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Protein

Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5

Gene

LPAT5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

May convert lysophosphatidic acid (LPA) into phosphatidic acid by incorporating acyl moiety at the 2 position (By similarity). Has no activity when expressed in bacteria or yeast.By similarity

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase, chloroplastic (ATS1), Glycerol-3-phosphate acyltransferase 1 (GPAT1), Probable glycerol-3-phosphate acyltransferase 3 (GPAT3), Glycerol-3-phosphate acyltransferase 5 (GPAT5), Glycerol-3-phosphate acyltransferase, chloroplastic (AXX17_At1g32940), Probable glycerol-3-phosphate acyltransferase 2 (GPAT2), Glycerol-3-phosphate acyltransferase 7 (GPAT7), Probable glycerol-3-phosphate acyltransferase 8 (GPAT8)
  2. 1-acyl-sn-glycerol-3-phosphate acyltransferase 3 (LPAT3), 1-acyl-sn-glycerol-3-phosphate acyltransferase 1, chloroplastic (LPAT1), Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 (LPAT5), Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 4 (LPAT4), 1-acyl-sn-glycerol-3-phosphate acyltransferase 2 (LPAT2)
  3. Phosphatidate cytidylyltransferase 1 (CDS1), Phosphatidate cytidylyltransferase (AXX17_At1g55730), Phosphatidate cytidylyltransferase 2 (CDS2), Phosphatidate cytidylyltransferase (AXX17_At2g42720), Phosphatidate cytidylyltransferase (AXX17_At4g30920), Phosphatidate cytidylyltransferase (CDS2), Phosphatidate cytidylyltransferase (CDS2), Phosphatidate cytidylyltransferase 3 (CDS3), Phosphatidate cytidylyltransferase 4, chloroplastic (CDS4), Phosphatidate cytidylyltransferase (AXX17_At3g55010), Phosphatidate cytidylyltransferase (AXX17_At4g25970), Phosphatidate cytidylyltransferase 5, chloroplastic (CDS5)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciARA:AT3G18850-MONOMER.
BRENDAi2.3.1.51. 399.
ReactomeiR-ATH-1482798. Acyl chain remodeling of CL.
R-ATH-1483166. Synthesis of PA.
R-ATH-75109. Triglyceride Biosynthesis.
UniPathwayiUPA00557; UER00613.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 (EC:2.3.1.51)
Alternative name(s):
Lysophosphatidyl acyltransferase 5
Gene namesi
Name:LPAT5
Synonyms:LPAAT5
Ordered Locus Names:At3g18850
ORF Names:MCB22.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G18850.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei21 – 41HelicalSequence analysisAdd BLAST21
Transmembranei57 – 77HelicalSequence analysisAdd BLAST21
Transmembranei312 – 332HelicalSequence analysisAdd BLAST21
Transmembranei337 – 357HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002081841 – 375Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5Add BLAST375

Proteomic databases

PaxDbiQ9LHN4.

Expressioni

Tissue specificityi

Widely expressed at low level.1 Publication

Gene expression databases

ExpressionAtlasiQ9LHN4. baseline and differential.
GenevisibleiQ9LHN4. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G18850.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LHN4.
SMRiQ9LHN4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi100 – 105HXXXXD motif6

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1505. Eukaryota.
COG0204. LUCA.
HOGENOMiHOG000005662.
InParanoidiQ9LHN4.
KOiK13513.
OMAiQGHHVNG.
OrthoDBiEOG09360ABC.
PhylomeDBiQ9LHN4.

Family and domain databases

InterProiIPR032098. Acyltransf_C.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF16076. Acyltransf_C. 1 hit.
PF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LHN4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKKSVPNSD KLSLIRVLRG IICLMVLVST AFMMLIFWGF LSAVVLRLFS
60 70 80 90 100
IRYSRKCVSF FFGSWLALWP FLFEKINKTK VIFSGDKVPC EDRVLLIANH
110 120 130 140 150
RTEVDWMYFW DLALRKGQIG NIKYVLKSSL MKLPLFGWAF HLFEFIPVER
160 170 180 190 200
RWEVDEANLR QIVSSFKDPR DALWLALFPE GTDYTEAKCQ RSKKFAAENG
210 220 230 240 250
LPILNNVLLP RTKGFVSCLQ ELSCSLDAVY DVTIGYKTRC PSFLDNVYGI
260 270 280 290 300
EPSEVHIHIR RINLTQIPNQ EKDINAWLMN TFQLKDQLLN DFYSNGHFPN
310 320 330 340 350
EGTEKEFNTK KYLINCLAVI AFTTICTHLT FFSSMIWFRI YVSLACVYLT
360 370
SATHFNLRSV PLVETAKNSL KLVNK
Length:375
Mass (Da):43,490
Last modified:October 1, 2000 - v1
Checksum:i8F6F5DC313A3D704
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP002039 Genomic DNA. Translation: BAB03094.1.
CP002686 Genomic DNA. Translation: AEE76155.1.
CP002686 Genomic DNA. Translation: AEE76156.1.
CP002686 Genomic DNA. Translation: AEE76157.1.
CP002686 Genomic DNA. Translation: AEE76158.1.
AY099849 mRNA. Translation: AAM20700.1.
BT008462 mRNA. Translation: AAP37821.1.
RefSeqiNP_001030724.2. NM_001035647.3. [Q9LHN4-1]
NP_001078183.1. NM_001084714.1. [Q9LHN4-1]
NP_188515.1. NM_112771.5. [Q9LHN4-1]
NP_974335.1. NM_202606.2. [Q9LHN4-1]
UniGeneiAt.8309.

Genome annotation databases

EnsemblPlantsiAT3G18850.1; AT3G18850.1; AT3G18850. [Q9LHN4-1]
AT3G18850.2; AT3G18850.2; AT3G18850. [Q9LHN4-1]
AT3G18850.3; AT3G18850.3; AT3G18850. [Q9LHN4-1]
AT3G18850.4; AT3G18850.4; AT3G18850. [Q9LHN4-1]
GeneIDi821418.
GrameneiAT3G18850.1; AT3G18850.1; AT3G18850.
AT3G18850.2; AT3G18850.2; AT3G18850.
AT3G18850.3; AT3G18850.3; AT3G18850.
AT3G18850.4; AT3G18850.4; AT3G18850.
KEGGiath:AT3G18850.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP002039 Genomic DNA. Translation: BAB03094.1.
CP002686 Genomic DNA. Translation: AEE76155.1.
CP002686 Genomic DNA. Translation: AEE76156.1.
CP002686 Genomic DNA. Translation: AEE76157.1.
CP002686 Genomic DNA. Translation: AEE76158.1.
AY099849 mRNA. Translation: AAM20700.1.
BT008462 mRNA. Translation: AAP37821.1.
RefSeqiNP_001030724.2. NM_001035647.3. [Q9LHN4-1]
NP_001078183.1. NM_001084714.1. [Q9LHN4-1]
NP_188515.1. NM_112771.5. [Q9LHN4-1]
NP_974335.1. NM_202606.2. [Q9LHN4-1]
UniGeneiAt.8309.

3D structure databases

ProteinModelPortaliQ9LHN4.
SMRiQ9LHN4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G18850.1.

Proteomic databases

PaxDbiQ9LHN4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G18850.1; AT3G18850.1; AT3G18850. [Q9LHN4-1]
AT3G18850.2; AT3G18850.2; AT3G18850. [Q9LHN4-1]
AT3G18850.3; AT3G18850.3; AT3G18850. [Q9LHN4-1]
AT3G18850.4; AT3G18850.4; AT3G18850. [Q9LHN4-1]
GeneIDi821418.
GrameneiAT3G18850.1; AT3G18850.1; AT3G18850.
AT3G18850.2; AT3G18850.2; AT3G18850.
AT3G18850.3; AT3G18850.3; AT3G18850.
AT3G18850.4; AT3G18850.4; AT3G18850.
KEGGiath:AT3G18850.

Organism-specific databases

TAIRiAT3G18850.

Phylogenomic databases

eggNOGiKOG1505. Eukaryota.
COG0204. LUCA.
HOGENOMiHOG000005662.
InParanoidiQ9LHN4.
KOiK13513.
OMAiQGHHVNG.
OrthoDBiEOG09360ABC.
PhylomeDBiQ9LHN4.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00613.
BioCyciARA:AT3G18850-MONOMER.
BRENDAi2.3.1.51. 399.
ReactomeiR-ATH-1482798. Acyl chain remodeling of CL.
R-ATH-1483166. Synthesis of PA.
R-ATH-75109. Triglyceride Biosynthesis.

Miscellaneous databases

PROiQ9LHN4.

Gene expression databases

ExpressionAtlasiQ9LHN4. baseline and differential.
GenevisibleiQ9LHN4. AT.

Family and domain databases

InterProiIPR032098. Acyltransf_C.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF16076. Acyltransf_C. 1 hit.
PF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLPAT5_ARATH
AccessioniPrimary (citable) accession number: Q9LHN4
Secondary accession number(s): Q2V3U6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.