Q9LHG8 (ELC_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein ELC
Short name=AtELC

Alternative name(s):
ESCRT-I complex subunit VPS23 homolog 1
Vacuolar protein-sorting-associated protein 23 homolog 1

Gene names

Name:	ELC
Synonyms:	ELCH
Ordered Locus Names:	At3g12400
ORF Names:	T2E22.28

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the ESCRT-I complex (endosomal sorting complex required for transport I), a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs) Mediates the association to the ESCRT-0 complex. May control nuclear division through the microtubule cytoskeleton. Ref.6
Subunit structure	Component of the endosomal sorting required for transport complex I (ESCRT-I), composed of ELC, VPS28 and VPS37. Interacts with VPS28 and VPS37. Binds ubiquitin in vitro. Ref.6
Subcellular location	Early endosome. Late endosome.
Tissue specificity	Expressed in roots, stems, leaves and flowers. Ref.6
Disruption phenotype	Clustered trichomes and multinucleated cells. Ref.6
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily. Contains 1 SB (steadiness box) domain. Contains 1 UEV (ubiquitin E2 variant) domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from mutant phenotype Ref.6. Source: TAIR cellular protein modification process Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW trichome branching Inferred from mutant phenotype Ref.6. Source: TAIR
Cellular_component	ESCRT I complex Inferred from sequence or structural similarity Ref.6. Source: TAIR early endosome Inferred from direct assay Ref.6. Source: TAIR late endosome Inferred from direct assay Ref.6. Source: TAIR
Molecular_function	ubiquitin binding Inferred from direct assay Ref.6. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ELCL	Q9FFY6	4	EBI-3865248,EBI-3865255
VPS36	Q9FF81	3	EBI-3865248,EBI-3865302
VPS37-1	Q9SCP9	4	EBI-3865248,EBI-3865264

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Protein ELC		PRO_0000368020
Regions
	Domain	18 – 162	145	UEV
	Domain	322 – 390	69	SB
	Coiled coil	247 – 291	45	Potential
	Compositional bias	156 – 197	42	Pro-rich

Sequences

	Sequence		Length	Mass (Da)
	Q9LHG8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 659996F9784FDC20	FASTA	398	44,716
	10 20 30 40 50 60 MVPPPSNPQQ VQQFLSSALS QRGPSSVPYE ESNKWLIRQH LLNLISSYPS LEPKTASFMH 70 80 90 100 110 120 NDGRSVNLLQ ADGTIPMPFH GVTYNIPVII WLLESYPRHP PCVYVNPTAD MIIKRPHAHV 130 140 150 160 170 180 TPSGLVSLPY LQNWVYPSSN LVDLVSDLSA AFARDPPLYS RRRPQPPPPS PPTVYDSSLS 190 200 210 220 230 240 RPPSADQSLP RPFPPSPYGG GVSRVQVQHV HHQQQSDDAA EVFKRNAINK MVEMVHSDLV 250 260 270 280 290 300 SMRRAREAEA EELLSLQAGL KRREDELNIG LKEMVEEKET LEQQLQIISM NTDILDSWVR 310 320 330 340 350 360 ENQGKTKNLV DLDVDNAFEC GDTLSKQMLE CTALDLAIED AIYSLDKSFQ DGVVPFDQYL 370 380 390 RNVRLLSREQ FFHRATGSKV RAAQMEVQVA AIAGRLHS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The Arabidopsis elch mutant reveals functions of an ESCRT component in cytokinesis." Spitzer C., Schellmann S., Sabovljevic A., Shahriari M., Keshavaiah C., Bechtold N., Herzog M., Mueller S., Hanisch F.-G., Huelskamp M. Development 133:4679-4689(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH VPS28; VPS37 AND UBIQUITIN, DISRUPTION PHENOTYPE, NOMENCLATURE.
[7]	"Exploring the ESCRTing machinery in eukaryotes." Winter V., Hauser M.-T. Trends Plant Sci. 11:115-123(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP002047 Genomic DNA. Translation: BAB03147.1. AC069474 Genomic DNA. Translation: AAG51025.1. CP002686 Genomic DNA. Translation: AEE75193.1. AY056283 mRNA. Translation: AAL07132.1. AY133779 mRNA. Translation: AAM91713.1. AY087782 mRNA. Translation: AAM65318.1.
IPI	IPI00540832.
RefSeq	NP_566423.1. NM_112075.2.
UniGene	At.17435. At.67881.
3D structure databases
HSSP	HSSP built from PDB template 1KPQ based on UniProtKB Q99816.
ProteinModelPortal	Q9LHG8.
SMR	Q9LHG8. Positions 36-161.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LHG8. 7 interactions.
STRING	3702.AT3G12400.1-P.
Proteomic databases
PaxDb	Q9LHG8.
PRIDE	Q9LHG8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G12400.1; AT3G12400.1; AT3G12400.
GeneID	820419.
KEGG	ath:AT3G12400.
Organism-specific databases
TAIR	At3g12400.
Phylogenomic databases
eggNOG	NOG317261.
HOGENOM	HOG000030046.
InParanoid	Q9LHG8.
KO	K12183.
OMA	MPGMPAV.
PhylomeDB	Q9LHG8.
ProtClustDB	CLSN2687053.
Gene expression databases
Genevestigator	Q9LHG8.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR017916. Steadiness_box. IPR016135. UBQ-conjugating_enzyme/RWD. IPR008883. UEV_N. [Graphical view]
Pfam	PF05743. UEV. 1 hit. PF09454. Vps23_core. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS51312. SB. 1 hit. PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. False negative. PS51322. UEV. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ELC_ARATH

Accession

Primary (citable) accession number: Q9LHG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program