Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

UDP-glucose 6-dehydrogenase 2

Gene

UGD2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of UDP-glucuronic acid (UDP-GlcA), providing nucleotide sugars for cell-wall polymers. Required for the formation of cell wall ingrowths on the outer cell walls of nematode-induced syncytia.3 Publications

Catalytic activityi

UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.

Enzyme regulationi

Inhibited by UDP-xylose.1 Publication

Kineticsi

  1. KM=43 µM for NAD+1 Publication
  2. KM=123 µM for UDP-glucose1 Publication

    Pathwayi: UDP-alpha-D-glucuronate biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes UDP-alpha-D-glucuronate from UDP-alpha-D-glucose.
    Proteins known to be involved in this subpathway in this organism are:
    1. UDP-glucose 6-dehydrogenase 2 (UGD2), UDP-glucose 6-dehydrogenase 1 (UGD1), UDP-glucose 6-dehydrogenase 4 (UGD4), UDP-glucose 6-dehydrogenase 3 (UGD3)
    This subpathway is part of the pathway UDP-alpha-D-glucuronate biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-alpha-D-glucuronate from UDP-alpha-D-glucose, the pathway UDP-alpha-D-glucuronate biosynthesis and in Nucleotide-sugar biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei33NADBy similarity1
    Binding sitei38NADBy similarity1
    Binding sitei161NADBy similarity1
    Active sitei272NucleophileBy similarity1
    Binding sitei342NADBy similarity1
    Binding sitei447SubstrateBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi8 – 13NADBy similarity6
    Nucleotide bindingi86 – 90NADBy similarity5
    Nucleotide bindingi127 – 128NADBy similarity2
    Nucleotide bindingi272 – 275NADBy similarity4

    GO - Molecular functioni

    • NAD binding Source: InterPro
    • UDP-glucose 6-dehydrogenase activity Source: UniProtKB

    GO - Biological processi

    • carbohydrate metabolic process Source: TAIR
    • cell wall pectin metabolic process Source: TAIR
    • glycosaminoglycan biosynthetic process Source: GO_Central
    • UDP-glucuronate biosynthetic process Source: UniProtKB

    Keywordsi

    Molecular functionOxidoreductase
    LigandNAD

    Enzyme and pathway databases

    BioCyciARA:AT3G29360-MONOMER
    BRENDAi1.1.1.22 399
    ReactomeiR-ATH-173599 Formation of the active cofactor, UDP-glucuronate
    UniPathwayiUPA00038; UER00491

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucose 6-dehydrogenase 2 (EC:1.1.1.22)
    Short name:
    UDP-Glc dehydrogenase 2
    Short name:
    UDP-GlcDH 2
    Short name:
    UDPGDH 2
    Alternative name(s):
    At-UGD2
    Gene namesi
    Name:UGD2
    Synonyms:UGD
    Ordered Locus Names:At3g29360
    ORF Names:MUO10.18, MUO10_6
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 3

    Organism-specific databases

    AraportiAT3G29360
    TAIRilocus:2093827 AT3G29360

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype. Displays smaller nematode-induced syncytia. The UGD2 UGD3 double mutant displays a strong dwarf phenotype and often develops seedlings with severe root defects; cell walls have an altered sugar composition (PubMed:21949134). Ugd2 and ugd3 double mutants display abnormal nematode-induced syncytia (PubMed:22848518).2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003120271 – 480UDP-glucose 6-dehydrogenase 2Add BLAST480

    Proteomic databases

    PaxDbiQ9LIA8
    PRIDEiQ9LIA8

    PTM databases

    iPTMnetiQ9LIA8

    Expressioni

    Tissue specificityi

    Preferentially expressed in roots.1 Publication

    Developmental stagei

    Restricted expression to the primary root in young seedlings. Later detected in hypocotyl, leaves but with the strongest expression in the root system.2 Publications

    Gene expression databases

    ExpressionAtlasiQ9LIA8 baseline and differential
    GenevisibleiQ9LIA8 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi7923, 1 interactor
    IntActiQ9LIA8, 1 interactor
    STRINGi3702.AT3G29360.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LIA8
    SMRiQ9LIA8
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni157 – 161Substrate bindingBy similarity5
    Regioni216 – 223Substrate bindingBy similarity8
    Regioni256 – 269Substrate bindingBy similarityAdd BLAST14
    Regioni334 – 335Substrate bindingBy similarity2

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG2666 Eukaryota
    COG1004 LUCA
    HOGENOMiHOG000153773
    InParanoidiQ9LIA8
    KOiK00012
    OMAiWKQVIRI
    OrthoDBiEOG093607HD
    PhylomeDBiQ9LIA8

    Family and domain databases

    InterProiView protein in InterPro
    IPR008927 6-PGluconate_DH-like_C_sf
    IPR036291 NAD(P)-bd_dom_sf
    IPR017476 UDP-Glc/GDP-Man
    IPR014027 UDP-Glc/GDP-Man_DH_C
    IPR036220 UDP-Glc/GDP-Man_DH_C_sf
    IPR014026 UDP-Glc/GDP-Man_DH_dimer
    IPR001732 UDP-Glc/GDP-Man_DH_N
    IPR028356 UDPglc_DH_euk
    PfamiView protein in Pfam
    PF00984 UDPG_MGDP_dh, 1 hit
    PF03720 UDPG_MGDP_dh_C, 1 hit
    PF03721 UDPG_MGDP_dh_N, 1 hit
    PIRSFiPIRSF500133 UDPglc_DH_euk, 1 hit
    PIRSF000124 UDPglc_GDPman_dh, 1 hit
    SMARTiView protein in SMART
    SM00984 UDPG_MGDP_dh_C, 1 hit
    SUPFAMiSSF48179 SSF48179, 1 hit
    SSF51735 SSF51735, 1 hit
    SSF52413 SSF52413, 1 hit
    TIGRFAMsiTIGR03026 NDP-sugDHase, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9LIA8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVKICCIGAG YVGGPTMAVI ALKCPDVEVA VVDISVPRIN AWNSDTLPIY
    60 70 80 90 100
    EPGLDDVVKQ CRGKNLFFST DVEKHVREAD IVFVSVNTPT KTRGLGAGKA
    110 120 130 140 150
    ADLTYWESAA RMIADVSVSD KIVVEKSTVP VKTAEAIEKI LTHNSKGIKF
    160 170 180 190 200
    QILSNPEFLA EGTAIKDLFN PDRVLIGGRE TPEGFKAVQT LKNVYAHWVP
    210 220 230 240 250
    EGQIITTNLW SAELSKLAAN AFLAQRISSV NAMSALCEAT GADVTQVSYA
    260 270 280 290 300
    VGTDSRIGPK FLNSSVGFGG SCFQKDILNL VYICECNGLP EVAEYWKQVI
    310 320 330 340 350
    KINDYQKSRF VNRVVSSMFN SVSNKKIAVL GFAFKKDTGD TRETPAIDVC
    360 370 380 390 400
    KGLLEDKARL SIYDPQVTED QIQRDLSMNK FDWDHPLHLQ PMSPTTVKQV
    410 420 430 440 450
    TVTWDAYEAT KDAHGICIMT EWDEFKNLDF QKIFDNMQKP AFVFDGRNIM
    460 470 480
    NLQKLREIGF IVYSIGKPLD DWLKDMPAVA
    Length:480
    Mass (Da):53,173
    Last modified:October 1, 2000 - v1
    Checksum:iD6281D20442CBADF
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti12V → I in AAL11570 (PubMed:14593172).Curated1
    Sequence conflicti178G → R in AAL11570 (PubMed:14593172).Curated1
    Sequence conflicti193N → D in AAM67208 (Ref. 7) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AP001309 Genomic DNA Translation: BAB02581.1
    CP002686 Genomic DNA Translation: AEE77573.1
    CP002686 Genomic DNA Translation: AEE77574.1
    AF424576 mRNA Translation: AAL11570.1
    BT021126 mRNA Translation: AAX22261.1
    AK226539 mRNA Translation: BAE98678.1
    BT029164 mRNA Translation: ABJ17099.1
    AY088902 mRNA Translation: AAM67208.1
    RefSeqiNP_001030792.1, NM_001035715.1
    NP_189582.1, NM_113861.4
    UniGeneiAt.22975

    Genome annotation databases

    EnsemblPlantsiAT3G29360.1; AT3G29360.1; AT3G29360
    AT3G29360.2; AT3G29360.2; AT3G29360
    GeneIDi822594
    GrameneiAT3G29360.1; AT3G29360.1; AT3G29360
    AT3G29360.2; AT3G29360.2; AT3G29360
    KEGGiath:AT3G29360

    Similar proteinsi

    Entry informationi

    Entry nameiUGDH2_ARATH
    AccessioniPrimary (citable) accession number: Q9LIA8
    Secondary accession number(s): Q8L8N1, Q944R8
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
    Last sequence update: October 1, 2000
    Last modified: April 25, 2018
    This is version 141 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health