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Protein

Alpha-glucan phosphorylase 1

Gene

PHS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties (By similarity). May be not required for the degradation of starch, but the phosphorolysis of starch may play an important role in water stress tolerance.By similarity1 Publication

Catalytic activityi

((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.

Cofactori

GO - Molecular functioni

  • glycogen phosphorylase activity Source: GO_Central
  • phosphorylase activity Source: TAIR
  • pyridoxal phosphate binding Source: GO_Central

GO - Biological processi

  • glycogen catabolic process Source: GO_Central
  • response to temperature stimulus Source: TAIR
  • response to water deprivation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT3G29320-MONOMER.
MetaCyc:AT3G29320-MONOMER.
ReactomeiR-ATH-70221. Glycogen breakdown (glycogenolysis).

Protein family/group databases

CAZyiGT35. Glycosyltransferase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-glucan phosphorylase 1 (EC:2.4.1.1)
Short name:
AtPHS1
Alternative name(s):
Alpha-glucan phosphorylase, L isozyme
Starch phosphorylase L
Gene namesi
Name:PHS1
Ordered Locus Names:At3g29320
ORF Names:MUO10.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G29320.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Small white lesions on the tips or margins of fully expanded leaves.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6363ChloroplastSequence analysisAdd
BLAST
Chaini64 – 962899Alpha-glucan phosphorylase 1PRO_0000420843Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei808 – 8081N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PaxDbiQ9LIB2.
PRIDEiQ9LIB2.
ProMEXiQ9LIB2.

PTM databases

iPTMnetiQ9LIB2.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LIB2. baseline and differential.
GenevisibleiQ9LIB2. AT.

Interactioni

Protein-protein interaction databases

BioGridi7919. 2 interactions.
IntActiQ9LIB2. 1 interaction.
STRINGi3702.AT3G29320.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LIB2.
SMRiQ9LIB2. Positions 87-962.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi544 – 5496Poly-Glu

Sequence similaritiesi

Belongs to the glycogen phosphorylase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2099. Eukaryota.
COG0058. LUCA.
HOGENOMiHOG000278444.
InParanoidiQ9LIB2.
KOiK00688.
OMAiATYEYYN.
OrthoDBiEOG093601NB.
PhylomeDBiQ9LIB2.

Family and domain databases

InterProiIPR011833. Glycg_phsphrylas.
IPR000811. Glyco_trans_35.
[Graphical view]
PANTHERiPTHR11468. PTHR11468. 2 hits.
PfamiPF00343. Phosphorylase. 1 hit.
[Graphical view]
PIRSFiPIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMsiTIGR02093. P_ylase. 1 hit.
PROSITEiPS00102. PHOSPHORYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LIB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTMRISGVS TGAEVLIQCN SLSSLVSRRC DDGKWRTRMF PARNRDLRPS
60 70 80 90 100
PTRRSFLSVK SISSEPKAKV TDAVLDSEQE VFISSMNPFA PDAASVASSI
110 120 130 140 150
KYHAEFTPLF SPEKFELPKA FFATAQSVRD ALIMNWNATY EYYNRVNVKQ
160 170 180 190 200
AYYLSMEFLQ GRALSNAVGN LGLNSAYGDA LKRLGFDLES VASQEPDPAL
210 220 230 240 250
GNGGLGRLAS CFLDSMATLN YPAWGYGLRY KYGLFKQRIT KDGQEEAAED
260 270 280 290 300
WLELSNPWEI VRNDVSYPIK FYGKVVFGSD GKKRWIGGED IVAVAYDVPI
310 320 330 340 350
PGYKTKTTIN LRLWSTKAPS EDFDLSSYNS GKHTEAAEAL FNAEKICFVL
360 370 380 390 400
YPGDESTEGK ALRLKQQYTL CSASLQDIVA RFETRSGGNV NWEEFPEKVA
410 420 430 440 450
VQMNDTHPTL CIPELMRILM DLKGLSWEDA WKITQRTVAY TNHTVLPEAL
460 470 480 490 500
EKWSLELMEK LLPRHVEIIE KIDEELVRTI VSEYGTADPD LLEEKLKAMR
510 520 530 540 550
ILENVELPSA FADVIVKPVN KPVTAKDAQN GVKTEQEEEK TAGEEEEDEV
560 570 580 590 600
IPEPTVEPPK MVRMANLAVV GGHAVNGVAE IHSEIVKQDV FNDFVQLWPE
610 620 630 640 650
KFQNKTNGVT PRRWIRFCNP YLSDIITNWI GTEDWVLNTE KVAELRKFAD
660 670 680 690 700
NEDLQSEWRA AKKKNKLKVV SLIKERTGYT VSPDAMFDIQ IKRIHEYKRQ
710 720 730 740 750
LLNILGIVYR YKKMKEMSAS EREKAFVPRV CIFGGKAFAT YVQAKRIVKF
760 770 780 790 800
ITDVASTINH DPEIGDLLKV IFVPDYNVSV AELLIPASEL SQHISTAGME
810 820 830 840 850
ASGTSNMKFS MNGCVLIGTL DGANVEIREE VGEENFFLFG AKADQIVNLR
860 870 880 890 900
KERAEGKFVP DPTFEEVKKF VGSGVFGSNS YDELIGSLEG NEGFGRADYF
910 920 930 940 950
LVGKDFPSYI ECQEKVDEAY RDQKRWTRMS IMNTAGSFKF SSDRTIHEYA
960
KDIWNIKQVE LP
Length:962
Mass (Da):108,585
Last modified:October 1, 2000 - v1
Checksum:i54B3C4BBB076CFA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001309 Genomic DNA. Translation: BAB02576.1.
CP002686 Genomic DNA. Translation: AEE77567.1.
AY049235 mRNA. Translation: AAK83578.1.
BT003015 mRNA. Translation: AAO23580.1.
RefSeqiNP_189578.1. NM_113857.3.
UniGeneiAt.50847.
At.5745.

Genome annotation databases

EnsemblPlantsiAT3G29320.1; AT3G29320.1; AT3G29320.
GeneIDi822590.
GrameneiAT3G29320.1; AT3G29320.1; AT3G29320.
KEGGiath:AT3G29320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001309 Genomic DNA. Translation: BAB02576.1.
CP002686 Genomic DNA. Translation: AEE77567.1.
AY049235 mRNA. Translation: AAK83578.1.
BT003015 mRNA. Translation: AAO23580.1.
RefSeqiNP_189578.1. NM_113857.3.
UniGeneiAt.50847.
At.5745.

3D structure databases

ProteinModelPortaliQ9LIB2.
SMRiQ9LIB2. Positions 87-962.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7919. 2 interactions.
IntActiQ9LIB2. 1 interaction.
STRINGi3702.AT3G29320.1.

Protein family/group databases

CAZyiGT35. Glycosyltransferase Family 35.

PTM databases

iPTMnetiQ9LIB2.

Proteomic databases

PaxDbiQ9LIB2.
PRIDEiQ9LIB2.
ProMEXiQ9LIB2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G29320.1; AT3G29320.1; AT3G29320.
GeneIDi822590.
GrameneiAT3G29320.1; AT3G29320.1; AT3G29320.
KEGGiath:AT3G29320.

Organism-specific databases

TAIRiAT3G29320.

Phylogenomic databases

eggNOGiKOG2099. Eukaryota.
COG0058. LUCA.
HOGENOMiHOG000278444.
InParanoidiQ9LIB2.
KOiK00688.
OMAiATYEYYN.
OrthoDBiEOG093601NB.
PhylomeDBiQ9LIB2.

Enzyme and pathway databases

BioCyciARA:AT3G29320-MONOMER.
MetaCyc:AT3G29320-MONOMER.
ReactomeiR-ATH-70221. Glycogen breakdown (glycogenolysis).

Miscellaneous databases

PROiQ9LIB2.

Gene expression databases

ExpressionAtlasiQ9LIB2. baseline and differential.
GenevisibleiQ9LIB2. AT.

Family and domain databases

InterProiIPR011833. Glycg_phsphrylas.
IPR000811. Glyco_trans_35.
[Graphical view]
PANTHERiPTHR11468. PTHR11468. 2 hits.
PfamiPF00343. Phosphorylase. 1 hit.
[Graphical view]
PIRSFiPIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMsiTIGR02093. P_ylase. 1 hit.
PROSITEiPS00102. PHOSPHORYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHS1_ARATH
AccessioniPrimary (citable) accession number: Q9LIB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.