Phospholipid-transporting ATPase 10 - Arabidopsis thaliana (Mouse-ear cress)

Names and origin

Protein names

Recommended name:
    Phospholipid-transporting ATPase 10
      Short name=AtALA10
    EC=3.6.3.1
Alternative name(s):
    Aminophospholipid flippase 10

Gene names

Name:	ALA10
Ordered Locus Names:	At3g25610
ORF Names:	T5M7.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	1202 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Involved in transport of phospholipids Potential.
Catalytic activity	ATP + H₂O + phospholipid(In) = ADP + phosphate + phospholipid(Out).
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IV subfamily.

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Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro phospholipid transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Ref.2 Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid-translocating ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1202

1202

Phospholipid-transporting ATPase 10

PRO_0000046394

Regions

Topological domain

1 – 73

73

Cytoplasmic Potential

Transmembrane

74 – 95

22

Potential

Topological domain

96 – 99

4

Extracellular Potential

Transmembrane

100 – 122

23

Potential

Topological domain

123 – 305

183

Cytoplasmic Potential

Transmembrane

306 – 327

22

Potential

Topological domain

328 – 364

37

Extracellular Potential

Transmembrane

365 – 382

18

Potential

Topological domain

383 – 920

538

Cytoplasmic Potential

Transmembrane

921 – 940

20

Potential

Topological domain

941 – 954

14

Extracellular Potential

Transmembrane

955 – 974

20

Potential

Topological domain

975 – 1004

30

Cytoplasmic Potential

Transmembrane

1005 – 1027

23

Potential

Topological domain

1028 – 1040

13

Extracellular Potential

Transmembrane

1041 – 1063

23

Potential

Topological domain

1064 – 1069

6

Cytoplasmic Potential

Transmembrane

1070 – 1090

21

Potential

Topological domain

1091 – 1107

17

Extracellular Potential

Transmembrane

1108 – 1132

25

Potential

Topological domain

1133 – 1202

70

Cytoplasmic Potential

Sites

Active site

430

1

4-aspartylphosphate intermediate By similarity

Metal binding

865

1

Magnesium By similarity

Metal binding

869

1

Magnesium By similarity

Amino acid modifications

Modified residue

31

1

Phosphoserine Ref.2 Ref.3

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9LI83-1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 5930D14DB7942547
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FASTA

1,202

136,279

        10         20         30         40         50         60 
MAGPSRRRRR LHLSKIYSYT CGKSSFQEDH SNIGGPGFSR VVYCNEPGSP AAERRNYAGN 

        70         80         90        100        110        120 
YVRSTKYTVA SFFPKSLFEQ FRRVANFYFL VTGILSLTDL SPYGAVSALL PLALVISATM 

       130        140        150        160        170        180 
VKEGIEDWRR KQQDIEVNNR KVKVHDGNGI FRQEEWRNLR VGDIVRVEKD EFFPADLLLL 

       190        200        210        220        230        240 
SSSYEDSVCY VETMNLDGET NLKVKQGLEA TSSLLNQDSD FKDFRGVVRC EDPNVNLYVF 

       250        260        270        280        290        300 
VGTLALEEER FPLSIQQILL RDSKLRNTEY VYGAVVFTGH DTKVIQNSTD PPSKRSRIER 

       310        320        330        340        350        360 
TMDKIIYLMF GLVFLMSFVG SIIFGVETRE DKVKNGRTER WYLKPDDADI FFDPERAPMA 

       370        380        390        400        410        420 
AIYHFFTATM LYSYFIPISL YVSIEIVKVL QSIFINRDIH MYYEETDKPA QARTSNLNEE 

       430        440        450        460        470        480 
LGMVDTILSD KTGTLTCNSM EFIKCSIAGK AYGRGITEVE RAMAVRSGGS PLVNEDLDVV 

       490        500        510        520        530        540 
VDQSGPKVKG FNFEDERVMN GNWVRQPEAA VLQKFFRLLA VCHTAIPETD EESGNVSYEA 

       550        560        570        580        590        600 
ESPDEAAFVV AAREFGFEFF NRTQNGISFR ELDLVSGEKV ERVYRLLNVL EFNSTRKRMS 

       610        620        630        640        650        660 
VIVRDDDGKL LLLSKGADNV MFERLAKNGR QFEAKTQEHV NQYADAGLRT LVLAYREVDE 

       670        680        690        700        710        720 
NEYIEFNKSF NEAKASVSED REALIDEITD KMERDLILLG ATAVEDKLQN GVPECIDKLA 

       730        740        750        760        770        780 
QAGIKIWVLT GDKMETAINI GFASSLLRQE MKQIIINLET PQIKSLEKSG GKDEIELASR 

       790        800        810        820        830        840 
ESVVMQLQEG KALLAASGAS SEAFALIIDG KSLTYALEDE IKKMFLDLAT SCASVICCRS 

       850        860        870        880        890        900 
SPKQKALVTR LVKSGTGKTT LAIGDGANDV GMLQEADIGV GISGVEGMQA VMSSDIAIAQ 

       910        920        930        940        950        960 
FRYLERLLLV HGHWCYSRIA SMICYFFYKN ITFGVTVFLY EAYTSFSGQP AYNDWFLSLF 

       970        980        990       1000       1010       1020 
NVFFSSLPVI ALGVFDQDVS ARFCYKFPLL YQEGVQNILF SWKRIIGWMF NGFISALAIF 

      1030       1040       1050       1060       1070       1080 
FLCKESLKHQ LFDPDGKTAG REILGGTMYT CVVWVVNLQM ALSISYFTWV QHIVIWGSIA 

      1090       1100       1110       1120       1130       1140 
FWYIFLMIYG AMTPSFSTDA YMVFLEALAP APSYWLTTLF VMIFALIPYF VYKSVQMRFF 

      1150       1160       1170       1180       1190       1200 
PKYHQMIQWI RYEGHSNDPE FVEMVRQRSI RPTTVGYTAR RAASVRRSAR FHDQIYKDLV 


GV

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References

[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry." Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C. Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed: 14506206] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31, MASS SPECTROMETRY.
[3]	"Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database." Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C. Plant Cell 16:2394-2405(2004) [PubMed: 15308754] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AP001313 Genomic DNA. Translation: BAB03080.1.
IPI	IPI00525000.
RefSeq	NP_189189.1.
UniGene	At.49391
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9LI83.
Genome annotation databases
GeneID	822148.
GenomeReviews	Gene locus AT3G25610 in contig BA000014_GR.
KEGG	ath:AT3G25610.
NMPDR	fig\|3702.1.peg.14817.
Organism-specific databases
TAIR	At3g25610.
Phylogenomic databases
OMA	RIASMIC.
Enzyme and pathway databases
BRENDA	3.6.3.1. 302.
Gene expression databases
ArrayExpress	Q9LI83.
Genevestigator	Q9LI83.
GermOnline	AT3G25610. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-reg. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR006539. ATPase_P-typ_Plipid-transl. IPR005834. Dehalogen-like_hydro. IPR013200. HAD-SF_hydro-like_3. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. PF08282. Hydrolase_3. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
TIGRFAMs	TIGR01652. ATPase-Plipid. 1 hit. TIGR01494. ATPase_P-type. 4 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

ALA10_ARATH

Accession

Primary (citable) accession number: Q9LI83

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents