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Protein

Alpha-glucan phosphorylase 1

Gene

PHS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties (By similarity). May be not required for the degradation of starch, but the phosphorolysis of starch may play an important role in water stress tolerance.By similarity1 Publication

Catalytic activityi

((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.

Cofactori

GO - Molecular functioni

GO - Biological processi

  • glycogen catabolic process Source: GO_Central
  • response to temperature stimulus Source: TAIR
  • response to water deprivation Source: TAIR

Keywordsi

Molecular functionAllosteric enzyme, Glycosyltransferase, Transferase
Biological processCarbohydrate metabolism
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT3G29320-MONOMER
MetaCyc:AT3G29320-MONOMER
ReactomeiR-ATH-6798695 Neutrophil degranulation
R-ATH-70221 Glycogen breakdown (glycogenolysis)

Protein family/group databases

CAZyiGT35 Glycosyltransferase Family 35

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-glucan phosphorylase 1 (EC:2.4.1.1)
Short name:
AtPHS1
Alternative name(s):
Alpha-glucan phosphorylase, L isozyme
Starch phosphorylase L
Gene namesi
Name:PHS1
Ordered Locus Names:At3g29320
ORF Names:MUO10.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G29320
TAIRilocus:2093787 AT3G29320

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Small white lesions on the tips or margins of fully expanded leaves.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 63ChloroplastSequence analysisAdd BLAST63
ChainiPRO_000042084364 – 962Alpha-glucan phosphorylase 1Add BLAST899

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei808N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ9LIB2
PRIDEiQ9LIB2
ProMEXiQ9LIB2

PTM databases

iPTMnetiQ9LIB2

Expressioni

Gene expression databases

ExpressionAtlasiQ9LIB2 baseline and differential
GenevisibleiQ9LIB2 AT

Interactioni

Protein-protein interaction databases

BioGridi7919, 2 interactors
IntActiQ9LIB2, 1 interactor
STRINGi3702.AT3G29320.1

Structurei

3D structure databases

ProteinModelPortaliQ9LIB2
SMRiQ9LIB2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi544 – 549Poly-Glu6

Sequence similaritiesi

Belongs to the glycogen phosphorylase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2099 Eukaryota
COG0058 LUCA
HOGENOMiHOG000278444
InParanoidiQ9LIB2
KOiK00688
OMAiWLEMSIN
OrthoDBiEOG093601NB
PhylomeDBiQ9LIB2

Family and domain databases

CDDicd04300 GT1_Glycogen_Phosphorylase, 1 hit
InterProiView protein in InterPro
IPR011833 Glycg_phsphrylas
IPR000811 Glyco_trans_35
IPR035090 Pyridoxal_P_attach_site
PANTHERiPTHR11468 PTHR11468, 1 hit
PfamiView protein in Pfam
PF00343 Phosphorylase, 1 hit
PIRSFiPIRSF000460 Pprylas_GlgP, 1 hit
TIGRFAMsiTIGR02093 P_ylase, 1 hit
PROSITEiView protein in PROSITE
PS00102 PHOSPHORYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LIB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTMRISGVS TGAEVLIQCN SLSSLVSRRC DDGKWRTRMF PARNRDLRPS
60 70 80 90 100
PTRRSFLSVK SISSEPKAKV TDAVLDSEQE VFISSMNPFA PDAASVASSI
110 120 130 140 150
KYHAEFTPLF SPEKFELPKA FFATAQSVRD ALIMNWNATY EYYNRVNVKQ
160 170 180 190 200
AYYLSMEFLQ GRALSNAVGN LGLNSAYGDA LKRLGFDLES VASQEPDPAL
210 220 230 240 250
GNGGLGRLAS CFLDSMATLN YPAWGYGLRY KYGLFKQRIT KDGQEEAAED
260 270 280 290 300
WLELSNPWEI VRNDVSYPIK FYGKVVFGSD GKKRWIGGED IVAVAYDVPI
310 320 330 340 350
PGYKTKTTIN LRLWSTKAPS EDFDLSSYNS GKHTEAAEAL FNAEKICFVL
360 370 380 390 400
YPGDESTEGK ALRLKQQYTL CSASLQDIVA RFETRSGGNV NWEEFPEKVA
410 420 430 440 450
VQMNDTHPTL CIPELMRILM DLKGLSWEDA WKITQRTVAY TNHTVLPEAL
460 470 480 490 500
EKWSLELMEK LLPRHVEIIE KIDEELVRTI VSEYGTADPD LLEEKLKAMR
510 520 530 540 550
ILENVELPSA FADVIVKPVN KPVTAKDAQN GVKTEQEEEK TAGEEEEDEV
560 570 580 590 600
IPEPTVEPPK MVRMANLAVV GGHAVNGVAE IHSEIVKQDV FNDFVQLWPE
610 620 630 640 650
KFQNKTNGVT PRRWIRFCNP YLSDIITNWI GTEDWVLNTE KVAELRKFAD
660 670 680 690 700
NEDLQSEWRA AKKKNKLKVV SLIKERTGYT VSPDAMFDIQ IKRIHEYKRQ
710 720 730 740 750
LLNILGIVYR YKKMKEMSAS EREKAFVPRV CIFGGKAFAT YVQAKRIVKF
760 770 780 790 800
ITDVASTINH DPEIGDLLKV IFVPDYNVSV AELLIPASEL SQHISTAGME
810 820 830 840 850
ASGTSNMKFS MNGCVLIGTL DGANVEIREE VGEENFFLFG AKADQIVNLR
860 870 880 890 900
KERAEGKFVP DPTFEEVKKF VGSGVFGSNS YDELIGSLEG NEGFGRADYF
910 920 930 940 950
LVGKDFPSYI ECQEKVDEAY RDQKRWTRMS IMNTAGSFKF SSDRTIHEYA
960
KDIWNIKQVE LP
Length:962
Mass (Da):108,585
Last modified:October 1, 2000 - v1
Checksum:i54B3C4BBB076CFA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP001309 Genomic DNA Translation: BAB02576.1
CP002686 Genomic DNA Translation: AEE77567.1
AY049235 mRNA Translation: AAK83578.1
BT003015 mRNA Translation: AAO23580.1
RefSeqiNP_189578.1, NM_113857.3
UniGeneiAt.50847
At.5745

Genome annotation databases

EnsemblPlantsiAT3G29320.1; AT3G29320.1; AT3G29320
GeneIDi822590
GrameneiAT3G29320.1; AT3G29320.1; AT3G29320
KEGGiath:AT3G29320

Similar proteinsi

Entry informationi

Entry nameiPHS1_ARATH
AccessioniPrimary (citable) accession number: Q9LIB2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health