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Protein

Probable mediator of RNA polymerase II transcription subunit 37c

Gene

MED37C

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity
ATP-dependent molecular chaperone that assists folding of unfolded or misfolded proteins under stress conditions. Mediates plastid precursor degradation to prevent cytosolic precursor accumulation, together with the E3 ubiquitin-protein ligase CHIP. Recognizes specific sequence motifs in transit peptides and thereby led to precursor degradation through the ubiquitin-proteasome system. Plays a critical role in embryogenesis.1 Publication
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage (By similarity).By similarity

GO - Molecular functioni

  • ATP binding Source: TAIR
  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  • protein ubiquitination Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to bacterium Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to heat Source: UniProtKB
  • response to high light intensity Source: TAIR
  • response to hydrogen peroxide Source: TAIR
  • response to temperature stimulus Source: TAIR
  • response to virus Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response, Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-333-MONOMER.
ReactomeiR-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-3371568. Attenuation phase.
R-ATH-3371571. HSF1-dependent transactivation.
R-ATH-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mediator of RNA polymerase II transcription subunit 37c
Alternative name(s):
Heat shock 70 kDa protein 4
Heat shock cognate 70 kDa protein 4
Heat shock cognate protein 70-4
Short name:
AtHsc70-4
Heat shock protein 70-4
Short name:
AtHsp70-4
Gene namesi
Name:MED37C
Synonyms:HSC70-4, HSP70, HSP70-4, MED37_2
Ordered Locus Names:At3g12580
ORF Names:T16H11.7, T2E22.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G12580.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • cell wall Source: TAIR
  • cytosol Source: TAIR
  • Golgi apparatus Source: TAIR
  • mitochondrion Source: TAIR
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Abnormal embryogenesis. Defective seedlings with high levels of reactive oxygen species and monoubiquitinated LHCB4 precursors.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 650649Probable mediator of RNA polymerase II transcription subunit 37cPRO_0000397045Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki457 – 457Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Ubiquitinated.1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9LHA8.
PRIDEiQ9LHA8.

PTM databases

iPTMnetiQ9LHA8.

Expressioni

Developmental stagei

Up-regulated during seed maturation.1 Publication

Inductioni

By heat shock, cold, high light treatment and oxidative stress. Up-regulated by virus infection and by pathogen attack (P.syringae).4 Publications

Gene expression databases

GenevisibleiQ9LHA8. AT.

Interactioni

Subunit structurei

Component of the Mediator complex (Probable). Interacts with CHIP. Interacts with HSFA1A/HSF1.Curated3 Publications

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi5772. 22 interactions.
IntActiQ9LHA8. 8 interactions.
MINTiMINT-2584399.
STRINGi3702.AT3G12580.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LHA8.
SMRiQ9LHA8. Positions 6-619.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0101. Eukaryota.
COG0443. LUCA.
HOGENOMiHOG000228135.
InParanoidiQ9LHA8.
KOiK03283.
OMAiRTQCERT.
OrthoDBiEOG093604KP.
PhylomeDBiQ9LHA8.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LHA8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKGEGPAI GIDLGTTYSC VGVWQHDRVE IIANDQGNRT TPSYVAFTDS
60 70 80 90 100
ERLIGDAAKN QVAMNPTNTV FDAKRLIGRR YSDPSVQADK SHWPFKVVSG
110 120 130 140 150
PGEKPMIVVN HKGEEKQFSA EEISSMVLIK MREIAEAFLG SPVKNAVVTV
160 170 180 190 200
PAYFNDSQRQ ATKDAGVISG LNVMRIINEP TAAAIAYGLD KKASSVGEKN
210 220 230 240 250
VLIFDLGGGT FDVSLLTIEE GIFEVKATAG DTHLGGEDFD NRMVNHFVQE
260 270 280 290 300
FKRKNKKDIT GNPRALRRLR TACERAKRTL SSTAQTTIEI DSLFEGIDFY
310 320 330 340 350
TTITRARFEE LNMDLFRKCM EPVEKCLRDA KMDKSSVHDV VLVGGSTRIP
360 370 380 390 400
KVQQLLQDFF NGKELCKSIN PDEAVAYGAA VQAAILSGEG NEKVQDLLLL
410 420 430 440 450
DVTPLSLGLE TAGGVMTVLI PRNTTIPTKK EQIFSTYSDN QPGVLIQVYE
460 470 480 490 500
GERARTKDNN LLGKFELSGI PPAPRGVPQI TVCFDIDANG ILNVSAEDKT
510 520 530 540 550
TGQKNKITIT NDKGRLSKEE IEKMVQEAEK YKAEDEEHKK KVDAKNALEN
560 570 580 590 600
YAYNMRNTIK DEKIASKLDA ADKKKIEDAI DQAIEWLDGN QLAEADEFED
610 620 630 640 650
KMKELESLCN PIIARMYQGA GPDMGGAGGM DDDTPAGGSG GAGPKIEEVD
Length:650
Mass (Da):71,101
Last modified:October 1, 2000 - v1
Checksum:i33381E896461C5B9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti126 – 1261M → I in CAA05547 (Ref. 1) Curated
Sequence conflicti149 – 1491T → I in CAA05547 (Ref. 1) Curated
Sequence conflicti161 – 1611A → G in CAA05547 (Ref. 1) Curated
Sequence conflicti342 – 3421L → V in CAA05547 (Ref. 1) Curated
Sequence conflicti356 – 3561L → V in CAA05547 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002551 mRNA. Translation: CAA05547.1.
AP002055 Genomic DNA. Translation: BAB02269.1.
AC069474 Genomic DNA. Translation: AAG51030.1.
CP002686 Genomic DNA. Translation: AEE75218.1.
AY054183 mRNA. Translation: AAL06844.1.
AY054190 mRNA. Translation: AAL06851.1.
AY059885 mRNA. Translation: AAL24367.1.
PIRiJA0171.
RefSeqiNP_187864.1. NM_112093.2.
UniGeneiAt.47608.

Genome annotation databases

EnsemblPlantsiAT3G12580.1; AT3G12580.1; AT3G12580.
GeneIDi820438.
GrameneiAT3G12580.1; AT3G12580.1; AT3G12580.
KEGGiath:AT3G12580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002551 mRNA. Translation: CAA05547.1.
AP002055 Genomic DNA. Translation: BAB02269.1.
AC069474 Genomic DNA. Translation: AAG51030.1.
CP002686 Genomic DNA. Translation: AEE75218.1.
AY054183 mRNA. Translation: AAL06844.1.
AY054190 mRNA. Translation: AAL06851.1.
AY059885 mRNA. Translation: AAL24367.1.
PIRiJA0171.
RefSeqiNP_187864.1. NM_112093.2.
UniGeneiAt.47608.

3D structure databases

ProteinModelPortaliQ9LHA8.
SMRiQ9LHA8. Positions 6-619.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5772. 22 interactions.
IntActiQ9LHA8. 8 interactions.
MINTiMINT-2584399.
STRINGi3702.AT3G12580.1.

PTM databases

iPTMnetiQ9LHA8.

Proteomic databases

PaxDbiQ9LHA8.
PRIDEiQ9LHA8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G12580.1; AT3G12580.1; AT3G12580.
GeneIDi820438.
GrameneiAT3G12580.1; AT3G12580.1; AT3G12580.
KEGGiath:AT3G12580.

Organism-specific databases

TAIRiAT3G12580.

Phylogenomic databases

eggNOGiKOG0101. Eukaryota.
COG0443. LUCA.
HOGENOMiHOG000228135.
InParanoidiQ9LHA8.
KOiK03283.
OMAiRTQCERT.
OrthoDBiEOG093604KP.
PhylomeDBiQ9LHA8.

Enzyme and pathway databases

BioCyciARA:GQT-333-MONOMER.
ReactomeiR-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-3371568. Attenuation phase.
R-ATH-3371571. HSF1-dependent transactivation.
R-ATH-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiQ9LHA8.

Gene expression databases

GenevisibleiQ9LHA8. AT.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMD37C_ARATH
AccessioniPrimary (citable) accession number: Q9LHA8
Secondary accession number(s): Q9ZS55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.