrate binding By similarity
	Region	184 – 185	2	Substrate binding By similarity
Sites
	Active site	57	1	By similarity
	Binding site	73	1	Substrate By similarity
	Binding site	239	1	Substrate By similarity
Amino acid modifications
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	152	1	N-linked (GlcNAc...) Probable
	Glycosylation	179	1	N-linked (GlcNAc...) Probable
	Glycosylation	333	1	N-linked (GlcNAc...) Probable
	Glycosylation	438	1	N-linked (GlcNAc...) Probable
	Disulfide bond	434 ↔ 481		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LIB9 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: 9538338DBD0DAC70	FASTA	572	64,603
	10 20 30 40 50 60 MANIVWCNIA MFLLVSLFLT DDAVVVLDAL DNVPNNIKNQ PYRTGYHFQP PKNWMNDPNG 70 80 90 100 110 120 PMIYKGIYHL FYQWNQNGAV MDVNKTVWGH ATSTDLINWI TLSPAIRPSR PSDINGCWSG 130 140 150 160 170 180 SVTILPNGKP VILYTGNDRY NRQVQNLVKP KNLTDPYLRH WTKSPENPLV TPSPVNHINS 190 200 210 220 230 240 SAFRDPTTAW FGRDGRWRIT TGSQEGRRGL AILHTSKDFV IWKQSPKPLH YHDGTGMWEC 250 260 270 280 290 300 PDFFPVARTD SRGLDTSFSS GPMVKHVLKV SLTDTFNDYY TIGTYDEVRD VYVPDKGFVQ 310 320 330 340 350 360 DETAPRYDYG KFYASKTFYD SVNQRRILWG WVNESSPEKD NIKKGWAGLQ AIPRKVWLDE 370 380 390 400 410 420 SGKRLVQWPV KEIERLRTTQ VKWGNKLLKG GSVMEVHGVT APQADVEVFF KVSGFDLEKA 430 440 450 460 470 480 DVIEPGWTDP QLICSQRNAS SGLGPFGLMV LASKNMEEYT SVNIRIFRAG ENSKEHVVVM 490 500 510 520 530 540 CSDQSTSSLE KGNDKTTYGA FLDISPYQPI SLRTLIDKSI VESFGGKGKT CITSRVYPKL 550 560 570 AIGERTHLFA FNKGSQNVNV LSLSAWSMKS SL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Roles of cell-wall invertases and monosaccharide transporters in the growth and development of Arabidopsis." Sherson S.M., Alford H.L., Forbes S.M., Wallace G., Smith S.M. J. Exp. Bot. 54:525-531(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
[4]	"Imaging photosynthesis in wounded leaves of Arabidopsis thaliana." Quilliam R.S., Swarbrick P.J., Scholes J.D., Rolfe S.A. J. Exp. Bot. 57:55-69(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP001307 Genomic DNA. Translation: BAB01929.1. CP002686 Genomic DNA. Translation: AEE75413.1. Sequence problems.
IPI	IPI00546671. IPI01019614.
RefSeq	NP_187994.1. NM_112231.1.
UniGene	At.53300.
3D structure databases
ProteinModelPortal	Q9LIB9.
SMR	Q9LIB9. Positions 39-571.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT3G13784.1-P.
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Proteomic databases
PaxDb	Q9LIB9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	820590.
KEGG	ath:AT3G13784.
Organism-specific databases
TAIR	At3g13784.
Phylogenomic databases
eggNOG	COG1621.
HOGENOM	HOG000181427.
InParanoid	Q9LIB9.
PhylomeDB	Q9LIB9.
ProtClustDB	CLSN2915521.
Gene expression databases
ArrayExpress	Q9LIB9.
Genevestigator	Q9LIB9.
Family and domain databases
Gene3D	2.115.10.20. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_beta-prop. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INV5_ARATH

Accession

Primary (citable) accession number: Q9LIB9
Secondary accession number(s): F4JEI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	May 1, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program