Q9LHQ7 (MCES1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
mRNA cap guanine-N7 methyltransferase 1
EC=2.1.1.56

Alternative name(s):
mRNA (guanine-N(7)-)-methyltransferase 1
mRNA cap methyltransferase 1

Gene names

Ordered Locus Names:	At3g20650
ORF Names:	F3H11.3

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC By similarity.
Catalytic activity	S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. mRNA cap methyltransferase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LHQ7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	mRNA cap guanine-N7 methyltransferase 1		PRO_0000248331
Regions
	Region	70 – 71	2	mRNA cap binding By similarity
Sites
	Binding site	74	1	S-adenosyl-L-methionine By similarity
	Binding site	92	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	95	1	mRNA cap By similarity
	Binding site	101	1	mRNA cap By similarity
	Binding site	114	1	S-adenosyl-L-methionine By similarity
	Binding site	126	1	mRNA cap By similarity
	Binding site	150	1	S-adenosyl-L-methionine By similarity
	Binding site	172	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	176	1	mRNA cap By similarity
	Binding site	264	1	mRNA cap By similarity
	Binding site	333	1	mRNA cap By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 48D4DEA9DB81575D	FASTA	370	42,238
	10 20 30 40 50 60 MKRGFSDSPS SSAPPPSSRF KSNPEGDSQF LEDETTKNFA RKVADHYSRR TNQTLEEREA 70 80 90 100 110 120 SPIIHLKKLN NWIKSVLIQL YARPDDAVLD LACGKGGDLI KWDKARIGYY VGIDIAEGSI 130 140 150 160 170 180 EDCRTRYNGD ADHHQRRKKF SFPSRLLCGD CFEVELDKIL EEDAPFDICS CQFAMHYSWT 190 200 210 220 230 240 TEARARRALA NVSALLRPGG VFIGTMPDAN VIIKKLREAE GLEIGNSVYW IRFGEEYSQK 250 260 270 280 290 300 KFKSSSPFGI EYVFHLEDAV DCPEWIVPFN VFKSLAEEYD LELVFVKNSH EFVHEYMKKP 310 320 330 340 350 360 EFVELMRRLG ALGDGSNDQS TLSADEWEAA YLYLSFVLRK RGESDGARRS GRRKNGKMNL 370 SKDDVLYIDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP002034 Genomic DNA. Translation: BAB02241.1. CP002686 Genomic DNA. Translation: AEE76407.1. BT005804 mRNA. Translation: AAO64206.1. BT006067 mRNA. Translation: AAP04052.1. AK228381 mRNA. Translation: BAF00319.1.
IPI	IPI00524860.
RefSeq	NP_188701.2. NM_112956.5.
UniGene	At.43009.
3D structure databases
HSSP	HSSP built from PDB template 1RI5 based on UniProtKB Q8SR66.
ProteinModelPortal	Q9LHQ7.
SMR	Q9LHQ7. Positions 63-341.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LHQ7.
Proteomic databases
PRIDE	Q9LHQ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G20650.1; AT3G20650.1; AT3G20650.
GeneID	821612.
GenomeReviews	Gene locus AT3G20650 in contig BA000014_GR.
KEGG	ath:AT3G20650.
NMPDR	fig|3702.1.peg.14297.
Organism-specific databases
GeneFarm	4528. 451.
TAIR	At3g20650.
Phylogenomic databases
eggNOG	KOG1975.
GeneTree	EPGT00070000029441.
HOGENOM	HBG377892.
InParanoid	Q9LHQ7.
OMA	FAFHYAC.
PhylomeDB	Q9LHQ7.
ProtClustDB	CLSN2680850.
Gene expression databases
ArrayExpress	Q9LHQ7.
Genevestigator	Q9LHQ7.
GermOnline	AT3G20650. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR016899. mRNA_G-N7_MeTrfase. IPR004971. Pox_MCEL. [Graphical view]
KO	K00565.
Pfam	PF03291. Pox_MCEL. 1 hit. [Graphical view]
PIRSF	PIRSF028762. ABD1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCES1_ARATH

Accession

Primary (citable) accession number: Q9LHQ7
Secondary accession number(s): Q3EB33

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	October 1, 2000
Last modified:	November 16, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program