Reviewed, UniProtKB/Swiss-Prot Q9LIA8 (UGDH1_ARATH)

Names and origin

Protein names

Recommended name:
    Probable UDP-glucose 6-dehydrogenase 1
      Short name=UDP-Glc dehydrogenase 1
      Short name=UDP-GlcDH 1
      Short name=UDPGDH 1
    EC=1.1.1.22

Gene names

Name:	UGD1
Ordered Locus Names:	At3g29360
ORF Names:	MUO10_6, MUO10.18

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Provides UDP-glucuronic acid for hemicellulose precursors By similarity.
Catalytic activity	UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.
Pathway	Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.
Post-translational modification	Phosphorylation level varies significantly during early response to general elicitors.
Sequence similarities	Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: TAIR nucleus Inferred from direct assay. Source: TAIR
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro UDP-glucose 6-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 480	480	Probable UDP-glucose 6-dehydrogenase 1		PRO_0000312027
Regions
	Nucleotide binding	8 – 13	6	NAD By similarity
	Nucleotide binding	86 – 90	5	NAD By similarity
	Nucleotide binding	127 – 128	2	NAD By similarity
	Nucleotide binding	272 – 275	4	NAD By similarity
	Region	157 – 161	5	Substrate binding By similarity
	Region	216 – 223	8	Substrate binding By similarity
	Region	256 – 269	14	Substrate binding By similarity
	Region	334 – 335	2	Substrate binding By similarity
Sites
	Active site	272	1	Nucleophile By similarity
	Binding site	33	1	NAD By similarity
	Binding site	38	1	NAD By similarity
	Binding site	161	1	NAD By similarity
	Binding site	342	1	NAD By similarity
	Binding site	447	1	Substrate By similarity
Amino acid modifications
	Modified residue	393	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	12	1	V → I in AAL11570. Ref.2
	Sequence conflict	178	1	G → R in AAL11570. Ref.2
	Sequence conflict	193	1	N → D in AAM67208. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9LIA8-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D6281D20442CBADF	FASTA	480	53,173
	10 20 30 40 50 60 MVKICCIGAG YVGGPTMAVI ALKCPDVEVA VVDISVPRIN AWNSDTLPIY EPGLDDVVKQ 70 80 90 100 110 120 CRGKNLFFST DVEKHVREAD IVFVSVNTPT KTRGLGAGKA ADLTYWESAA RMIADVSVSD 130 140 150 160 170 180 KIVVEKSTVP VKTAEAIEKI LTHNSKGIKF QILSNPEFLA EGTAIKDLFN PDRVLIGGRE 190 200 210 220 230 240 TPEGFKAVQT LKNVYAHWVP EGQIITTNLW SAELSKLAAN AFLAQRISSV NAMSALCEAT 250 260 270 280 290 300 GADVTQVSYA VGTDSRIGPK FLNSSVGFGG SCFQKDILNL VYICECNGLP EVAEYWKQVI 310 320 330 340 350 360 KINDYQKSRF VNRVVSSMFN SVSNKKIAVL GFAFKKDTGD TRETPAIDVC KGLLEDKARL 370 380 390 400 410 420 SIYDPQVTED QIQRDLSMNK FDWDHPLHLQ PMSPTTVKQV TVTWDAYEAT KDAHGICIMT 430 440 450 460 470 480 EWDEFKNLDF QKIFDNMQKP AFVFDGRNIM NLQKLREIGF IVYSIGKPLD DWLKDMPAVA

References

[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Arabidopsis ORF Clone." Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[7]	"Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis." Benschop J.J., Mohammed S., O'Flaherty M., Heck A.J.R., Slijper M., Menke F.L.H. Mol. Cell. Proteomics 6:1198-1214(2007) [PubMed: 17317660] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-393, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP001309 Genomic DNA. Translation: BAB02581.1. AF424576 mRNA. Translation: AAL11570.1. AY088902 mRNA. Translation: AAM67208.1. BT021126 mRNA. Translation: AAX22261.1. AK226539 mRNA. Translation: BAE98678.1. BT029164 mRNA. Translation: ABJ17099.1.
IPI	IPI00545262.
RefSeq	NP_001030792.1. NP_189582.1.
UniGene	At.22975
3D structure databases
SMR	Q9LIA8. Positions 3-467.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LIA8.
Proteomic databases
PRIDE	Q9LIA8.
Genome annotation databases
GeneID	822594.
GenomeReviews	Gene locus AT3G29360 in contig BA000014_GR.
KEGG	ath:AT3G29360.
NMPDR	fig|3702.1.peg.15250.
Organism-specific databases
TAIR	At3g29360.
Phylogenomic databases
eggNOG	KOG2666.
HOGENOM	HBG400967.
InParanoid	Q9LIA8.
OMA	VELNTWQ.
PhylomeDB	Q9LIA8.
Enzyme and pathway databases
BRENDA	1.1.1.22. 302.
Gene expression databases
Genevestigator	Q9LIA8.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR016040. NAD(P)-bd_dom. IPR017476. Nucleotide_sugar_DH. IPR014027. UDP-Glc/GDP-Man_DH_C. IPR014026. UDP-Glc/GDP-Man_DH_dimer. IPR014028. UDP-Glc/GDP-Man_DH_dimer-bd. IPR001732. UDP-Glc/GDP-Man_DH_N. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:3.40.50.1870. UDP-Glc/GDP-Man_DH_C. 1 hit.
PANTHER	PTHR11374. UDPG_MGDP_DH_Creg. 1 hit.
Pfam	PF00984. UDPG_MGDP_dh. 1 hit. PF03720. UDPG_MGDP_dh_C. 1 hit. PF03721. UDPG_MGDP_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR03026. NDP-sugDHase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UGDH1_ARATH

Accession

Primary (citable) accession number: Q9LIA8
Secondary accession number(s): Q8L8N1, Q944R8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)